Characteristics of dendritic cells in rat liver.

Abstract:

:The properties of rat liver dendritic cells (DC) were analyzed after collagenase digestion of the tissue and enrichment with density gradient centrifugation. The liver macrophages (Kupffer cells) were eliminated by adherence before the gradient centrifugation. The morphology of isolated DC in May-Grunwald-Giemsa (MGG) stained cytocentrifuge preparations resembled that of monocytes with certain dissimilarities. The expression of major histocompatibility complex (MHC) class II antigens (Ia) on DC was analyzed with the Staphylococcus aureus rosette method using a monoclonal antibody. The binding of anti-Ia antibody to rat liver DC was 3 times stronger than to passenger lymphocytes and 10 times stronger than to hepatocytes. All DC were Ia-positive tested with indirect immunofluorescence technique, and none of them were able to phagocytize antibody-coated human red cells. The DC did not express intracytoplasmic lysozyme, or surface Fc-receptors, and they all were negative in alpha-naphtylacetate esterase (ANAE) staining. Thus, although the dendritic cells of rat liver seem to belong to the monocytic series according to morphologic criteria, they were all negative when tested for typical monocyte/macrophage markers. The immunocenic potential of DC was analyzed by testing their ability to prime a naive recipient for graft rejection. The number DC needed for the priming was comparable with the number of spleen lymphocytes needed for an equivalent effect, indicating that the DC were highly immunogenic. The hepatocytes showed practically no immunogenic effect. Thus the immunogenic potential of the tested cells, i.e. their ability to induce accelerated transplant rejection, carries a good correlation with the expression of Ia-antigens on the cell surface.

journal_name

Transplantation

journal_title

Transplantation

authors

Lautenschlager I,Halttunen J,Häyry P

doi

10.1097/00007890-198805000-00019

subject

Has Abstract

pub_date

1988-05-01 00:00:00

pages

936-9

issue

5

eissn

0041-1337

issn

1534-6080

journal_volume

45

pub_type

杂志文章
  • A prospective study investigating the cost effectiveness of intraoperative blood salvage during liver transplantation.

    abstract:BACKGROUND:Adult orthotopic liver transplantation is associated with significant use of allogenic blood products, which places considerable demands on finite resources. This could be reduced by autologous red cell salvage use, and we evaluated its cost effectiveness in this prospective study. METHODS:Intraoperative au...

    journal_title:Transplantation

    pub_type: 杂志文章

    doi:10.1097/01.tp.0000199318.17013.c5

    authors: Phillips SD,Maguire D,Deshpande R,Muiesan P,Bowles MJ,Rela M,Heaton ND

    更新日期:2006-02-27 00:00:00

  • A prospective study of human herpesvirus type 6 detected by polymerase chain reaction after liver transplantation.

    abstract::Human herpesvirus type 6 (HHV-6) causes roseola infantum (exanthema subitum) upon primary infection in young children. Thereafter it persists lifelong in the organism. Like other herpesviruses, HHV-6 can be reactivated in periods of immunosuppression - e.g., after organ transplantation. In order to study the incidence...

    journal_title:Transplantation

    pub_type: 杂志文章

    doi:10.1097/00007890-199602270-00027

    authors: Schmidt CA,Wilbron F,Weiss K,Brinkmann V,Oettle H,Lohmann R,Langrehr JM,Neuhaus P,Siegert W

    更新日期:1996-02-27 00:00:00

  • Cyclosporine A sensitizes the kidney to tubulointerstitial fibrosis induced by renal warm ischemia.

    abstract:BACKGROUND:Renal warm ischemic injury and immunosuppression with cyclosporin A (CsA) may contribute to chronic allograft nephropathy after cadaveric transplantation. This study establishes whether CsA can sensitize the kidney to injury and fibrosis induced by renal warm ischemia (RWI). METHODS:The left kidney of Sprag...

    journal_title:Transplantation

    pub_type: 杂志文章

    doi:10.1097/01.tp.0000113850.34945.dc

    authors: Ahmed A,Huang L,Raftery AT,Ahmed AK,Fahmy H,El Nahas AM,Haylor JL

    更新日期:2004-03-15 00:00:00

  • Antibody testing strategies for deceased donor kidney transplantation after immunomodulatory therapy.

    abstract:BACKGROUND:Immunomodulatory protocols including intravenous immunoglobulin/rituximab (IVIG/R) are employed to decrease anti-human leukocyte antigen (HLA) antibody levels for patients broadly sensitized to HLA and increase chances for transplantation with a compatible deceased donor (DD). The aim of our study was to ide...

