Abstract:
:Measles virus (MV) fusion requires the participation of both the fusion (F) and hemagglutinin (H) glycoproteins. The canine distemper virus fusion protein (CDVF) cannot substitute for the measles virus fusion protein (MVF) in this process. Introduction of restriction enzyme sites into the cDNAs of CDVF and MVF by site-directed mutagenesis facilitated the production of chimeric F proteins which were tested for their capacity to give fusion when coexpressed with MVH. Fusion resulted when the amino-terminal half of the MVF cysteine-rich region was transferred to CDVF.
journal_name
J Viroljournal_title
Journal of virologyauthors
Wild TF,Fayolle J,Beauverger P,Buckland Rdoi
10.1128/JVI.68.11.7546-7548.1994subject
Has Abstractpub_date
1994-11-01 00:00:00pages
7546-8issue
11eissn
0022-538Xissn
1098-5514journal_volume
68pub_type
杂志文章abstract::The retroviral Gag protein is capable of directing the production and release of virus-like particles in the absence of all other viral components. Budding normally occurs after Gag is transported to the plasma membrane by its membrane-targeting and -binding (M) domain. In the Rous sarcoma virus (RSV) Gag protein, the...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.18.8692-8699.2000
更新日期:2000-09-01 00:00:00
abstract::Neurotropic coronavirus induces acute encephalomyelitis and demyelination in mice. Infection of BALB/c (H-2(d)) mice expressing a dominant negative gamma interferon (IFN-gamma) receptor specifically in oligodendrocytes was examined to determine the influence of IFN-gamma signaling on pathogenesis. Inhibition of IFN-ga...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02373-09
更新日期:2010-03-01 00:00:00
abstract::Defining a macrophage-tropic phenotype for HIV-1 to assess a role in pathogenesis is complicated by the fact that HIV-1 isolates vary continuously in their ability to enter monocyte-derived macrophages (MDMs) in vitro, and MDMs vary in their ability to support HIV-1 entry. To overcome these limitations, we identified ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02477-13
更新日期:2014-02-01 00:00:00
abstract::The EFII cis element is a 38-bp sequence at the 5' end of the Rous sarcoma virus long terminal repeat, extending from nucleotides -229 to -192 (with respect to the viral transcription start site), which is recognized by sequence-specific DNA-binding proteins in avian fibroblast nuclear extracts (L. Sealy and R. Chalkl...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.11.6338-6352.1992
更新日期:1992-11-01 00:00:00
abstract::Circulating leukocytes are important in dissemination of cytomegalovirus (CMV) infection in humans. In the mouse model of murine CMV infection (MCMV), it has been shown that infection peaks on days 5 to 7 after experimental infection, when 0.01 to 0.1% of the circulating leukocytes contain viral DNA. In our laboratory...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.10.6305-6311.1994
更新日期:1994-10-01 00:00:00
abstract::To analyze the proposed growth-inhibitory function of wild-type p53, we compared simian virus 40 (SV40) DNA replication in primary rhesus monkey kidney (PRK) cells, which express wild-type p53, and in the established rhesus monkey kidney cell line LLC-MK2, which expresses a mutated p53 that does not complex with large...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.2.886-893.1993
更新日期:1993-02-01 00:00:00
abstract::The ability to manipulate the genomes of herpesviruses is of eminent importance for obtaining insight into gene function and regulation of gene expression of these complex viruses. Here we report the use of in vivo overlap recombination to generate pseudorabies virus mutants. Cotransfection of up to five overlapping c...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.62.6.2191-2195.1988
更新日期:1988-06-01 00:00:00
abstract::To identify retroviral antigenic determinants recognized by CD4+ T helper cells during tumor rejection, we established four noncytolytic, helper-type, CD4+ T-cell clones by limiting dilution cultures of mixed lymphocyte-tumor cultures from mice immune to a Friend virus-induced tumor, FBL-3. Among these, three T helper...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.8.4533-4542.1993
更新日期:1993-08-01 00:00:00
abstract::We have used feline immunodeficiency virus (FIV) protease (PR) as a mutational system to study the molecular basis of substrate-inhibitor specificity for lentivirus PRs, with a focus on human immunodeficiency virus type 1 (HIV-1) PR. Our previous mutagenesis studies demonstrated that discrete substitutions in the acti...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00374-06
