当前位置： SCI文献检索 > BRITISH JOURNAL OF CANCER期刊下所有文献 > Putative tumour suppressor gene necdin is hypermethylated and mutated in human cancer.

Putative tumour suppressor gene necdin is hypermethylated and mutated in human cancer.

Abstract：

BACKGROUND:Necdin (NDN) expression is downregulated in telomerase-immortalised normal human urothelial cells. Telomerase-immortalised normal human urothelial cells have no detected genetic alterations. Accordingly, many of the genes whose expression is altered following immortalisation are those for which epigenetic silencing is reported. METHODS:NDN expression was examined in normal tissues and tumour cell lines by quantitative real-time PCR and immunoblotting. Immunohistochemistry was performed on urothelial carcinoma (UC). Urothelial carcinoma and UC cell lines were subject to HumanMethylation27 BeadChip Array-based methylation analyses. Mutation screening was performed. The functional significance of NDN expression was investigated using retroviral-mediated downregulation or overexpression. RESULTS:NDN protein was widely expressed in normal tissues. Loss of expression was observed in 38 out of 44 (86%) of UC cell lines and 19 out of 25 (76%) of non-UC cell lines. Loss of NDN protein was found in the majority of primary UC. Oncomine analysis demonstrated downregulation of expression in multiple tumour types. In UC, tumour-specific hypermethylation of NDN and key CpG sites where hypermethylation correlated with reduced expression were identified. Six novel mutations, including some of predicted functional significance, were identified in colorectal and ovarian cancer cell lines. Functional studies showed that NDN could suppress colony formation at low cell density and affect anchorage-independent growth and anoikis in vitro. CONCLUSION:NDN is a novel tumour suppressor candidate that is downregulated and hypermethylated or mutated in cancer.

journal_name

Br J Cancer

journal_title

British journal of cancer

authors

De Faveri LE,Hurst CD,Platt FM,Taylor CF,Roulson JA,Sanchez-Carbayo M,Knowles MA,Chapman EJ

doi

10.1038/bjc.2013.104

subject

Has Abstract

pub_date

2013-04-02 00:00:00

pages

1368-77

issue

6

eissn

0007-0920

issn

1532-1827

pii

bjc2013104

journal_volume

108

pub_type

杂志文章

相关文献

BRITISH JOURNAL OF CANCER文献大全
  • Underexpression of peroxisome proliferator-activated receptor (PPAR)gamma in PAX8/PPARgamma-negative thyroid tumours.

    abstract：:The expression of peroxisome proliferator-activated receptor (PPAR)gamma in thyroid neoplasias and in normal thyroid (NT) tissues has not been fully investigated. The objectives of the present work were: to study and compare the relative expression of PPARgamma in normal, benign and malignant thyroid tissues and to co...

    journal_title：British journal of cancer

    pub_type： 杂志文章

    doi：10.1038/sj.bjc.6601989

    authors： Marques AR,Espadinha C,Frias MJ,Roque L,Catarino AL,Sobrinho LG,Leite V

    更新日期：2004-08-16 00:00:00

  • Expression of 32/67-kDa laminin receptor in laminin adhesion-selected human colon cancer cell lines.

    abstract：:Laminin promotes the malignant phenotype, and the expression of certain laminin receptors is increased in malignancy. Previously, we demonstrated that a laminin-adhesive subclone of a human colon cancer cell line showed increased tumorigenicity in nude mice and increased affinity of the beta1 integrin for laminin rela...

    journal_title：British journal of cancer

    pub_type： 杂志文章

    doi：10.1038/bjc.1998.3

    authors： Kim WH,Lee BL,Jun SH,Song SY,Kleinman HK

    更新日期：1998-01-01 00:00:00

  • Comparison of prognostic factors in patients in phase I trials of cytotoxic drugs vs new noncytotoxic agents.

    abstract：:The aims of this study were to identify prognostic variables for toxicity and survival in patients with cancer participating in phase I clinical trials and compare characteristics of those treated with cytotoxic chemotherapy (CT) and non-cytotoxic drugs (non-CT). Data were collected from 420 (114 CT, 306 non-CT) patie...

