Abstract:
:In vitro (1)H MRS of human bile has shown potential in the diagnosis of various hepatopancreatobiliary (HPB) diseases. Previously, in vivo (1)H MRS of human bile in gallbladder using a 1.5 T scanner demonstrated the possibility of quantification of choline-containing phospholipids (chol-PLs). However, other lipid components such as bile acids play an important role in the pathophysiology of the HPB system. We have employed a higher magnetic field strength (3 T), and a custom-built receive array coil, to improve the quality of in vivo (1)H MRS of human bile in the gallbladder. We obtained significant improvement in the quality of 1D spectra (17 healthy volunteers) using a respiratory-gated PRESS sequence with well distinguished signals for total bile acids (TBAs) plus cholesterol resonating at 0.66 ppm, taurine-conjugated bile acids (TCBAs) at 3.08 ppm, chol-PLs at 3.22 ppm, glycine-conjugated bile acids (GCBAs) at 3.74 ppm, and the amide proton (-NH) arising from GCBAs and TCBAs in the region 7.76-8.05 ppm. The peak areas of these signals were measured by deconvolution, and subsequently the molar concentrations of metabolites were estimated with good accuracy, except for that of TBAs plus cholesterol. The concentration of TBAs plus cholesterol was overestimated in some cases, which could be due to lipid contamination. In addition, we report the first 2D L-COSY spectra of human gallbladder bile in vivo (obtained in 15 healthy volunteers). 2D L-COSY spectra will be helpful in differentiating various biliary chol-PLs in pathological conditions of the HPB system.
journal_name
NMR Biomedjournal_title
NMR in biomedicineauthors
Mohajeri S,Ijare OB,Bezabeh T,King SB,Thomas MA,Minuk G,Lipschitz J,Kirkpatrick I,Smith M,Smith ICdoi
10.1002/nbm.3173subject
Has Abstractpub_date
2014-10-01 00:00:00pages
1192-202issue
10eissn
0952-3480issn
1099-1492journal_volume
27pub_type
杂志文章abstract::Dynamic contrast-enhanced (DCE) magnetic resonance imaging (MRI) and proton (1H) magnetic resonance spectroscopy (MRS) provide structural and biochemical information, including vascular volume, vascular permeability and tissue metabolism. In this study, we performed analysis of the enhancement characteristic from DCE-...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.1511
更新日期:2010-10-01 00:00:00
abstract::The ability to assess the perfusion territories of major cerebral arteries can be a valuable asset to the diagnosis of a number of cerebrovascular diseases. Recently, several arterial spin labeling (ASL) techniques have been proposed for determining the cerebral perfusion territories of individual arteries by three di...
journal_title:NMR in biomedicine
pub_type: 杂志文章,评审
doi:10.1002/nbm.1177
更新日期:2007-11-01 00:00:00
abstract::Quantification of metabolites is a goal of many biomedical NMR studies. To obtain absolute measurements of metabolite concentrations is often both difficult and time-consuming. In this paper a method for determining metabolite concentrations directly is described and validated. It is applicable to studies of amphibian...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.1940050306
更新日期:1992-05-01 00:00:00
abstract::(31)P MRS provides a unique non-invasive window into myocardial energy homeostasis. Mouse models of cardiac disease are widely used in preclinical studies, but the application of (31)P MRS in the in vivo mouse heart has been limited. The small-sized, fast-beating mouse heart imposes challenges regarding localized sign...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.3371
更新日期:2015-10-01 00:00:00
abstract::Detailed (1)H and (13)C NMR spectroscopy of lipid extracts from 12 human intracranial tuberculomas and two control brain tissue samples was performed to assess the role of lipids in the disease process. One-dimensional and two-dimensional NMR techniques were used to resolve the mixture of lipid components and make res...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.1191
更新日期:2008-03-01 00:00:00
abstract::It is generally accepted that tumour microcirculation, blood flow, oxygen and nutrient supply, tissue pH distribution, and the bioenergetic status--factors which are usually closely linked and which define the so-called metabolic microenvironment--can markedly influence the therapeutic response of malignant tumours to...
