Abstract:
CONCLUSION:Human adipose-derived stem cells (ASCs), encapsulated in a fibrin-collagen hydrogel for the coating of an electrode array, produce sufficient amounts of neurotrophic factors and may be suitable for enhancing the bioelectric interface of cochlear implants (CIs). OBJECTIVES:To evaluate different hydrogel compositions loaded with ASCs with regard to delivery of neuroactive substances and mechanical suitability for the coating of a CI electrode array. METHODS:ASCs were cultivated in hydrogels consisting of collagen and fibrin in varying fractions (0:1, 1:1, 1:2, and 1:0). The cell proliferation and viability, as well as the production of brain-derived neurotrophic factor (BDNF), glial cell line-derived neurotrophic factor (GDNF), and laminin were determined. Two hydrogel compositions were used as a coating for CI electrode arrays and tested in a scala tympani model. RESULTS:Cell proliferation was best in collagen/fibrin hydrogel compositions (1:1 and 1:2) and increasing amounts of BDNF (up to 2.59 ng/ml) and laminin (up to 320 ng/ml) were detected. GDNF production was inconsistent and markedly lower. A sufficient coating of a CI electrode carrier in terms of stability and flexibility was achieved only with mixed compositions, although hydrogels formed bulky and uneven layers on the silicone surfaces.
journal_name
Acta Otolaryngoljournal_title
Acta oto-laryngologicaauthors
Schendzielorz P,Scherzed A,Rak K,Völker J,Hagen R,Mlynski R,Frölich K,Radeloff Adoi
10.3109/00016489.2013.878809subject
Has Abstractpub_date
2014-05-01 00:00:00pages
497-505issue
5eissn
0001-6489issn
1651-2251journal_volume
134pub_type
杂志文章abstract::Test-retest variability of values for directional asymmetry in primary and secondary horizontal optokinetic after-nystagmus (OKAN I and OKAN II, respectively) was studied in 16 apparently healthy subjects. OKAN was induced by 60 s of whole-field optokinetic stimulation at speeds of 60 degrees/s and 90 degrees/s in eit...
journal_title:Acta oto-laryngologica
pub_type: 杂志文章
doi:10.3109/00016489209100776
更新日期:1992-01-01 00:00:00
abstract::In previous studies, sinus secretions have been analysed concerning pO2, pCO2 and pH. In this study the energy metabolism in the maxillary sinus mucosa and secretion was studied in 33 New Zealand White rabbits. In freeze-dried samples, glucose and lactate were analysed by enzymatic assays and ATP by HPLC. In the purul...
journal_title:Acta oto-laryngologica
pub_type: 杂志文章
doi:10.3109/00016488809122271
更新日期:1988-11-01 00:00:00
abstract::The occurrence of horizontal jerks with larger amplitudes than on Earth was observed during vertical optokinetic nystagmus in astronauts tested throughout a 7-day spaceflight. During early exposure to microgravity, a horizontal spontaneous-like nystagmus was recorded in darkness following both vertical and horizontal ...
journal_title:Acta oto-laryngologica
pub_type: 杂志文章
doi:10.3109/00016489009107432
更新日期:1990-03-01 00:00:00
abstract::Background: The management of patients with tinnitus who receive middle ear surgery has not been established.Aims: The aim of this study is (1) how many patients who receive middle ear surgery have tinnitus and (2) how educational counselling and middle ear surgery is effective with consistent tinnitus.Subjects and me...
journal_title:Acta oto-laryngologica
pub_type: 杂志文章
doi:10.1080/00016489.2020.1713397
更新日期:2020-04-01 00:00:00
abstract:CONCLUSION:Videomicroscopy with subpixel analysis is an excellent system for quantification of outer hair cell (OHC) movements. The resolution of a few nanometers is accurate enough to show induced differences of electromotility. OBJECTIVE:Electromotility of OHCs is a voltage-dependent process resulting from a membran...
