Abstract:
:A procedure is described for the large-scale production of pure suspensions of human lymphocytes and granulocytes. Platelet-poor buffy-coats, obtained by centrifugation of six units of ABO-compatible ACD blood, were pooled and centrifuged again, resulting in a crude leukocyte concentrate containing approximately 60 per cent of the original amount of leukocytes. Further fractionation of such crude leukocyte concentrates was achieved by scaling up Bøyum's Ficoll-Isopaque density gradient technique with the aid of an IBM 2991 blood cell processor. In this way 2.9 +/- 0.3 X 10(9) mononuclear leukocytes, consisting of 90 +/- 0.7 per cent lymphocytes and 5.3 +/- 0.3 X 10(9) polymorphonuclear leukocytes of 92 +/- 0.7 per cent purity could be isolated in one run. The overall yield was 36 per cent of the original amount of leukocytes.
journal_name
Transfusionjournal_title
Transfusionauthors
de la Rivière AB,Verhoef-Karssen PR,de Wit JJ,Loos JA,Prins HKdoi
10.1046/j.1537-2995.1977.17578014593.xsubject
Has Abstractpub_date
1977-09-01 00:00:00pages
509-12issue
5eissn
0041-1132issn
1537-2995journal_volume
17pub_type
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