Abstract:
:A polynucleotide (or a fragment of RNA) was purified to apparent homogeneity by HPLC from mycelium of the wild strain 74A of the mould Neurospora crassa, after growth on sucrose and in the presence of saturating amounts of inorganic phosphate (Pi) for 72 hr at 30 degrees. The M(r) was ca 20,000 as determined by HPLC at pH 6.8. Polynucleotide synthesis ranged from 4.0 to 6.5 micrograms polynucleotide per mg dry mycelium in mycelium of the wild strain 74A and the various phosphorus regulatory and structural mutant strains of the mould N. crassa. Kinetic data showed that the polynucleotide interacts with mycelial Pi-repressible alkaline phosphatase by inhibiting its p-nitrophenylphosphatase activity and by protecting the enzyme against thermal inactivation in the presence of high concentrations of ammonium sulphate.
journal_name
Phytochemistryjournal_title
Phytochemistryauthors
Carmona EC,Han SW,Palma MS,Rossi Adoi
10.1016/0031-9422(95)00531-5subject
Has Abstractpub_date
1996-02-01 00:00:00pages
345-8issue
2eissn
0031-9422issn
1873-3700pii
0031-9422(95)00531-5journal_volume
41pub_type
杂志文章相关文献
PHYTOCHEMISTRY文献大全abstract::Twenty phenolic compounds, representatives of proanthocyanidins and gallic acid/hexahydroxyldiphenic acid esters of glucose, have been assessed for their ability to inhibit binding of specific radioligands to 16 receptors. The receptors utilized were alpha 1-, alpha 2- and beta-adrenoceptors, adenosine 1, dopamine 1 a...
journal_title:Phytochemistry
pub_type: 杂志文章
doi:10.1016/s0031-9422(96)00598-5
更新日期:1997-02-01 00:00:00
abstract::Five p-terphenyl derivatives named thelephantins D-H (1-5) together with nine known compounds, thelephantins A-C (6-8), ganbajunin E (9), p-hydroxylbenzoic acid (10), ganbajunin C (11), thelephorin A (12), 2-O-methylatromentin (13) and atromentin (14), were isolated from the methanolic extract of fruit bodies of the T...
journal_title:Phytochemistry
pub_type: 杂志文章
doi:10.1016/s0031-9422(03)00220-6
更新日期:2003-08-01 00:00:00
abstract::Benzoxazinoids are secondary metabolites that are effective in defence and allelopathy. They are synthesised in two subfamilies of the Poaceae and sporadically found in single species of the dicots. The biosynthesis is fully elucidated in maize; here the genes encoding the enzymes of the pathway are in physical proxim...
journal_title:Phytochemistry
pub_type: 杂志文章,评审
doi:10.1016/j.phytochem.2009.05.012
更新日期:2009-10-01 00:00:00
abstract::Preparative-scale fermentation of papaveraldine (1), the known benzylisoquinoline alkaloid, with Mucor ramannianus 1839 (sih) has resulted in a stereoselective reduction of the ketone group and the isolation of S-papaverinol (2) and S-papaverinol N-oxide (3). The structure elucidation of both metabolites was based pri...
journal_title:Phytochemistry
pub_type: 杂志文章
doi:10.1016/s0031-9422(99)00616-0
更新日期:2000-03-01 00:00:00
abstract::The structure of four diterpenoid glucosides, designated as salvigresides A-D (1-4), isolated from the aerial parts of Salvia greggii, have been confirmed by spectroscopic and chemical investigation. ...
journal_title:Phytochemistry
pub_type: 杂志文章
doi:10.1016/j.phytochem.2004.08.012
更新日期:2004-09-01 00:00:00
abstract::Seventeen undescribed heterodimers of meroterpene phenol, psocorylins A-Q, were isolated from the fruits of Psoralea corylifolia. Their another monomeric unit derived from flavonone, chalcone, coumarin and isoflavone, respectively. Psocorylins A-E were rare natural spiroketals with the skeleton of 1,4,8-trioxaspiro[4....