    journal_title:Transplantation

    pub_type: 杂志文章

    doi:10.1097/TP.0b013e31821eab8a

    authors: Lai CH,Cao K,Ong G,Naim M,Wang Q,Mirocha J,Vo A,Jordan SC,Reinsmoen NL

    更新日期:2011-07-15 00:00:00

  • Failure of B-cold lymphocytotoxins to enhance cadaveric renal allograft survival.

    abstract::In a three-year period between January 1980 and December 1982, 242 consecutive cadaver transplant recipients in one center were tested before transplantation by cytotoxicity against a random panel of T and B lymphocytes at 5 degrees C and 37 degrees C incubation. They were also tested for HLA-A, B, and DR antigens. Ki...

    journal_title:Transplantation

    pub_type: 杂志文章

    doi:10.1097/00007890-198509000-00006

    authors: Mendez R,Chatterjee SN,Mendez R,Bogaard T

    更新日期:1985-09-01 00:00:00

  • Current status of clinical islet transplantation.

    abstract::Islet transplantation is currently being explored as a treatment for patients with type 1 diabetes. At present, the number of patients becoming insulin-independent is rapidly increasing world-wide applying the transplantation protocol originally described by the group in Edmonton. A hallmark in this procedure is repea...

    journal_title:Transplantation

    pub_type: 杂志文章,评审

    doi:10.1097/01.tp.0000157273.60147.7c

    authors: Korsgren O,Nilsson B,Berne C,Felldin M,Foss A,Kallen R,Lundgren T,Salmela K,Tibell A,Tufveson G

    更新日期:2005-05-27 00:00:00

  • Recurrence of focal-segmental glomerulosclerosis in children after renal transplantation: clinical and genetic aspects.

    abstract::Focal segmental glomerulosclerosis (FSGS) is the primary renal disease in approximately one-tenth of pediatric patients receiving a renal allograft. Recurrence of proteinuria after renal transplantation is observed in approximately 30% of patients and negatively impacts graft survival. Risk factors for recurrence are ...

    journal_title:Transplantation

    pub_type: 杂志文章,评审

    doi:10.1097/01.tp.0000187110.25512.82

    authors: Weber S,Tönshoff B

    更新日期:2005-09-27 00:00:00

  • Posttransplant Lymphoproliferative Disorder in Pediatric Patients: Characteristics of Disease in EBV-seropositive Recipients.

    abstract:BACKGROUND:Patients at greatest risk of posttransplant lymphoproliferative disorder (PTLD) are those who acquire primary Epstein-Barr virus (EBV) infection after solid organ transplantation. The incidence of PTLD among patients who are EBV-seropositive before transplant is lower, and little is known about the differenc...

    journal_title:Transplantation

    pub_type: 杂志文章

    doi:10.1097/TP.0000000000002898

    authors: L'Huillier AG,Dipchand AI,Ng VL,Hebert D,Avitzur Y,Solomon M,Ngan BY,Yeung S,Stephens D,Punnett AS,Barton M,Allen UD

    更新日期:2019-11-01 00:00:00

  • DSA Are Associated With More Graft Injury, More Fibrosis, and Upregulation of Rejection-associated Transcripts in Subclinical Rejection.

    abstract:BACKGROUND:Subclinical T cell-mediated rejection (subTCMR) is commonly found after liver transplantation and has a good short-term prognosis, even when it is left untreated. Donor-specific antibodies (DSA) are putatively associated with a worse prognosis for recipient and graft after liver transplantation. METHODS:To ...

    journal_title:Transplantation

    pub_type: 杂志文章

    doi:10.1097/TP.0000000000003034

    authors: Höfer A,Jonigk D,Hartleben B,Verboom M,Hallensleben M,Hübscher SG,Manns MP,Jaeckel E,Taubert R

    更新日期:2020-03-01 00:00:00

  • Outcomes After Declining Increased Infectious Risk Kidney Offers for Pediatric Candidates in the United States.

    abstract:BACKGROUND:Kidneys from infectious risk donors (IRD) confer substantial survival benefit in adults, yet the benefit of IRD kidneys to pediatric candidates remains unclear in the context of high waitlist prioritization. METHODS:Using 2010-2016 Scientific Registry of Transplant Recipients data, we studied 2417 pediatric...

    journal_title:Transplantation

    pub_type: 杂志文章,多中心研究

    doi:10.1097/TP.0000000000002674

    authors: Bowring MG,Jackson KR,Wasik H,Neu A,Garonzik-Wang J,Durand C,Desai N,Massie AB,Segev DL

