Modulation of salt transport rate affects DNA synthesis in vivo in rat renal tubules.


:In adult male Wistar rats we investigated whether cell proliferation contributes to salt load-induced hypertrophy of distal tubules. In one treatment group salt transport in the thick ascending limb (TAL) was inhibited by furosemide (7.5 mg/100 g body wt/24 hr, via osmotic minipump) and stimulated in the successive distal segments by simultaneous high salt intake (F + Salt). Controls without furosemide treatment had a standard salt intake. All animals received the thymidine analog bromodeoxyuridine (BrdU) during 24 and 72 hours, respectively. In cryostat sections of the perfusion-fixed kidneys DNA synthesis was assessed by immunohistochemistry for BrdU, and for endogenous proliferating cellular nuclear antigen (PCNA). Incidence of BrdU- and PCNA-labeled nuclei were quantified in proximal tubules, medullary TAL, and cortical distal segments downstream the TAL. In control animals low labeling indices were found in all investigated segments. After 24 and 72 hours of F + Salt, indices of labeled nuclei were markedly increased in distal segments downstream the TAL, whereas they were significantly reduced in TAL. In proximal tubules increased DNA synthesis rate was apparent only after 72 hours. The data demonstrate that (1.) DNA synthesis rate in nephron segments in vivo varies in parallel with changes of their salt transport activity; (2.) increased DNA synthesis, thus probably cellular proliferation, is a component of the structural response of nephron segments following increased salt transport activity.


Kidney Int


Kidney international


Loffing J,Le Hir M,Kaissling B




Has Abstract


1995-06-01 00:00:00














  • Clinical trial of focal segmental glomerulosclerosis in children and young adults.

    abstract::This NIH-funded multicenter randomized study of focal segmental glomerulosclerosis (FSGS) treatment compared the efficacy of a 12-month course of cyclosporine to a combination of oral pulse dexamethasone and mycophenolate mofetil in children and adults with steroid-resistant primary FSGS. Of the 192 patients enrolled,...

    journal_title:Kidney international

    pub_type: 杂志文章,多中心研究,随机对照试验


    authors: Gipson DS,Trachtman H,Kaskel FJ,Greene TH,Radeva MK,Gassman JJ,Moxey-Mims MM,Hogg RJ,Watkins SL,Fine RN,Hogan SL,Middleton JP,Vehaskari VM,Flynn PA,Powell LM,Vento SM,McMahan JL,Siegel N,D'Agati VD,Friedman AL

    更新日期:2011-10-01 00:00:00

  • Hypertonic activation of the renal betaine/GABA transporter is microtubule dependent.

    abstract:BACKGROUND:Epithelial cells in the renal inner medulla accumulate osmolytes such as betaine to maintain normal cell volume during prolonged extracellular hypertonic stress. Betaine accumulation is the result of activation of transcription of the BGT1 transporter gene followed by increased betaine transport. METHODS:We...

    journal_title:Kidney international

    pub_type: 杂志文章


    authors: Basham JC,Chabrerie A,Kempson SA

    更新日期:2001-06-01 00:00:00

  • Treatment of experimental renal osteodystrophy with pamidronate.

    abstract::We evaluated the effects of the bisphosphonate pamidronate on bone histomorphometry, structure and strength in male rats with uninephrectomy or with chronic renal disease induced by 5/6 nephrectomy. In rats with chronic renal disease the plasma urea, phosphate and parathyroid hormone levels were significantly increase...

    journal_title:Kidney international

    pub_type: 杂志文章


    authors: Jokihaara J,Pörsti IH,Kööbi P,Jolma PM,Mustonen JT,Saha HH,Sievänen H,Kannus P,Iwaniec UT,Turner RT,Järvinen TL

    更新日期:2008-08-01 00:00:00

  • Adiponectin in renal disease: relationship to phenotype and genetic variation in the gene encoding adiponectin.

    abstract:BACKGROUND:The prevalence of cardiovascular disease (CVD) and inflammation is high in patients with end-stage renal disease (ESRD). Adiponectin is an adipocytokine that may have significant anti-inflammatory and anti-atherosclerotic effects. Low adiponectin levels have previously been found in patients with high risk f...

