当前位置： SCI文献检索 > TRANSACTIONS OF THE ROYAL SOCIETY OF TROPICAL MEDICINE AND HYGIENE期刊下所有文献 > Detection of malaria in Malaysia by nested polymerase chain reaction amplification of dried blood spots on filter papers.

Detection of malaria in Malaysia by nested polymerase chain reaction amplification of dried blood spots on filter papers.

Abstract：

:A modified nested polymerase chain reaction (PCR) method for detection of Plasmodium falciparum, P. vivax and P. malariae was combined with a simple blood collection and deoxyribonucleic acid (DNA) extraction method and evaluated in Malaysia. Finger-prick blood samples from 46 hospital patients and 120 individuals living in malaria endemic areas were spotted on filter papers and dried. The simple Chelex method was used to prepare DNA templates for the nested PCR assay. Higher malaria prevalence rates for both clinical (78.2%) and field samples (30.8%) were obtained with the nested PCR method than by microscopy (76.1% and 27.5%, respectively). Nested PCR was more sensitive than microscopy in detecting mixed P. falciparum and P. vivax infections, detected 5 more malaria samples than microscopy on the first round of microscopical examination, and detected malaria in 3 microscopically negative samples. Nested PCR failed to detect parasite DNA in 2 microscopically positive samples, an overall sensitivity of 97.4% compared to microscopy. The nested PCR method, when coupled with simple dried blood spot sampling, is a useful tool for collecting accurate malaria epidemiological data, particularly in remote regions of the world.

journal_name

Trans R Soc Trop Med Hyg

journal_title

Transactions of the Royal Society of Tropical Medicine and Hygiene

authors

Singh B,Cox-Singh J,Miller AO,Abdullah MS,Snounou G,Rahman HA

doi

10.1016/s0035-9203(96)90302-8

subject

Has Abstract

pub_date

1996-09-01 00:00:00

pages

519-21

issue

5

eissn

0035-9203

issn

1878-3503

journal_volume

90

pub_type

杂志文章

相关文献

TRANSACTIONS OF THE ROYAL SOCIETY OF TROPICAL MEDICINE AND HYGIENE文献大全
  • Epidemic Schistosoma mansoni in a known S. haematobium area.

    abstract：:An epidemic of bloody diarrhoea was observed in 1997 and 1998 in Bessoum, north Cameroon. Of 1176 inhabitants, 16.7% went for medical consultation to a public health centre located 10 km away. This epidemic was probably due to the recent introduction of Schistosoma mansoni in an ancient focus of S. haematobium, follow...

    journal_title：Transactions of the Royal Society of Tropical Medicine and Hygiene

    pub_type： 杂志文章

    doi：10.1016/s0035-9203(00)90221-9

    authors： Cunin P,Griffet A,Poste B,Djibrilla K,Martin PM

    更新日期：2000-11-01 00:00:00

  • The epidemiology of myelopathy associated with human T-lymphotropic virus 1.

    abstract：:A progressive spastic myelopathy is one of the principal manifestations of the human T cell lymphotropic virus type 1. Recent research is reviewed on the geography and epidemiology of this specific form of tropical spastic paraparesis. First recognized in the Caribbean, Colombia and Japan, it is now also confirmed as ...

    journal_title：Transactions of the Royal Society of Tropical Medicine and Hygiene

    pub_type： 杂志文章,评审

    doi：10.1016/0035-9203(93)90467-5

    authors： Montgomery RD

    更新日期：1993-03-01 00:00:00

  • The eye in cerebral malaria: what can it teach us?

    abstract：:The pathophysiology of coma in cerebral malaria (CM) is not well understood. Obstruction of microcirculatory flow is thought to play a central role, but other hypotheses include roles for parasite- and host-derived factors such as immune mediators, and for increased blood-brain barrier permeability leading to raised i...

    journal_title：Transactions of the Royal Society of Tropical Medicine and Hygiene

    pub_type： 杂志文章,评审

    doi：10.1016/j.trstmh.2008.11.003

    authors： Maude RJ,Dondorp AM,Abu Sayeed A,Day NP,White NJ,Beare NA

    更新日期：2009-07-01 00:00:00

  • Oliva vidua fulminans, a marine mollusc, responsible for five fatal cases of neurotoxic food poisoning in Sabah, Malaysia.

    abstract：:This is the first report in which a marine mollusc, Oliva vidua fulminans (olives), generally not known to be poisonous, was responsible for death in five children after consuming boiled olives with tamarind. The onset of symptoms was rapid 10 to 20 min after consumption of the olives. Signs and symptoms included naus...