    journal_title：British journal of cancer

    pub_type： 杂志文章

    doi：10.1038/sj.bjc.6601218

    authors： Han C,Braybrooke JP,Deplanque G,Taylor M,Mackintosh D,Kaur K,Samouri K,Ganesan TS,Harris AL,Talbot DC

    更新日期：2003-10-06 00:00:00

  • Combining DNA damaging therapeutics with immunotherapy: more haste, less speed.

    abstract：:The idea that chemotherapy can be used in combination with immunotherapy may seem somewhat counterproductive, as it can theoretically eliminate the immune cells needed for antitumour immunity. However, much preclinical work has now demonstrated that in addition to direct cytotoxic effects on cancer cells, a proportion...

    journal_title：British journal of cancer

    pub_type： 杂志文章,评审

    doi：10.1038/bjc.2017.376

    authors： Brown JS,Sundar R,Lopez J

    更新日期：2018-02-06 00:00:00

  • Drug sensitivity testing on patient-derived sarcoma cells predicts patient response to treatment and identifies c-Sarc inhibitors as active drugs for translocation sarcomas.

    abstract：BACKGROUND:Heterogeneity and low incidence comprise the biggest challenge in sarcoma diagnosis and treatment. Chemotherapy, although efficient for some sarcoma subtypes, generally results in poor clinical responses and is mostly recommended for advanced disease. Specific genomic aberrations have been identified in some...

    journal_title：British journal of cancer

    pub_type： 杂志文章

    doi：10.1038/s41416-018-0359-4

    authors： Brodin BA,Wennerberg K,Lidbrink E,Brosjö O,Potdar S,Wilson JN,Ma L,Moens LN,Hesla A,Porovic E,Bernhardsson E,Papakonstantinou A,Bauer H,Tsagkozis P,von Sivers K,Wejde J,Östling P,Kallioniemi O,Stragliotto CL

    更新日期：2019-02-01 00:00:00

  • Involvement of chromosome 6 in endometrial cancer.

    abstract：:Cytogenetic investigation was performed on direct preparations of 15 endometrial cancers showing different histotypes. Clonal abnormalities were found in 11 out of 13 analysable cases. The modal chromosome number was near diploid in all cases. The abnormal karyotypes contained relatively simple numerical or structural...

    journal_title：British journal of cancer

    pub_type： 杂志文章

    doi：10.1038/bjc.1997.312

    authors： Tibiletti MG,Bernasconi B,Taborelli M,Furlan D,Fabbri A,Franchi M,Taramelli R,Trubia M,Capella C

    更新日期：1997-01-01 00:00:00

  • Suramin inhibits bFGF-induced endothelial cell proliferation and angiogenesis in the chick chorioallantoic membrane.

    abstract：:The effects of suramin, an inhibitor of growth factor mitogenic activity, were evaluated on basic fibroblast growth factor (bFGF)-induced proliferation of bovine aortic endothelial cells and on angiogenesis in the chorioallantoic membrane (CAM) of chick embryos. The role of bFGF gene expression in endothelial cell gro...

    journal_title：British journal of cancer

    pub_type： 杂志文章

    doi：10.1038/bjc.1993.457

    authors： Danesi R,Del Bianchi S,Soldani P,Campagni A,La Rocca RV,Myers CE,Paparelli A,Del Tacca M

    更新日期：1993-11-01 00:00:00

  • Syngeneic immune response to rat tracheal epithelial cells transformed in vitro by N-methyl-N-nitro-N-nitrosoguanidine.

    abstract：:Two cell lines (2-10-1 and 8-10-2) derived by exposure to primary tracheal explants to MNNG in vitro were not tumorigenic in syngeneic F-344 rats or athymic BALB/c (nu/nu) mice at early passage, but became tumorigenic at late passage. These cell lines are therefore suited to study the expression of neoantigens during ...

