Abstract:
OBJECTIVE:p63RhoGEF, a guanine nucleotide exchange factor, has been reported 'in vitro' as key mediator of the angiotensin II-induced RhoA/Rho kinase activation leading to vasoconstriction and cardiovascular remodeling. We assessed p63RhoGEF gene and protein expression and RhoA/Rho kinase activity in essential hypertensive and Bartter's and Gitelman's syndrome patients, a human model opposite to hypertension; the latter have, in fact, increased plasma angiotensin II, activation of the renin-angiotensin system, yet normotension/hypotension, reduced peripheral resistance and lack of cardiovascular remodeling due to an endogenously blunted angiotensin II type 1 receptor signaling. METHODS:Mononuclear cell p63RhoGEF gene and protein expression and the phosphorylation status of the myosin phosphatase target protein-1 (MYPT-1), marker of Rho kinase activity, were assessed in essential hypertensive patients, Bartter's/Gitelman's patients and healthy individuals by quantitative real-time PCR and western blot. RESULTS:p63RhoGEF mRNA and protein level and MYPT-1 phosphorylation status were higher in hypertensive patients and lower in Bartter's/Gitelman's patients compared with healthy individuals: p63RhoGEF mRNA level: 0.59 ± 0.17 ΔΔCt vs. 0.37 ± 0.17 vs. 0.20 ± 0.19, analysis of variance (ANOVA): P <0.016; p63RhoGEF protein level 1.35 ± 0.14 vs. 1.09 ± 0.05 vs. 0.90 ± 0.09 densitometric units, ANOVA: P <0.0001; MYPT-1: 1.39 ± 0.34 vs. 1.01 ± 0.12 vs. 0.81 ± 0.06, ANOVA: P < 0.0001. p63RhoGEF mRNA was significantly correlated with both SBP and DBP in both hypertensive patients (R = 0.79, P < 0.02 and R = 0.78, P < 0.02) and in Bartter's syndrome/Gitelman's syndrome patients (R = 0.87, P < 0.001 and R = 0.86, P < 0.001), respectively. CONCLUSION:Increased p63RhoGEF mRNA and protein level and Rho kinase activity are shown for the first time in essential hypertensive patients, whereas the opposite was found in Bartter's/Gitelman's patients, a human model opposite to hypertension. These results combined with other 'in-vitro' studies strongly support the crucial importance of p63RhoGEF in Ang II-mediated signaling involved in the regulation of blood pressure and its long-term complications in humans.
journal_name
J Hypertensjournal_title
Journal of hypertensionauthors
Calò LA,Davis PA,Pagnin E,Dal Maso L,Maiolino G,Seccia TM,Pessina AC,Rossi GPdoi
10.1097/HJH.0000000000000075subject
Has Abstractpub_date
2014-02-01 00:00:00pages
331-8issue
2eissn
0263-6352issn
1473-5598journal_volume
32pub_type
杂志文章abstract::An Na+,K+-ATPase inhibitor possessing inhibitory activity against the specific binding of ouabain to Na+,K+-ATPase has been purified from the plasma of acutely saline-infused hogs. The purification was performed by a combination of Amberlite XAD-2 adsorption chromatography and five steps of high-pressure liquid chroma...
journal_title:Journal of hypertension
pub_type: 杂志文章
doi:10.1097/00004872-198704000-00014
更新日期:1987-04-01 00:00:00
abstract::: Hypertension is a growing health concern worldwide. Established hypertension is a causative factor of heart failure, which is characterized by increased vascular resistance and intractable uncontrolled blood pressure. Hypertension and heart failure have multiple causes and complex pathophysiology but cellular immuni...
journal_title:Journal of hypertension
pub_type: 杂志文章
doi:10.1097/HJH.0000000000002456
更新日期:2020-10-01 00:00:00
abstract:BACKGROUND:Increased arterial stiffness is associated with cardiovascular morbidity and mortality among hypertensive patients. OBJECTIVES:To assess the relationship between ambulatory arterial stiffness index (AASI) and subclinical left ventricular (LV) systolic dysfunction assessed by 2-D speckle-tracking echocardiog...