journal_title:NMR in biomedicine
pub_type: 杂志文章,评审
doi:10.1002/nbm.1940050505
更新日期:1992-09-01 00:00:00
abstract::A novel polarizer based on the dissolution-dynamic nuclear polarization (DNP) method has been designed, built and tested. The polarizer differs from those previously described by being designed with sterile use intent and being compatible with clinical use. The main features are: (1) an integral, disposable fluid path...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.1682
更新日期:2011-10-01 00:00:00
abstract::The current state-of-the-art diagnosis method for deep tissue injury in muscle, a subcategory of pressure ulcers, is palpation. It is recognized that deep tissue injury is frequently preceded by altered biomechanical properties. A quantitative understanding of the changes in biomechanical properties preceding and duri...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.4087
更新日期:2019-06-01 00:00:00
abstract::Increased pulmonary lactate production is correlated with severity of lung injury and outcome in acute respiratory distress syndrome (ARDS) patients. This study was conducted to investigate the relative contributions of inflammation and hypoxia to the lung's metabolic shift to glycolysis in an experimental animal mode...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.4380
更新日期:2020-11-01 00:00:00
abstract::Perfusion is an important biomarker of tissue function and has been associated with tumor pathophysiology such as angiogenesis and hypoxia. Arterial spin labeling (ASL) MRI allows noninvasive and quantitative imaging of perfusion; however, the application in mouse xenograft tumor models has been challenging due to the...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.3330
更新日期:2015-08-01 00:00:00
abstract::This study tests the hypothesis that brief periods of ischemia result in an increase in myocardial lipids during early reperfusion. We conducted 1H NMR spectroscopy to serially measure myocardial lipids before and after regional ischemia. Localized 1H NMR spectra (spatial resolution of 1.25 mm) were obtained using a o...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.1940080206
更新日期:1995-04-01 00:00:00
abstract::High-resolution MR imaging (312 microns in plane resolution) and MR spectroscopy (0.36 cm3 nominal voxel) have been performed on human frontal cortex using a 3 in surface coil. Localized proton spectra have been obtained from contiguous 6 x 6 x 10 mm voxels using one-dimensional phase encoding, TR 2000 ms and TE 31 ms...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/(SICI)1099-1492(199610)9:7<297::AID-NBM433
更新日期:1996-10-01 00:00:00
abstract::Organs consist of several types of cells with specialized functions. This cellular localization of function is often referred to as compartmentation. Due to the intrinsic low sensitivity of MR methods it is generally not possible in vivo to obtain images or spectra of single cells. Instead the MRS signal is the sum of...
journal_title:NMR in biomedicine
pub_type: 杂志文章,评审
doi:10.1002/nbm.692
更新日期:2001-04-01 00:00:00
abstract::A short-echo spectroscopic imaging sequence extended with a frequency-selective multiple-quantum- coherence technique (Sel-MQC) is presented. The method enables acquisition of a complete water-suppressed proton spectrum with a short echo time and filtering of the J-coupling metabolite, lactate, from co-resonant lipids...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.1284
更新日期:2008-11-01 00:00:00
abstract::More than 50% of cancers fail to respond to any individual treatment and tumour follow-up after treatment plays a major role in routine therapy planning and pharmacological research. Today, MRS is the only technological approach providing non-invasive access to tumour biochemistry. Ten years ago, expectations were rai...
journal_title:NMR in biomedicine
pub_type: 杂志文章,评审
doi:10.1002/nbm.1940060602
更新日期:1993-11-01 00:00:00
abstract::One of the hallmarks of diffusion NMR and MRI is its ability to utilize restricted diffusion to probe compartments much smaller than the excited volume or the MRI voxel, respectively, and to extract microstructural information from them. Single-pulsed field gradient (s-PFG) MR methodologies have been employed with gre...
journal_title:NMR in biomedicine
pub_type: 杂志文章,评审
doi:10.1002/nbm.1550
更新日期:2010-08-01 00:00:00
abstract::The cerebral blood volume (CBV) is a crucial physiological indicator of tissue viability and vascular reactivity. Thus, noninvasive CBV mapping has been of great interest. For this, ultrasmall superparamagnetic iron oxide (USPIO) nanoparticles, including monocrystalline iron oxide nanoparticles, can be used as long-ha...
journal_title:NMR in biomedicine
pub_type: 杂志文章,评审
doi:10.1002/nbm.2885
更新日期:2013-08-01 00:00:00
abstract::Several obstacles usually confound a straightforward perfusion analysis using dynamic-susceptibility contrast-based magnetic resonance imaging (DSC-MRI). In this work, it became possible to eliminate some of these sources of error by combining a multiple gradient-echo technique with parallel imaging (PI): first, the l...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.1107
更新日期:2007-06-01 00:00:00
abstract::Exercise studies investigating the metabolic response of calf muscles using 31 P MRS are usually performed with a single knee angle. However, during natural movement, the distribution of workload between the main contributors to force, gastrocnemius and soleus is influenced by the knee angle. Hence, it is of interest ...