    更新日期:2019-12-01 00:00:00

  • Clinically significant peripancreatic fluid collections after simultaneous pancreas-kidney transplantation.

    abstract:BACKGROUND:Peripancreatic fluid collections (PPFC) are a serious complication after simultaneous pancreas-kidney transplantation (SPKTx). METHODS:Retrospective study for all 223 SPKTx performed from December 8, 1996, to October 10, 2011, to evaluate the risk factors (RF) and impact of PPFCs on outcomes was conducted. ...

    journal_title:Transplantation

    pub_type: 杂志文章

    doi:10.1097/TP.0b013e318289c978

    authors: Singh RP,Vrakas G,Hayek S,Hayek S,Anam S,Aqueel M,Olsburgh J,Calder F,Mamode N,Callaghan C,Kessaris N,Pattison J,Hilton R,Koffman G,Taylor JD,Drage MW

    更新日期:2013-05-27 00:00:00

  • Analysis of factors affecting recurrence of hepatocellular carcinoma after liver transplantation with a special focus on inflammation markers.

    abstract:BACKGROUND:Systemic inflammation markers, such as neutrophil-to-lymphocyte ratio (NLR), have recently emerged as the prognostic factors for recurrence of liver tumors. METHODS:We assessed the ability of NLR and of other variables to predict the outcomes of orthotopic liver transplantation (OLT) for hepatocellular carc...

    journal_title:Transplantation

    pub_type: 杂志文章

    doi:10.1097/TP.0b013e3182187cf0

    authors: Bertuzzo VR,Cescon M,Ravaioli M,Grazi GL,Ercolani G,Del Gaudio M,Cucchetti A,D'Errico-Grigioni A,Golfieri R,Pinna AD

    更新日期:2011-06-15 00:00:00

  • Protection of porcine aortic endothelial cells from complement-mediated cell lysis and activation by recombinant human CD59.

    abstract::Discordant xenogeneic organ transplantation is a potential solution to the critical shortage of suitable donor organs. However, clinical application of xenotransplantation with physiologically suitable organs such as those from the pig, is currently limited by the lack of agents to prevent antibody and complement-medi...

    journal_title:Transplantation

    pub_type: 杂志文章

    doi:

    authors: Kennedy SP,Rollins SA,Burton WV,Sims PJ,Bothwell AL,Squinto SP,Zavoico GB

    更新日期:1994-05-27 00:00:00

  • The clinical impact of chronic transplant glomerulopathy in cyclosporine era.

    abstract:BACKGROUND:The clinical impact of chronic transplant glomerulopathy (CTG) on the outcome of kidney allograft receiving calcineurin inhibitors (CNIs) remains uncertain. A retrospective study of renal transplant recipients at Ospedale Maggiore of Milan was undertaken to evaluate the clinical outcome of patients with CTG....

    journal_title:Transplantation

    pub_type: 杂志文章

    doi:10.1097/01.tp.0000181167.88133.d2

    authors: Banfi G,Villa M,Cresseri D,Ponticelli C

    更新日期:2005-11-27 00:00:00

  • Ontogeny of antipig xenoantibody and hyperacute rejection.

    abstract:BACKGROUND:Neonatal primates have been reported to receive pig hearts without hyperacute rejection (HAR). We examined the ontogeny of the anti-pig xenoantibody (XenoAb) and HAR in the neonatal and infant monkeys. METHODS:Twenty-six serum samples from 15 monkeys ages 14-192 days were subjected to hemagglutination titra...

    journal_title:Transplantation

    pub_type: 评论,杂志文章

    doi:10.1097/00007890-200008270-00025

    authors: Kawauchi M,Nakajima J,Endoh M,Oka T,Takamoto S

    更新日期:2000-08-27 00:00:00

  • The impact of transforming growth factor-beta1 gene polymorphism on end-stage renal failure after heart transplantation.

    abstract:BACKGROUND:Nephrotoxicity is a major side effect of calcineurin inhibitors (CNI). Earlier we reported 8% of our heart transplant recipients reaching end-stage renal failure (ESRF). Now, with an extended follow up of 20 years, we re-evaluated the development of ESRF and studied its influence on survival and the impact o...

    journal_title:Transplantation

    pub_type: 杂志文章

    doi:10.1097/01.tp.0000250360.78553.5e

    authors: van de Wetering J,Weimar CH,Balk AH,Roodnat JI,Holweg CT,Baan CC,van Domburg RT,Weimar W

    更新日期:2006-12-27 00:00:00

  • Association between capsule diameter, adequacy of encapsulation, and survival of microencapsulated rat islet allografts.

