The storage time of monoiodinated gastrin affects the biological activity and binding to rat fundic plasma membranes similarly, whereas the immunoreactivity is less affected.

Abstract:

:Monoiodinated gastrin was produced by a gentle Iodo-gen method, followed by gel and ion-exchange chromatography. During storage this tracer has previously been found to retain its immunological activity, whereas its specific binding to rat gastric fundic plasma membranes decreased rapidly. To examine whether the loss of specific binding could be reflected in loss of biological activity, we examined the tracer at regular intervals for immunoreactivity by binding to an antibody directed against the C-terminal bioactive site of the gastrin molecule; for biological activity in the totally isolated, vascularly perfused rat stomach concomitantly stimulated with a phosphodiesterase inhibitor; for specific binding ('receptor binding') to a rat gastric fundic plasma membrane fraction; and for fragmentation and intramolecular changes by fast protein liquid chromatography (FPLC). Biological activity and 'specific' binding showed a parallel decrease to zero during 4 weeks of storage, whereas the immunoreactivity was retained much longer. There was no apparent fragmentation of the gastrin molecule during the 1st month after preparation as evaluated by FPLC. This study accordingly shows that both biological activity and specific binding to a gastric fundic plasma membrane fraction of 125I-gastrin are lost before detectable loss in immunoreactivity and before FPLC-detectable fragmentation of the molecule. Thus, during the early storage period subtle changes in the 125I-gastrin molecule must take place. Since the specific binding is so closely correlated to biological activity, it suggests that the binding actually represents true receptor binding.

journal_name

Scand J Gastroenterol

authors

Kleveland PM,Waldum HL

doi

10.3109/00365528708991480

subject

Has Abstract

pub_date

1987-05-01 00:00:00

pages

390-6

issue

4

eissn

0036-5521

issn

1502-7708

journal_volume

22

pub_type

杂志文章
  • Macroamylasaemia, IgA hypergammaglobulinaemia and autoimmunity in a patient with Down syndrome and coeliac disease.

    abstract::The association between macroamylasaemia and coeliac disease in Down syndrome with multiple autoimmune abnormalities has never been reported. A 40-year-old woman with a 15-year history of immunoglobulin A and immunoglobulin M hypergammaglobulinaemia, chronic diarrhoea, persistent mild aspartate aminotransferase (AST) ...

    journal_title:Scandinavian journal of gastroenterology

    pub_type: 杂志文章

    doi:10.1080/00365520310000933

    authors: Bermejo JF,Carbone J,Rodriguez JJ,Macias R,Rodriguez M,Gil J,Marin MA,Torres F,Fernandez-Cruz E

    更新日期:2003-04-01 00:00:00

  • Beta-adrenoceptor blockade in the treatment of postoperative adynamic ileus.

    abstract::Abdominal trauma, such as surgery and peritonitis, leads to inhibition of intestinal motility, partly mediated by alpha- and beta-adrenoceptors. To investigate the effect of nonselective beta-blockade on adynamic ileus, propranolol was compared with placebo in the postoperative course after elective colonic surgery in...

    journal_title:Scandinavian journal of gastroenterology

    pub_type: 临床试验,杂志文章,随机对照试验

    doi:10.3109/00365528708991872

    authors: Hallerbäck B,Carlsen E,Carlsson K,Enkvist C,Glise H,Haffner J,Innes R,Kirnö K

    更新日期:1987-03-01 00:00:00

  • Effect of erythromycin administration on upper gastrointestinal motility in scleroderma patients.

    abstract:BACKGROUND:Gastrointestinal involvement is frequent in patients with scleroderma. Erythromycin, a macrolide antibiotic, has been shown to accelerate gastric emptying in normal subjects and diabetic patients. The present study investigated the effects of acute erythromycin administration on gastric and gallbladder motil...

    journal_title:Scandinavian journal of gastroenterology

    pub_type: 临床试验,杂志文章

    doi:10.3109/00365529409092515

    authors: Fiorucci S,Distrutti E,Bassotti G,Gerli R,Chiucchiù S,Betti C,Santucci L,Morelli A

    更新日期:1994-09-01 00:00:00

  • The use of a short-lived radionuclide in the investigation of acute bleeding in the upper gastrointestinal tract.

    abstract::A method is described for the detection and monitoring of bleeding in the upper gastrointestinal tract. A short-lived radionuclide (113In-m) is used. Acute bleeding in the upper gastrointestinal tract in patients without signs of shock and preshock usually stopped within a short time after onset. ...