    journal_title：British journal of cancer

    pub_type： 杂志文章

    doi：10.1038/bjc.1981.176

    authors： Braslawsky GR,Steele V,Kennel SJ,Nettesheim P

    更新日期：1981-08-01 00:00:00

  • Influence of menstrual cycle, parity and oral contraceptive use on steroid hormone receptors in normal breast.

    abstract：:Steroid receptor was assessed immunohistochemically in 158 samples of normal breast for variation through the menstrual cycle. Patterns and intensity of reaction were used in a semi-quantitative scoring system to examine the influence of cycle phase, cycle type, parity and age. The changes in oestrogen receptor for na...

    journal_title：British journal of cancer

    pub_type： 杂志文章

    doi：10.1038/bjc.1992.122

    authors： Battersby S,Robertson BJ,Anderson TJ,King RJ,McPherson K

    更新日期：1992-04-01 00:00:00

  • Aerosolised 5-azacytidine suppresses tumour growth and reprogrammes the epigenome in an orthotopic lung cancer model.

    abstract：BACKGROUND:Epigenetic silencing by promoter methylation and chromatin remodelling affects hundreds of genes and is a causal event for lung cancer. Treatment of patients with low doses of the demethylating agent 5-azacytidine in combination with the histone deacetylase inhibitor entinostat has yielded clinical responses...

    journal_title：British journal of cancer

    pub_type： 杂志文章

    doi：10.1038/bjc.2013.575

    authors： Reed MD,Tellez CS,Grimes MJ,Picchi MA,Tessema M,Cheng YS,March TH,Kuehl PJ,Belinsky SA

    更新日期：2013-10-01 00:00:00

  • Serum CA125 level is a good prognostic indicator in lung cancer.

    abstract：:The serum CA125 level was determined by a one-step immunoradiometric assay method in patients with lung cancer. Increased serum CA125 levels were observed in 37.8% of patients with squamous cell cancer, in 30.0% of those with adenocarcinoma and in 60.0% of those with small call cancer. Most patients with increased ser...

    journal_title：British journal of cancer

    pub_type： 杂志文章

    doi：10.1038/bjc.1990.355

    authors： Kimura Y,Fujii T,Hamamoto K,Miyagawa N,Kataoka M,Iio A

    更新日期：1990-10-01 00:00:00

  • Role of reactive oxygen species in cis-dichlorodiammineplatinum-induced cytotoxicity on bladder cancer cells.

    abstract：:This study was undertaken to investigate the intracellular induction of reactive oxygen species (ROS) by cis-dichlorodiammineplatinum (CDDP) and the augmentation of their cytotoxicity in bladder cancer cells (KU7) by enhancement of ROS generation by the glutathione (GSH) depletors buthionine sulphoximine (BSO) and die...

    journal_title：British journal of cancer

    pub_type： 杂志文章

    doi：10.1038/bjc.1997.363

    authors： Miyajima A,Nakashima J,Yoshioka K,Tachibana M,Tazaki H,Murai M

    更新日期：1997-01-01 00:00:00

  • K20 and ICO-10 monoclonal antibodies (gp120/200; Thy-1): immunophenotyping of human solid tumours.

    abstract：:Solid tumour cells were shown to express VLA-beta and Thy-1 antigens. For identification of these molecules two monoclonal antibodies, K-20 and ICO-10, characterised in detail previously, were used. Four groups of solid tumours have been identified according to their immunophenotype: VLA-beta+ and Thy-1-; VLA-beta+ an...

    journal_title：British journal of cancer

    pub_type： 杂志文章

    doi：10.1038/bjc.1990.39

    authors： Kadagidze ZG,Tupitsyn NN,Baryshnikov AJ,Kadyrov KP,Blinov VM,Bernard A,Amiot M,Boumsell L

    更新日期：1990-02-01 00:00:00

  • Specific, reversible G1 arrest by UCN-01 in vivo provides cytostatic protection of normal cells against cytotoxic chemotherapy in breast cancer.

