Abstract:
:Activating mutations of G proteins, which are membrane signal transducers, have been associated recently with the development of various endocrine neoplasms. Mutations of two highly conserved codons, Arg201 and Gln227, in the alpha-subunit of the Gs protein, the adenylyl cyclase-stimulating protein, were first described in growth hormone-producing pituitary tumours. They resulted in constitutive activation of the alpha s-subunit by decreasing intrinsic GTPase activity. A similar mutation, affecting codon Arg179 (exon 5) in the alpha-subunit of the Gi2 protein, the adenylyl cyclase-inhibiting protein, has been described by a single group in ovarian and adrenocortical tumours. We evaluated the frequency of activating mutations in the alpha-subunit of the Gi2 protein in 18 human adrenocortical tumours. We screened exons 5 (codon Arg179) and 6 (codon gln205) for mutations by denaturing gradient gel electrophoresis analysis of leucocyte and tumoural DNA. No abnormal migration pattern was found in either exon. The absence of mutation in exon 5, which includes the Arg179 codon, was confirmed in all tumoural DNA by direct sequencing. In conclusion, we did not find any oncogenic mutations in the GTPase domain of the alpha-subunit of the Gi2 protein in adrenocortical tumours. Thus, the previously oncogenic gip2 mutations do not appear to be determinant for adrenocortical tumourigenesis.
journal_name
Eur J Endocrinoljournal_title
European journal of endocrinologyauthors
Gicquel C,Dib A,Bertagna X,Amselem S,Le Bouc Ydoi
10.1530/eje.0.1330166subject
Has Abstractpub_date
1995-08-01 00:00:00pages
166-72issue
2eissn
0804-4643issn
1479-683Xjournal_volume
133pub_type
杂志文章abstract:OBJECTIVE:To evaluate the impact of long-term, non-suppressive levothyroxine (L-T(4)) treatment on quantitative ultrasonometry in women. DESIGN:This was a case-control study. SUBJECTS AND METHODS:Altogether 667 women (mean age+/-s.d., 49.5+/-13.1 years) were studied. Of these, 156 (23%) had non-toxic goitre or hypoth...
journal_title:European journal of endocrinology
pub_type: 杂志文章
doi:10.1530/eje.0.1420445
更新日期:2000-05-01 00:00:00
abstract:CONTEXT:McCune-Albright syndrome (MAS) is a disorder caused by a post-zygotic gain-of-function mutation in the gene encoding the Gs-alpha protein. Sexual precocity, common in girls, has been reported in only 15% of boys, and little is known on the long-term evolution of MAS in males. OBJECTIVE:In a boy with MAS, we st...
journal_title:European journal of endocrinology
pub_type: 杂志文章
doi:10.1530/EJE-07-0494
更新日期:2008-06-01 00:00:00
abstract:OBJECTIVE:Dehydroepiandrosterone (DHEA) is a widely studied steroid hormone with multi-functional properties. Reports suggest that some of the many activities of DHEA are due to its protective effect against lipid peroxidation. Nevertheless, the antioxidant properties of DHEA are still the subject of debate. The aim wa...
journal_title:European journal of endocrinology
pub_type: 杂志文章
doi:10.1530/eje.0.1410035
更新日期:1999-07-01 00:00:00
abstract::Human growth hormone (GH) exists in a variety of isoforms. In the pituitary, the most abundant isoform is 22-kD GH (22 K GH), while other isoforms (non-22 K GH) are present in variable amounts. In human plasma, the GH heterogeneity contributes to the wide variability in GH levels measured by different immunoassays. Th...
journal_title:European journal of endocrinology
pub_type: 杂志文章
doi:10.1530/eje.0.1350573
更新日期:1996-11-01 00:00:00
abstract:CONTEXT AND OBJECTIVE:Adipokines are involved in the etiopathology of obesity-related disorders. Since the role of adipokine retinol-binding protein-4 (RBP4) in obesity remains uncertain and its relationship with other adipokines and inflammatory markers has not been examined in detail, we investigated the relationship...
