Abstract：
OBJECTIVE:Our objective was to identify novel genes that are expressed in temporally and spatially restricted patterns during mouse embryonic development and organogenesis. STUDY DESIGN:Two genetic trapping reporter constructs that lack transcriptional regulatory sequences were introduced independently into transcriptionally active gene loci by electroporation into mouse embryonic stem cells. Patterns of host gene-reporter construct expression were investigated in differentiated embryonic stem cells, embryoid bodies, and chimeric embryos at various stages of development. RESULTS:Three patterns of host gene-reporter construct expression were observed from the developmental analysis of four vector-integrated cell lines. Reporter expression patterns reflecting developmental regulation, constitutive activity, and developmental inactivation of the host genes were observed. CONCLUSIONS:Two vector-integrated gene loci from cell lines CCE-1C1 and D3-B44 have expression patterns not previously described by genetic trapping. Molecular characterization of these interrupted genes will shed light on their developmental function.
journal_name
Am J Obstet Gynecoljournal_title
American journal of obstetrics and gynecologyauthors
Johnson MD,Mahon KAdoi
10.1016/0002-9378(93)90643-wsubject
Has Abstractpub_date
1993-09-01 00:00:00pages
683-9issue
3eissn
0002-9378issn
1097-6868pii
0002-9378(93)90643-Wjournal_volume
169pub_type
杂志文章abstract：:The effect of estradiol infusion on uterine activity and peripheral levels of prostaglandin F and progesterone in pregnant women at term were investigated. 5 normal women at term and 4 women with pre-eclampsia at term were given estradiol-17 beta by infusion over 2-4 hours. Radioimmunoassay was used to measure perip...
journal_title：American journal of obstetrics and gynecology
pub_type： 杂志文章
doi：10.1016/0002-9378(73)90646-7
更新日期：1973-09-15 00:00:00
abstract：OBJECTIVE:Closure of the fetal ductus arteriosus, which is usually due to nonsteroidal antiinflammatory agents, may be detrimental. Therefore prenatal and postnatal clinical and echocardiographic findings in four human fetuses with spontaneous ductus arteriosus occlusion are reported. STUDY DESIGN:Echocardiographic an...
journal_title：American journal of obstetrics and gynecology
pub_type： 杂志文章
doi：10.1016/s0002-9378(96)70317-4
更新日期：1996-03-01 00:00:00
abstract：OBJECTIVE:The purposes of this study were to examine endothelial nitric oxide synthase expression in endothelial cells and to determine whether the inhibition of endothelial nitric oxide synthase could impair endothelial barrier function in preeclampsia. STUDY DESIGN:Messenger RNA and protein expression for endothelia...
journal_title：American journal of obstetrics and gynecology
pub_type： 杂志文章
doi：10.1016/j.ajog.2003.09.049
更新日期：2004-03-01 00:00:00
abstract：:The sinusoidal fetal heart rate pattern is a phenomenon of elusive pathophysiologic features and uncertain clinical significance. We present a case of sinusoidal fetal heart rate that occurred in association with fetal breathing. The degree and type of synchrony between the sinusoidal fetal heart rate and breathing mo...
journal_title：American journal of obstetrics and gynecology
pub_type： 杂志文章
doi：10.1016/s0002-9378(89)80060-2
更新日期：1989-03-01 00:00:00
abstract：:To clarify the possible immunologic functions of the endometrium before implantation, supernatants from explant cultures of human endometrial tissues were examined for effects on mixed lymphocyte reaction and phytohemagglutinin M-stimulated cultures. Supernatants obtained from both proliferative and secretory phase en...
journal_title：American journal of obstetrics and gynecology
pub_type： 杂志文章
doi：10.1016/s0002-9378(87)80095-9
更新日期：1987-10-01 00:00:00
abstract：BACKGROUND:Randomized controlled trials that have assessed the efficacy of cervical pessary to prevent preterm birth in asymptomatic high-risk women have reported conflicting results. OBJECTIVE:To evaluate the efficacy and safety of cervical pessary to prevent preterm birth and adverse perinatal outcomes in asymptomat...
