Polypeptide composition and an immunological analysis of DNA methyltransferases from different species.

abstract：:Tetrahydrobiopterin plays an important role in the biosynthesis of certain neurotransmitters. Using DEAE-Sepharose FF column chromatography, we separated the enzyme that synthesizes tetrahydrobiopterin from 6-pyruvoyl-tetrahydropterin [which is different from sepiapterin reductase (EC 1.1.1.153)] in the lemon mutant o...

journal_title：Archives of biochemistry and biophysics

authors： Iino T,Takikawa SI,Yamamoto T,Sawada H

更新日期：2000-01-15 00:00:00

abstract：:We previously found that vitamin K3 (menadione, 2-methyl-1,4-naphthoquinone) inhibits the activity of human mitochondrial DNA polymerase (pol) γ. In this study, we focused on plumbagin (5-hydroxy-2-methyl-1,4-naphthoquinone), and chemically synthesized novel plumbagins conjugated with C2:0 to C22:6 fatty acids (5-O-ac...

journal_title：Archives of biochemistry and biophysics

authors： Onodera T,Kuriyama I,Sakamoto Y,Kawamura M,Kuramochi K,Tsubaki K,Tabata A,Naganune H,Mizushina Y

更新日期：2015-05-01 00:00:00

abstract：:Pseudonaja textilis, an Australian Elapid, is known to produce a highly toxic venom. Both protein profiling and N-terminal sequence analysis showed the presence of four new phospholipases A(2) in this venom. Besides being non-lethal, the phospholipase A(2) proteins were found to be moderately active enzymes and they s...

journal_title：Archives of biochemistry and biophysics

authors： Armugam A,Gong N,Li X,Siew PY,Chai SC,Nair R,Jeyaseelan K

更新日期：2004-01-01 00:00:00

abstract：:The roles of p67-deglycosylase (p67-DG) in the regulation of protein synthesis in baculovirus-infected insect cells were studied. Like vaccinia viral infection, baculovirus infection of insect cells also induced the appearance of a p67-DG. However, p67-DG activity could not be detected because these cells do not conta...

journal_title：Archives of biochemistry and biophysics

authors： Saha D,Wu S,Bose A,Chatterjee N,Chakraborty A,Chatterjee M,Gupta NK

更新日期：1997-06-15 00:00:00

abstract：:ATP-activated P2Y receptors play an important role in renal sodium excretion. The aim of this study was to evaluate the modulation of ATPase-driven sodium reabsorption in the proximal tubule by ATP or adenosine (Ado). LLC-PK1 cells, a model of porcine proximal tubule cells, were used. ATP (10(-6)M) or Ado (10(-6)M) sp...

journal_title：Archives of biochemistry and biophysics

authors： Wengert M,Ribeiro MC,Abreu TP,Coutinho-Silva R,Leão-Ferreira LR,Pinheiro AA,Caruso-Neves C

更新日期：2013-07-15 00:00:00

abstract：:Peroxisome proliferator-activated receptor alpha (PPARα) has been implicated in the pathogenesis of cardiac hypertrophy, although its mechanism of action remains largely unknown. To determine the effect of PPARα activation on endothelin-1 (ET-1)-induced cardiomyocyte hypertrophy and explore its molecular mechanisms, w...

journal_title：Archives of biochemistry and biophysics

authors： Le K,Li R,Xu S,Wu X,Huang H,Bao Y,Cai Y,Lan T,Moss J,Li C,Zou J,Shen X,Liu P

更新日期：2012-02-01 00:00:00

abstract：:Carbamoyl phosphate synthetase I (CPSase I) catalyzes the first reaction of the urea cycle in mammalian ureotelic species. The positive allosteric cofactor N-acetyl-L-glutamate (AGA) is required for CPSase I activity and is important for regulation of the urea cycle. A similar enzyme, CPSase III, catalyzes this reacti...

