Abstract：
:The influence of transforming growth factor beta 1 (TGF-beta 1) and of dexamethasone on fibronectin (FN) synthesis of human renal tubular epithelial cells in culture (TEC) was studied. Cocultivation with TGF-beta 1 increased the steady state level of FN RNA within 24 to 48 hours. By PCR and Northern blotting it was found that the EDA splice variant of FN was preferentially up-regulated. To quantitate FN protein synthesis, cells were cultivated in the presence of [35S]-methionine and FN was isolated from the cell supernatants, and the cell lysates by adsorption to gelatin-sepharose. In TGF-beta 1 treated cells, a small increase of FN in the cell supernatants was seen (1.7-fold), and a more prominent increase in the cell lysates (4.5-fold). The FN content of the extracellular matrix was also increased in TGF-beta 1 treated cells. Most of the de novo synthesized FN was identified as the EDA-variant of FN. As a further stimulus, dexamethasone was used. Again, an increase of FN-specific mRNA was seen as well as an increased FN protein synthesis. The ratio between FN and EDA-FN, however, was not altered when compared to untreated cells. Thus, an increase in EDA-FN synthesis is obviously stimulus dependent.
journal_name
Kidney Intjournal_title
Kidney internationalauthors
Viedt C,Bürger A,Hänsch GMdoi
10.1038/ki.1995.479subject
Has Abstractpub_date
1995-12-01 00:00:00pages
1810-7issue
6eissn
0085-2538issn
1523-1755pii
S0085-2538(15)59251-Xjournal_volume
48pub_type
杂志文章abstract：:Unilateral ureteral obstruction in rabbits leads to an influx of macrophages into the kidney, a proliferation of interstitial cells, and an increase in arachidonic acid metabolism. The role of the macrophage in the metabolic changes of hydronephrosis was investigated by using endotoxin and nitrogen mustard. The in viv...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.1984.127
更新日期：1984-07-01 00:00:00
abstract：:Vitamin D deficiency is common in patients with chronic kidney disease after renal transplantation. Vitamin D, essential for mineral and bone metabolism, also has myriad beneficial autocrine effects on intact immune responses and defense against infection, as well as suppression of malignant changes. Supplementation w...
journal_title：Kidney international
pub_type： 评论,杂志文章,评审
doi：10.1038/ki.2008.492
更新日期：2009-03-01 00:00:00
abstract：:As new components of the renin-angiotensin system (RAS) are elucidated, our understanding of the complexities of their interactions also advances. Previous studies have determined that podocytes possess a local RAS that can generate angiotensin II. Podocytes have also been shown to express angiotensin-converting enzym...
journal_title：Kidney international
pub_type： 评论,杂志文章
doi：10.1038/ki.2012.127
更新日期：2012-08-01 00:00:00
abstract：:Complement factor 5a (C5a) interaction with its receptor (C5aR1) contributes to the pathogenesis of inflammatory diseases, including acute kidney injury. However, its role in chronic inflammation, particularly in pathogen-associated disorders, is largely unknown. Here we tested whether the development of chronic infla...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1016/j.kint.2016.04.023
更新日期：2016-09-01 00:00:00
abstract：:Sodium depletion has a protective effect on target-organ damage in hypertension independent of blood pressure. Here we tested whether chronic dietary sodium restriction may prevent the development of renal alterations associated with insulin resistance by reducing the inflammatory and oxidant state. Rats were fed norm...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.2012.478
更新日期：2013-04-01 00:00:00
abstract：:The presence of nitric oxide (NO) in the kidney has been implicated in the pathogenesis of human glomerulonephritis. However, the exact type of glomerular cells that express NO synthase (NOS) and the NOS isoform involved in the local production of NO has not been identified in the human diseased kidney. We examined th...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1046/j.1523-1755.1998.00907.x
更新日期：1998-06-01 00:00:00
abstract：:One of the challenges of treating patients with glomerulonephritis is to accurately assess disease activity. As renal biopsies are routinely stained for deposits of C3 activation fragments and glomerular C3 deposits are found in most forms of glomerulonephritis, we sought to determine whether a relatively noninvasive ...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.2011.332
更新日期：2012-01-01 00:00:00
abstract：:Problems with vascular access are an important cause of morbidity and mortality in hemodialysis patients. We established a rodent model of arteriovenous fistula by anastomosing the end of a lateral vein to the side of the ventral artery of the rat tail. All operations were technically successful and in all animals the...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.2008.207
更新日期：2008-08-01 00:00:00
abstract：:Transport and metabolism of adenosine were studied in the S1, S2, and S3 segments of the rabbit proximal renal tubule. Isolated segments were perfused in vitro with uniformly labelled 14C-adenosine to measure the lumen-to-bath flux of adenosine. This flux rate was measured by the disappearance of 14C from the luminal ...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.1992.174
更新日期：1992-05-01 00:00:00
abstract：:Impaired olfactory function is a marker of neurologic dysfunction in the uremic state. Retained uremic toxins not adequately cleared with dialysis may undermine the integrity of the olfactory epithelium and olfactory bulb and malign central olfactory processing. The evidence suggests that only renal transplantation, w...