    journal_title:Kidney international

    pub_type: 杂志文章


    authors: Stenvinkel P,Marchlewska A,Pecoits-Filho R,Heimbürger O,Zhang Z,Hoff C,Holmes C,Axelsson J,Arvidsson S,Schalling M,Barany P,Lindholm B,Nordfors L

    更新日期:2004-01-01 00:00:00

  • A molecular morphometric approach to diabetic kidney disease can link structure to function and outcome.

    abstract::Diabetic kidney disease is the leading cause of kidney failure. However, studies of molecular mechanisms of early kidney damage are lacking. Here we examined for possible linkage between transcriptional regulation and quantitative structural damage in early diabetic kidney disease in Pima Indians with type 2 diabetes....

    journal_title:Kidney international

    pub_type: 杂志文章


    authors: Nair V,Komorowsky CV,Weil EJ,Yee B,Hodgin J,Harder JL,Godfrey B,Ju W,Boustany-Kari CM,Schwarz M,Lemley KV,Nelson PJ,Nelson RG,Kretzler M

    更新日期:2018-02-01 00:00:00

  • Effect of acute increases in filtered HCO3- on renal hydrogen transporters: II. H(+)-ATPase.

    abstract::Adaptive increases in renal bicarbonate reabsorption occur in response to acute increases in filtered bicarbonate (FLHCO3). In a previous study, we showed that an increase in FLHCO3 induced by plasma volume expansion increased the Vmax for Na+/H+ exchange activity in renal cortical brush border membrane vesicles (BBMV...

    journal_title:Kidney international

    pub_type: 杂志文章


    authors: Maddox DA,Barnes WD,Gennari FJ

    更新日期:1997-08-01 00:00:00

  • Nitric oxide enhances paracellular permeability of opossum kidney cells.

    abstract:BACKGROUND:Nitric oxide (NO) has been shown to be a paracrine/autocrine regulator of proximal tubular transport. In this study, we investigated the effect of NO on the paracellular permeability of opossum kidney (OK) cells, a proximal tubule cell line that possesses a leaky paracellular pathway resembling that of the i...

    journal_title:Kidney international

    pub_type: 杂志文章


    authors: Liang M,Knox FG

    更新日期:1999-06-01 00:00:00

  • National trends in acute kidney injury requiring dialysis in England between 1998 and 2013.

    abstract::Acute kidney injury (AKI) severe enough to require dialysis is increasing and associated with high mortality, yet robust information about temporal epidemiology of AKI requiring dialysis in England is lacking. In this retrospective observational study of the Hospital Episode Statistics (HES) data set covering the enti...

    journal_title:Kidney international

    pub_type: 杂志文章


    authors: Kolhe NV,Muirhead AW,Wilkes SR,Fluck RJ,Taal MW

    更新日期:2015-11-01 00:00:00

  • The risks of vascular access.

    abstract::Fatal vascular access hemorrhage is considered a rare complication of hemodialysis (HD). Ellingson et al. indicate otherwise, and their data suggest that it causes 0.4-1.6% of deaths in US HD patients. It is more common with grafts than fistulas, and many victims have had previous access hemorrhages. The widespread pr...

    journal_title:Kidney international

    pub_type: 评论,杂志文章


    authors: Blake PG,Quinn RR,Oliver MJ

    更新日期:2012-09-01 00:00:00

  • The soluble urokinase receptor is not a clinical marker for focal segmental glomerulosclerosis.

    abstract::The soluble urokinase receptor (suPAR) promotes proteinuria and induces focal segmental glomerulosclerosis (FSGS)-like lesions in mice. A serum suPAR concentration cutoff of 3000 pg/ml has been proposed as a clinical biomarker for patients with FSGS. Interestingly, several studies in patients with glomerulopathy found...

    journal_title:Kidney international

    pub_type: 杂志文章


    authors: Meijers B,Maas RJ,Sprangers B,Claes K,Poesen R,Bammens B,Naesens M,Deegens JK,Dietrich R,Storr M,Wetzels JF,Evenepoel P,Kuypers D

    更新日期:2014-03-01 00:00:00

  • Substrate oxidation by isolated single nephron segments of the rat.