    journal_title：Transactions of the Royal Society of Tropical Medicine and Hygiene

    pub_type： 杂志文章

    doi：10.1016/0035-9203(86)90197-5

    authors： Kan SK,Singh N,Chan MK

    更新日期：1986-01-01 00:00:00

  • Wuchereria bancrofti infection in human and mosquito populations of a Polynesian village ten years after interruption of mass chemoprophylaxis with diethylcarbamazine.

    abstract：:In 1991, a study on Wuchereria bancrofti microfilariae (mf) and infection rates was carried out in the human and mosquito populations of a Polynesian village where, 10 years before, the mf prevalence rate was 6.4% and twice-yearly mass treatment with 3 mg/kg of diethylcarbamazine (DEC) was interrupted. Venous blood sa...

    journal_title：Transactions of the Royal Society of Tropical Medicine and Hygiene

    pub_type： 杂志文章

    doi：10.1016/0035-9203(92)90245-8

    authors： Cartel JL,Nguyen NL,Spiegel A,Moulia-Pelat JP,Plichart R,Martin PM,Manuellan AB,Lardeux F

    更新日期：1992-07-01 00:00:00

  • Vectorborne diseases in West Africa: geographic distribution and geospatial characteristics.

    abstract：:This paper provides an overview of the methods in which geographic information systems (GIS) and remote sensing (RS) technology have been used to visualise and analyse data related to vectorborne diseases (VBD) in West Africa and to discuss the potential for these approaches to be routinely included in future studies ...

    journal_title：Transactions of the Royal Society of Tropical Medicine and Hygiene

    pub_type： 杂志文章,评审

    doi：10.1093/trstmh/trt020

    authors： Ratmanov P,Mediannikov O,Raoult D

    更新日期：2013-05-01 00:00:00

  • Immunity to malaria in young Gambian children after a two-year period of chemoprophylaxis.

    abstract：:A cohort of 48 Gambian children was protected against malaria by fortnightly administration of Maloprim (pyrimethamine and dapsone) for 2 years between their 3 and 5 birthdays. A matched cohort of 47 children received placebo. During the year following the termination of prophylaxis there was no increase in the freque...

    journal_title：Transactions of the Royal Society of Tropical Medicine and Hygiene

    pub_type： 临床试验,杂志文章

    doi：10.1016/0035-9203(88)90263-5

    authors： Otoo LN,Snow RW,Menon A,Byass P,Greenwood BM

    更新日期：1988-01-01 00:00:00

  • Urban yellow fever epidemic in western Nigeria, 1987.

    abstract：:A large epidemic of urban yellow fever occurred in April and May 1987 in Oyo State, western Nigeria. The principal vector was Aedes aegypti, breeding in domestic water containers. The 1987 outbreak followed an epidemic of sylvatic yellow fever in eastern Nigeria the previous year, and probably resulted from introducti...

    journal_title：Transactions of the Royal Society of Tropical Medicine and Hygiene

    pub_type： 杂志文章

    doi：10.1016/0035-9203(89)90518-x

    authors： Nasidi A,Monath TP,DeCock K,Tomori O,Cordellier R,Olaleye OD,Harry TO,Adeniyi JA,Sorungbe AO,Ajose-Coker AO

    更新日期：1989-05-01 00:00:00

  • Sequential imaging studies of cerebral gnathostomiasis with subdural hemorrhage as its complication.

    abstract：:Cerebral gnathostomiasis is a severe form of human infection caused by Gnathostoma spinigerum. We report sequential brain imaging in serologically proven cerebral gnathostomiasis. The clinical and radiographic correlation showed the probable route taken by the parasite during its migration through the central nervous ...

    journal_title：Transactions of the Royal Society of Tropical Medicine and Hygiene

    pub_type： 杂志文章

    doi：10.1016/j.trstmh.2008.09.011

    authors： Sawanyawisuth K,Chlebicki MP,Pratt E,Kanpittaya J,Intapan PM

    更新日期：2009-01-01 00:00:00

  • The survival in nature of Wuchereia-infected Anopheles funestus Giles in North-eastern Tanzania.

    abstract：:Hungry Anopheles funestus females were caught in CDC light traps positioned overnight in village houses in which the occupants were protected by bed nets. The sampling was performed in six huts near Muheza, in North-eastern Tanzania, September to November 1972. Parous, unfed mosquitoes were carefully examined, usually...

    journal_title：Transactions of the Royal Society of Tropical Medicine and Hygiene

    pub_type： 杂志文章

    doi：10.1016/0035-9203(77)90086-4

    authors： Krafsur ES,Garrett-Jones C

    更新日期：1977-01-01 00:00:00

  • The atypical lymphocyte count: a novel predictive factor for severe thrombocytopenia related to dengue.