journal_title:Acta oto-laryngologica
pub_type: 杂志文章
doi:10.1080/00016480701411502
更新日期:2008-03-01 00:00:00
abstract:BACKGROUND:The most crucial clinical problem of Eosinophilic Otitis Media (EOM) is sensorineural hearing loss. A previous report revealed that repeated antigen stimulation of middle ear causes eosinophilic inflammation not only in the middle ear but also in the inner ear. OBJECTIVE:The purpose of the present study was...
journal_title:Acta oto-laryngologica
pub_type: 杂志文章
doi:10.1080/00016489.2016.1266508
更新日期:2017-05-01 00:00:00
abstract:CONCLUSION:The average recovery of hearing and cessation of tinnitus was significantly better after treatment with Radix Astragali (RA) than after non-treatment with RA. RA can be valuable adjuvant therapy for patients with acute acoustic trauma (AAT). OBJECTIVES:AAT is one of the early indications for the use of RA. ...
journal_title:Acta oto-laryngologica
pub_type: 临床试验,杂志文章
doi:10.3109/00016489.2011.591823
更新日期:2011-10-01 00:00:00
abstract::Life-threatening cervical complications associated with infectious mononucleosis are rare. The combination of acute epiglottitis and subsequent necrotizing fasciitis of the head and neck in a patient with infectious mononucleosis has not been reported to date.A 47-year-old female with infectious mononucleosis and epig...
journal_title:Acta oto-laryngologica
pub_type: 杂志文章
doi:10.1080/00016480510027475
更新日期:2005-10-01 00:00:00
abstract::The tonsils are mucosa-associated lymphoid organs. Several details regarding their normal function and pathological alteration are still unknown. In this review the relationship of the tonsils to similar lymphoid organs is discussed, the possibilities of antigen uptake (M cells and micropores of the crypt epithelium) ...
journal_title:Acta oto-laryngologica
pub_type: 杂志文章,评审
doi:
更新日期:1987-05-01 00:00:00
abstract::Dose-effect curves for inhibition of growth of P388/P mouse lymphoma cells by ethacrynic acid and kanamycin used alone and in combination were determined in vitro. Ethacrynic acid was 600 times more potent than kanamycin and combinations of the drugs resulted in overall additive effects. These results were compared wi...
journal_title:Acta oto-laryngologica
pub_type: 杂志文章
doi:10.3109/00016487909126389
更新日期:1979-01-01 00:00:00
abstract:CONCLUSIONS:We used a feedback method, based on a blood pressure manometer with inflatable cuff, to control the sternocleidomastoid muscle (SCM) contraction. To obtain comparable left-right VEMP responses, it is necessary (1) to determine which cuff pressures on both sides yield identical mean rectified voltage (MRV) v...
journal_title:Acta oto-laryngologica
pub_type: 杂志文章
doi:10.1080/00016480500527227
更新日期:2006-08-01 00:00:00
abstract:CONCLUSIONS:The distortion product otoacoustic emission (DPOAE) amplitudes in diabetics were significantly lower than those in controls when pure tone audiometry (PTA) was within normal limits, implying cochlear function impairment in the early stage of diabetes mellitus. Retrocochlear damage was suggested by elongated...
journal_title:Acta oto-laryngologica
pub_type: 杂志文章,meta分析,评审
doi:10.1080/00016489.2016.1223344
更新日期:2017-02-01 00:00:00
abstract::Background: If the pulsatile tinnitus caused by sigmoid sinus diverticulum/dehiscence cannot be diagnosed and treated, it can lead to significant morbidity and mortality.Aim: To assess the sandwich surgical technique for sigmoid sinus wall reconstruction for the treatment of pulsatile tinnitus caused by sigmoid sinus ...
