Abstract:
:We had shown that aromatic amino acid (phenylalanine, tyrosine, and tryptophan) supplementation prevented bone loss in an aging C57BL/6 mice model. In vivo results from the markers of bone breakdown suggested an inhibition of osteoclastic activity or differentiation. To assess osteoclastic differentiation, we examined the effects of aromatic amino acids on early /structural markers as vitronectin receptor, calcitonin receptor, and carbonic anhydrase II as well as, late/functional differentiation markers; cathepsin K and matrix metalloproteinase 9 (MMP-9). Our data demonstrate that the aromatic amino acids down-regulated early and late osteoclastic differentiation markers as measured by real time PCR. Our data also suggest a link between the vitronectin receptor and the secreted cathepsin K that both showed consistent effects to the aromatic amino acid treatment. However, the non-attachment related proteins, calcitonin receptor, and carbonic anhydrase II, demonstrated less consistent effects in response to treatment. Our data are consistent with aromatic amino acids down-regulating osteoclastic differentiation by suppressing remodeling gene expression thus contributing initially to the net increase in bone mass seen in vivo.
journal_name
Calcif Tissue Intjournal_title
Calcified tissue internationalauthors
Refaey ME,Zhong Q,Ding KH,Shi XM,Xu J,Bollag WB,Hill WD,Chutkan N,Robbins R,Nadeau H,Johnson M,Hamrick MW,Isales CMdoi
10.1007/s00223-014-9878-zsubject
Has Abstractpub_date
2014-08-01 00:00:00pages
174-82issue
2eissn
0171-967Xissn
1432-0827journal_volume
95pub_type
杂志文章abstract::ATP is as effective as inorganic pyrophosphate in retarding the rate of dissolution of calcium hydroxyapatite at pH 7, ADP is less effective, and AMP is without effect. The inhibition is due to the adsorption of ATP and ADP units on the crystal surface. ...
journal_title:Calcified tissue international
pub_type: 杂志文章
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journal_title:Calcified tissue international
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journal_title:Calcified tissue international
pub_type: 杂志文章
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journal_title:Calcified tissue international
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journal_title:Calcified tissue international
pub_type: 杂志文章
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journal_title:Calcified tissue international
pub_type: 杂志文章
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journal_title:Calcified tissue international
pub_type: 杂志文章,评审
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journal_title:Calcified tissue international
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journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/s00223-004-0228-4
更新日期:2004-12-01 00:00:00
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journal_title:Calcified tissue international
pub_type: 杂志文章,评审
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更新日期:1986-04-01 00:00:00
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journal_title:Calcified tissue international
pub_type: 杂志文章
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journal_title:Calcified tissue international
pub_type: 杂志文章,评审
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journal_title:Calcified tissue international
pub_type: 杂志文章
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journal_title:Calcified tissue international
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doi:10.1007/s00223-002-0021-1
更新日期:2004-05-01 00:00:00
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journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/s00223-016-0180-0
更新日期:2016-11-01 00:00:00
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journal_title:Calcified tissue international
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journal_title:Calcified tissue international
pub_type: 杂志文章
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更新日期:1991-11-01 00:00:00
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journal_title:Calcified tissue international
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journal_title:Calcified tissue international
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abstract::Toxicological studies have demonstrated that intermittent PTH1-34 treatment is associated with an increased incidence of osteosarcoma in Fischer 344 rats. Comet and micronucleus (MN) tests, standard methods to evaluate genotoxic potential of drugs, were used to detect DNA and chromosome breaks, respectively, after PTH...
journal_title:Calcified tissue international
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journal_title:Calcified tissue international
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journal_title:Calcified tissue international
pub_type: 杂志文章,随机对照试验
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更新日期:2007-05-01 00:00:00
abstract::To assess whether vertebral fractures are associated with osteopenia in chronic alcoholic patients, a transversal study was carried out in 76 chronic alcoholic males and 62 age-matched healthy males. Lumbar bone mineral density (BMD) by dual photon absorptiometry and spinal chest X-ray films were done in all patients....
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/BF00298430
更新日期:1995-08-01 00:00:00
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journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/s00223-017-0245-8
更新日期:2017-06-01 00:00:00