    abstract::As a consequence of its large volume, a microencapsulated islet graft can be implanted only into the peritoneal cavity. The graft volume can be reduced by using small capsules. However, reduction of the diameter of the capsules holds a certain risk, because with smaller capsules, more islets may be found to protrude f...

    journal_title:Transplantation

    pub_type: 杂志文章

    doi:10.1097/00007890-199610150-00004

    authors: De Vos P,De Haan B,Pater J,Van Schilfgaarde R

    更新日期:1996-10-15 00:00:00

  • Liver transplantation using an organ donor with HELLP syndrome.

    abstract:BACKGROUND:The shortage of organs for liver transplantation has forced transplant centers to expand the donor pool by using donors traditionally labeled as marginal. One such example is liver transplantation using a donor with HELLP syndrome (hemolysis, elevated liver enzymes, and low platelets), a disorder of late pre...

    journal_title:Transplantation

    pub_type: 杂志文章

    doi:10.1097/01.TP.0000101510.01404.F0

    authors: Briceno PJ,Ortiz JA,Manzarbeitia C,Jeon H,Munoz SJ,Rothstein KD,Araya V,Gala I,Reich DJ

    更新日期:2004-01-15 00:00:00

  • Uncontrolled Donation After Circulatory Death: A Unique Opportunity.

    abstract::Uncontrolled donation after circulatory death (uDCD) refers to donation from persons who die following an unexpected and unsuccessfully resuscitated cardiac arrest. Despite the large potential for uDCD, programs of this kind only exist in a reduced number of countries with a limited activity. Barriers to uDCD are of a...

    journal_title:Transplantation

    pub_type: 杂志文章,评审

    doi:10.1097/TP.0000000000003139

    authors: Coll E,Miñambres E,Sánchez-Fructuoso A,Fondevila C,Campo-Cañaveral de la Cruz JL,Domínguez-Gil B

    更新日期:2020-08-01 00:00:00

  • Higher frequency of high-grade rejections in cardiac allograft patients after Quilty B lesions or grade 2/4 rejections.

    abstract:BACKGROUND:To better understand how different histologic patterns of allograft inflammation found on biopsies of human cardiac allografts progress to high-grade rejection, we undertook a statistical analysis of our institutional database to detect statistical patterns among different types of myocardial allograft infla...

    journal_title:Transplantation

    pub_type: 杂志文章

    doi:10.1097/00007890-200206270-00014

    authors: Smith RN,Chang Y,Houser S,Dec GW,Grazette L

    更新日期:2002-06-27 00:00:00

  • Combined liver-kidney transplantation in primary hyperoxaluria type 1. Bone histopathology and oxalate body content.

    abstract::In three patients with end-stage renal failure due to primary hyperoxaluria type 1, successful combined liver-kidney transplantation enabled us to assess the insoluble oxalate pool, which was compared with the histopathological changes observed in iliac crest biopsy specimens. Good correlation was observed between the...

    journal_title:Transplantation

    pub_type: 杂志文章

    doi:10.1097/00007890-199506270-00010

    authors: Toussaint C,Vienne A,De Pauw L,Gelin M,Janssen F,Hall M,Schurmans T,Pasteels JL

    更新日期:1995-06-27 00:00:00

  • Cytokine pattern during rejection and infection after liver transplantation--improvements in postoperative monitoring?

    abstract::Despite improvements in immunosuppression, rejection occurs in 50% of liver transplant patients and may cause significant morbidity. The most frequent cause of death after liver transplantation is severe infection. Determination of the cytokine network may lead to earlier detection of patients at risk for severe rejec...

    journal_title:Transplantation

    pub_type: 杂志文章

    doi:10.1097/00007890-199611270-00011

    authors: Platz KP,Mueller AR,Rossaint R,Steinmüller T,Lemmens HP,Lobeck H,Neuhaus P

    更新日期:1996-11-27 00:00:00

  • Thromboembolism in heart transplantation: role of prothrombin G20210A and factor V Leiden.

    abstract:BACKGROUND:Thromboembolism has been reported as a frequent complication after cardiac transplantation. Many risk factors for thrombosis may explain this, such as metabolic alterations and the use of cyclosporine. In the general population, two single nucleotide polymorphisms (SNPs), factor V Leiden and prothrombin G202...

    journal_title:Transplantation

    pub_type: 杂志文章

    doi:10.1097/01.tp.0000170545.42790.6f

    authors: Miriuka SG,Langman LJ,Evrovski J,Miner SE,Kozuszko S,D'Mello N,Delgado DH,Wong BY,Ross HJ,Cole DE

