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A proximity based general method for identification of ligand and receptor interactions in living cells.

Abstract：

:Autocrine based selections from intracellular combinatorial antibody and peptide libraries have proven to be a powerful method for selection of agonists and identification of new therapeutic targets. However, success requires a case-by-case construction of a robust selection system which is a process that can be time consuming and expensive. Here we report a general system that takes advantage of the chemical rate acceleration caused by approximation of a membrane tethered ligand and its receptor. The system uses an artificial signal transduction and is, thus, agnostic to the endogenous signal transduction of the receptor-ligand system. This method allows analysis of receptor-ligand interactions and selection of molecules from large libraries that interact with receptors when they are in their natural milieu.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Zhang H,Xie J,Lerner RA

doi

10.1016/j.bbrc.2014.10.085

subject

Has Abstract

pub_date

2014-11-07 00:00:00

pages

251-5

issue

1

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(14)01894-4

journal_volume

454

pub_type

杂志文章

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