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Immunoregulatory activity in supernatants from cultures of normal human trophoblast cells of the first trimester.

Abstract：

:Supernatants from isolated trophoblast cell cultures (trophoblastic fluid) derived from first-trimester human placentas were assessed for immunoregulatory activity. Trophoblastic fluid at different days of culture consistently inhibited the blast transformation of allogenic lymphocytes. This suppressor effect had no apparent correlation with biosynthesis of human chorionic gonadotropin by trophoblast cells, since this hormone was secreted into the culture fluid only for the initial 3 days. However, the culture fluids of such purified trophoblast cells contained an immunosuppressive factor, pregnancy-associated plasma protein A, which was measurable throughout the culture period of 8 days. The presence of pregnancy-associated plasma protein A in significant amounts in trophoblastic fluid collected at daily intervals indicated a continuous secretion ability of pregnancy-associated plasma protein A by trophoblast cells in culture parallel to the suppressive immunoregulatory effect of the fluid. Such immunosuppressive effect was absent in the culture fluids of control BeWo malignant trophoblast cells; the BeWo cell culture fluids had markedly reduced levels of pregnancy-associated plasma protein A. The culture supernatant of normal trophoblast cells of placentas from first-trimester pregnancy activated in vitro the generation of a population of suppressor lymphocytes. This effect is generally considered responsible for immunologic tolerance. Therefore demonstration of immunosuppressive effects and the presence of relatively high levels of pregnancy-associated plasma protein A in trophoblastic fluid indicate that such proteins secreted by the trophoblast cells may be important in the local immunoregulatory processes of the fetal allograft.

journal_name

Am J Obstet Gynecol

journal_title

American journal of obstetrics and gynecology

authors

Sanyal MK,Brami CJ,Bischof P,Simmons E,Barnea ER,Dwyer JM,Naftolin F

doi

10.1016/0002-9378(89)90540-1

subject

Has Abstract

pub_date

1989-08-01 00:00:00

pages

446-53

issue

eissn

0002-9378

issn

1097-6868

pii

0002-9378(89)90540-1

journal_volume

161

pub_type

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Immunoregulatory activity in supernatants from cultures of normal human trophoblast cells of the first trimester.