journal_title：Kidney international
pub_type： 评论,社论
doi：10.1038/ki.2011.219
更新日期：2011-10-01 00:00:00
abstract：:Intrauterine growth retardation (IUGR) aggravates the course of acute mesangioproliferative glomerulonephritis (GN) in the rat. Observational studies in children suggest that IUGR may be associated with a severe course of kidney diseases such as IgA nephropathy. We tested the hypothesis that IUGR leads to aggravation ...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/sj.ki.5001966
更新日期：2006-12-01 00:00:00
abstract：:During vasculitis, activation of the kinin system induces inflammation, whereby the kinin B1-receptor is expressed and activated after ligand binding. Additionally, activated blood cells release microvesicles into the circulation. Here we determined whether leukocyte-derived microvesicles bear B1-kinin receptors durin...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1016/j.kint.2016.09.023
更新日期：2017-01-01 00:00:00
abstract：:Acute urinary obstruction causes interstitial inflammation with leukocyte accumulation and the secretion of soluble mediators. Here we show that unilateral ureteral ligation caused a progressive increase in renal F4/80(+) and F4/80(-) dendritic cells, monocytes, neutrophils and T-cells 24-72 h following obstruction. D...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.2008.394
更新日期：2008-11-01 00:00:00
abstract：:Few studies have examined tubular function after subtotal nephrectomy (Nx) and conservative treatments. The effects of 70% and 80% Nx (associated with dietary phosphate restriction in the latter case) on the apical brush border membrane (BBM) enzymes 5'-nucleotidase, gamma glutamyl-transferase and alkaline-phosphatase...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.1997.485
更新日期：1997-12-01 00:00:00
abstract：:Antioxidants have been used as therapies to decrease oxidative stress and improve CVD risk in hemodialysis (HD) patients. A systematic search of the Medline database (search date 30 April 2011) found 56 studies investigating the effects of antioxidant therapies on biomarkers of oxidative stress (53 studies) or clinica...
journal_title：Kidney international
pub_type： 杂志文章,评审
doi：10.1038/ki.2011.341
更新日期：2012-02-01 00:00:00
abstract：BACKGROUND:Variant forms of lysozyme, a ubiquitous bacteriolytic enzyme, are known to lead to hereditary non-neuropathic renal amyloidosis and, so far, three different mutations of the lysozyme gene have been reported. In this study, we report a novel lysozyme variant, Phe57Ile, associated with renal amyloidosis in thr...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1046/j.1523-1755.2003.00904.x
更新日期：2003-05-01 00:00:00
abstract：:We evaluated short- and long-term effects of high-dose recombinant human erythropoietin (rHuEPO) in kidney transplantation in a prospective double-blind, placebo-controlled study. Patients with chronic kidney disease following receipt of a deceased donor kidney allograft were randomized to 3 doses of 40,000 units rHuE...
journal_title：Kidney international
pub_type： 杂志文章,随机对照试验
doi：10.1038/ki.2011.349
更新日期：2012-02-01 00:00:00
abstract：:We studied in 32 patients on maintenance hemodialysis (duration of treatment 6 to 133 months) whether duration of dialysis treatment affects blood pressure, plasma noradrenaline levels and alpha 2-adrenoceptor density (assessed in platelet membranes by 3H-yohimbine binding). Plasma noradrenaline levels were a signific...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.1987.267
更新日期：1987-11-01 00:00:00
abstract：:A substantial body of evidence has accumulated linking an increased incidence of cardiovascular disease in patients with acute kidney injury (AKI), chronic kidney disease (CKD), and end-stage renal disease (ESRD). A multitude of novel risk factors related to decreased kidney function might interact with the renal and ...