    abstract：BACKGROUND:Low-dose UCN-01 mediates G1 arrest in normal proliferating cell lines with an intact G1 to S transition but not tumour cells with a deregulated G1 to S checkpoint. Here we hypothesised that UCN-01 is effective in mediating a selective, reversible G1 arrest of normal proliferating cells, resulting in decrease...

    journal_title：British journal of cancer

    pub_type： 杂志文章

    doi：10.1038/s41416-019-0707-z

    authors： Mull BB,Livingston JA,Patel N,Bui T,Hunt KK,Keyomarsi K

    更新日期：2020-03-01 00:00:00

  • Immunostaining of thymidylate synthase and p53 for predicting chemoresistance to S-1/cisplatin in gastric cancer.

    abstract：:High expression of thymidylate synthase (TS) and inactivation of p53 are allegedly associated with chemoresistance. The authors evaluated TS and p53 expression in gastric cancer treated with neoadjuvant S-1/cisplatin chemotherapy. Paraffin sections of pretreatment biopsy and surgical specimens from 41 gastric cancers ...

    journal_title：British journal of cancer

    pub_type： 杂志文章

    doi：10.1038/sj.bjc.6603546

    authors： Kamoshida S,Suzuki M,Shimomura R,Sakurai Y,Komori Y,Uyama I,Tsutsumi Y

    更新日期：2007-01-29 00:00:00

  • Detection of the anti-p53 antibody response in malignant and benign pancreatic disease.

    abstract：:Genomic alterations in the p53 tumour-suppressor gene and overexpression of p53 protein, resulting from gene mutations, are frequently found in pancreatic cancer. In this study we analysed the sera of 160 patients with malignant and benign pancreatic diseases for the presence of circulating antibodies to the p53 prote...

    journal_title：British journal of cancer

    pub_type： 杂志文章

    doi：10.1038/bjc.1994.443

    authors： Marxsen J,Schmiegel W,Röder C,Harder R,Juhl H,Henne-Bruns D,Kremer B,Kalthoff H

    更新日期：1994-11-01 00:00:00

  • Thymidine labelling studies in a transmissible venereal tumour of the dog.

    abstract：:The cell population kinetics of the transmissible venereal tumour of the dog was studied at two different stages of tumour growth using the labelled mitoses technique. At the first stage the tumours were growing with a doubling time of about 4 days; at the second stage their growth rate was limited, probably by an imm...

    journal_title：British journal of cancer

    pub_type： 杂志文章

    doi：10.1038/bjc.1972.54

    authors： Cohen D,Steel GG

    更新日期：1972-10-01 00:00:00

  • Analysis of acute vascular damage after photodynamic therapy using benzoporphyrin derivative (BPD).

    abstract：:Benzoporphyrin derivative monoacid ring A (BPD-MA, verteporfin) is currently under investigation as a photosensitizer for photodynamic therapy (PDT). Since BPD exhibits rapid pharmacokinetics in plasma and tissues, we assessed damage to tumour and muscle microvasculature when light treatment for PDT was given at short...

    journal_title：British journal of cancer

    pub_type： 杂志文章

    doi：10.1038/sj.bjc.6690271

    authors： Fingar VH,Kik PK,Haydon PS,Cerrito PB,Tseng M,Abang E,Wieman TJ

    更新日期：1999-04-01 00:00:00

  • Cardiotoxicity of commercial 5-fluorouracil vials stems from the alkaline hydrolysis of this drug.

    abstract：:The cardiotoxicity of 5-fluorouracil (FU) was attributed to impurities present in the injected vials. One of these impurities was identified as fluoroacetaldehyde which is metabolised by isolated perfused rabbit hearts into fluoroacetate (FAC), a highly cardiotoxic compound. FAC was also detected in the urine of patie...

    journal_title：British journal of cancer

    pub_type： 杂志文章

    doi：10.1038/bjc.1992.227

    authors： Lemaire L,Malet-Martino MC,de Forni M,Martino R,Lasserre B

    更新日期：1992-07-01 00:00:00

  • Antagonists of growth hormone-releasing hormone (GH-RH) inhibit IGF-II production and growth of HT-29 human colon cancers.