    journal_title:Scandinavian journal of gastroenterology

    pub_type: 杂志文章

    doi:

    authors: Pettersson C,Forssman O,Hollender A,Mulder JL

    更新日期:1975-01-01 00:00:00

  • Entero-oxyntin: a stimulant of gastric acid secretion extracted from porcine intestine.

    abstract::A peptide that further augments gastric acid secretion in cats, maximally stimulated by pentagastrin, was identified in porcine upper small intestine. Since the peptide stimulated basal and maximally pentagastrin-stimulated acid secretion to the same extent, it appears to act on a receptor different from the gastrin r...

    journal_title:Scandinavian journal of gastroenterology

    pub_type: 杂志文章

    doi:10.3109/00365528009181425

    authors: Vagne M,Mutt V

    更新日期:1980-01-01 00:00:00

  • Degradation of amino acids to short-chain fatty acids in humans. An in vitro study.

    abstract::Short-chain fatty acids (SCFA) originate mainly in the colon through bacterial fermentation of polysaccharides. To test the hypothesis that SCFA may originate from polypeptides as well, the production of these acids from albumin and specific amino acids was examined in a faecal incubation system. Albumin was converted...

    journal_title:Scandinavian journal of gastroenterology

    pub_type: 杂志文章

    doi:10.3109/00365528809103964

    authors: Rasmussen HS,Holtug K,Mortensen PB

    更新日期:1988-03-01 00:00:00

  • The effect of vasoactive intestinal peptide, secretin, and glucagon on human duodenal bicarbonate secretion.

    abstract::Duodenal luminal acidification increases duodenal mucosal bicarbonate production and also releases both secretin and vasoactive intestinal peptide (VIP). The effect of these two structurally similar peptides on human duodenal bicarbonate production has not been examined in humans. Our purpose was therefore to assess t...

    journal_title:Scandinavian journal of gastroenterology

    pub_type: 杂志文章

    doi:10.3109/00365528909093030

    authors: Wolosin JD,Thomas FJ,Hogan DL,Koss MA,O'Dorisio TM,Isenberg JI

    更新日期:1989-03-01 00:00:00

  • Chronic perineal fistula as a late failure manifestation of pseudomyxoma peritonei syndrome.

    abstract::Pseudomyxoma peritonei syndrome (PMP) is a rare but fascinating entity in surgical oncology. It consists of the accumulation of mucus in the peritoneal cavity, sometimes in huge amounts, in most of the cases after rupture of an appendiceal tumor. The diagnosis and management of PMP has changed significantly in the pas...

    journal_title:Scandinavian journal of gastroenterology

    pub_type: 杂志文章

    doi:10.1080/00365520410003623

    authors: Sebbag G,Sugarbaker PH,Walfisch S,Levy I

    更新日期:2004-11-01 00:00:00

  • Interferon alfa-2b alone or combined with recombinant granulocyte-macrophage colony-stimulating factor as treatment of chronic hepatitis C.

    abstract:BACKGROUND:To compare the effect of combination therapy with interferon-alpha (INF-alpha) and granulocyte-macrophage colony-stimulating factor (GM-CSF) to monotherapy with INF-alpha in patients with chronic hepatitis C infection. METHODS:Forty-five consecutive patients with chronic hepatitis C, all presenting with ele...

    journal_title:Scandinavian journal of gastroenterology

    pub_type: 临床试验,杂志文章,多中心研究,随机对照试验

    doi:

    authors: Grønbaek K,Krarup HB,Ring-Larsen H,Schaffalitzky de Muckadell O,Møller A,Schlichting P,Vyberg M

    更新日期:2002-07-01 00:00:00

  • High frequency of parasitic and viral stool pathogens in patients with active ulcerative colitis: report from a tropical country.

    abstract:OBJECTIVE:Diarrhoeal relapses in patients with ulcerative colitis (UC) may be associated with enteric infections and its diagnosis may lessen avoidable exposure to corticosteroids and/or immunosuppressants. The purpose of this study was to assess the frequency of stool pathogens (parasitic and viral) in patients with a...

    journal_title:Scandinavian journal of gastroenterology

    pub_type: 杂志文章

    doi:10.1080/00365520802556809

    authors: Banerjee D,Deb R,Dar L,Mirdha BR,Pati SK,Thareja S,Falodia S,Ahuja V

    更新日期:2009-01-01 00:00:00

  • Endoscopic papillectomy and KRAS expression in the treatment of adenoma in the major duodenal papilla.

    abstract:OBJECTIVE:The use of endoscopic papillectomy for resecting adenomas in the major duodenal papilla is increasing. This study focuses on the following three issues: Can endoscopic papillectomy be performed as a safe diagnostic and/or therapeutic procedure in biopsy-verified or suspected ampullary adenoma? Does expression...