journal_title：Kidney international
pub_type： 杂志文章,评审
doi：10.1038/ki.2011.178
更新日期：2011-09-01 00:00:00
abstract：:We investigated the effect of the potent immunosuppressive agent, FK506, on experimental glomerular thrombosis in rats by combined injections of nephrotoxic serum (NTS) and lipopolysaccharide (LPS). Either FK506 or placebo was administered intramuscularly three hours prior to injection of NTS that was followed one hou...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.1994.207
更新日期：1994-06-01 00:00:00
abstract：:The members of the matrix metalloproteinase gene family play critical roles in numerous physiologic events, including cellular migration, tissue remodeling in wound healing and development, as well as in the evolution of the inflammatory process. The 72 kDa gelatinase A (formerly denoted 72 kDa Type IV collagenase) is...
journal_title：Kidney international
pub_type： 杂志文章,评审
doi：10.1038/ki.1997.191
更新日期：1997-05-01 00:00:00
abstract：:Cystinuria is a common inherited aminoaciduria that leads to recurrent cystine nephrolithiasis. Mutations in a gene encoding a renal amino acid transporter (SLC3A1) have been identified in patients with cystinuria establishing one molecular cause for the disease. To facilitate systematic screening of this gene for mut...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.1997.258
更新日期：1997-06-01 00:00:00
abstract：BACKGROUND:Bone marrow reconstitution using genetically-modified hematopoietic stem cells has been reported to confer resistance to inflammation and prevent renal injury in glomerulonephritis. Although this strategy has potentials for clinical use, taking hematopoietic stem cells from bone marrow is highly stressful fo...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1046/j.1523-1755.2003.00046.x
更新日期：2003-07-01 00:00:00
abstract：:The acutely injured mammalian kidney mounts a cellular and molecular response to repair itself. However, in patchy regions such intrinsic processes are impaired and dysregulated leading to chronic kidney disease. Currently, no therapy exists to treat established acute kidney injury per se. Strategies to augment human ...
journal_title：Kidney international
pub_type： 杂志文章,评审
doi：10.1016/j.kint.2017.07.030
更新日期：2018-01-01 00:00:00
abstract：:Lysyl oxidase like-2 (LOXL2) is an amine oxidase with both intracellular and extracellular functions. Extracellularly, LOXL2 promotes collagen and elastin crosslinking, whereas intracellularly, LOXL2 has been reported to modify histone H3, stabilize SNAIL, and reduce cell polarity. Although LOXL2 promotes liver and lu...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1016/j.kint.2018.02.024
更新日期：2018-08-01 00:00:00
abstract：BACKGROUND:The podocyte is the focus of much research into the mechanisms of renal disease progression, and the number of podocytes per glomerulus has thus become a parameter of much interest. When counting podocytes, the actual particle counted is the cell nucleus. The majority of published studies estimating podocyte...
journal_title：Kidney international
pub_type： 临床试验,杂志文章
doi：10.1111/j.1523-1755.2004.00787.x
更新日期：2004-08-01 00:00:00
abstract：BACKGROUND:We have recently found evidence for increased reactive oxygen species (ROS) in rats with lead-induced hypertension. We hypothesized that increased ROS activity may contribute to hypertension by enhancing inactivation of nitric oxide (NO) in this model. METHODS:Rats were treated for 12 weeks with either lead...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1046/j.1523-1755.1999.00670.x
更新日期：1999-10-01 00:00:00
abstract：:Current practice recommends metabolic evaluation of patients who have formed multiple renal stones, but not those with one stone or temporally remote stones. This presumes that recentness and recurrence imply greater risk of new future stones. We hypothesize that number of stones reflects how long patients are permitt...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.1994.224
更新日期：1994-06-01 00:00:00
abstract：:Patients with chronic renal failure frequently have hypogonadism. To elucidate the molecular mechanisms involved, we tested the ability of serum from these patients to inhibit recombinant human luteinizing hormone receptors. Using a cell line expressing functional human luteinizing hormone receptors, we found that ade...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.1997.109
更新日期：1997-03-01 00:00:00
abstract：:Recent studies suggest that postnatal neovascularization relies not exclusively on sprouting of preexisting vessels ("angiogenesis"), but also involves the contribution of bone marrow-derived circulating endothelial progenitor cells (EPCs). EPCs can be isolated from peripheral blood or bone marrow mononuclear cells, C...
journal_title：Kidney international
pub_type： 杂志文章,评审
doi：10.1111/j.1523-1755.2005.00261.x
更新日期：2005-05-01 00:00:00