journal_title:Acta oto-laryngologica
pub_type: 杂志文章
doi:10.1080/00016489.2019.1668960
更新日期:2019-12-01 00:00:00
abstract::Acid base balance changes were observed during 72 h following bilateral nasal obstruction in rats. Mouth breathing caused acute respiratory acidosis and marked aerophagia, leading to spontaneous death of the experimental animal 80 to 90 h postoperatively. Stenotic oropharyngeal airway, due to palatal-epiglottic approx...
journal_title:Acta oto-laryngologica
pub_type: 杂志文章
doi:10.3109/00016489109137406
更新日期:1991-01-01 00:00:00
abstract:BACKGROUND:Intratympanic corticosteroid (IC), intravenous batroxobin (IB) as the treatment for sudden sensorineural hearing loss (SSNHL) has been reported. However, the data on combination therapy (CT) was scarce. OBJECTIVE:The aim of this retrospective study was to compare the efficacy of IC, IB, and CT in the treatm...
journal_title:Acta oto-laryngologica
pub_type: 杂志文章
doi:10.1080/00016489.2019.1592221
更新日期:2019-06-01 00:00:00
abstract::The feasibility of furosemide test for the detection of endolymphatic hydrops has previously been discussed (Authors, 1973, 1975). The glycerol test also has been reported as being effective for the same purpose but only in Meniere patients with fluctuating hearing loss (Klockhoff & Lindblom, 1966). In 48 patients wit...
journal_title:Acta oto-laryngologica
pub_type: 杂志文章
doi:10.3109/00016487709128845
更新日期:1977-03-01 00:00:00
abstract:CONCLUSION:Otolith organs input influences the axis of horizontal semicircular canal ocular reflex; therefore, the plane of compensatory eye movements induced by the horizontal canal stimulation is not always parallel to the canal. OBJECTIVE:To clarify whether horizontal canal ocular reflex is influenced by otolith or...
journal_title:Acta oto-laryngologica
pub_type: 杂志文章
doi:10.3109/00016489.2012.656286
更新日期:2012-07-01 00:00:00
abstract:CONCLUSION:Chronic subjective dizziness (CSD) is frequent and affects twice as many women as men. Anxiety is a strong predisposing factor. The pathophysiologic concept of this disorder assumes that balance function and emotion share common neurologic pathways, which might explain that the balance disorder can provoke f...
journal_title:Acta oto-laryngologica
pub_type: 杂志文章
doi:10.1080/00016480701805455
更新日期:2008-10-01 00:00:00
abstract::The vascular pattern of the rat cochlea was demonstrated by the aid of Berlin Blue (Prussian Blue) contrast. The vascular anatomy is similar to other mammals. The infrequent occurrence of the vessel of the basilar membrane under the organ of Corti is interesting in respect to its presumed importance for the oxygen sup...
journal_title:Acta oto-laryngologica
pub_type: 杂志文章
doi:10.3109/00016488009127102
更新日期:1980-01-01 00:00:00
abstract::Localization of elements at the cellular and sub-cellular levels was performed with the energy dispersive X-ray microanalysis technique, using shock-frozen, freeze-dried and araldite-embedded mouse (CBA/CBA) cochleae sectioned dry. Anatomical identification occurred in the STEM (scanning transmission electron microsco...
journal_title:Acta oto-laryngologica
pub_type: 杂志文章
doi:10.3109/00016488409107585
更新日期:1984-11-01 00:00:00
abstract::The trachea whole organ perfusion technique was used to study the effect of tumor necrosis factor alpha (TNF alpha) and interleukin-1 alpha (IL-1 alpha) on the adherence of otitis media pathogen Streptococcus pneumoniae (Spn) type 6A. Tracheas were removed from chinchillas and divided equally. One-half trachea was act...
journal_title:Acta oto-laryngologica
pub_type: 杂志文章
doi:10.1080/00016489950181981
更新日期:1999-01-01 00:00:00
abstract:CONCLUSION:The use of both gadolinium chloride (GdCl(3)) and osmium tetroxide (OsO(4)) allowed for the visualization of the membranous labyrinth and other intralabyrinthine structures, at different intensities, as compared with the control sample. This initial comparison shows the advantages of GdCl(3) in radiological ...
