Is the extraction by Whatman FTA filter matrix technology and sequencing of large ribosomal subunit D1-D2 region sufficient for identification of clinical fungi?

abstract：:The treatment of systemic candidal infection in neutropenic patients continues to be a major problem, and only 20% of patients survive despite treatment with amphotericin B (Amph B). Granulocyte colony-stimulating factor (G-CSF) is a haemopoietic glycoprotein that appears to control the survival, cycle, activation, pr...

journal_title：Mycoses

authors： Hamood M,Bluche PF,De Vroey C,Corazza F,Bujan W,Fondu P

更新日期：1994-03-01 00:00:00

abstract：:A case of cutaneous phaeohyphomycosis caused by Cladosporium cladosporioides in a 50-year-old housewife is described. The clinical presentation was an ecthyma-like crusted lesion on the back of her left hand. Scanning electron microscopy of the culture showed the conidiophores and the limoniform or ellipsoidal conidia...

journal_title：Mycoses

authors： Duquia RP,de Almeida HL Jr,Vettorato G,Rocha NM,de Castro LA

更新日期：2010-11-01 00:00:00

abstract：:The work reported here is the first nationwide, multicenter surveillance study conducted in Korea to obtain data on fluconazole susceptibility of Candida albicans (C. albicans) isolates. A total of 1137 isolates of C. albicans obtained from 17 university hospitals in South Korea during the 6-month period, July through...

journal_title：Mycoses

authors： Lee MK,Kim HR,Kang JO,Kim MN,Kim EC,Kim JS,Kim JJ,Park YJ,Song W,Shin JH,Lee KM,Lee NY,Lee M,Lee WG,Lee CK,Lee HJ,Chang CL,Choi TY

更新日期：2007-03-01 00:00:00

abstract：:The in vitro antifungal activity of D0870 against eight isolates of fluconazole-resistant Candida albicans was compared with that of itraconazole, ketoconazole and miconazole. The colorimetric MTT [3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H tetrazolium bromide] assay was used to assess the antifungal activities. The...

journal_title：Mycoses

authors： Yang HC,Mikami Y,Yazawa K,Taguchi H,Nishimura K,Miyaji M,Branchini ML,Aoki FH,Yamamoto K

更新日期：1998-12-01 00:00:00

abstract：:Dermatophytoses are of worldwide distribution. Epidemiological studies concerning dermatophyte infections have been performed in many countries and differences in the incidence and the aetiological agents have been reported in different geographical locations. This study was undertaken to investigate the prevailing sp...

journal_title：Mycoses

authors： Maraki S,Nioti E,Mantadakis E,Tselentis Y

更新日期：2007-11-01 00:00:00

abstract：:The incidence and prevalence of fungal infections in Tanzania remains unknown. We assessed the annual burden in the general population and among populations at risk. Data were extracted from 2012 reports of the Tanzanian AIDS program, WHO, reports, Tanzanian census, and from a comprehensive PubMed search. We used mode...

journal_title：Mycoses

authors： Faini D,Maokola W,Furrer H,Hatz C,Battegay M,Tanner M,Denning DW,Letang E

更新日期：2015-10-01 00:00:00

abstract：:In this study, 36 extracts derived from 29 plant species selected using an ethnobotanical approach were tested for antifungal activity against a taxonomically diverse group of 13 human pathogenic fungi. We compared the inhibitory characteristics of these plant extracts with those of the commonly used antifungals, amph...

journal_title：Mycoses

authors： Ficker CE,Arnason JT,Vindas PS,Alvarez LP,Akpagana K,Gbéassor M,De Souza C,Smith ML

更新日期：2003-02-01 00:00:00

abstract：:Invasive pulmonary aspergillosis is a serious threat to immunocompromised and critical care patients. Recent detailed guidelines and treatment algorithms lead microbiologists and clinicians in diagnosis and treatment of invasive aspergillosis. Currently, there is no tool available that allows to measure guideline adhe...

journal_title：Mycoses

authors： Cornely OA,Koehler P,Arenz D,C Mellinghoff S

更新日期：2018-11-01 00:00:00

abstract：:Comparative studies were performed to assess the stability and lot-to-lot variation of Blastomyces dermatitidis yeast and mycelial phase lysate antigens. Four lots were prepared from each growth phase of B. dermatitidis strain T-58 (canine isolate) during a 14-month period. Serum specimens from dogs with blastomycosis...

journal_title：Mycoses

authors： Orr ML,Bono JL,Abuodeh RO,Williams SJ,Legendre AM,Scalarone GM

更新日期：1994-06-01 00:00:00

abstract：:The aim was to evaluate the in vitro activity of voriconazole compared with those of amphotericin B, itraconazole and fluconazole against 132 bloodstream isolates of Candida non-albicans and Saccharomyces cerevisiae species. The minimal inhibitory concentrations (MICs) were determined by an adapted National Committee ...