    abstract::Substrate oxidation was assessed by measuring 14CO2 production from 14C-labeled substrates in proximal convoluted tubules (PCT), medullary (MTAL), and cortical (CTAL) thick ascending limb of Henle, nephron segments rich in mitochondria and characterized by active solute transport. PCT, MTAL, and CTAL were dissected fr...

    journal_title:Kidney international

    pub_type: 杂志文章


    authors: Klein KL,Wang MS,Torikai S,Davidson WD,Kurokawa K

    更新日期:1981-07-01 00:00:00

  • Classical pathway of complement activation in normal and diseased human glomeruli.

    abstract::Monoclonal antibodies (mAb) reactive against complement components involved in the classical activation pathway were applied in an indirect immunoperoxidase technique for the histological study of normal and diseased human renal tissues. Prominent staining with antibodies against the C4d fragment was seen in all glome...

    journal_title:Kidney international

    pub_type: 杂志文章


    authors: Zwirner J,Felber E,Herzog V,Riethmüller G,Feucht HE

    更新日期:1989-12-01 00:00:00

  • Incremental prognostic value of kidney function decline over coronary artery disease for cardiovascular event prediction after coronary computed tomography.

    abstract::It is unknown whether mild chronic kidney disease (CKD) is associated with adverse cardiovascular (CV) prognosis after accounting for coronary artery disease (CAD). Here we evaluated the interplay between CKD and CAD in predicting CV death or myocardial infarction (MI) and all-cause death. We included 1541 consecutive...

    journal_title:Kidney international

    pub_type: 杂志文章


    authors: Bittencourt MS,Hulten EA,Ghoshhajra B,Abbara S,Murthy VL,Divakaran S,Nasir K,Gowdak LH,Riella LV,Chiumiento M,Hoffmann U,Di Carli MF,Blankstein R

    更新日期:2015-07-01 00:00:00

  • Signal regulatory protein-α interacts with the insulin receptor contributing to muscle wasting in chronic kidney disease.

    abstract::Insulin resistance from chronic kidney disease (CKD) stimulates muscle protein wasting but mechanisms causing this resistance are controversial. To help resolve this, we used microarray analyses to identify initiators of insulin resistance in the muscles of mice with CKD, glucose intolerance, and insulin resistance. C...

    journal_title:Kidney international

    pub_type: 杂志文章


    authors: Thomas SS,Dong Y,Zhang L,Mitch WE

    更新日期:2013-08-01 00:00:00

  • Canonical Wnt/β-catenin signaling mediates transforming growth factor-β1-driven podocyte injury and proteinuria.

    abstract::Transforming growth factor-β1 (TGF-β1) upregulation occurs in virtually all chronic kidney diseases and is associated with podocyte injury and proteinuria; however, the mechanisms contributing to this in vivo are ambiguous. In vitro, incubation of podocytes with TGF-β1 induced Wnt1 expression, β-catenin activation, an...

    journal_title:Kidney international

    pub_type: 杂志文章


    authors: Wang D,Dai C,Li Y,Liu Y

    更新日期:2011-12-01 00:00:00

  • Rat mesangial cells in vitro synthesize a spectrum of proteoglycan species including those of the basement membrane and interstitium.

    abstract::Accumulation of extracellular matrix within the mesangium is an important event in the development of glomerular disease. In this report we have used indirect immunofluorescence to positively identify a number of constituents of the mesangial matrix synthesized by rat mesangial cells (RMC) in vitro including laminin, ...

    journal_title:Kidney international

    pub_type: 杂志文章


    authors: Thomas GJ,Shewring L,McCarthy KJ,Couchman JR,Mason RM,Davies M

    更新日期:1995-10-01 00:00:00

  • Regulation of human renin gene promoter activity: a new negative regulatory region determines the responsiveness to TNF alpha.

    abstract:BACKGROUND:The renin-angiotensin system has been known to regulate blood pressure and body fluid homeostasis. Several lines of evidence have shown that renin gene expression and release are up-regulated by beta-adrenergic stimulation, sodium depletion, and angiotensin converting enzyme inhibition, but down-regulated by...