更新日期:2006-08-01 00:00:00
abstract::Latency-associated transcript (LAT) promoter deletion mutants of herpes simplex virus type 1 have a reduced capacity to reactivate following adrenergic induction in the rabbit eye model. We have mapped a reactivation phenotype within LAT and describe the construction of recombinants in which poly(A) addition sites hav...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.4.2449-2459.1996
更新日期:1996-04-01 00:00:00
abstract::For human parainfluenza virus type 3 and many other paramyxoviruses, membrane fusion mediated by the fusion protein (F) has a stringent requirement for the presence of the homotypic hemagglutinin-neuraminidase protein (HN). With the goal of gaining further insight into the role of HN in the fusion process, we develope...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.6.3647-3654.2003
更新日期:2003-03-01 00:00:00
abstract::Herpes simplex virus 1 (HSV-1) genomes are associated with the repressive heterochromatic marks H3K9me2/me3 and H3K27me3 during latency. Previous studies have demonstrated that inhibitors of H3K9me2/me3 histone demethylases reduce the ability of HSV-1 to reactivate from latency. Here we demonstrate that GSK-J4, a spec...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03052-14
更新日期:2015-03-01 00:00:00
abstract::Herpes simplex virus 1 (HSV-1) establishes a lifelong latent infection in host peripheral neurons, including the neurons of the trigeminal ganglia (TG). HSV-1 can reactivate from neurons to cause recurrent infection. During latency, the insulator protein CTCF occupies DNA binding sites on the HSV-1 genome, and these s...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00173-18
更新日期:2018-05-14 00:00:00
abstract::We have examined the exposure and conservation of antigenic epitopes on the surface envelope glycoproteins (gp120 and gp41) of 26 intact, native, primary human immunodeficiency virus type 1 (HIV-1) group M virions of clades A to H. For this, 47 monoclonal antibodies (MAbs) derived from HIV-1-infected patients were use...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.15.7096-7107.2000
更新日期:2000-08-01 00:00:00
abstract::We reported previously that the movement protein (MP) of tomato mosaic tobamovirus is phosphorylated, and we proposed that MP phosphorylation is important for viral pathogenesis. Experimental data indicated that phosphorylation enhances the stability of MP in vivo and enables the protein to assume the correct intracel...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.2.1452-1461.2003
更新日期:2003-01-01 00:00:00
abstract::Moloney murine leukemia virus (M-MuLV) is capable of inducing promonocytic leukemia in 50% of adult BALB/c mice that have received peritoneal injections of pristane, but Friend MuLV strain 57 (F-MuLV) is nonleukemogenic under similar conditions. It was shown earlier that these differences could not be mapped to the U3...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.8.5100-5107.1994
更新日期:1994-08-01 00:00:00
abstract::The structure and replication of a cold-adapted, temperature-sensitive (TS) mutant of an Asian (H2N2) influenza virus was compared with that of its wild-type (WT) parent. Viruses were grown in a chicken kidney cell system, and at the nonpermissive temperature of 40 C, production of infectious TS virus was about 100,00...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.12.6.1503-1511.1973
更新日期:1973-12-01 00:00:00
abstract::Simian immunodeficiency virus (SIV) infection of natural hosts is characterized by nonpathogenic chronic viremia, maintenance of gastrointestinal epithelial barrier integrity, and low numbers of target cells. Assessment of cell-associated virus load in T cell subsets in multiple anatomic compartments of chronically SI...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01556-12
更新日期:2012-10-01 00:00:00
abstract::The Cp promoter of Epstein-Barr virus (EBV) directs most transcription of the EBNA genes in lymphoblastoid cell lines. The functions of two control regions in the Cp promoter have been studied by construction of recombinant EBV strains containing specific mutations in these elements. Mutation of the RBP-Jk (CBF1) bind...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.3.1695-1705.1996
更新日期:1996-03-01 00:00:00
