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High glucose levels decrease proliferation of cultured human fetal cells from placenta.

Abstract：

:We used the placenta as a source of undifferentiated cells to study the effect high glucose levels can have on human fetal cell proliferation in vitro. Cells were subcultured in a modified minimum essential medium with 10% fetal bovine serum containing either 5.5 mmol/L (100 mg/dl) D-glucose (control), 11 mmol/L (200 mg/dl) D-glucose, or 22 mmol/L (400 mg/dl) D-glucose. Cells grown in mannitol-containing media were used as controls for osmolality. After 3 and 7 days' growth in different media, the labeling index was determined by autoradiographic analysis, and cell numbers were determined with a Coulter counter. The labeling indices for cells grown 3 days in 11 or 22 mmol/L D-glucose were 89% (p less than 0.002) and 84% (p less than 0.001), respectively, of control cells grown in 5.5 mmol/L D-glucose. After 7 days' growth, the labeling indices of cells grown in 11 or 22 mmol/L D-glucose were 84% (p less than 0.002) and 70% (p less than 0.001), respectively, of cells grown in 5.5 mmol/L D-glucose media. There was a significant decrease in the number of cells present at both 3 and 7 days in cultures grown in 22 mmol/L D-glucose compared with control. We conclude that a few day's exposure to high glucose levels can have an effect on proliferation of human placental cells in vitro. We suggest that a glucose effect on proliferation of other cells derived from the products of conception might be one mechanism contributing to abnormal development in some pregnancies of diabetic women.

journal_name

Am J Obstet Gynecol

journal_title

American journal of obstetrics and gynecology

authors

Nelson DM,Curran EM

doi

10.1016/0002-9378(89)90925-3

subject

Has Abstract

pub_date

1989-12-01 00:00:00

pages

1553-8

issue

6 Pt 1

eissn

0002-9378

issn

1097-6868

pii

0002-9378(89)90925-3

journal_volume

161

pub_type

杂志文章

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