    abstract：BACKGROUND:Early identification of patients at risk of severe dengue disease (DD) is critical to guide its management. We evaluated whether the atypical lymphocyte count (ALC), generated from the Sysmex automated full blood count analyzer, is predictive of severe thrombocytopenia secondary to Dengue infection. METHODS...

    journal_title：Transactions of the Royal Society of Tropical Medicine and Hygiene

    pub_type： 杂志文章

    doi：10.1093/trstmh/trz128

    authors： Abeysuriya V,Choong CSH,Thilakawardana BU,de Mel P,Shalindi M,de Mel C,Chandrasena L,Seneviratne SL,Yip C,Yap ES,de Mel S

    更新日期：2020-06-01 00:00:00

  • Malaria epidemiology and detectability.

    abstract：:Conventional malaria surveys based on the microscopic examination of blood do not always detect chronic, low-grade infections caused by immunity. A model incorporating differences in detectability within the host population shows how the false negative tests caused by poor detectability confound measures of prevalence...

    journal_title：Transactions of the Royal Society of Tropical Medicine and Hygiene

    pub_type： 杂志文章

    doi：10.1016/0035-9203(82)90219-x

    authors： Aron JL

    更新日期：1982-01-01 00:00:00

  • Blood pressures of South African Black adolescents aged 16 to 17 years.

    abstract：:In South Africa, in urban but not rural areas, hypertension is far more common in Black than in White adults. To throw light on this adverse situation, blood pressures are being determined in various segments of Black populations, in the present instance on series of school pupils aged 16 to 17 years, in rural and urb...

    journal_title：Transactions of the Royal Society of Tropical Medicine and Hygiene

    pub_type： 杂志文章

    doi：10.1016/0035-9203(80)90147-9

    authors： Walker AR,Walker BF,Daya L,Ncongwane J

    更新日期：1980-01-01 00:00:00

  • Dengue epidemic in the State of Rio Grande do Norte, Brazil, in 1997.

    abstract：:During 1997 a large dengue epidemic occurred in Rio Grande do Norte, a State in north-east Brazil. The co-circulation of dengue virus type 1 and dengue virus type 2 was demonstrated by virus isolation in Aedes albopictus clone C6/36 cell-line and by reverse transcriptase-polymerase chain reaction (RT-PCR). IgM capture...

    journal_title：Transactions of the Royal Society of Tropical Medicine and Hygiene

    pub_type： 杂志文章

    doi：10.1016/s0035-9203(99)90008-1

    authors： Cunha RV,Schatzmayr HG,Miagostovich MP,Barbosa AM,Paiva FG,Miranda RM,Ramos CC,Coelho JC,dos Santos FB,Nogueira RM

    更新日期：1999-05-01 00:00:00

  • Herbal toxicity in Zimbabwe.

    abstract：:Indigenous natural drugs are in common use in Zimbabwe because modern life-saving drugs are beyond the reach of nearly 85% of the population. These natural drugs have caused a number of poisoning cases. In a study of the records of four hospitals from 1971 to 1982, carried out to see how many people had been poisoned ...

    journal_title：Transactions of the Royal Society of Tropical Medicine and Hygiene

    pub_type： 杂志文章

    doi：10.1016/0035-9203(86)90339-1

    authors： Nyazema NZ

    更新日期：1986-01-01 00:00:00

  • Public health impact of establishing the cause of bacterial infections in rural Asia.

    abstract：:Recent studies delineating bacterial causes of fever in rural Asia indicate a major role for several previously under-recognized pathogens, including Rickettsia and Leptospira. The use of blood culture for the first time to investigate patients with febrile illness in rural Asia has also revealed some unexpected findi...

    journal_title：Transactions of the Royal Society of Tropical Medicine and Hygiene

    pub_type： 杂志文章,评审

    doi：10.1016/j.trstmh.2007.06.004

    authors： Peacock SJ,Newton PN

    更新日期：2008-01-01 00:00:00

  • Imported filariasis in Pakistan.

    abstract：:Night blood surveys for filariasis were carried out in two camps of repatriates from Bangladesh. The sample consisted of 1,101 Biharis above one year of age of whom 9.0% were found infected with Wuchereria bancrofti. The infection rate was significantly higher in males (10.2%) than in females (6.7%). Of the three mosq...

    journal_title：Transactions of the Royal Society of Tropical Medicine and Hygiene

    pub_type： 杂志文章

    doi：10.1016/0035-9203(81)90435-1

    authors： Aslamkhan M,Pervez SD

    更新日期：1981-01-01 00:00:00

  • The superiority of the miniature Anion-Exchange Centrifugation Technique for detecting low grade trypanosome parasitaemias.