    journal_title:Kidney international

    pub_type: 杂志文章


    authors: Chen LS,Cuddy MP,LaVallette LA

    更新日期:1998-12-01 00:00:00

  • Risk HLA class II alleles and amino acid residues in myeloperoxidase-ANCA-associated vasculitis.

    abstract::A genome-wide association study (GWAS) indicated that myeloperoxidase-ANCA associated vasculitis (AAV) is associated with HLA-DQ. However, susceptibility alleles in these loci have been under-investigated. Here we genotyped 258 Chinese patients with myeloperoxidase-AAV and 597 healthy control individuals at HLA DRB1, ...

    journal_title:Kidney international

    pub_type: 杂志文章


    authors: Wang HY,Cui Z,Pei ZY,Fang SB,Chen SF,Zhu L,Chen M,Chen N,Zhao MH

    更新日期:2019-10-01 00:00:00

  • Malignancy-related mortality following kidney transplantation is common.

    abstract::There is a paucity of studies describing malignancy-related mortality after kidney transplantation. To help quantify this, we extracted data for all kidney-alone transplant procedures performed in England between April 2001 and March 2012. Data linkage analysis was performed between Hospital Episode Statistics and the...

    journal_title:Kidney international

    pub_type: 杂志文章


    authors: Farrugia D,Mahboob S,Cheshire J,Begaj I,Khosla S,Ray D,Sharif A

    更新日期:2014-06-01 00:00:00

  • The loss of Krüppel-like factor 15 in Foxd1+ stromal cells exacerbates kidney fibrosis.

    abstract::Large epidemiological studies clearly demonstrate that multiple episodes of acute kidney injury contribute to the development and progression of kidney fibrosis. Although our understanding of kidney fibrosis has improved in the past two decades, we have limited therapeutic strategies to halt its progression. Myofibrob...

    journal_title:Kidney international

    pub_type: 杂志文章


    authors: Gu X,Mallipattu SK,Guo Y,Revelo MP,Pace J,Miller T,Gao X,Jain MK,Bialkowska AB,Yang VW,He JC,Mei C

    更新日期:2017-11-01 00:00:00

  • Regulation of transforming growth factor-beta1 by insulin in prediabetic African Americans.

    abstract::Insulin resistance has been associated with kidney disease even in the absence of diabetes; however, pathways linking insulin resistance to kidney disease are unclear. The purpose of this study was to determine if transforming growth factor (TGF)-beta1, a key cytokine associated with kidney disease, responds to circul...

    journal_title:Kidney international

    pub_type: 杂志文章


    authors: Huan Y,DeLoach S,Daskalakis C,Dunn SR,Sharma K,Falkner B

    更新日期:2010-08-01 00:00:00

  • Intrarenal ghrelin receptors regulate ENaC-dependent sodium reabsorption by a cAMP-dependent pathway.

    abstract::The main distal nephron segment sodium transporters are the distal tubule chlorothiazide-sensitive sodium chloride cotransporter (NCC) and the collecting duct amiloride-sensitive epithelial sodium channel (ENaC). The infusion of ghrelin into the renal interstitium stimulates distal nephron-dependent sodium reabsorptio...

    journal_title:Kidney international

    pub_type: 杂志文章


    authors: Kemp BA,Howell NL,Gildea JJ,Keller SR,Padia SH

    更新日期:2013-09-01 00:00:00

  • Knockout of aminopeptidase A in mice causes functional alterations and morphological glomerular basement membrane changes in the kidneys.

    abstract::Aminopeptidase A is one of the most potent enzymes within the renin-angiotensin system in terms of angiotensin II degradation. Here, we examined whether there is a kidney phenotype and any compensatory changes in other renin angiotensin system enzymes involved in the metabolism of angiotensin II associated with aminop...

    journal_title:Kidney international

    pub_type: 杂志文章


    authors: Marahrens B,Schulze A,Wysocki J,Lin MH,Ye M,Kanwar YS,Bader M,Velez JCQ,Miner JH,Batlle D

    更新日期:2020-12-11 00:00:00

  • Plasminogen activator inhibitor-1 expression is regulated by the angiotensin type 1 receptor in vivo.