    journal_title:Scandinavian journal of gastroenterology

    pub_type: 杂志文章

    doi:10.3109/00365521.2015.1046912

    authors: Haraldsson E,Swahn F,Verbeke C,Mattsson JS,Enochsson L,Ung KA,Lundell L,Heuchel R,Löhr JM,Arnelo U

    更新日期:2015-01-01 00:00:00

  • Transjugular intrahepatic portosystemic shunt with the Strecker stent for control of refractory acute and chronic variceal bleeding.

    abstract:BACKGROUND:We wanted to assess prospectively the safety, efficacy, and applicability of transjugular intrahepatic portosystemic shunt (TIPS) with the Strecker stent, focusing mainly on clinical and hemodynamic medium- and long-term follow-up. METHODS:Fifty-two patients reached an indication to perform a TIPS, in an em...

    journal_title:Scandinavian journal of gastroenterology

    pub_type: 杂志文章

    doi:10.3109/00365529609004880

    authors: Canizares RB,Rodriguez-Laiz JM,Martin MC,Belda AE,Alonso FC,Roldan FP,Castro LS,Arregui EC,Ricote GC

    更新日期:1996-03-01 00:00:00

  • Crohn's disease. Recurrence after surgical treatment.

    abstract::A series of 76 patients who have undergone surgical treatment for Crohn's disease is presented. In 14 of the patients surgery was performed for recurrence after previous resection. In total, 91 intestinal resections and one bypass--operation were carried out. Postoperative mortality comprised 4 patients (5.3%), and th...

    journal_title:Scandinavian journal of gastroenterology

    pub_type: 杂志文章

    doi:10.3109/00365527709181336

    authors: Nygaard K,Fausa O

    更新日期:1977-01-01 00:00:00

  • Efficacy of preemptive endoscopic submucosal dissection and surgery for synchronous colorectal neoplasms.

    abstract:BACKGROUND AND AIM:If patients with surgically resectable colorectal cancershave synchronous colorectal neoplasms that are difficult to remove by endoscopic mucosal resection, preemptive endoscopic submucosal dissection (ESD) can avoid excessive colorectal resection. The aim of this study was to evaluate the efficacy o...

    journal_title:Scandinavian journal of gastroenterology

    pub_type: 杂志文章

    doi:10.1080/00365521.2020.1792542

    authors: Yabuuchi Y,Imai K,Hotta K,Ito S,Kishida Y,Manabe S,Yamaoka Y,Hino H,Kagawa H,Shiomi A,Ono H

    更新日期:2020-08-01 00:00:00

  • A non-invasive fibrosis score predicts treatment outcome in chronic hepatitis C virus infection.

    abstract:OBJECTIVE:The results of a previous study suggest that an index calculated according to the formula (normalized ASAT x PK-INR) x 100/thrombocyte count (x 10(9)/L; GUCI) may reflect liver fibrosis in patients with chronic hepatitis C virus (HCV) infection. The aims of the present study were (i) to validate the associati...

    journal_title:Scandinavian journal of gastroenterology

    pub_type: 杂志文章

    doi:10.1080/00365520701514461

    authors: Westin J,Ydreborg M,Islam S,Alsiö A,Dhillon AP,Pawlotsky JM,Zeuzem S,Schalm SW,Ferrari C,Neumann AU,Hellstrand K,Lagging M,DITTO-HCV Study Group.

    更新日期:2008-01-01 00:00:00

  • Positive serum antigliadin antibodies without celiac disease in the elderly population: does it matter?

    abstract:OBJECTIVE:Antigliadin antibodies (AGA) show good sensitivity but low specificity for celiac disease and can also be found in healthy individuals. However, data suggest that AGA positivity might be related to distinct disease entities such as allergy and gluten ataxia. Our aim here is to explore the clinical relevance o...

    journal_title:Scandinavian journal of gastroenterology

    pub_type: 杂志文章

    doi:10.3109/00365521.2010.496491

    authors: Ruuskanen A,Kaukinen K,Collin P,Huhtala H,Valve R,Mäki M,Luostarinen L

    更新日期:2010-10-01 00:00:00

  • Serum infliximab concentrations in pediatric inflammatory bowel disease.

    abstract:OBJECTIVE:To study serum infliximab (s-IFX) levels in pediatric patients with inflammatory bowel disease (IBD). SUBJECTS AND METHODS:s-IFX trough levels were measured in a total of 133 blood samples of 37 pediatric IBD patients (Crohn's disease, 23): 48 during the induction phase (weeks 2 and 6) and 85 during maintena...