    abstract：:The efficiency was tested of the Haematocrit Centrifugation Technique (HCT) and the miniature Anion-Exchange Centrifugation Technique (mAECT) for demonstrating trypanosomes in the blood of four antelopes experimentally infected with Trypanosoma brucei gambiense in Liberia. During simultaneous daily application of both...

    journal_title：Transactions of the Royal Society of Tropical Medicine and Hygiene

    pub_type： 杂志文章

    doi：10.1016/0035-9203(84)90244-x

    authors： Sachs R

    更新日期：1984-01-01 00:00:00

  • Isolation of Trichophyton concentricum from chronic cutaneous lesions in patients from the Solomon Islands.

    abstract：:Tinea imbricata, also known as 'Tokelau', is an uncommon superficial mycosis caused by the anthropophilic dermatophyte Trichophyton concentricum. Cutaneous lesions appear characteristically as scaly and concentric rings that may cover all parts of the body. Often acquired in childhood, tinea imbricata is a chronic dis...

    journal_title：Transactions of the Royal Society of Tropical Medicine and Hygiene

    pub_type： 杂志文章

    doi：10.1016/j.trstmh.2008.01.002

    authors： Pihet M,Bourgeois H,Mazière JY,Berlioz-Arthaud A,Bouchara JP,Chabasse D

    更新日期：2008-04-01 00:00:00

  • Skin disorders and disease profile of poverty: analysis of medical records in Tigray, northern Ethiopia, 2005-2007.

    abstract：:Skin disease is among the most frequent causes of morbidity in Ethiopia, showing high prevalence in the general population and being the sixth most frequent cause of outpatient visits nationwide to health facilities. This study was aimed at describing skin disease patterns in Tigray region, northern Ethiopia through a...

    journal_title：Transactions of the Royal Society of Tropical Medicine and Hygiene

    pub_type： 杂志文章

    doi：10.1016/j.trstmh.2008.11.028

    authors： Accorsi S,Barnabas GA,Farese P,Padovese V,Terranova M,Racalbuto V,Morrone A

    更新日期：2009-05-01 00:00:00

  • Growth and diarrhoeal disease surveillance in a rural Ghanaian pre-school child population.

    abstract：:This paper is a report of the first year of follow-up on 250 pre-school children in a village in Ghana. The mean weight for age and the mean height for age at the start of the study, ranged between 81 and 97% and 91 and 101.4% of the median WHO reference standards respectively. Classified according to Waterlow (1973) ...

    journal_title：Transactions of the Royal Society of Tropical Medicine and Hygiene

    pub_type： 杂志文章

    doi：10.1016/0035-9203(86)90012-x

    authors： Biritwum RB,Isomura S,Assoku A,Torigoe S

    更新日期：1986-01-01 00:00:00

  • Mebendazole in parasitic infections other than those caused by soil-transmitted helminths.

    abstract：:Mebendazole, a benzimidazole carbamate compound, is currently in use for human medical practice against soil-transmitted helminthiasis (STH) and enterobiasis. However, it has been demonstrated that its spectrum of activity is broad and goes beyond those infections. Several studies provide evidence that this drug, take...

    journal_title：Transactions of the Royal Society of Tropical Medicine and Hygiene

    pub_type： 杂志文章,评审

    doi：10.1016/j.trstmh.2008.11.029

    authors： Cañete R,Escobedo AA,Almirall P,González ME,Brito K,Cimerman S

    更新日期：2009-05-01 00:00:00

  • Aerosol immunisation for TB: matching route of vaccination to route of infection.

    abstract：:TB remains a very significant global health burden. There is an urgent need for better tools for TB control, which include an effective vaccine. Bacillus Calmette-Guérin (BCG), the currently licensed vaccine, confers highly variable protection against pulmonary TB, the main source of TB transmission. Replacing BCG com...

    journal_title：Transactions of the Royal Society of Tropical Medicine and Hygiene

    pub_type： 杂志文章,评审

    doi：10.1093/trstmh/tru206

    authors： Manjaly Thomas ZR,McShane H

    更新日期：2015-03-01 00:00:00

  • Incidence of localized cutaneous leishmaniasis (chiclero's ulcer) in Mexico.

    abstract：:Cutaneous leishmaniasis in Mexico was first described in 1912. Since then, its existence has been repeatedly confirmed in the forest regions of the peninsula of Yucatan. In order to obtain reliable knowledge about the magnitude of this problem, we questioned and skin-tested a sample of 449 persons randomly selected fr...