    abstract：:Insulin-like growth factors (IGFs) I and II are implicated in progression of various tumours including colorectal carcinomas. To interfere with the production of IGFs, we treated male nude mice bearing xenografts of HT-29 human colon cancer with various potent growth hormone-releasing hormone (GH-RH) antagonists. Twic...

    journal_title：British journal of cancer

    pub_type： 杂志文章

    doi：10.1054/bjoc.2000.1223

    authors： Szepeshazi K,Schally AV,Groot K,Armatis P,Halmos G,Herbert F,Szende B,Varga JL,Zarandi M

    更新日期：2000-05-01 00:00:00

  • Resistance to the mTOR-inhibitor RAD001 elevates integrin α2- and β1-triggered motility, migration and invasion of prostate cancer cells.

    abstract：BACKGROUND:Inhibitors of the mammalian target of rapamycin (mTOR) might become a novel tool to treat advanced prostate cancer. However, chronic drug exposure may trigger resistance, limiting the utility of mTOR inhibitors. METHODS:Metastatic potential of PC3 prostate cancer cells, susceptible (PC3(par)) or resistant (...

    journal_title：British journal of cancer

    pub_type： 杂志文章

    doi：10.1038/bjc.2012.313

    authors： Tsaur I,Makarević J,Juengel E,Gasser M,Waaga-Gasser AM,Kurosch M,Reiter M,Wedel S,Bartsch G,Haferkamp A,Wiesner C,Blaheta RA

    更新日期：2012-08-21 00:00:00

  • Do CA125 response criteria overestimate tumour response in second-line treatment of epithelial ovarian carcinoma?

    abstract：:Recent studies indicate that cancer antigen 125 (CA125) response criteria tend to overestimate a tumour reduction measured by standard WHO response criteria in recurrent epithelial ovarian carcinoma. The aim of the study was to validate the recently introduced GCIG (The Gynaecological Cancer Intergroup) CA125 response...

    journal_title：British journal of cancer

    pub_type： 临床试验,杂志文章

    doi：10.1038/sj.bjc.6601501

    authors： Gronlund B,Hansen HH,Høgdall C,Høgdall EV,Engelholm SA

    更新日期：2004-01-26 00:00:00

  • The incidence of B cell leukaemia and lymphopenia in B cell neoplasia in adults: a study using the Kiel classification of non-Hodgkin's lymphoma.

    abstract：:The incidence of B cell leukaemia in 186 consecutive untreated patients with histologically defined B cell neoplasms is described. The lymphomas were classified by the Kiel convention. B cell leukaemia in the context of this paper refers to the situation where a neoplastic clone of B cells in the blood greatly outnumb...

    journal_title：British journal of cancer

    pub_type： 杂志文章

    doi：10.1038/bjc.1983.174

    authors： Cader A,Richardson P,Walsh L,Ling NR,MacLennan IC,Jones EL,Leyland M

    更新日期：1983-08-01 00:00:00

  • The intramuscular administration of granulocyte colony-stimulating factor as an adjunct to chemotherapy in pretreated ovarian cancer patients: an Italian Trials in Medical Oncology (ITMO) Group pilot study.

    abstract：:No published data are available concerning the activity and tolerability of intramuscularly administered granulocyte colony-stimulating factor (G-CSF) in humans. To fill this gap, 19 patients with advanced ovarian cancer previously treated with at least one first-line chemotherapy cycle received the following myelosup...

    journal_title：British journal of cancer

    pub_type： 临床试验,杂志文章

    doi：10.1038/bjc.1994.186

    authors： Di Leo A,Bajetta E,Nolè F,Biganzoli L,Ferrari L,Oriana S,Riboldi G,Bohm S,Spatti G,Raspagliesi F

    更新日期：1994-05-01 00:00:00

  • Mutational analysis of the CDKN2 gene in metastases from patients with cutaneous malignant melanoma.