journal_title：Mycoses

authors： Swinne D,Watelle M,Van der Flaes M,Nolard N

更新日期：2004-06-01 00:00:00

abstract：:The mould collection of the Centraalbureau voor Schimmelcultures, Baarn, The Netherlands, was screened for isolates originating from warm-blooded animals. The range of species indicates that distribution of clinically relevant, pathogenic or opportunistic strains over the fungal kingdom is non-random. Some opportunist...

journal_title：Mycoses

authors： de Hoog GS,Tan CS,Stalpers JA,Stegehuis G

更新日期：1992-09-01 00:00:00

abstract：:The localisation of a dermatophytosis on the face is rare. Differential diagnoses include a broad range of dermatological disorders, e. g. contact dermatitis, psoriasis vulgaris, seborrhoeic dermatitis, demodicosis, and polymorphic photo eruptions. Two patients suffering from tinea faciei incognito caused by Trichophy...

journal_title：Mycoses

authors： Nenoff P,Mügge C,Herrmann J,Keller U

更新日期：2007-01-01 00:00:00

abstract：:Non-steroidal anti-inflammatory drugs (NSAIDs) are one of the most common pharmacological agents. They have three primary therapeutic properties including anti-inflammatory, anti-pyretic and analgesic effects. Seven NSAIDs were tested against two species of dermatophytes. Percentage inhibition was determined for effec...

journal_title：Mycoses

authors： Al-Janabi AA

更新日期：2011-09-01 00:00:00

abstract：:Between May 1, 1988 and January 1, 1990, 10 patients were treated for Candida peritonitis. Origins of the infections were lesions and perforations of the gastrointestinal tract. Risk factors promoting the disease were tumours, diabetes mellitus and extensive antibiotic therapy. The mean classification by the APACHE sc...

journal_title：Mycoses

authors： Kujath P,Lerch K,Dämmrich J

更新日期：1990-09-01 00:00:00

abstract：:We compared two phenotypic methods, colony morphotyping on Sabouraudtripheniltetrazolium agar (STTZ) and serotyping, with two genotypic methods, karyotyping and Random Amplified Polymorphic DNA bands obtained by PCR amplification (RAPD-PCR), for strain delineation in 33 Candida clinical isolates and two C. albicans st...

journal_title：Mycoses

authors： Del Castillo L,Bikandi J,Nieto A,Quindós G,Sentandreu R,Pontón J

更新日期：1997-12-01 00:00:00

abstract：:The molecular characterization of Malassezia spp. isolates from animals and humans has not been thoroughly studied. We have analysed the DNA profile by random amplified polymorphic DNA (RAPD)-PCR to compare the genetic diversity between isolates from the external ears of cattle, dogs and humans. The analysis of electr...

journal_title：Mycoses

authors： Duarte ER,Hamdan JS

更新日期：2010-01-01 00:00:00

abstract：:A case of fixed cutaneous sporotrichosis that developed on the extensor aspect of the left wrist of an 83-year-old woman was treated once a week with 200 mg of oral fluconazole. This dermatological lesion healed within 4 months, leaving a scar. No side-effects were seen. At the time of writing, 7 months after the end ...

journal_title：Mycoses

authors： Yoshida M,Hiruma M,Tezuka T

更新日期：1994-07-01 00:00:00

abstract：:The secreted aspartyl proteinases (Saps) of Candida albicans have been implicated as immunodominant antigens and virulence factors associated with adherence and tissue invasion. A hybrid phage displaying the Sap epitope VKYTS was constructed by cloning the corresponding DNA fragments into the pfd88 vector. Similar to ...

journal_title：Mycoses

authors： Yang Q,Su QP,Wang GY,Wen DZ,Zhang YH,Bao HZ,Wang L

更新日期：2007-05-01 00:00:00

abstract：:This is an observational-retrospective study comparing the real-world outcomes associated with posaconazole vs itraconazole as prophylaxis treatments. Two hundred and ninety-three patient admissions attributable to 174 patients were included in the study. Patients were treated with itraconazole (n = 114 admissions; 39...

journal_title：Mycoses

authors： Tormo M,Pérez-Martínez A,Calabuig M,Hernández-Boluda JC,Amat P,Navarro D,Solano C

更新日期：2018-03-01 00:00:00

abstract：:Paracoccidioidomycosis (PCM) is the most prevalent systemic mycosis among immunocompetent patients in Latin America. This study aimed to describe the expansion over time and the geographical distribution of confirmed Neuroparacoccidioidomycosis (NPCM) and PCM cases, and relate it to environmental characteristics such ...

journal_title：Mycoses

authors： de Almeida SM,Salvador GLO,Roza TH,Izycki LF,Dos Santos I,Aragão A,Kulik A,Muro M,Torres LFB,de Noronha L

