Abstract:
:Helicobacter pylori is present in infected patients not only on the gastric epithelial cell surface but also in gastric mucus. We developed a competitive polymerase chain reaction (cPCR) method for quantitative measurement of H. pylori in gastric mucus. The aim of this study was to determine the number of H. pylori in gastric mucus before and after anti-H. pylori treatment. Patients with duodenal ulcer were treated with lansoprazole alone (n = 11) or lansoprazole and amoxycillin (n = 12). The amount of H. pylori in gastric mucus was measured over time by a cPCR assay. Helicobacter pylori infection was also tested for using histology, culture, and the rapid urease test (RUT). Although most patients treated with lansoprazole alone had become H. pylori-negative by the end of treatment when tested by histology, RUT, and culture, a large number of H. pylori organisms were found in the gastric mucus at that time by cPCR. These patients returned to being H. pylori positive 1 to 12 months later on the basis of histology, RUT, and culture. However, cPCR results indicated eradication of H. pylori by the end of treatment in eight of the 12 patients treated with lansoprazole and amoxicillin, and these patients remained H. pylori negative on histology, RUT, culture, and cPCR 1 to 12 months later. Testing for H. pylori in gastric mucus is thus useful for precise determination of the success or failure of H. pylori eradication therapy.
journal_name
J Gastroenterol Hepatoljournal_title
Journal of gastroenterology and hepatologyauthors
Furuta T,Takashima M,Futami H,Arai H,Hanai H,Kaneko Edoi
10.1111/j.1440-1746.1998.tb00678.xsubject
Has Abstractpub_date
1998-05-01 00:00:00pages
511-5issue
5eissn
0815-9319issn
1440-1746journal_volume
13pub_type
临床试验,杂志文章,随机对照试验abstract::The incidence of inflammatory bowel diseases (IBD) in East has risen over the past decade to become a global disease. The increasing number of studies on the incidence and course of IBD in East has enabled us to explore East versus West differences in the epidemiology of IBD which could enhance our understanding of th...
journal_title:Journal of gastroenterology and hepatology
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journal_title:Journal of gastroenterology and hepatology
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journal_title:Journal of gastroenterology and hepatology
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journal_title:Journal of gastroenterology and hepatology
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journal_title:Journal of gastroenterology and hepatology
pub_type: 杂志文章
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pub_type: 杂志文章,随机对照试验
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journal_title:Journal of gastroenterology and hepatology
pub_type: 共识发展会议,杂志文章,评审
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