    更新日期:2005-09-15 00:00:00

  • Serum tumor markers after renal transplantation.

    abstract::The overall incidence of malignancy in renal transplant recipients is 100-fold higher compared with age matched controls. Routine clinical evaluation therefore often includes the determination of serum tumor markers AFP, CA19-9, CEA, CA125, CA15-3, PSA, and calcitonin. We evaluated the specificity and the sensitivity ...

    journal_title:Transplantation

    pub_type: 杂志文章

    doi:10.1097/00007890-199611270-00021

    authors: Oberbauer R,Banyai S,Schmidt A,Kornek G,Scheithauer W,Mayer G

    更新日期:1996-11-27 00:00:00

  • Early TLR4 Blockade Attenuates Sterile Inflammation-mediated Stress in Islets During Isolation and Promotes Successful Transplant Outcomes.

    abstract:BACKGROUND:During the isolation process, pancreatic islets are exposed to an environment of sterile inflammation resulting in an upregulated inflammatory state before transplantation. Toll-like receptor 4 (TLR4) has been identified as a major mediator of sterile inflammation. Therefore, we sought to determine whether e...

    journal_title:Transplantation

    pub_type: 杂志文章

    doi:10.1097/TP.0000000000002287

    authors: Chang CA,Murphy K,Kane RR,Lawrence MC,Naziruddin B

    更新日期:2018-09-01 00:00:00

  • Interstitial pneumonitis following autologous bone marrow transplantation.

    abstract::The incidence of interstitial pneumonitis (IP) was reviewed in 70 consecutive patients who received autologous marrow transplants for hematologic malignancies. All patients were treated with total-body irradiation (TBI), with or without other chemotherapeutic agents. Seven patients (10%) developed IP, 3 were due to cy...

    journal_title:Transplantation

    pub_type: 杂志文章

    doi:10.1097/00007890-198611000-00015

    authors: Pecego R,Hill R,Appelbaum FR,Amos D,Buckner CD,Fefer A,Thomas ED

    更新日期:1986-11-01 00:00:00

  • Isoenzyme characterization in isolated elevation of alkaline phosphatase after liver transplantation in children.

    abstract:BACKGROUND:Isolated transient elevation of alkaline phosphatase (ALP) in the absence of bone or liver disease has been reported in children including liver transplant patients. Specific isoenzyme patterns have been associated with this phenomenon in healthy children but have not been reported after liver transplantatio...

    journal_title:Transplantation

    pub_type: 杂志文章

    doi:10.1097/00007890-200210150-00023

    authors: O'Riordan S,Baker AJ,Sherwood RA

    更新日期:2002-10-15 00:00:00

  • A predictive test for the efficacy of intravenous immunoglobulin in the inhibition of alloantibodies.

    abstract::Preformed recipient HLA-specific antibodies can cause hyperacute rejection of a transplanted kidney if they are directed against mismatched donor HLA antigens. To avoid hyperacute rejection it is essential that recipient antibodies be identified during patient workup for transplantation and HLA antigens to which a pat...

    journal_title:Transplantation

    pub_type: 评论,杂志文章

    doi:10.1097/00007890-200105270-00002

    authors: Martin S

    更新日期:2001-05-27 00:00:00

  • Treatment of graft-versus-host disease by extracorporeal photochemotherapy: a pilot study.

    abstract:BACKGROUND:Graft-versus-host disease (GVHD) is a major complication after bone marrow transplantation, which may be refractory to immunosuppressive drugs. As preliminary case reports suggested that extracorporeal photochemotherapy (ECP) using a Therakos device might be beneficial, we conducted a pilot study to assess t...

    journal_title:Transplantation

    pub_type: 杂志文章

    doi:10.1097/00007890-199707150-00010

    authors: Besnier DP,Chabannes D,Mahé B,Mussini JM,Baranger TA,Muller JY,Milpied N,Esnault VL

    更新日期:1997-07-15 00:00:00

  • Liver procurement without in situ portal perfusion. A safe procedure for more flexible multiple organ harvesting.

    abstract::The outcome after liver transplantation when grafts were retrieved from the donor by the classical aortic and portal cooling (APC) method was compared with the outcome when exclusively aortic in situ perfusion (AC) was used. Retrospectively, 163 donor hepatectomies performed over a 20-month period were reviewed to ana...

    journal_title:Transplantation

    pub_type: 杂志文章

    doi:10.1097/00007890-199405150-00007

    authors: de Ville de Goyet J,Hausleithner V,Malaise J,Reding R,Lerut J,Jamart J,Barker A,Otte JB

    更新日期:1994-05-15 00:00:00