journal_title:NMR in biomedicine
pub_type: 临床试验,杂志文章
doi:10.1002/nbm.3905
更新日期:2018-06-01 00:00:00
abstract::This study investigated the association between the total choline (tCho) concentration and the clinical characteristics and biomarker status of breast cancer. Sixty-two patients with breast cancer, 1.5 cm or larger in size on MR images, were studied. The tCho concentration was correlated with the MRI features, contra...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.1595
更新日期:2011-04-01 00:00:00
abstract::Diffusion tensor MRI (DTI) is a method for the noninvasive assessment of cellular organization and integrity in vivo. In this study, in vivo DTI was performed to demonstrate its ability to reflect photoreceptor cell alignment in adult C57BL/6 wild-type mice. Age-matched retinal degeneration 1 (rd1) mice were employed ...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.1609
更新日期:2011-06-01 00:00:00
abstract::The aim of this study is to fabricate and test a novel flexible flat cable antenna (FFCA) for MRI of the knee at different flexion angles. The FFCA was made of a flat cable, a tuning/matching circuit and a signal transmission line. To test its feasibility and validity, in vitro and in vivo experiments were carried out...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.3264
更新日期:2015-04-01 00:00:00
abstract::Two FDA-approved agents, ferumoxides (Feridex), a suspension of superparamagnetic iron oxide (SPIO) nanoparticles and protamine sulfate, a drug used to reverse heparin anticoagulation, can be complexed and used to label cells magnetically ex vivo. Labeling stem cells with ferumoxides-protamine sulfate (FePro) complexe...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.991
更新日期:2005-12-01 00:00:00
abstract::In order to keep subscribers up-to-date with the latest developments in their field, John Wiley & Sons are providing a current awareness service in each issue of the journal. The bibliography contains newly published material in the field of NMR in biomedicine. Each bibliography is divided into 9 sections: 1 Books, Re...
journal_title:NMR in biomedicine
pub_type:
doi:10.1002/nbm.667
更新日期:2001-02-01 00:00:00
abstract::The increased magnetic susceptibility effects at higher magnetic fields increase the demands for shimming of the B0 field for in vivo MRI and MRS. Both static and dynamic techniques have been developed to compensate for susceptibility-induced field inhomogeneities. In this study, we investigate the impact of and need ...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.3096
更新日期:2014-06-01 00:00:00
abstract::Surface coil 13C nuclear magnetic resonance (NMR) spectroscopy was used to investigate the in vivo carbohydrate metabolism of rat C6 gliomas during and after infusion with [1-13C] glucose. In vivo 1H-decoupled 13C NMR spectra of the glioma following infusion with [1-13C]glucose revealed the direct production of [3-13C...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.1940010105
更新日期:1988-02-01 00:00:00
abstract::Patients with different types of mucopolysaccharidoses (MPS) lack specific lysosomal enzymes, which leads to tissue accumulation and urinary excretion of glycosaminoglycans (GAGs). Since little is known about the molecular composition of the excreted GAG fragments, we used two-dimensional [1H,13C]-correlation nuclear ...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.833
更新日期:2003-06-01 00:00:00
abstract::The aim of this study was to investigate the diffusion time dependence of signal-versus-b curves obtained from diffusion-weighted magnetic resonance imaging (DW-MRI) of sub-acute ischaemic lesions in stroke patients. In this case series study, 16 patients with sub-acute ischaemic stroke were examined with DW-MRI using...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.1376
更新日期:2009-07-01 00:00:00
abstract::Polycystic kidney disease (PKD) is a life-threatening disease that leads to a grotesque enlargement of the kidney and significant loss of function. Several imaging studies with MRI have demonstrated that cyst size in polycystic kidneys can determine disease severity and progression. In the present study, we found that...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.3281
更新日期:2015-05-01 00:00:00
abstract::T1ρ relaxation imaging is a quantitative imaging technique that has been used to assess cartilage integrity, liver fibrosis, tumors, cardiac infarction, and Alzheimer's disease. T1 , T2 , and T1ρ relaxation time constants have each demonstrated different degrees of sensitivity to several markers of fibrosis and inflam...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.4284
更新日期:2020-05-01 00:00:00