Abstract:
BACKGROUND:Gene therapy is a promising therapeutic method but is severely hampered due to its lack of an ideal delivery system. Therefore, in this study, a nonviral and magnetic resonance imaging (MRI) visible vector, polyethylene glycol-grafted polyethylenimine and superparamagnetic iron oxide nanoparticles (PEG-g-PEI-SPION) was used as a nanocarrier for small interfering RNA (siRNA) delivery in gastric cancer. METHODS:Biophysical characterization of PEG-g-PEI-SPION was systematically analyzed, including size, zeta potential, siRNA condensation capacity, cell viability, transfection efficiency, cellular uptake, and MRI-visible function in vivo. Besides, CD44 variant isoform 6 (CD44v6), a protein marker for metastatic behavior in gastric cancer, and was chose as the target gene to further analyze the siRNA delivery function of PEG-g-PEI-SPION. RESULTS:Under comprehensive analysis, the appropriate N/P ratio of PEG-g-PEI-SPION/siRNA was 10, and siRNA targeting at human CD44v6 (siCD44v6) transferred by PEG-g-PEI-SPION was effective at downregulating the CD44v6 expression of gastric carcinoma cell line SGC-7901 in vitro. Moreover, knockdown of CD44v6 impaired migrating and invasive abilities of SGC-7901 cells. Furthermore, PEG-g-PEI-SPION was a highly efficient contrast agent for MRI scan in vivo. CONCLUSION:PEG-g-PEI-SPION was a promising nonviral vector with molecular image tracing capacity for cancer gene therapy. And CD44v6 was a potential target gene for the prevention and detection of metastatic behavior in gastric cancer.
journal_name
J Gastroenteroljournal_title
Journal of gastroenterologyauthors
Chen Y,Lian G,Liao C,Wang W,Zeng L,Qian C,Huang K,Shuai Xdoi
10.1007/s00535-012-0713-xsubject
Has Abstractpub_date
2013-07-01 00:00:00pages
809-21issue
7eissn
0944-1174issn
1435-5922journal_volume
48pub_type
杂志文章abstract:BACKGROUND:Peristaltic reflex does not fully explain the polarity of peristalsis. In the distal colon, we propose that the balance of circular muscle contraction proximal and distal to the pellet is a local reflex that predicts polarity of peristalsis. METHODS:Guinea pig distal colon segments were harvested, and fecal...
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doi:
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更新日期:1999-02-01 00:00:00
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journal_title:Journal of gastroenterology
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更新日期:1996-02-01 00:00:00
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更新日期:1994-04-01 00:00:00
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doi:
更新日期:2003-03-01 00:00:00
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pub_type: 临床试验,杂志文章,多中心研究,随机对照试验
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