journal_title:Journal of hypertension
pub_type: 杂志文章
doi:10.1097/HJH.0000000000002330
更新日期:2020-05-01 00:00:00
abstract:OBJECTIVE:To explore the mechanisms for augmented neutral endopeptidase inhibitor-induced natriuresis in deoxycorticosterone acetate (DOCA)-salt rats. METHODS:We examined effects of a neutral endopeptidase-inhibitor, candoxatril, on plasma and urinary brain natriuretic peptide (BNP) levels, the influence of a specific...
journal_title:Journal of hypertension
pub_type: 杂志文章
doi:
更新日期:1994-04-01 00:00:00
abstract:OBJECTIVE:To evaluate the mean levels of blood pressure and hypertension (> or = 140 mmHg systolic or > or = 90 mmHg diastolic pressure or on treatment for hypertension) in the adult English population, and to evaluate any changes in the efficacy of hypertension management between 1994 and 2003. DESIGN/METHODS:Cross-s...
journal_title:Journal of hypertension
pub_type: 杂志文章
doi:10.1097/01.hjh.0000226210.95936.bc
更新日期:2006-06-01 00:00:00
abstract:BACKGROUND:Genome-wide scans from Italy and China suggest a hypertension-susceptible locus between D2S2278 (nucleotides 11,245,080 - 11,245,358) and D2S168 (nucleotides 11,467,214 - 11,467,422) on chromosome 2. METHODS:We performed a large association study of polymorphisms in this region with blood pressure modulatio...
journal_title:Journal of hypertension
pub_type: 杂志文章
doi:10.1097/01.hjh.0000166835.70935.3c
更新日期:2005-05-01 00:00:00
abstract:OBJECTIVES:To evaluate the associations of high awake blood pressure (BP), high asleep BP, and nondipping BP, determined by ambulatory BP monitoring (ABPM), with left ventricular hypertrophy (LVH) and geometry. METHODS:Black and white participants (n = 687) in the Coronary Artery Risk Development in Young Adults study...
journal_title:Journal of hypertension
pub_type: 杂志文章
doi:10.1097/HJH.0000000000002221
更新日期:2020-01-01 00:00:00
abstract:BACKGROUND:In sub-Saharan Africa, cardiovascular disease is becoming a leading cause of death, with high blood pressure as number one risk factor. In Nigeria, access and adherence to hypertension care are poor. A pharmacy-based hypertension care model with remote monitoring by cardiologists through mHealth was piloted ...
journal_title:Journal of hypertension
pub_type: 杂志文章
doi:10.1097/HJH.0000000000001877
更新日期:2019-02-01 00:00:00
abstract:OBJECTIVE:We examined the effects of a calcium antagonist and an angiotensin converting enzyme (ACE) inhibitor on contractile and non-contractile protein metabolism and cardiac function in a monocrotaline-induced right ventricular hypertrophy model, in order to define the effects of these drugs on cardiac hypertrophy. ...
journal_title:Journal of hypertension
pub_type: 杂志文章
doi:
更新日期:1994-10-01 00:00:00
abstract:OBJECTIVE:To characterize the in vivo vascular properties of the spontaneously hypertensive rat (SHR) renal vascular bed by examining vascular conductance/resistance responsiveness to vasoactive agents in vivo and determining whether the filtration surface area of glomerular capillaries is reduced. DESIGN AND METHODS:...
journal_title:Journal of hypertension
pub_type: 杂志文章
doi:10.1097/00004872-200108000-00020
更新日期:2001-08-01 00:00:00
abstract:OBJECTIVE:Blood pressure (BP) load, defined as the percentage of abnormally elevated BP readings, is usually provided on the report of ambulatory BP monitoring. However, the usefulness of BP load is still uncertain. In the present study, we examined whether BP load would be associated, independently of BP level, with t...
journal_title:Journal of hypertension
pub_type: 杂志文章
doi:10.1097/HJH.0b013e3283624f9b
更新日期:2013-09-01 00:00:00
abstract:OBJECTIVE AND METHODS:The vasoactive properties of P1,P4-diadenosine tetraphosphate (Ap4A) were studied by measuring the effects of perfusion pressure of a rat isolated perfused kidney. RESULTS:The vasoconstrictive response to Ap4A was mediated to a large extent to a P2X receptor which could be shown by inhibition wit...