journal_title：American journal of obstetrics and gynecology
pub_type： 杂志文章,meta分析
doi：10.1016/j.ajog.2019.12.266
更新日期：2020-07-01 00:00:00
abstract：OBJECTIVE:Our purpose was to determine differential effects of natural and synthetic glucocorticoids on cytochrome 17alpha-hydroxylase and cytochrome P-450 side-chain cleavage messenger ribonucleic acid in the sheep placenta and to determine whether feed-forward effects during labor are involved in further inducing pla...
journal_title：American journal of obstetrics and gynecology
pub_type： 杂志文章
doi：10.1016/s0002-9378(99)70619-8
更新日期：1999-05-01 00:00:00
abstract：:The aim of this study was to observe the sequential effects of oxygenation, umbilical cord occlusion, and finally cooling on circulating catecholamines and nonshivering thermogenesis in fetal sheep. We studied five fetal sheep at 132 +/- 3 days' gestation. The fetuses were first ventilated with oxygen; PaO2 levels wer...
journal_title：American journal of obstetrics and gynecology
pub_type： 杂志文章
doi：10.1016/0002-9378(91)90655-b
更新日期：1991-01-01 00:00:00
abstract：OBJECTIVE:The purpose of this study was to analyze the trend in subspecialization among men graduating from obstetrics and gynecology residency programs. STUDY DESIGN:Results of the Survey of Residents Completing Training in New York State were analyzed for 1998 to 2003. The proportions of men and women graduating fro...
journal_title：American journal of obstetrics and gynecology
pub_type： 杂志文章
doi：10.1016/j.ajog.2006.07.043
更新日期：2006-11-01 00:00:00
abstract：:Epidermal growth factor promotes growth in many cell types. The role of epidermal growth factor during gestation and fetal development is unknown. This study investigates the presence and binding characteristics of epidermal growth factor receptors in placentas throughout gestation. Cell membrane preparations were obt...
journal_title：American journal of obstetrics and gynecology
pub_type： 杂志文章
doi：10.1016/0002-9378(83)90249-1
更新日期：1983-12-15 00:00:00
abstract：:An hCG-like material has been extracted from human sperm. These experiments were designed to characterize this material. Sperms of 10 volunteers were separated from seminal fluid, washed in PBS three times, and resuspended in 0.5 ml of the same buffer. Samples were pooled; cells were disrupted by sonication and extrac...
journal_title：American journal of obstetrics and gynecology
pub_type： 杂志文章
doi：10.1016/0002-9378(79)90733-6
更新日期：1979-12-15 00:00:00
abstract：:In Finland an organized mass screening program covers the total female population. Based on the material (429,832) from a nationwide mass screening registry it was estimated that the female population not subjected to electrocoagulation of the uterine cervix (362,511) had a sixfold risk of frankly invasive carcinoma, ...
journal_title：American journal of obstetrics and gynecology
pub_type： 杂志文章
doi：10.1016/0002-9378(78)90115-1
更新日期：1978-07-01 00:00:00
abstract：OBJECTIVE:Our purpose was to determine whether detection of insulin-like growth factor-binding protein-1 in vaginal secretions could be used in the diagnosis of fetal membrane rupture. STUDY DESIGN:Consenting patients (n = 105) with complaints suspicious of membrane rupture between 24 and 42 weeks of gestation who had...
journal_title：American journal of obstetrics and gynecology
pub_type： 杂志文章
doi：10.1016/0002-9378(94)90461-8
更新日期：1994-07-01 00:00:00
abstract：OBJECTIVE:The aim of this study was to test the hypothesis that obstetricians' choice of delivery method is influenced by their risk attitude and perceived risk of complaints and malpractice litigation. STUDY DESIGN:The choice of delivery method in ambiguous cases was studied in a nationwide survey of Norwegian obstet...