journal_title：Archives of biochemistry and biophysics

authors： Julsrud EA,Walsh PJ,Anderson PM

更新日期：1998-02-01 00:00:00

abstract：:The earthworm-derived chemoattractant ES20 interacts with its G-protein-coupled receptors on the plasma membrane of vomeronasal (VN) sensory neurons of garter snakes, resulting in an increase in inositol trisphosphate [J. Biol. Chem. 269 (1994) 16867] and a rapid phosphorylation of the membrane-bound proteins, p42/44 ...

journal_title：Archives of biochemistry and biophysics

authors： Wang D,Liu W,Liu J,Chen P,Quan W,Halpern M

更新日期：2002-12-15 00:00:00

abstract：:Coupling of the narrow 311-nm Gd3+ fluorescence transition with the vibrational transitions of its inner-sphere ligands gives rise to the emission of vibronic photons between 320 and 350 nm. The energy difference between the fluorescence and vibronic transitions is equal to that of the vibrational transitions of the m...

journal_title：Archives of biochemistry and biophysics

authors： MacGregor RB Jr

更新日期：1989-10-01 00:00:00

abstract：:FHRE-Luc is a promoter reporter construct that is widely used to assess the activity of FoxO (forkhead box, class O) transcription factors. We here demonstrate that this promoter construct responds to exposure of HepG2 human hepatoma cells to known agonists of the aryl hydrocarbon receptor (AhR), 3-methylcholanthrene,...

journal_title：Archives of biochemistry and biophysics

authors： Eckers A,Sauerbier E,Anwar-Mohamed A,Hamann I,Esser C,Schroeder P,El-Kadi AO,Klotz LO

更新日期：2011-12-15 00:00:00

abstract：:Endometrial carcinoma is a type of gynecological cancer that originates in the endometrial epithelial tissue. Due to its high proliferation and ability to invade muscle tissue, it is one of the most common malignant tumors in the female reproductive system. Fatostatin is a small molecule non-sterol diarylthiazole deri...

journal_title：Archives of biochemistry and biophysics

authors： Yao L,Chen S,Li W

更新日期：2020-05-15 00:00:00

abstract：:Diquat is a hepatotoxin whose toxicity in vivo and in vitro is mediated by redox cycling and greatly enhanced by pretreatment with 1,3-bis(2-chloroethyl)-1-nitrosourea (BCNU), an inhibitor of glutathione reductase. The mechanism by which redox cycling mediates diquat cytotoxicity is unclear, however. Here, we have att...

journal_title：Archives of biochemistry and biophysics

authors： Sandy MS,Moldeus P,Ross D,Smith MT

更新日期：1987-11-15 00:00:00

abstract：:The effects of nonlamellar-prone lipids, diacylglycerol and phosphatidylethanolamine (PE), on the kinetic association of SecA with model membranes were examined by measuring changes in the intrinsic emission fluorescence with a stopped-flow apparatus. Upon interaction with standard liposomes composed of 50 mol% dioleo...

journal_title：Archives of biochemistry and biophysics

authors： Ahn T,Kim JS,Lee BC,Yun CH

更新日期：2001-11-01 00:00:00

abstract：:Human sorcin, M(r) 22-kDa/pI 5.3, was identified in HOB1 lymphoma cells resistant to 1.0 microM vincristine and polyclonal antibody against it was produced. Using antibody to precipitate sorcin from 32P-labeled cell extract, the SDS-PAGE-resolved human sorcin was not phosphorylated in contrast to the report of A. M. v...

journal_title：Archives of biochemistry and biophysics

authors： Lee WP

更新日期：1996-01-15 00:00:00

abstract：:To elucidate the physiological significance of ketone bodies on insulin and glucagon secretion, the direct effects of beta-hydroxybutyrate (BOHB) and acetoacetate (AcAc) infusion on insulin and glucagon release from perfused rat pancreas were investigated. The BOHB or AcAc was administered at concentrations of 10, 1, ...

