Abstract:
:Although TGF-β and IL-6 would turn CD8(+) T cells to differentiate into non-cytotoxic state, these treated cells were converted to cytolytic phenotypes after re-exposure to their antigenic epitope in vitro. Here, using spleen cells from TCR transgenic mice expressing TCRαβ genes of clone RT1 recognizing an epitope peptide (P18-I10: RGPGRAFVTI) of HIV-1 gp160, we generated CD8(+) cytotoxic T lymphocytes (CTLs) activated by re-exposure to P18-I10 after primarily cultured with TGF-β and IL-6 in vitro to examine their effector function. The CTLs, having strong cytotoxic activity in vitro, were not only resistant to Fas-FasL mediated apoptosis, but also insensitive to the suppression of their cytotoxicity by re-exposure to TGF-β in vitro. Moreover, adoptive transfer experiments indicated that the CTLs are capable of eliminating recombinant vaccinia virus expressing HIV-1 gp160 in vivo. Taken together, our data suggest that TGF-β and IL-6 may play pivotal roles in inducing apoptosis-resistant and TGF-β-insensitive CTLs in vitro.
journal_name
Cell Immunoljournal_title
Cellular immunologyauthors
Takaku S,Nakagawa Y,Owaki A,Shimizu M,Takahashi M,Takahashi Hdoi
10.1016/j.cellimm.2012.12.008subject
Has Abstractpub_date
2012-12-01 00:00:00pages
138-47issue
2eissn
0008-8749issn
1090-2163pii
S0008-8749(13)00005-1journal_volume
280pub_type
杂志文章abstract::Experimental autoimmune encephalomyelitis (EAE) is an animal model for multiple sclerosis (MS), and is induced by immunization with disease-causative self-antigens such as myelin oligodendrocyte glycoprotein (MOG). We have previously reported that transplantation of MOG expressing thymic epithelial progenitors (TEPs) ...
journal_title:Cellular immunology
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journal_title:Cellular immunology
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journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(90)90286-z
更新日期:1990-10-15 00:00:00
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journal_title:Cellular immunology
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journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2009.05.017
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journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(86)90340-0
更新日期:1986-10-01 00:00:00
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pub_type: 杂志文章
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journal_title:Cellular immunology
pub_type: 杂志文章
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journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1993.1293
更新日期:1993-12-01 00:00:00
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journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(86)90268-6
更新日期:1986-03-01 00:00:00
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journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(86)90341-2
更新日期:1986-10-01 00:00:00
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journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(84)90059-5
更新日期:1984-10-01 00:00:00
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journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1997.1165
更新日期:1997-08-01 00:00:00
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journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(84)90105-9
更新日期:1984-04-01 00:00:00
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journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2010.04.001
更新日期:2010-01-01 00:00:00
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journal_title:Cellular immunology
pub_type: 杂志文章
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journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1994.1230
更新日期:1994-09-01 00:00:00
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journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2011.01.007
更新日期:2011-01-01 00:00:00
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journal_title:Cellular immunology
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更新日期:1989-05-01 00:00:00
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journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(89)90291-8
更新日期:1989-10-15 00:00:00
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journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.1997.1180
更新日期:1997-10-10 00:00:00
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journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(86)90100-0
更新日期:1986-12-01 00:00:00
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journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/j.cellimm.2020.104100
更新日期:2020-06-01 00:00:00
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journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1006/cimm.2000.1729
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journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(88)90180-3
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journal_title:Cellular immunology
pub_type: 杂志文章
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更新日期:1987-05-01 00:00:00
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journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(85)90248-5
更新日期:1985-04-01 00:00:00
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journal_title:Cellular immunology
pub_type: 杂志文章
doi:10.1016/0008-8749(90)90078-6
更新日期:1990-01-01 00:00:00