    abstract：:We analysed 26 metastases from 25 patients with sporadic cutaneous malignant melanoma for alterations in the CDKN2 gene by a combined polymerase chain reaction/single-strand conformation polymorphism (PCR/SSCP)/nucleotide sequencing approach. Eleven alterations (one in exon 1, five in exon 2 and five in the 3' non-cod...

    journal_title：British journal of cancer

    pub_type： 杂志文章

    doi：10.1038/bjc.1996.59

    authors： Platz A,Ringborg U,Lagerlöf B,Lundqvist E,Sevigny P,Inganäs M

    更新日期：1996-02-01 00:00:00

  • An RNAi-based screen reveals PLK1, CDK1 and NDC80 as potential therapeutic targets in malignant pleural mesothelioma.

    abstract：:This corrects the article DOI: 10.1038/bjc.2017.85. ...

    journal_title：British journal of cancer

    pub_type： 杂志文章,已发布勘误

    doi：10.1038/bjc.2018.3

    authors： Linton A,Cheng YY,Griggs K,Schedlich L,Kirschner MB,Gattani S,Srikaran S,Chuan-Hao Kao S,McCaughan BC,Klebe S,van Zandwijk N,Reid G

    更新日期：2018-03-20 00:00:00

  • Systemic immune-inflammation index in germ-cell tumours.

    abstract：BACKGROUND:We evaluated systemic immune-inflammation index (SII) and its association with patient outcome in germ-cell tumours (GCTs). METHODS:Two independent cohorts of patients were analysed; the discovery set (n=171) from a single institution and the validation set (n=181) previously included in a study evaluating ...

    journal_title：British journal of cancer

    pub_type： 杂志文章

    doi：10.1038/bjc.2017.460

    authors： Chovanec M,Cierna Z,Miskovska V,Machalekova K,Kalavska K,Rejlekova K,Svetlovska D,Macak D,Spanik S,Kajo K,Babal P,De Giorgi U,Mego M,Mardiak J

    更新日期：2018-03-20 00:00:00

  • Stimulation of erythropoiesis by the non-steroidal anti-androgen nilutamide in men with prostate cancer: evidence for an agonistic effect?

    abstract：:The effects of steroid hormones are pleiotropic. Similarly, non-steroidal oestrogen receptor antagonists such as tamoxifen exert partial agonistic effects with a species- and tissue-specific pattern. Conversely, little is known of the biological effects of non-steroidal anti-androgens, whose role has been investigated...

    journal_title：British journal of cancer

    pub_type： 临床试验,杂志文章,随机对照试验

    doi：10.1038/bjc.1994.115

    authors： Decensi A,Torrisi R,Fontana V

    更新日期：1994-03-01 00:00:00

  • Prognostic implication of the CpG island methylator phenotype in colorectal cancers depends on tumour location.

    abstract：BACKGROUND:Colorectal cancer (CRC) is usually categorised as proximal or distal CRC. Recently, many researchers have tried to determine the molecular heterogeneity of CRCs along bowel subsites. However, the differential effects of the CpG island methylator phenotype (CIMP) and microsatellite instability (MSI) on the cl...

    journal_title：British journal of cancer

    pub_type： 杂志文章

    doi：10.1038/bjc.2013.430

    authors： Bae JM,Kim JH,Cho NY,Kim TY,Kang GH

    更新日期：2013-08-20 00:00:00

  • Evaluation of cumulative prognostic scores based on the systemic inflammatory response in patients with inoperable non-small-cell lung cancer.

    abstract：:A score based on the combination of the systemic inflammatory response and albumin hazards ratio (HR) 1.70, 95% CI 1.23 - 2.35, P=0.001) was comparable in prognostic value to that based on stage and performance status (HR 1.48, 95% CI 1.12 - 1.95, P=0.006) in patients with inoperable non-small-cell lung cancer. The fo...

    journal_title：British journal of cancer

    pub_type： 杂志文章

    doi：10.1038/sj.bjc.6601242

    authors： Forrest LM,McMillan DC,McArdle CS,Angerson WJ,Dunlop DJ

    更新日期：2003-09-15 00:00:00