journal_title：Archives of biochemistry and biophysics

authors： Ikeda T,Yoshida T,Ito Y,Murakami I,Mokuda O,Tominaga M,Mashiba H

更新日期：1987-08-15 00:00:00

abstract：:We analyzed the effect of a natural osmolyte, trimethylamine N-oxide (TMAO), on structural properties and conformational stabilities of several proteins under macromolecular crowding conditions by a set of biophysical techniques. We also used the solvent interaction analysis method to look at the peculiarities of the ...

journal_title：Archives of biochemistry and biophysics

authors： Breydo L,Sales AE,Ferreira L,Fedotoff O,Shevelyova MP,Permyakov SE,Kroeck KG,Permyakov EA,Zaslavsky BY,Uversky VN

更新日期：2015-03-15 00:00:00

abstract：:In cells expressing only the Glut1 isoform of glucose transporters, we have shown that glucose transport is markedly stimulated in response to hypoxia or inhibition of oxidative phosphorylation, conditions that would be expected to cause a stimulation of AMP-activated protein kinase (AMPK) activity. In the present stu...

journal_title：Archives of biochemistry and biophysics

authors： Abbud W,Habinowski S,Zhang JZ,Kendrew J,Elkairi FS,Kemp BE,Witters LA,Ismail-Beigi F

更新日期：2000-08-15 00:00:00

abstract：:GAPDH was speculated to function as a transient trap to reduce the potential toxicity of free heme by a specific and reversible binding with heme. Up to now, there has been lack of studies focused on this effect. In this paper, the efficiency of GAPDH-heme complex on catalyzing protein carbonylation and nitration, the...

journal_title：Archives of biochemistry and biophysics

authors： Huang Y,Zhang P,Yang Z,Wang P,Li H,Gao Z

更新日期：2017-04-01 00:00:00

abstract：:A mixture of phosphorylated manno-oligosaccharides was isolated from the acid-stable domain of phosphomannan of Candida albicans NIH B-792 strain (serotype B) by acetolysis and was fractionated on a column of Bio-Gel P-2 equilibrated with 50 mM pyridine-CH3COOH buffer, pH 5.0. A monophosphorylated mannotetraose was is...

journal_title：Archives of biochemistry and biophysics

authors： Shibata N,Kobayashi H,Takahashi S,Okawa Y,Hisamichi K,Suzuki S,Suzuki S

更新日期：1991-11-01 00:00:00

abstract：:Prostasomes are membranous vesicles (150-200 nm diameter) present in human semen. They are secreted by the prostate gland and contain large amounts of cholesterol, sphingomyelin, and Ca2+. In addition, some of their proteins are enzymes. Prostasomes enhance the motility of ejaculated sperm and are involved in a number...

journal_title：Archives of biochemistry and biophysics

authors： Carlini E,Palmerini CA,Cosmi EV,Arienti G

更新日期：1997-07-01 00:00:00

abstract：:Using Escherichia coli guanosine triphosphate cyclohydrolase, dihydroneopterin triphosphate was synthesized from guanosine triphosphate and was compared with sepiapterin as a substrate for tetrahydrobiopterin formation in bovine adrenal medulla extracts. The dihydrofolate reductase inhibitor, methotrexate, blocks the ...

journal_title：Archives of biochemistry and biophysics

authors： Smith GK,Nichol CA

更新日期：1983-11-01 00:00:00

abstract：:The reaction of the Cu,Co derivative of bovine Cu,Zn superoxide dismutase with phenylglyoxal or butanedione, which are known to inactivate the enzyme by selectively binding to Arg 141, has been studied by 1H NMR. Several 1H NMR lines of the copper-liganding histidine residues were perturbed, reproducing an effect so f...