journal_title:Phytochemistry
pub_type: 杂志文章
doi:10.1016/j.phytochem.2020.112394
更新日期:2020-08-01 00:00:00
abstract::Tetraoxygenated xanthones, cowaxanthones A-E, together with 10 previously reported tetraoxygenated xanthones, were isolated from the crude hexane extract of the fruits of Garcinia cowa. Cowaxanthone B has previously been reported as a synthetic xanthone. Their structures were elucidated by analysis of spectroscopic da...
journal_title:Phytochemistry
pub_type: 杂志文章
doi:10.1016/j.phytochem.2006.02.027
更新日期:2006-05-01 00:00:00
abstract::Blueberry consumption is associated with health benefits contributing to a reduced risk for cardiovascular disease, diabetes and cancer. The aim of this study was to determine the anthocyanin profile of blueberry extracts and to evaluate their effects on B16-F10 metastatic melanoma murine cells. Seven blueberry cultiv...
journal_title:Phytochemistry
pub_type: 杂志文章
doi:10.1016/j.phytochem.2013.06.018
更新日期:2013-11-01 00:00:00
abstract::Germacrene D is considered as a precursor of many sesquiterpene hydrocarbons. We have investigated the acid catalyzed as well as the photochemically and thermally induced rearrangement processes of germacrene D isolated from several Solidago species, which contain both enantiomers of germacrene D. Enantiomeric mixture...
journal_title:Phytochemistry
pub_type: 杂志文章
doi:10.1016/s0031-9422(00)00266-1
更新日期:2000-09-01 00:00:00
abstract::Two novel triterpenoid saponins, dianchinenoside C [23-O-beta-D-glucopyranosyl 3 beta, 16 alpha-dihydroxyolean-12-ene 23 alpha, 28 beta-dioic acid 28-O-beta-D-glucopyranoside] and dianchinenoside D [3 beta, 16 alpha-dihydroxyolean-12-ene 23 alpha, 28 beta-dioic acid 28-O-beta-D-glucopyranosyl [1-->6)-beta-D-glucopyran...
journal_title:Phytochemistry
pub_type: 杂志文章
doi:10.1016/s0031-9422(00)90599-5
更新日期:1994-02-01 00:00:00
abstract::Activity guided fractionations led to the isolation of two antitumor compounds 5 alpha,8 alpha-epidioxy-24(R)-methylcholesta-6,22-dien-3 beta-D-glucopyranoside and 5,6-epoxy-24(R)-methylcholesta-7,22-dien-3 beta-ol from the methanol extract of Cordyceps sinensis. Two previously known compounds, ergosteryl-3-O-beta-D-g...
journal_title:Phytochemistry
pub_type: 杂志文章
doi:10.1016/s0031-9422(99)00128-4
更新日期:1999-08-01 00:00:00
abstract::Two cell wall proteins from chickpea, known to be rapidly insolubilised by an elicitor-stimulated oxidative burst in-vivo, were purified from suspension cells. N-terminal protein sequencing revealed them as a proline-rich protein and an extensin-like protein. Oxidative cross-linking could be modelled in an in vitro sy...
journal_title:Phytochemistry
pub_type: 杂志文章
doi:10.1016/s0031-9422(99)00463-x
更新日期:2000-01-01 00:00:00
abstract::The Madagascar periwinkle produces a large palette of Monoterpenoid Indole Alkaloids (MIAs), a class of complex alkaloids including some of the most valuable plant natural products with precious therapeutical values. Evolutionary pressure on one of the hotspots of biodiversity has obviously turned this endemic Malagas...
journal_title:Phytochemistry
pub_type: 杂志文章,评审
doi:10.1016/j.phytochem.2014.07.023
更新日期:2015-05-01 00:00:00
abstract::The tetrahydroanthraquinones (1S,3S)-austrocortilutein (4) and (1S,3S)-austrocortirubin (7) are octaketides formed in the fruit bodies of the fungus Dermocybe splendida via the gentiobioside 2 of the dihydroanthracenone (S)-torosachrysone (1). ...