journal_title:Journal of hypertension
pub_type: 杂志文章
doi:10.1097/00004872-199816121-00013
更新日期:1998-12-01 00:00:00
abstract::Several myosin isoforms are expressed in smooth muscle, based upon both heavy and light chain polymorphisms. These myosin isoenzymes reveal distinct detachment rate constants, thus regulating the responsiveness of the VSMC contractile apparatus to Ca2+, as well as its energetical and mechanical behavior. ...
journal_title:Journal of hypertension
pub_type: 杂志文章,评审
doi:10.1097/00004872-199205000-00002
更新日期:1992-05-01 00:00:00
abstract:OBJECTIVE:To determine plasma aldosterone concentration (PAC) and plasma renin concentration (PRC) and aldosterone-to-renin ratio (ARR) values in a population attending a Clinic for Cardiovascular Risk Assessment in Children. METHODS:We assessed ARR and associated factors in a cohort of 287 children (137 female, 4-18 ...
journal_title:Journal of hypertension
pub_type: 杂志文章
doi:10.1097/HJH.0000000000001571
更新日期:2018-02-01 00:00:00
abstract:AIM:The aim of this study was to assess the prevalence of ascending aortic dilatation and to evaluate the possible association between proximal aorta dilatation and structural or functional markers of cardiac organ damage in hypertensive patients. BACKGROUND:Dilatation of the sinus of valsalva (SoV) is a common findin...
journal_title:Journal of hypertension
pub_type: 杂志文章,多中心研究
doi:10.1097/HJH.0000000000001844
更新日期:2019-01-01 00:00:00
abstract:OBJECTIVE:To examine the influence of genetic factors on plasma leptin levels. SUBJECTS AND METHODS:We measured plasma leptin levels, body mass index and body fat distribution in healthy young female monozygotic (n = 19) and dizygotic (n = 14) twins. The twin zygosity was verified by determination of short tandem repe...
journal_title:Journal of hypertension
pub_type: 杂志文章
doi:10.1097/00004872-199917010-00005
更新日期:1999-01-01 00:00:00
abstract:BACKGROUND:Childhood hypertension is an established predictor of adult hypertension and organ damage, an underestimated problem in developing countries. The aim of the study was to assess the prevalence of arterial hypertension, as well as the relationship of blood pressure values and weight class and urinary abnormali...
journal_title:Journal of hypertension
pub_type: 杂志文章
doi:10.1097/HJH.0b013e3283407fe3
更新日期:2011-02-01 00:00:00
abstract:OBJECTIVE:Different radiologists may show considerable variation in their interpretation of renal angiograms. We therefore wished to establish the reliability of their interpretation. DESIGN:Assessment of the intra- and inter-observer agreement of the interpretation of renal angiograms. SETTING:Tertiary referral univ...
journal_title:Journal of hypertension
pub_type: 杂志文章
doi:10.1097/00004872-199917120-00011
更新日期:1999-12-01 00:00:00
abstract:OBJECTIVE:To compare the effects of the angiotensin II receptor antagonist candesartan with the angiotensin-converting enzyme inhibitor enalapril on myocardial fibrosis evaluated by echoreflectivity analysis. METHODS:Hypertensive patients (n = 196) with echocardigraphically documented left ventricular hypertrophy were...
journal_title:Journal of hypertension
pub_type: 杂志文章,随机对照试验
doi:10.1097/HJH.0b013e3283232838
更新日期:2009-03-01 00:00:00
abstract:OBJECTIVES:Current evidence demonstrates that both genetic and environmental factors influence blood pressure. The sympathetic nervous system is a key player in blood pressure control and functional genetic variants of the beta-2 adrenergic receptor (B2AR) have been identified and implicated in the pathogenesis of hype...
journal_title:Journal of hypertension
pub_type: 杂志文章
doi:10.1097/01.hjh.0000209983.28735.33
更新日期:2006-03-01 00:00:00
abstract:OBJECTIVES:To describe patterns of hypertension history in patients with various types of end-stage renal disease (ESRD) and in persons with normal kidney function; and to identify risk factors for the diagnosis 'hypertensive ESRD'. DESIGN:A case-control study. SETTING:Population-based. PARTICIPANTS:Patients with ES...