abstract::Cytotoxic CD8+ T lymphocytes (CTL) directed against the matrix protein pp65 are major effectors in controlling infection against human cytomegalovirus (HCMV), a persistent virus of the Betaherpesvirus family. We previously suggested that cross-presentation of pp65 by nonpermissive dendritic cells (DCs) could overcome ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.1.142-150.2002
更新日期:2002-01-01 00:00:00
abstract::Although xenotropic murine leukemia viruses cannot productively infect cells of laboratory mice, cells from various wild-derived mice can support replication of these viruses. Although the virus-sensitive wild mice generally lack all or most of the xenotropic proviral genes characteristic of inbred strains, susceptibi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.55.3.690-695.1985
更新日期:1985-09-01 00:00:00
abstract::Data showing that an apoptotic reaction (the exit into the cytoplasm and nucleolytic internucleosomal degradation of chromosomal DNA, compaction and fragmentation of chromatin, cellular shrinkage, and cytoplasmic blebbing) developed in a subline of HeLa-S3 cells upon nonpermissive poliovirus infection with either a gu...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.2.1181-1189.1995
更新日期:1995-02-01 00:00:00
abstract::More often than not, analyses of virus evolution have considered that virus populations are so large that evolution can be explained by purely deterministic models. However, virus populations could have much smaller effective numbers than the huge reported census numbers, and random genetic drift could be important in...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.18.9906-9911.2003
更新日期:2003-09-01 00:00:00
abstract::Previously we have described a stepwise, energy-dependent pathway for human immunodeficiency virus type 1 (HIV-1) capsid assembly in a cell-free system. In this pathway, Gag polypeptides utilize the cellular factor HP68 and assemble into immature capsids by way of assembly intermediates that have defined biochemical c...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.78.4.1645-1656.2004
更新日期:2004-02-01 00:00:00
abstract::A novel phage lambda mutation, called dc10, which interferes with proper lambda head assembly has been isolated and characterized. Phage lambda carrying this mutation is (i) unable to form plaques at 30 or 37 degrees C but does so at 42 degrees C and (ii) unable to form plaques at 42 degrees C on pN-constitutive hosts...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.29.2.782-788.1979
更新日期:1979-02-01 00:00:00
abstract::The Ebola virus glycoprotein mucin-like domain (MLD) is implicated in Ebola virus cell entry and immune evasion. Using cryo-electron tomography of Ebola virus-like particles, we determined a three-dimensional structure for the full-length glycoprotein in a near-native state and compared it to that of a glycoprotein la...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00870-14
更新日期:2014-09-01 00:00:00
abstract::The mature virion of the alphaherpesvirus pseudorabies virus (PrV) contains a minimum of 31 structural proteins which are recruited into the virus particle by a network of protein-protein interactions which is only incompletely understood. We show here that deletion of the tegument protein pUL21 resulted in a drastic ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01801-06
更新日期:2007-01-01 00:00:00
abstract::Termination of mRNA synthesis in vesicular stomatitis virus (VSV), the prototypic rhabdovirus, is controlled by a 13-nucleotide gene end sequence which comprises the conserved tetranucleotide 3'-AUAC-5', the U(7) tract and the intergenic dinucleotide. mRNAs terminated at this sequence possess 100- to 300-nucleotide-lo...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.15.6901-6913.2001
更新日期:2001-08-01 00:00:00
abstract::The human papillomavirus (HPV) E6 oncoproteins recruit the cellular ubiquitin ligase E6AP/UBE3A to target cellular substrates for proteasome-mediated degradation, and one consequence of this activity is the E6 stimulation of E6AP autoubiquitination and degradation. Recent studies identified an autism-linked mutation w...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01390-17
更新日期:2017-10-27 00:00:00
abstract::In some type C retroviruses, translation of the pol gene appears to require translational suppression of the proximal gag amber codon. To identify the region of the viral nucleic acid responsible for synthesis of the pol gene products, a 300-base-pair DNA fragment containing the stop codon from a type C murine virus (...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.62.10.3574-3580.1988
更新日期:1988-10-01 00:00:00