journal_title:Phytochemistry
pub_type: 杂志文章
doi:10.1016/s0031-9422(00)00181-3
更新日期:2000-09-01 00:00:00
abstract::The challenges and opportunities for protecting agricultural production of food and other materials will be met through exploiting the induction of defence pathways in plants to control pests, diseases and weeds. These approaches will involve processes that can be activated by application of natural products, patented...
journal_title:Phytochemistry
pub_type: 杂志文章,评审
doi:10.1016/j.phytochem.2007.09.025
更新日期:2007-11-01 00:00:00
abstract::Fomannoxin is a biologically active benzohydrofuran, which has been suggested to be involved in the pathogenicity of the root rotting fungus Heterobasidion annosum sensu lato. The biosynthesis of fomannoxin was investigated through an isotopic enrichment study utilizing [1-¹³C]glucose as metabolic tracer. ¹³C NMR spec...
journal_title:Phytochemistry
pub_type: 杂志文章
doi:10.1016/j.phytochem.2012.08.008
更新日期:2012-12-01 00:00:00
abstract::Chemical systematics sets out to interpret the phylogenetic implications of the occurrence and distribution of secondary metabolites. In this review, a number of the major contributions from the 1960's and 1970's are identified and re-assessed in the light of recent evidence gained from DNA studies. It is shown that f...
journal_title:Phytochemistry
pub_type: 杂志文章,评审
doi:10.1016/j.phytochem.2007.06.029
更新日期:2007-11-01 00:00:00
abstract::The patterns of secondary metabolites in leaves of yeast invertase-transgenic tobacco plants (Nicotiana tabacum L. cv. Samsun NN) were analyzed. Plants expressing cytosolic yeast-derived invertase (cytInv) or apoplastic (cell wall associated) yeast invertase (cwInv) showed a characteristic phytochemical phenotype comp...
journal_title:Phytochemistry
pub_type: 杂志文章
doi:10.1016/s0031-9422(00)00422-2
更新日期:2001-03-01 00:00:00
abstract::Myrothecium verrucaria was found to be an effective pathogen against kudzu grown in the greenhouse and the field. M. verrucaria produced large amounts of macrocyclic trichothecenes when cultured on solid rice medium, including epiroridin E (16.8 mg/g crude extract), epiisororidin E (1 mg/g), roridin E (8.7 mg/g), rori...
journal_title:Phytochemistry
pub_type: 杂志文章
doi:10.1016/s0031-9422(01)00214-x
更新日期:2001-09-01 00:00:00
abstract::Indole alkaloids, oxytrofalcatins A-F (1-6), together with five other known alkaloids (7-11), were isolated from the roots of Oxytropis falcata. Their structures were elucidated by comprehensive spectroscopic analyses, including using 1D and 2D NMR spectroscopy and mass spectrometry. This is the first report of N-benz...
journal_title:Phytochemistry
pub_type: 杂志文章
doi:10.1016/j.phytochem.2010.02.010
更新日期:2010-06-01 00:00:00
abstract::A flavonoid trioside and its coumaryl ester together with seven known flavonoids and five phenolic acids were isolated from the leaves of Reseda muricata. Their structures were elucidated by spectroscopic methods including UV, FAB MS, 1H, 13C and 2D-NMR, DEPT, HMBC and HMQC experiments. The two compounds were identifi...
journal_title:Phytochemistry
pub_type: 杂志文章
doi:10.1016/s0031-9422(00)00479-9
更新日期:2001-06-01 00:00:00
abstract::A bioassay-guided fractionation of Agave offoyana leaves led to the isolation of five steroidal saponins (1-5) along with six known saponins (6-11). The compounds were identified as (25R)-spirost-5-en-2α,3β-diol-12-one 3-O-{α-l-rhamnopyranosyl-(1→3)-O-β-d-glucopyranosyl-(1→2)-O-[β-d-xylopyranosyl-(1→3)]-O-β-d-glucopyr...