journal_title:Journal of hypertension
pub_type: 杂志文章
doi:10.1097/00004872-199715040-00016
更新日期:1997-04-01 00:00:00
abstract::Elevated large artery stiffness and pulse pressure have emerged as important risk factors for cardiovascular disease. The genders differ in large artery biomechanical properties throughout the lifespan with females displaying higher stiffness than males during the prepubertal years and a dramatic increase after menopa...
journal_title:Journal of hypertension
pub_type: 杂志文章,评审
doi:10.1097/HJH.0b013e328344da5e
更新日期:2011-06-01 00:00:00
abstract:BACKGROUND:Mineralocorticoid receptor antagonists are increasingly used in patients with treatment-resistant hypertension (TRH). There is experimental evidence for blood pressure (BP) independent effects of mineralocorticoid receptor blockade on cardiovascular target organ damage. We hypothesized that low-dose eplereno...
journal_title:Journal of hypertension
pub_type: 杂志文章,随机对照试验
doi:10.1097/HJH.0000000000001264
更新日期:2017-05-01 00:00:00
abstract:BACKGROUND:Left ventricular (LV) remodeling and aortic stiffness have independent predictive value for all causes and cardiovascular mortality. Because elastic properties of the arterial wall vary along the aortic pathway, we hypothesized that local and regional aortic stiffness could differently impact on LV remodelin...
journal_title:Journal of hypertension
pub_type: 杂志文章
doi:10.1097/HJH.0000000000001597
更新日期:2018-03-01 00:00:00
abstract::Atrial natriuretic peptide (ANP) plays a pivotal role in modulation of vascular function and it is also involved in the pathophysiology of several cardiovascular diseases. We provide an updated overview of the current appraisal of ANP vascular effects in both animal models and humans. We describe the physiological imp...
journal_title:Journal of hypertension
pub_type: 杂志文章,评审
doi:10.1097/HJH.0b013e32835ed5eb
更新日期:2013-06-01 00:00:00
abstract:OBJECTIVE:We hypothesized that downregulation of the antioxidant thioredoxin system contributes to oxidative stress in angiotensin II-induced hypertension. As oestrogen may protect against oxidative stress, we also evaluated whether the thioredoxin system, particularly in the heart, is differentially regulated between ...
journal_title:Journal of hypertension
pub_type: 杂志文章
doi:10.1097/HJH.0b013e3280acac60
更新日期:2007-06-01 00:00:00
abstract:OBJECTIVE:We aimed to investigate whether adjustment for frailty influences the relationship of blood pressure with mortality in elderly patients with type 2 diabetes mellitus (T2DM). METHODS:Patients aged 60 years and older (n = 858) were selected from a prospective observational cohort study of primary care patients...
journal_title:Journal of hypertension
pub_type: 杂志文章
doi:10.1097/HJH.0000000000000555
更新日期:2015-06-01 00:00:00
abstract:OBJECTIVE:To gain insight into the mechanisms which contribute to the development of vascular hypertrophy in the spontaneously hypertensive rat (SHR). DESIGN:These experiments were performed under conditions which most closely mimic the growth of smooth muscle in blood vessels, i.e. once cell-cell contact has been ach...
journal_title:Journal of hypertension
pub_type: 杂志文章
doi:
更新日期:1992-07-01 00:00:00
abstract:OBJECTIVES:Treatment modifications--addition, uptitration, switching, and downtitration--are necessary to address issues such as unattained blood pressure goals, adverse drug events, drug cost, or patient dissatisfaction which lead to treatment discontinuation. This study assessed the patterns of treatment modification...
journal_title:Journal of hypertension
pub_type: 杂志文章
doi:10.1097/HJH.0000000000000806
更新日期:2016-03-01 00:00:00
abstract:BACKGROUND:Although the benefit of hypertension treatment is well established in high-risk patients, there is a paucity of evidence regarding the benefit of treatment in patients with uncomplicated hypertension. METHODS:Hypertensive adult patients were selected from the Korea National Health Insurance Sample Cohort in...
journal_title:Journal of hypertension
pub_type: 杂志文章
doi:10.1097/HJH.0000000000001331
更新日期:2017-05-01 00:00:00