journal_title：American journal of obstetrics and gynecology
pub_type： 杂志文章
doi：10.1016/j.ajog.2008.07.021
更新日期：2009-01-01 00:00:00
abstract：OBJECTIVES:We investigated whether maternal plasma levels of the placental hormone corticotropin-releasing hormone are elevated in pregnancies complicated by preterm labor. STUDY DESIGN:Mean maternal corticotropin-releasing hormone levels were studied in women who met specific criteria for preterm labor and in women w...
journal_title：American journal of obstetrics and gynecology
pub_type： 杂志文章
doi：10.1016/s0002-9378(11)90606-1
更新日期：1992-04-01 00:00:00
abstract：OBJECTIVE:The objective of this study was to assess the reliability and validity of an objective structured assessment of technical skills (OSATS) for repair of fourth-degree obstetric lacerations on a surgical model. STUDY DESIGN:Three blinded judges reviewed recordings of 20 junior and 20 senior obstetrics-gynecolog...
journal_title：American journal of obstetrics and gynecology
pub_type： 杂志文章
doi：10.1016/j.ajog.2008.07.054
更新日期：2008-12-01 00:00:00
abstract：OBJECTIVE:Our purpose was to determine the efficacy of transvaginal electrical stimulation in treating genuine stress incontinence. STUDY DESIGN:This was a multicenter, prospective, randomized, double-blind, placebo-controlled 15-week trial comparing the use of an active pelvic floor stimulator with a sham device. Thi...
journal_title：American journal of obstetrics and gynecology
pub_type： 临床试验,杂志文章,多中心研究,随机对照试验
doi：10.1016/0002-9378(95)90172-8
更新日期：1995-07-01 00:00:00
abstract：OBJECTIVE:We estimated the risk of complications at birth of extremely large babies (≥5000 g). STUDY DESIGN:This was a cohort study including all births of extremely large babies in 1996 through 2005 and comparison cohort with normal birthweight (1:2) identified in the national birth registration. RESULTS:There were ...
journal_title：American journal of obstetrics and gynecology
pub_type： 杂志文章
doi：10.1016/j.ajog.2010.12.036
更新日期：2011-05-01 00:00:00
abstract：OBJECTIVE:Our purpose was to assess the effects of labor and antigestagens on production of interleukin 8 by the term human placenta and to localize interleukin 8 in first- and third- trimester placentas. STUDY DESIGN:The study was conducted by the Department of Obstetrics and Gynaecology of the University of Edinburg...
journal_title：American journal of obstetrics and gynecology
pub_type： 杂志文章
doi：10.1016/s0002-9378(98)70275-3
更新日期：1998-07-01 00:00:00
abstract：:The highest percentages of unintended pregnancies and the largest increases in births reported in the United States in recent years were in adolescents and in women more than 35 years of age. Increasing numbers of these women will require contraception to avoid unintended pregnancy. In adolescents the combined oral co...
journal_title：American journal of obstetrics and gynecology
pub_type： 杂志文章,评审
doi：10.1016/s0002-9378(12)90947-3
更新日期：1993-06-01 00:00:00
abstract：OBJECTIVE:Our purpose was to determine whether maternal administration of prophylactic ampicillin or erythromycin after preterm amnion rupture is associated with maternal or neonatal benefits. STUDY DESIGN:Women with singleton pregnancies between 24 and 33 weeks 6 days of gestation were eligible if they had no immedia...
journal_title：American journal of obstetrics and gynecology
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1016/0002-9378(93)90038-k
更新日期：1993-10-01 00:00:00
abstract：:Microarrays can be constructed with dozens to millions of probes on their surface to allow high-throughput analyses of many biologic processes to be performed simultaneously on the same sample. Microarrays are now widely used for gene expression analysis, deoxyribonucleic acid resequencing, single-nucleotide polymorph...
