Properties of a polynucleotide synthesized by strain 74A of Neurospora crassa.

Abstract:

:A polynucleotide (or a fragment of RNA) was purified to apparent homogeneity by HPLC from mycelium of the wild strain 74A of the mould Neurospora crassa, after growth on sucrose and in the presence of saturating amounts of inorganic phosphate (Pi) for 72 hr at 30 degrees. The M(r) was ca 20,000 as determined by HPLC at pH 6.8. Polynucleotide synthesis ranged from 4.0 to 6.5 micrograms polynucleotide per mg dry mycelium in mycelium of the wild strain 74A and the various phosphorus regulatory and structural mutant strains of the mould N. crassa. Kinetic data showed that the polynucleotide interacts with mycelial Pi-repressible alkaline phosphatase by inhibiting its p-nitrophenylphosphatase activity and by protecting the enzyme against thermal inactivation in the presence of high concentrations of ammonium sulphate.

journal_name

Phytochemistry

journal_title

Phytochemistry

authors

Carmona EC,Han SW,Palma MS,Rossi A

doi

10.1016/0031-9422(95)00531-5

subject

Has Abstract

pub_date

1996-02-01 00:00:00

pages

345-8

issue

2

eissn

0031-9422

issn

1873-3700

pii

0031-9422(95)00531-5

journal_volume

41

pub_type

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