    journal_title:Scandinavian journal of gastroenterology

    pub_type: 杂志文章

    doi:10.3109/00365521.2012.741619

    authors: Hämäläinen A,Sipponen T,Kolho KL

    更新日期:2013-01-01 00:00:00

  • Double-blind randomized trial of bismuth subsalicylate and clindamycin for treatment of Helicobacter pylori infection.

    abstract::We evaluated clindamycin and bismuth subsalicylate (Pepto-Bismol) for treatment of Helicobacter pylori infection. Patients with culture or histology positive for H. pylori were randomized to receive two tablets of bismuth subsalicylate four times daily for 4 weeks or bismuth combined with 2 weeks of 300 mg clindamycin...

    journal_title:Scandinavian journal of gastroenterology

    pub_type: 临床试验,杂志文章,随机对照试验

    doi:10.3109/00365529208999958

    authors: Westblom TU,Madan E,Subik MA,Duriex DE,Midkiff BR

    更新日期:1992-01-01 00:00:00

  • Systematic expression profiling of the gastric H+/K+ ATPase in human tissue.

    abstract:OBJECTIVE:The potassium-competitive acid blockers (P-CABs), comprise a new, innovative group of competitive and reversible inhibitors of the gastric H+/K+ ATPase. Our aim was to identify sites of expression of the H+/K+ ATPase that are potential targets of these compounds by examining the expression profile of the gast...

    journal_title:Scandinavian journal of gastroenterology

    pub_type: 杂志文章

    doi:10.1080/00365520701405579

    authors: Herrmann M,Selige J,Raffael S,Sachs G,Brambilla A,Klein T

    更新日期:2007-11-01 00:00:00

  • Ascites as the first manifestation of polyarteritis nodosa.

    abstract::Polyarteritis nodosa (PAN) is a systemic necrotizing vasculitis that affects numerous organs, particularly the digestive tract (33-52% of cases). However, isolated ascites has rarely been described in patients with PAN. We observed a patient who had developed ascites revealing PAN, and because of suspected malignancy ...

    journal_title:Scandinavian journal of gastroenterology

    pub_type: 杂志文章

    doi:10.1080/00365520600575670

    authors: Hervé F,Héron F,Levesque H,Marie I

    更新日期:2006-04-01 00:00:00

  • Chronic non-A, non-B hepatitis. A long-term follow-up study in 49 patients.

    abstract::Forty-nine patients with biopsy-verified chronic non-A, non-B hepatitis (NANBH) of both percutaneously transmitted and sporadic types were followed up for up to 20 years (mean, 62 months +/- 44 months). Drug addicts were not included. Twenty-four patients had chronic persistent hepatitis (CPH), and 25 had chronic acti...

    journal_title:Scandinavian journal of gastroenterology

    pub_type: 杂志文章

    doi:10.3109/00365528708991968

    authors: Wejstål R,Lindberg J,Lundin P,Norkrans G

    更新日期:1987-11-01 00:00:00

  • A novel one-hour 13C-sorbitol breath test versus the H2-sorbitol breath test for assessment of coeliac disease.

    abstract:OBJECTIVE:The H(2)-sorbitol breath test (H(2)-SBT) has previously been suggested as a screening tool for coeliac disease. We developed an alternative (13)C-sorbitol breath test ((13)C-SBT). The aim of the study was to compare the diagnostic properties of the H(2)-SBT and the (13)C-SBT in a clinical setting. MATERIAL A...

    journal_title:Scandinavian journal of gastroenterology

    pub_type: 杂志文章

    doi:10.1080/00365520902846233

    authors: Tveito K,Hetta AK,Askedal M,Brunborg C,Sandvik L,Løberg EM,Skar V

    更新日期:2009-01-01 00:00:00

  • Significance of cell-surface expression of matrix metalloproteinases and their inhibitors on gastric epithelium and infiltrating mucosal lymphocytes in progression of Helicobacter pylori-associated gastritis.

    abstract:BACKGROUND:Matrix metalloproteinases (MMPs) and their inhibitors (TIMPs) have recently been shown to be important in tissue breakdown and remodeling in gut with inflammatory bowel disease. The role of MMPs and TIMPs remains largely unexplored in Helicobacter pylori-associated gastritis (HAG). The aim of this study was ...

    journal_title:Scandinavian journal of gastroenterology

    pub_type: 杂志文章

    doi:10.1080/00365520410003245

    authors: Koyama S

    更新日期:2004-11-01 00:00:00

  • Calcium malabsorption and absence of bone decalcination following intestinal shunt operation for obesity. A comparison of two types of operation.

