Abstract：
:We have shown that the PC12 cell line is an excellent model system for investigations of the molecular and cellular processes involved in O2-chemosensitivity. We have identified an O2-sensitive K channel in this cell line that mediates membrane depolarization, an increase in intracellular free Ca2+, and dopamine release during hypoxia. We also presented evidence which shows that expression of the gene for tyrosine hydroxylase, the rate-limiting enzyme in dopamine biosynthesis, is stimulated by reduced O2 tension in PC12 and type I carotid body cells. In addition, we have successfully identified the DNA sequences and trans-acting protein factors that regulate transcription of the TH gene during hypoxia. The mechanisms by which a reduction in O2 tension is transduced into alter cell function including increased gene expression remain unknown. Unpublished results from our laboratory show that the increased TH gene expression during hypoxia does not require activation of the cAMP-PKA signal transduction pathway. We propose that the increase in intracellular free Ca2+ that occurs as a result of membrane depolarization might play an important role. Preliminary findings from our laboratory show that blockade of the voltage operated Ca2+ channel or chelation of intracellular Ca2+ prevent full activation of the TH gene during hypoxia.
journal_name
Adv Exp Med Bioljournal_title
Advances in experimental medicine and biologyauthors
Millhorn DE,Conforti L,Beitner-Johnson D,Zhu W,Raymond R,Filisko T,Kobayashi S,Peng M,Genter MBdoi
10.1007/978-1-4615-5891-0_20subject
Has Abstractpub_date
1996-01-01 00:00:00pages
135-42eissn
0065-2598issn
2214-8019journal_volume
410pub_type
杂志文章,评审abstract：:Epidemiologic evidence in humans and controlled trials in animal models indicate that total dietary fat increases the risk of cancer. The animal evidence indicates that the greatest efficacy in promoting carcinogenesis is achieved with omega-6 fatty acids with little or no effect from either the omega-3 or monounsatur...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章,评审
doi：10.1007/978-1-4613-1151-5_7
更新日期：1996-01-01 00:00:00
abstract：:Neurons are long-lived post-mitotic cells that possess an elaborate system of endosomes and lysosomes (endolysosomes) for protein quality control. Relatively recently, endolysosomes were recognized to contain high concentrations (400-600 μM) of readily releasable calcium. The release of calcium from this acidic organe...
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更新日期：2020-01-01 00:00:00
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更新日期：1998-01-01 00:00:00
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更新日期：2007-01-01 00:00:00
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更新日期：2020-01-01 00:00:00
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更新日期：1993-01-01 00:00:00
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更新日期：2013-01-01 00:00:00
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更新日期：2015-01-01 00:00:00
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更新日期：2018-01-01 00:00:00
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更新日期：2010-01-01 00:00:00