journal_title：American journal of obstetrics and gynecology
pub_type： 杂志文章,评审
doi：10.1016/j.ajog.2005.12.014
更新日期：2006-08-01 00:00:00
abstract：:Samples of cord blood from 46 deliveries were collected between 2 and 8 mo after an epidemic of influenza caused by A/Port Chalmers/73 (H3H2) virus. Of 10 samples with HAI antibody titers against A/Port Chalmers of 1:16 or greater, one had 2-mercaptoethanol-sensitive IgM class antibodies against the virus. Lymphocyte ...
journal_title：American journal of obstetrics and gynecology
pub_type： 杂志文章
doi：10.1016/0002-9378(81)90609-8
更新日期：1981-10-15 00:00:00
abstract：:To evaluate the relationship between cigarette smoking and the occurrence of placenta previa, we used interview and medical record data to conduct a case-control analysis of 69 placenta previa cases and 12,351 controls. The unadjusted relative risk estimate of placenta previa for women reported to have "ever smoked" d...
journal_title：American journal of obstetrics and gynecology
pub_type： 杂志文章
doi：10.1016/0002-9378(91)90217-f
更新日期：1991-07-01 00:00:00
abstract：:Although traditionally the exclusive domain of other medical specialties, pulmonary artery catheterization may be of tremendous benefit to a variety of obstetric and gynecologic patients. Our experience with such invasive hemodynamic monitoring in 72 patients in an obstetrics and gynecology service is presented. In 86...
journal_title：American journal of obstetrics and gynecology
pub_type： 杂志文章
doi：10.1016/s0002-9378(85)80145-9
更新日期：1985-06-15 00:00:00
abstract：OBJECTIVE:The objective of the study was to identify prognostic and environmental factors associated with vulvar carcinoma in young women. STUDY DESIGN:This study was a review of patients younger than 45 years who were diagnosed with vulvar squamous cell carcinoma between 1994 and 2006. RESULTS:Fifty-six patients wer...
journal_title：American journal of obstetrics and gynecology
pub_type： 杂志文章
doi：10.1016/j.ajog.2009.01.014
更新日期：2009-06-01 00:00:00
abstract：OBJECTIVE:This study investigated the possible roles of interleukin 6 and soluble interleukin 6 receptor in the growth of endometrial and endometriotic cells. STUDY DESIGN:Endometrial and endometriotic stromal cells were collected from the uterus or from ovarian chocolate cysts. We examined the effects of interleukin ...
journal_title：American journal of obstetrics and gynecology
pub_type： 杂志文章
doi：10.1016/s0002-9378(99)70599-5
更新日期：1999-05-01 00:00:00
abstract：OBJECTIVE:The purpose of this analysis was to study the relationship between an increasing cesarean delivery rate and term neonatal seizures and peripartum deaths. STUDY DESIGN:This was a retrospective analysis of annually collated institutional data on cesarean delivery and perinatal outcome. RESULTS:Of 77,350 women...
journal_title：American journal of obstetrics and gynecology
pub_type： 杂志文章
doi：10.1016/j.ajog.2004.06.102
更新日期：2005-01-01 00:00:00
abstract：OBJECTIVE:The purpose of this study was to summarize recent evidence regarding infection-associated preterm birth and to make appropriate recommendations. Antepartum treatment of lower genital tract infection or bacterial colonization has been found to reduce the incidence of preterm birth in the case of asymptomatic b...
journal_title：American journal of obstetrics and gynecology
pub_type： 杂志文章
doi：10.1016/j.ajog.2004.03.014
更新日期：2004-06-01 00:00:00
abstract：:Different perspectives toward symptoms of urogenital aging exist among postmenopausal women, influenced by social and cultural factors, but one constant is that women rarely feel able to discuss urogenital problems freely--with their physicians, husbands, or friends. European women >55 years old were brought up to pri...
journal_title：American journal of obstetrics and gynecology
pub_type： 杂志文章,评审
doi：10.1016/s0002-9378(98)70556-3
更新日期：1998-05-01 00:00:00