journal_title:European journal of endocrinology
pub_type: 杂志文章
doi:10.1530/EJE-08-0866
更新日期:2009-07-01 00:00:00
abstract:BACKGROUND:We examined the hypothesis that high FGF-23 levels early after transplantation contribute to the onset of hypophosphatemia, independently of parathyroid hormone (PTH) and other factors regulating phosphate metabolism. METHODS:We measured serum phosphate levels (sPi), renal tubular reabsorption of Pi (TmPi/G...
journal_title:European journal of endocrinology
pub_type: 杂志文章
doi:10.1530/EJE-10-1150
更新日期:2011-05-01 00:00:00
abstract:OBJECTIVES:Investigation of small numbers of parathyroid tumours by X-chromosome inactivation analysis suggests that the majority of them are monoclonal lesions most likely caused by a somatic mutation. Somatic mutations in the MEN1 gene located on chromosome 11q13 have recently been identified in 12-17% of solitary pa...
journal_title:European journal of endocrinology
pub_type: 杂志文章
doi:10.1530/eje.0.1430047
更新日期:2000-07-01 00:00:00
abstract::The identification and sequencing of the ob gene and its product, leptin, in 1994 opened new insights in the study of the mechanisms controlling body weight and led to a surge of research activity. Since its discovery, leptin has been the subject of an enormous amount of work especially within the fields of nutrition,...
journal_title:European journal of endocrinology
pub_type: 杂志文章,评审
doi:10.1530/eje.0.1490257
更新日期:2003-10-01 00:00:00
abstract::The present study examined the changes in mRNA levels of a pituitary-specific trans-acting factor, Pit-1, and prolactin during the rat estrous cycle. Total cytoplasmic RNA was analyzed by Northern blot and slot-blot hybridization to examine the prolactin mRNA level. Reverse transcription-polymerase chain reaction (RT-...
journal_title:European journal of endocrinology
pub_type: 杂志文章
doi:10.1530/eje.0.1320771
更新日期:1995-06-01 00:00:00
abstract::Design Hypercortisolism during pregnancy is a risk factor for prematurity. Long-term exposure to hypercortisolism may lead to permanent comorbidities, such as hypertension or diabetes, even after remission. Our aim was to determine whether women with a history of Cushing's disease (and being eu-, hypo- or hypercortiso...
journal_title:European journal of endocrinology
pub_type: 杂志文章
doi:10.1530/EJE-20-1224
更新日期:2021-01-01 00:00:00
abstract:OBJECTIVE:The type 2 deiodinase (D2) is a key enzyme for intracellular triiodothyronine (T(3)) generation. A single-nucleotide polymorphism in D2 (Thr92Ala) has been associated with increased insulin resistance in nondiabetic and type 2 diabetes (DM2) subjects. Our aim was to evaluate whether the D2 Thr92Ala polymorphi...
journal_title:European journal of endocrinology
pub_type: 杂志文章,meta分析
doi:10.1530/EJE-10-0419
更新日期:2010-09-01 00:00:00
abstract:BACKGROUND:Leptin, which is the product of the obese gene, is believed to play important roles in pubertal development and reproductive function in females. In a study using adult male rats, it was found that leptin stimulated secretion of gonadotropin from the pituitary in a dose-related manner. However, there has bee...
journal_title:European journal of endocrinology
pub_type: 杂志文章
doi:10.1530/eje.0.1440653
更新日期:2001-06-01 00:00:00
abstract:OBJECTIVE:To establish an Inuit body mass index (BMI) norm from a healthy, not malnourished, pure Inuit population and to investigate the development of overweight in the Inuit in Greenland. DESIGN:Longitudinal study with 35 years follow-up on overweight among Inuit in Greenland. METHODS:The heights and weights of 97...
journal_title:European journal of endocrinology
pub_type: 杂志文章
doi:10.1530/eje.0.1510735
更新日期:2004-12-01 00:00:00
abstract:OBJECTIVE:A phase I single centre, open label study of the pharmacokinetics and tolerability of a buccal testosterone tablet (COL 1621) was carried out. DESIGN:Twelve testosterone-deficient males were treated with the buccal tablet twice daily for 7 consecutive days. Multiple blood samples were drawn for testosterone,...