更新日期：2018-08-01 00:00:00

abstract：:Dermatophyte infections, while not life-threatening, are very common, and there is great interest in developing new antifungal agents. Transcriptional profiling of Trichophyton on keratin has identified some antioxidant genes as induced on this host substrate, including a thioredoxin gene TmTRX1. If thioredoxin is a v...

journal_title：Mycoses

authors： Berdicevsky I,Kaufman G,Newman DJ,Horwitz BA

更新日期：2009-07-01 00:00:00

abstract：:A double-blind trial was undertaken in Karonga District, Northern Malawi comparing Whitfield's cream (3% salicylic acid and 6% benzoylic acid) and clotrimazole cream in the treatment of dermatophyte infections of the skin. To date, 138 patients have been reviewed; 65 of these patients received Whitfield's cream and 73...

journal_title：Mycoses

authors： Pönnighaus JM,Gooskens V,Clayton Y,Mkandawire P,Sterne J

更新日期：1994-01-01 00:00:00

abstract：:From stratum corneum samples of a palmar eczema, a fungus was isolated that developed white colonies with a yellowish dark reverse, suggestive of dermatophytes. The isolate produced numerous chlamydospores and sparse aleuroconidia, was resistant to cycloheximide, grew well on human stratum corneum samples and was posi...

journal_title：Mycoses

authors： Brasch J,Beck-Jendroscheck V,Voss K,Yurkov A,Stchigel AM,Gräser Y

更新日期：2017-07-01 00:00:00

abstract：:A 36-year-old HIV-infected male patient presented with relapsing fever episodes to 39 degrees C, night sweats and weight loss. Computerized tomography of the abdomen showed enlarged multiple lymph nodes. After surgical resection of multiple lymph nodes, disseminated infection with Histoplasma capsulatum was diagnosed....

journal_title：Mycoses

authors： Rieg GK,Shah PM,Helm EB,Just-Nübling G

更新日期：1999-04-01 00:00:00

abstract：:In pooled samples of faeces from 25 pet bird flocks in Thuringia, a high rate of contamination with Cryptococcus neoformans var. neoformans was found. The prevalence of Cr. neoformans in the bird-breeding establishments correlated with the numbers of the different pet bird species in these flocks. The differentiation ...

journal_title：Mycoses

authors： Kielstein P,Hotzel H,Schmalreck A,Khaschabi D,Glawischnig W

更新日期：2000-01-01 00:00:00

abstract：:The opportunistic black yeast are particularly known through the genus Exophiala, characterised by annellidic budding cells. However, this phenotype is polyphyletic within the order Chaetothyriales. Seventeen generic names are available in the family Herpotrichiellaceae, one of which is Exophiala. Future taxonomy will...

journal_title：Mycoses

authors： Moussa TAA,Al-Zahrani HS,Kadasa NMS,Moreno LF,Gerrits van den Ende AHG,de Hoog GS,Al-Hatmi AMS

更新日期：2017-06-01 00:00:00

abstract：:Reconstituted multilayered oesophageal epithelium appears to be a good basis to test the efficacy of voriconazole (VOR) and fluconazole (FLU) in the tissue. The resulting model of a Candida oesophagitis was approaching the in vivo situation. We infected the tissue with 2 x 10(6) cfu of the Candida albicans strain SC53...

journal_title：Mycoses

authors： Bernhardt J,Bernhardt H,Knoke M,Ludwig K

更新日期：2004-08-01 00:00:00

abstract：:The performance of Pastorex Aspergillus, a new latex agglutination test for the detection of circulating galactomannan in the serum of patients with invasive aspergillosis, was evaluated in a blind trial in standardized guinea-pig models of invasive aspergillosis and other invasive mycoses. In these animal models, the...

journal_title：Mycoses

authors： Van Cutsem J,Meulemans L,Van Gerven F,Stynen D

更新日期：1990-02-01 00:00:00

abstract：:An atypical strain of Paracoccidioides brasiliensis was obtained from a patient with disseminated paracoccidioidomycosis. This isolate, which has been maintained in the laboratory for 4 years by successive subcultures (30-40 days) in Fava Netto medium, produces cerebriform colonies characteristic of the yeast phase of...

journal_title：Mycoses

authors： Hamdan JS,Ferrari TC

更新日期：1995-11-01 00:00:00

abstract：:Fungi have been used for a long time in industrial production. Recent developments in biotechnology have lead to an increased awareness of possible health hazards connected with the use of microorganisms in industries. In order to avoid potential risks by appropriate protective measures it is necessary to assess the m...

journal_title：Mycoses

authors： Meinhof W,Lüders A,Finkeldey E,Balter A

更新日期：1996-01-01 00:00:00