    abstract::Calcium absorption was studied in 23 patients before and one year after intestinal shunt operation for obesity. It decreased from 23 to 12% (p less than 0.02) after surgery, when the jejunum/ileum ratio was 36cm/12cm [type I], and only from 19 to 16% (p greater than 0.05), when the ratio was 12cm/36cm [type III]. It i...

    journal_title:Scandinavian journal of gastroenterology

    pub_type: 杂志文章

    doi:10.3109/00365527809179810

    authors: Danö P,Christiansen C

    更新日期:1978-01-01 00:00:00

  • Gastric emptying: comparison of scintigraphic, polyethylene glycol dilution, and paracetamol tracer assessment techniques.

    abstract:BACKGROUND:The method of choice for studying gastric emptying is dependent on several factors. The aim of the present study was to assess the concordance between solid gastric emptying, using a scintigraphic technique as gold standard, and gastric emptying as measured with parcetamol tracer and polyethylene glycol (PEG...

    journal_title:Scandinavian journal of gastroenterology

    pub_type: 杂志文章

    doi:10.1080/003655200750023930

    authors: Näslund E,Bogefors J,Grybäck P,Jacobsson H,Hellström PM

    更新日期:2000-04-01 00:00:00

  • Protective effects of N-acetyl-L-cysteine and platelet activating factor inhibition are not linked to intercellular adhesion molecule-1 expression after intestinal ischemia and reperfusion injury in rats.

    abstract:BACKGROUND:Intestinal ischemia and reperfusion (I/R) injury may result in development of the systemic inflammatory response syndrome (SIRS). The interactions between activated leukocytes and endothelial cells, mediated by adhesion molecules, seem to be pivotal in these conditions, leading as they do to extravasation of...

    journal_title:Scandinavian journal of gastroenterology

    pub_type: 杂志文章

    doi:10.1080/00365520310002201

    authors: Olanders K,Börjesson A,Sun ZW,Andersson R

    更新日期:2003-06-01 00:00:00

  • Evaluation of preoperative prognostic factors in patients with resectable pancreatic ductal adenocarcinoma.

    abstract::Objective: Upfront surgery is the standard treatment for resectable pancreatic ductal adenocarcinomas (R-PDACs); however, these tumors often recur. We investigated the factors governing recurrence and prognosis in patients with R-PDAC. Methods: We analyzed 359 patients who underwent upfront surgery for R-PDAC between ...

    journal_title:Scandinavian journal of gastroenterology

    pub_type: 杂志文章

    doi:10.1080/00365521.2019.1624816

    authors: Izumo W,Higuchi R,Furukawa T,Yazawa T,Uemura S,Shiihara M,Yamamoto M

    更新日期:2019-06-01 00:00:00

  • Does water-perfused catheter overdiagnose anismus compared to balloon probe?

    abstract:BACKGROUND:The purpose of this study was to compare the manometric assessment of straining effort as if to defecate and rectoanal inhibitory reflex obtained with a rectosphincteric balloon probe and with a water-perfused catheter in the same subject. METHODS:Twelve healthy volunteers underwent two manometric assessmen...

    journal_title:Scandinavian journal of gastroenterology

    pub_type: 临床试验,杂志文章,随机对照试验

    doi:10.1080/003655202762671288

    authors: Savoye G,Leroi AM,Bertot-Sassigneux P,Touchais JY,Devroede G,Denis P

    更新日期:2002-12-01 00:00:00

  • Is gluten challenge necessary for the diagnosis of coeliac disease in young children?

    abstract::Sixty-seven children under 2 years of age presenting with a classic clinical picture of coeliac disease with a severe small-intestinal mucosal lesion were investigated. All improved clinically and histologically on a gluten-free diet. During gluten challenge the mucosal damage recurred in 64 (95.5%) children, thus ful...

    journal_title:Scandinavian journal of gastroenterology

    pub_type: 杂志文章

    doi:10.3109/00365529008997618

    authors: Danielsson L,Stenhammar L,Aström E

    更新日期:1990-09-01 00:00:00

  • Laparoscopic cholecystectomy: bile duct and vascular injuries: management and outcome.

    abstract:BACKGROUND:This is a retrospective study of 32 consecutive patients referred in the period 1992-2000 for management of serious bile duct injuries caused by elective laparoscopic cholecystectomy. METHODS:The patients were referred on median 29 days (0 days to 34 months). Only 7 patients were referred immediately after ...

    journal_title:Scandinavian journal of gastroenterology

    pub_type: 杂志文章

    doi:10.1080/003655202317316123

    authors: Mathisen O,Søreide O,Bergan A

    更新日期:2002-04-01 00:00:00