    abstract:BACKGROUND:The fibrinolytic system plays an important role in degrading fibrin-rich thrombi and in vascular and tissue remodeling. Elevated levels of plasminogen activator inhibitor-1 (PAI-1) can reduce the efficiency of the endogenous fibrinolytic system. Angiotensin (Ang) has been shown to regulate PAI-1 expression v...

    journal_title:Kidney international

    pub_type: 杂志文章


    authors: Nakamura S,Nakamura I,Ma L,Vaughan DE,Fogo AB

    更新日期:2000-07-01 00:00:00

  • Effect of vitamin D metabolites on calcitriol metabolism in experimental renal failure.

    abstract::Previous studies from our laboratory have demonstrated that metabolic clearance rate (MCR) of calcitriol is decreased in experimental renal failure. In this experiment, we examined the effects of calcitriol, 25-hydroxyvitamin D3 (25(OH)D3) and 24,25-dihydroxyvitamin D3 (24,25(OH)2D3) on the MCR of calcitriol in renal ...

    journal_title:Kidney international

    pub_type: 杂志文章


    authors: Patel S,Simpson RU,Hsu CH

    更新日期:1989-08-01 00:00:00

  • Renal structural abnormalities following recovery from acute puromycin nephrosis.

    abstract:BACKGROUND:Rats that recover from acute puromycin nephrosis later develop widespread glomerular and tubulointerstitial injury. The current study sought to identify structural changes present in the recovery phase that could precipitate progressive renal disease. METHODS:Stereologic studies were performed 10 weeks afte...

    journal_title:Kidney international

    pub_type: 杂志文章


    authors: Rasch R,Nyengaard JR,Marcussen N,Meyer TW

    更新日期:2002-08-01 00:00:00

  • Inhibition of eNOS phosphorylation mediates endothelial dysfunction in renal failure: new effect of asymmetric dimethylarginine.

    abstract::Patients with chronic kidney disease have elevated circulating asymmetric dimethylarginine (ADMA). Recent studies have suggested that ADMA impairs endothelial nitric oxide synthase (eNOS) by effects other than competition with the substrate L-arginine. Here, we sought to identify the molecular mechanism by which incre...

    journal_title:Kidney international

    pub_type: 杂志文章


    authors: Kajimoto H,Kai H,Aoki H,Yasuoka S,Anegawa T,Aoki Y,Ueda S,Okuda S,Imaizumi T

    更新日期:2012-04-01 00:00:00

  • Pregnancy in IgA nephropathy.

    abstract::The impacts of IgA nephropathy and pregnancy on each other were evaluated in 118 women who conceived 168 times between 1970 and 1988. Rates of spontaneous abortion, normal delivery, live birth and perinatal death were 9, 66, 87 and 4%, respectively. Infants born to women with glomerular filtration rates (GFR) lower th...

    journal_title:Kidney international

    pub_type: 杂志文章


    authors: Abe S

    更新日期:1991-12-01 00:00:00

  • Will introduction of tolvaptan change clinical practice in autosomal dominant polycystic kidney disease?

    abstract::The vasopressin inhibitor tolvaptan is clinically effective in slowing growth of renal cysts and reduction in estimated glomerular filtration rate (eGFR) in autosomal dominant polycystic kidney disease (ADPKD), but these effects are mitigated by the associated polyuria. Changes of total kidney volume, eGFR, and sympto...

    journal_title:Kidney international

    pub_type: 评论,杂志文章


    authors: Horie S

    更新日期:2015-07-01 00:00:00

  • Acetate and bicarbonate hemodialysis in patients with and without autonomic dysfunction.

    abstract::To test the contribution of bicarbonate (Bi) to hemodynamic stability during hemodialysis (HD), we compared the effects of ultrafiltration dialysis with bicarbonate and with acetate under conditions of high-sodium (141 mEq/liter) and low-sodium (130 mEq/liter) dialysate concentrations in 12 stable HD patients. Group 1...

    journal_title:Kidney international

    pub_type: 临床试验,杂志文章


    authors: Velez RL,Woodard TD,Henrich WL

    更新日期:1984-07-01 00:00:00