journal_title:European journal of endocrinology
pub_type: 临床试验,杂志文章
doi:10.1530/eje.0.1500057
更新日期:2004-01-01 00:00:00
abstract:OBJECTIVE:The malignancy of thyroid oncocytic tumours, or oncocytomas, is higher than that of follicular tumours. The aim of this study was to investigate the role of thyroid-specific genes in oncocytic tumours and papillary carcinomas. DESIGN AND METHODS:We compared 29 oncocytic tumours with 12 papillary carcinomas. ...
journal_title:European journal of endocrinology
pub_type: 杂志文章
doi:10.1530/eje.0.1500269
更新日期:2004-03-01 00:00:00
abstract::Regulatory actions of angiotensin II (AngII), which is involved in the pathophysiology of hypertension and also participates in cell proliferation and cell differentiation, are mainly mediated by AngII type 1 (AT1) receptor. Recently, activating mutations of receptors causing hyperfunctioning endocrine diseases have b...
journal_title:European journal of endocrinology
pub_type: 杂志文章
doi:10.1530/eje.0.1370262
更新日期:1997-09-01 00:00:00
abstract::The experimental model of chronic inhibition of nitric oxide (NO) production has proven to be a useful tool to study cardiovascular and renal lesions produced by this type of hypertension, which are similar to those found in human hypertension. It also offers a unique opportunity to study the interaction of NO with th...
journal_title:European journal of endocrinology
pub_type: 杂志文章
doi:10.1530/eje.1.02314
更新日期:2007-01-01 00:00:00
abstract:OBJECTIVE:In order to characterize urinary gonadotropin peptide (UGP) as an acute phase reactant, we focused on the UGP levels after surgical operation. DESIGN:Fifty cases of gastrointestinal cancer, 4 cases of cancers of other organs and 13 cases of benign digestive diseases were enrolled into this study. METHODS:UG...
journal_title:European journal of endocrinology
pub_type: 临床试验,杂志文章
doi:10.1530/eje.0.1400555
更新日期:1999-06-01 00:00:00
abstract::Growth in neuroblastoma cells is regulated by insulin-like growth factors (IGFs) whose action is modulated by IGF binding proteins (IGFBPs). In this study, SK-N-SH neuroblastoma cells were shown to produce IGF-II, IGFBP-2, IGFBP-4 and small quantities of IGFBP-6. We have studied the effects of a natural morphogen, ret...
journal_title:European journal of endocrinology
pub_type: 杂志文章
doi:10.1530/eje.0.1340474
更新日期:1996-04-01 00:00:00
abstract:CONTEXT:Steroidogenic acute regulatory (STAR) gene mutations lead to adrenal and gonadal failure. Interesting, though as yet unexplained, features are the formation of ovarian cysts and the potential presence of CNS findings. OBJECTIVE:To report biochemical, genetic, and long-term clinical data in five Greek patients ...
journal_title:European journal of endocrinology
pub_type: 杂志文章
doi:10.1530/EJE-12-0600
更新日期:2013-02-15 00:00:00
abstract:OBJECTIVE:In view of their different actions on thyroid hormone receptor (TR) isoforms we set out to investigate whether amiodarone (AM) and dronedarone (Dron) have different and/or component-specific effects on cardiac gene expression. DESIGN:Rats were treated with AM or Dron and the expression of TRalpha 1, TRalpha ...
journal_title:European journal of endocrinology
pub_type: 杂志文章
doi:10.1530/EJE-07-0017
更新日期:2007-06-01 00:00:00
abstract:OBJECTIVE:The molecular mechanisms of TSH receptor (TSHR) activation and intramolecular signal transduction are largely unknown. Deletion of the extracellular domain (ECD) of the TSHR results in increased constitutive activity, which suggests a self-inhibitory interaction between the ECD and the extracellular loops (EC...