    journal_title：Transactions of the Royal Society of Tropical Medicine and Hygiene

    pub_type： 杂志文章

    doi：10.1016/0035-9203(90)90262-d

    authors： Andrade-Narváez FJ,Simmonds-Díaz E,Rico-Aguilar S,Andrade-Narvéez M,Palomo-Cetina A,Canto-Lara SB,García-Miss MR,Madera-Sevilla M,Albertos-Alpuche N

    更新日期：1990-03-01 00:00:00

  • The lethal effects of the cibarial and pharyngeal armatures of mosquitoes on microfilariae.

    abstract：:Microfilariae of Wuchereria bancrofti and Brugia pahangi were killed by the chewing action of the cibarial and pharyngeal armatures and other papillae and spines in the fore-gut of mosquitoes. The proportion of ingested microfilariae that were killed was largely dependent on the presence and shape of the cibarial arma...

    journal_title：Transactions of the Royal Society of Tropical Medicine and Hygiene

    pub_type： 杂志文章

    doi：10.1016/0035-9203(78)90128-1

    authors： McGreevy PB,Bryan JH,Oothuman P,Kolstrup N

    更新日期：1978-01-01 00:00:00

  • Achromobacter xylosoxidans resistance to antiseptics and disinfectants is far from obvious.

    abstract：BACKGROUND:Achromobacter xylosoxidans is described as being resistant to antiseptics and disinfectants. We studied in vitro the ability of five strains to survive and grow in such solutions, with and without starvation. METHODS:Bacterial suspensions in rich media and in distilled water were inoculated into eight antis...

    journal_title：Transactions of the Royal Society of Tropical Medicine and Hygiene

    pub_type： 杂志文章

    doi：10.1093/trstmh/trz016

    authors： Marion-Sanchez K,Pailla K,Cesarine M,Platon MG,Derancourt C,Olive C

    更新日期：2019-06-01 00:00:00

  • Dengue mortality: reassessing the risks in transition countries.

    abstract：:In Singapore in 2005 there were 14,209 documented cases of dengue fever, including 25 deaths. The epidemiology of dengue in Singapore is changing, with increasingly severe infection in adults with chronic disease being recognised. We performed a retrospective review of nine adult patients who died of dengue-related il...

    journal_title：Transactions of the Royal Society of Tropical Medicine and Hygiene

    pub_type： 杂志文章

    doi：10.1016/j.trstmh.2008.06.005

    authors： Lahiri M,Fisher D,Tambyah PA

    更新日期：2008-10-01 00:00:00

  • Malaria chemoprophylaxis, birth weight and child survival.

    abstract：:Study of the effects of malaria chemoprophylaxis given during pregnancy on birthweight and investigation of the influence of birthweight on child survival suggest that, in a rural area of The Gambia, chemoprophylaxis given during pregnancy might reduce infant mortality by about one-fifth in the children of primigravid...

    journal_title：Transactions of the Royal Society of Tropical Medicine and Hygiene

    pub_type： 临床试验,杂志文章,随机对照试验

    doi：10.1016/0035-9203(92)90078-q

    authors： Greenwood AM,Armstrong JR,Byass P,Snow RW,Greenwood BM

    更新日期：1992-09-01 00:00:00

  • Systematic review of risk factors for nonadherence to TB treatment in immigrant populations.

    abstract：BACKGROUND:Foreign-born populations carry a significant TB burden in low-prevalence countries, composing over half of all cases in parts of Europe and North America. This study systematically reviewed evidence of risk factors for nonadherence to TB drug therapy in this group. METHODS:On 28 October 2013 MEDLINE, CINAHL...

    journal_title：Transactions of the Royal Society of Tropical Medicine and Hygiene

    pub_type： 杂志文章,评审

    doi：10.1093/trstmh/trw025

    authors： Lin S,Melendez-Torres GJ

    更新日期：2016-05-01 00:00:00

  • Profile distribution of juvenile Aedes species in an urban area of Malaysia.

    abstract：BACKGROUND:Dengue fever (DF) is an urban vector-borne disease transmitted by Aedes aegypti and Aedes albopictus. Both species deposit their eggs in favorable breeding sites either in natural or artificial containers. An understanding of their habitat characteristics is crucial in curbing DF outbreaks METHODS:A search ...

    journal_title：Transactions of the Royal Society of Tropical Medicine and Hygiene

    pub_type： 杂志文章

    doi：10.1093/trstmh/trw015

    authors： Dom NC,Faiz Madzlan M,Nadira Yusoff SN,Hassan Ahmad A,Ismail R,Nazrina Camalxaman S

    更新日期：2016-04-01 00:00:00