journal_title:Phytochemistry
pub_type: 杂志文章
doi:10.1016/j.phytochem.2014.05.014
更新日期:2014-09-01 00:00:00
abstract::Papain (EC 3.4.22.2), the archetypal cysteine protease of C1 family, is of considerable commercial significance. In order to obtain substantial quantities of active papain, the DNA coding for propapain, the papain precursor, has been cloned and expressed at a high level in Escherichia coli BL21(DE3) transformed with t...
journal_title:Phytochemistry
pub_type: 杂志文章
doi:10.1016/j.phytochem.2009.02.001
更新日期:2009-03-01 00:00:00
abstract::Lathyrus sativus (grass pea) is a high-yielding, drought-resistant legume consumed as a food in Northern India and neighboring countries as well as in Ethiopia. Its development into an important food legume, however, has been hindered by the presence of the neurotoxin - beta-N-oxalyl-L-alpha,beta-diaminopropionic acid...
journal_title:Phytochemistry
pub_type: 杂志文章,评审
doi:10.1016/j.phytochem.2005.10.022
更新日期:2006-01-01 00:00:00
abstract::Chemical investigation of the vines and leaves of Momordica charantia resulted in isolation of fourteen cucurbitane triterpenoids, kuguacins F-S (1-14), including two pentanorcucurbitacins (6 and 7), one octanorcucurbitacin (8), and two trinorcucurbitacins (11 and 12), along with six known analogues. Their structures ...
journal_title:Phytochemistry
pub_type: 杂志文章
doi:10.1016/j.phytochem.2008.10.011
更新日期:2009-01-01 00:00:00
abstract::A lignan diglycoside, (-)-isolariciresinol 3alpha-O-beta-apiofuranosyl-(1-->2)-O-beta-glucopyranoside, and a megastigmane glucoside, sauroposide, were isolated from the aerial part of Sauropus androgynus together with (+)-isolariciresinol 3alpha-O-beta-glucopyranoside, (-)-isolariciresinol 3alpha-O-beta-glucopyranosid...
journal_title:Phytochemistry
pub_type: 杂志文章
doi:10.1016/s0031-9422(03)00219-x
更新日期:2003-08-01 00:00:00
abstract::The leaf essential oils of Cistus monspeliensis plants growing wild on calcareous and siliceous soils in Provence (South of France) were analysed by GC and GC-MS. Qualitative and quantitative differences are noted in the essential oil composition between the Cistus populations from the two soil types. For chemotaxonom...
journal_title:Phytochemistry
pub_type: 杂志文章
doi:10.1016/s0031-9422(99)00460-4
更新日期:2000-01-01 00:00:00
abstract::ent-19-Hydroxykaur-16-en-15-one is transformed to ent-3 beta,16 beta,19-trihydroxykauran-15-one by Cephalosporium aphidicola. The structure of this metabolite was established by X-ray crystallography. ...
journal_title:Phytochemistry
pub_type: 杂志文章
doi:10.1016/0031-9422(94)85065-8
更新日期:1994-09-01 00:00:00
abstract::The incomplete structure proposed in 1972 for a unique "flavone-polysaccharide" compound, MF-1, from the liverwort Monoclea forsteri, has been re-examined. Rather than the proposed 8-methoxyluteolin structure with polysaccharides attached to the 7- and 4'-hydroxyls, MF-1 has been shown to be primarily a mixture of pen...
journal_title:Phytochemistry
pub_type: 杂志文章
doi:10.1016/s0031-9422(03)00199-7
更新日期:2003-07-01 00:00:00
abstract::Marine sponges are an unusually rich source of bioactive natural products with clinical potential. They also often harbor rich communities of symbiotic bacteria that have often been suspected as the true producers of sponge-derived compounds. To date, these bacteria can in most cases not be cultivated, but culture-ind...
journal_title:Phytochemistry
pub_type: 杂志文章,评审
doi:10.1016/j.phytochem.2009.04.010
更新日期:2009-10-01 00:00:00