journal_title:European journal of endocrinology
pub_type: 杂志文章
doi:10.1530/eje.1.01891
更新日期:2005-04-01 00:00:00
abstract:BACKGROUND:Elevated levels of circulating fibroblast growth factor 21 (FGF21) are commonly encountered in type 2 diabetes, dyslipidemia, and non-alcoholic fatty liver disease, all of which share exaggerated postprandial lipemia as a common proatherogenic feature. How FGF21 responds to an oral fat load in man is unknown...
journal_title:European journal of endocrinology
pub_type: 杂志文章
doi:10.1530/EJE-11-0783
更新日期:2012-03-01 00:00:00
abstract:OBJECTIVE:Previous evidence has suggested that a low sex hormone-binding globulin (SHBG) concentration is associated with insulin-resistance and a low adiponectin concentration. We investigated the association between SHBG and the risk of hyperglycemia in each sex and we determined potential interactions between SHBG a...
journal_title:European journal of endocrinology
pub_type: 杂志文章
doi:10.1530/EJE-09-0202
更新日期:2009-07-01 00:00:00
abstract::Nelson's syndrome is a potentially life-threatening condition that does not infrequently develop following total bilateral adrenalectomy (TBA) for the treatment of Cushing's disease. In this review article, we discuss some controversial aspects of Nelson's syndrome including diagnosis, predictive factors, aetiology, p...
journal_title:European journal of endocrinology
pub_type: 杂志文章,评审
doi:10.1530/EJE-10-0466
更新日期:2010-10-01 00:00:00
abstract:OBJECTIVE:It has been suggested that adiponectin regulates plasma free fatty acid (FFA) clearance by stimulating FFA uptake and/or oxidation in muscle. We aimed to determine changes in plasma adiponectin concentration and adiponectin receptor 1 and 2 mRNA expression in skeletal muscle during and after prolonged exercis...
journal_title:European journal of endocrinology
pub_type: 临床试验,杂志文章
doi:10.1530/eje.1.01872
更新日期:2005-03-01 00:00:00
abstract:OBJECTIVE:To investigate the association between birth weight and risk of type 2 diabetes, abdominal obesity and hypertension among Chinese adults. RESEARCH METHODS AND PROCEDURES:Nine hundred and seventy-three individuals from a population-based cross-sectional survey for the prevalence of type 2 diabetes conducted i...
journal_title:European journal of endocrinology
pub_type: 杂志文章
doi:10.1530/eje.1.02265
更新日期:2006-10-01 00:00:00
abstract:OBJECTIVE AND DESIGN:Patients with type 2 diabetes mellitus (T2D) have an increased fracture risk despite a normal or elevated bone mineral density (BMD). The aim of this cross-sectional in vivo study was to assess parameters of peripheral bone microarchitecture, estimated bone strength and bone remodeling in T2D patie...
journal_title:European journal of endocrinology
pub_type: 杂志文章
doi:10.1530/EJE-15-0860
更新日期:2016-02-01 00:00:00
abstract:OBJECTIVE:To detect exogenous recombinant human GH (rhGH) abuse in female athletes. DESIGN:GH-dependent markers were assayed in serum of 100 female athletes (control group) and in a subgroup of nine female subjects treated with rhGH (0.09 IU/kg body weight, 6 days/week for 3 weeks). METHODS:Cut-off values (mean+2 s.d...
journal_title:European journal of endocrinology
pub_type: 杂志文章
doi:10.1530/EJE-08-0884
更新日期:2009-05-01 00:00:00
abstract::The present study focuses on the pathogenesis of increased frequency of large-vessel disease in diabetes. The diabetic arterial wall displays characteristic alterations of the extracellular matrix secreted by arterial smooth-muscle cells. The effects of insulin and growth hormone on the synthesis of sulphate-labelled ...
journal_title:European journal of endocrinology
pub_type: 杂志文章
doi:10.1530/eje.0.1340326
更新日期:1996-03-01 00:00:00