journal_title:Acta oto-laryngologica
pub_type: 杂志文章
doi:10.3109/00016489.2012.715751
更新日期:2013-01-01 00:00:00
abstract:CONCLUSION:Bilateral electric acoustic stimulation (EAS) effectively improved speech perception in noise and sound localization in patients with high-frequency hearing loss. OBJECTIVE:To evaluate bilateral EAS efficacy of sound localization detection and speech perception in noise in two cases of high-frequency hearin...
journal_title:Acta oto-laryngologica
pub_type: 杂志文章
doi:10.3109/00016489.2014.951453
更新日期:2015-02-01 00:00:00
abstract::We previously reported that long-term exposure to glutamate (Glu) induced death of cochlear outer hair cells (OHCs). However, the mechanisms of OHC death induced by Glu were unclear. In the central nervous system, Glu is known to interfere with a cystine-Glu antiporter, leading to a decrease in cystine uptake and redu...
journal_title:Acta oto-laryngologica
pub_type: 杂志文章
doi:10.1080/00016489950180612
更新日期:1999-01-01 00:00:00
abstract::The embryonic development of the crista ampullaris of the CBA/CBA mouse was followed both in organ culture of explanted inner ears of the 16th gestational day and in vivo from the 16th gestational day until the 21st day, an age corresponding to birth. Cytodifferentiation of the sensory epithelium of the crista ampulla...
journal_title:Acta oto-laryngologica
pub_type: 杂志文章
doi:10.3109/00016488309132868
更新日期:1983-07-01 00:00:00
abstract::Explants of stria vascularis and spiral ligament of guinea pig cochlea were kept in primary culture. On the explant, proliferating marginal cells advanced by 15 microns/day, suggesting that in vivo defects of the strial epithelium can be covered by new marginal cells. The marginal cells growing in the cell culture dis...
journal_title:Acta oto-laryngologica
pub_type: 杂志文章
doi:10.3109/00016489209137471
更新日期:1992-09-01 00:00:00
abstract::An antimicrobial agent, kanamycin, has been shown to produce as an untoward effect, ototoxicity. The purpose of this study was to investigate differential effects of kanamycin ototoxicity as a function of Rx timing with regard to circadian rhythms. Four groups of comparable weight Sprague-Dawley rats received a daily ...
journal_title:Acta oto-laryngologica
pub_type: 杂志文章
doi:10.3109/00016489109100749
更新日期:1991-01-01 00:00:00
abstract:CONCLUSION:Given the phonetic balancing across lists and the validation with spectrally degraded speech, the present Mandarin disyllable recognition test (DRT) materials may be useful for assessing speech performance of Mandarin-speaking cochlear implant (CI) users. If combined with the previously developed sentence ma...
journal_title:Acta oto-laryngologica
pub_type: 杂志文章
doi:10.3109/00016489.2011.653668
更新日期:2012-08-01 00:00:00
abstract::Background: Frequency-specific hearing studies are important for predicting hearing results and the prognosis after stapes surgery, to prepare for the rehabilitation of frequency-specific hearing in patients with otosclerosis.Objectives: To evaluate outcomes of stapes surgery of Chinese otosclerosis patients with diff...
journal_title:Acta oto-laryngologica
pub_type: 杂志文章
doi:10.1080/00016489.2020.1724331
更新日期:2020-05-01 00:00:00
abstract:BACKGROUND:Benign paroxysmal positional vertigo (BPPV) is the most common type of peripheral vertigo. This study aimed to evaluate the effects of the Semont maneuver (SM) for BPPV treatment, compared with other methods. METHODS:Studies were selected in relevant databases under pre-defined criteria up to June 2015. The...
journal_title:Acta oto-laryngologica
pub_type: 杂志文章,meta分析
doi:10.1080/00016489.2016.1212265
更新日期:2017-01-01 00:00:00