journal_title：Archives of biochemistry and biophysics

authors： Paci M,Desideri A,Sette M,Rotilio G

更新日期：1991-04-01 00:00:00

abstract：:Alanine:glyoxylate aminotransferase has been reported to be present as the apo enzyme in the peroxisomes and as the holo enzyme in the mitochondria in chick (white leghorn) embryonic liver. However, surprisingly, birds were found to be classified into two groups on the basis of intraperoxisomal forms of liver alanine:...

journal_title：Archives of biochemistry and biophysics

authors： Sakuraba H,Fujiwara S,Noguchi T

更新日期：1991-05-01 00:00:00

abstract：:Peroxisomes contain oxidases that produce H(2)O(2), which can result in protein oxidation. To test the vulnerability of peroxisomal proteins to oxidation in vivo the organelles were isolated from castor bean endosperm incubated with H(2)O(2). When peroxisomes were exposed to H(2)O(2)in vivo, the peroxisomal proteins e...

journal_title：Archives of biochemistry and biophysics

authors： Anand P,Kwak Y,Simha R,Donaldson RP

更新日期：2009-11-01 00:00:00

abstract：:Catechol 1,2-dioxygenase catalyzes the oxygenative ring cleavage of catechol to form cis,cis-muconic acid and is encoded by a catA gene. We have cloned a catA gene from Pseudomonas putida mt-2 using a PCR product of amino acid sequence-based primers as a probe. The amino acid sequence deduced from the 930 nucleotides ...

journal_title：Archives of biochemistry and biophysics

authors： Nakai C,Uyeyama H,Kagamiyama H,Nakazawa T,Inouye S,Kishi F,Nakazawa A,Nozaki M

更新日期：1995-08-20 00:00:00

abstract：:Previous work has shown that catalytic function is associated exclusively with the C-terminal half of the Type I isozyme of mammalian hexokinase. In contrast, we now demonstrate that both halves of the Type II isozyme possess comparable catalytic activities. Mutation of a catalytically important Ser residue to Ala at ...

journal_title：Archives of biochemistry and biophysics

authors： Tsai HJ,Wilson JE

更新日期：1996-05-01 00:00:00

abstract：:To identify the novel inhibitor of de novo lipogenesis in hepatocytes, we screened for inhibitory activity of triglyceride (TG) synthesis using [14C]acetate in the human hepatoma cell line, HepG2. Using this assay system we discovered the novel compound, benzofuranyl alpha-pyrone (TEI-B00422). TEI-B00422 also inhibite...

journal_title：Archives of biochemistry and biophysics

authors： Sugimoto Y,Naniwa Y,Nakamura T,Kato H,Yamamoto M,Tanabe H,Inoue K,Imaizumi A

更新日期：2007-12-01 00:00:00

abstract：:Butyric acid produces multiple effects on mammalian cells in culture, including alterations in morphology, depression of growth rate, increased histone acetylation, and modified production of various proteins and enzymes. The latter effect is exemplified by the induction in HeLa cells of the glycoprotein hormone alpha...

journal_title：Archives of biochemistry and biophysics

authors： McClure DS,Cox GS

更新日期：1984-08-15 00:00:00

abstract：:A current hypothesis explaining the aging process implicates the accumulation of oxidized protein in animal tissues. This is primarily based on a series of reports showing an age-dependent increase in protein carbonyl content and an age-dependent decrease in the activities of enzymes, especially of alkaline proteases,...

journal_title：Archives of biochemistry and biophysics

authors： Cao G,Cutler RG

更新日期：1995-06-20 00:00:00

abstract：:The susceptibility of 40S and 60S ribosomal subunits from Saccharomyces cerevisiae to digestion with varying concentrations of trypsin was studied by two-dimensional electrophoresis and quantitative measurements of the protein remaining with the ribosomal particles after trypsin treatment. Proteins from both subunits ...

journal_title：Archives of biochemistry and biophysics

authors： Lee JC,Haley WT Jr

更新日期：1983-07-01 00:00:00