Abstract:
:The gH-gL complex of herpes simplex virus type 1 (HSV-1) is essential for virion infectivity and virus-induced cell fusion, but functional domains of the gH molecule remain to be defined. We have addressed this question by mutagenesis. A set of linker insertion mutants in HSV-1 gH was generated and tested in transient assays for their ability to complement a gH-negative virus. Insertions at three sites in the C-terminal third of the external domain affected the ability of gH to function in cell-cell fusion and virus entry, while insertions at six sites in the N-terminal half of the external domain induced conformational changes in gH such that it was not recognized by monoclonal antibody LP11, although expression at the cell surface was unchanged. A recombinant virus in which a potential integrin-binding motif, RGD, in gH was changed to the triplet RGE entered cells as efficiently as the wild type, indicating that HSV-1 entry is not mediated by means of the gH-RGD motif binding to cell surface integrins. Furthermore, mutagenesis of the glycosylation site which is positionally conserved in all herpesvirus gH sequences in close proximity to the transmembrane domain generated a recombinant virus that grew in vitro with wild-type single-step kinetics.
journal_name
J Viroljournal_title
Journal of virologyauthors
Galdiero M,Whiteley A,Bruun B,Bell S,Minson T,Browne Hdoi
10.1128/JVI.71.3.2163-2170.1997subject
Has Abstractpub_date
1997-03-01 00:00:00pages
2163-70issue
3eissn
0022-538Xissn
1098-5514journal_volume
71pub_type
杂志文章abstract::The ultrastructure of CV-1 cells infected with subacute sclerosing panencephalitis (SSPE) viruses was compared with that of CV-1 cells infected with the wild or Edmonston strain of measles virus. Both SSPE viruses and the measles viruses produced two types of nucleocapsid structures: smooth filaments, 15 to 17 nm in d...
journal_title:Journal of virology
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.62.8.2636-2643.1988
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doi:10.1128/JVI.66.8.4784-4793.1992
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.22.13848-13855.2005
更新日期:2005-11-01 00:00:00
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pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.9.4390-4398.1990
更新日期:1990-09-01 00:00:00
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journal_title:Journal of virology
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journal_title:Journal of virology
pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:1999-05-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/JVI.32.1.175-186.1979
更新日期:1979-10-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.4.1727-1734.1991
更新日期:1991-04-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.18.2.481-490.1976
更新日期:1976-05-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00761-17
更新日期:2017-09-12 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.1.151-157.1998
更新日期:1998-01-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.58.2.331-338.1986
更新日期:1986-05-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/JVI.64.4.1517-1522.1990
更新日期:1990-04-01 00:00:00
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journal_title:Journal of virology
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journal_title:Journal of virology
pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.15.4.954-963.1975
更新日期:1975-04-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.15.3.534-539.1975
更新日期:1975-03-01 00:00:00
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pub_type: 杂志文章
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更新日期:2003-07-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:1991-08-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.10.5266-5270.2002
更新日期:2002-05-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2003-02-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.49.1.57-65.1984
更新日期:1984-01-01 00:00:00
abstract:UNLABELLED:Ebola virus (EBOV) belongs to the group of nonsegmented negative-sense RNA viruses. The seven EBOV genes are separated by variable gene borders, including short (4- or 5-nucleotide) intergenic regions (IRs), a single long (144-nucleotide) IR, and gene overlaps, where the neighboring gene end and start signal...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01863-14
更新日期:2014-11-01 00:00:00