Abstract:
BACKGROUND:Radiation-induced wound healing complications represent an important clinical problem. Microvascular compromise is an important component of its pathogenesis and the microvascular endothelial cell is the key representative affected at the cellular level. MATERIAL AND METHODS:Human dermal microvascular endothelial cells (HDMEC) were cultured and irradiated with doses of 2 to 12 Gy. Cell density was determined 48 h after radiation using a semi-automated cell counting system. Levels of interleukin-6 (IL-6), basic fibroblast growth factor (FGF), intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) in the supernatants of HDMEC were determined by polymerase chain reaction (PCR) and enzyme-linked immunosorbent assay (ELISA). Non irradiated HDMEC were used as controls. RESULTS:Cell density was significantly impaired in irradiated cells compared to non irradiated controls. Radiation resulted in significant elevation of levels of IL-6, FGF, ICAM-1 and VCAM-1 in the supernatants of HDMEC in a dose dependent manner. CONCLUSION:The inflammatory response observed clinically after radiation seems to correlate with elevated expression of cytokines and adhesion molecules by microvascula endothelial cells. The model of HDMEC documents the impairment of microcirculation. These in vitro changes may enhance our understanding of the pathomechanisms leading to radiation-induced vasculitis and associated wound healing problems.
journal_name
Clin Hemorheol Microcircjournal_title
Clinical hemorheology and microcirculationauthors
Haubner F,Ohmann E,Pohl F,Prantl L,Strutz J,Gassner HGdoi
10.3233/CH-2012-1626subject
Has Abstractpub_date
2013-01-01 00:00:00pages
371-9issue
4eissn
1386-0291issn
1875-8622pii
D43882J2366LW381journal_volume
54pub_type
杂志文章abstract::Asian tradition medicine (ATM), such as herbal medicine and acupuncture, has been widely used in the treatment of diseases, but the effects still remain unclear at the level of the microcirculation. Up to now, a variety of approaches have been made to comprehensively evaluate the significance of ATM. Microcirculatory ...
journal_title:Clinical hemorheology and microcirculation
pub_type: 杂志文章
doi:
更新日期:2000-01-01 00:00:00
abstract::After rabbit's erythrocytes were treated with different concentrations of intercellular Ca2+ and ionophore A23187 respectively, the orientation index (DI)or, and small deformation index (DI)d of rabbit erythrocytes were measured by new model ektacytometry. The results demonstrated that (DI)or and (DI)d decreased while...
journal_title:Clinical hemorheology and microcirculation
pub_type: 杂志文章
doi:
更新日期:2001-01-01 00:00:00
abstract::Determination of shear stresses at given shear rates allow approximation of flow curves by mathematical models and to calculate viscosities of non-Newtonian fluids. In term neonates, the mean arterial blood pressure (MAP) is markedly below that of adults, therefore rheological properties of blood play an important rol...
journal_title:Clinical hemorheology and microcirculation
pub_type: 杂志文章
doi:10.3233/CH-152008
更新日期:2015-10-06 00:00:00
abstract::It is well established that exercise performance in athletes is related to improved blood fluidity. However, training effects on functional state of endothelium and relations of endothelial cell functions with hemorheology are poorly known. Circulating levels of von Willebrand factor may serve as a good marker of endo...
journal_title:Clinical hemorheology and microcirculation
pub_type: 杂志文章
doi:
更新日期:2003-01-01 00:00:00
abstract::Thrombotic events result from different pathologies and are the underlying causes of severe diseases like stroke or myocardial infarction. Recent basic research now revealed a link between food uptake, food conversion and gut metabolism. Gut microbial production of trimethylamine N-oxide (TMAO) from dietary nutrients ...
journal_title:Clinical hemorheology and microcirculation
pub_type: 杂志文章
doi:10.3233/CH-209206
更新日期:2020-01-01 00:00:00
abstract:BACKGROUND:Microvascular dysfunction is a main contributor to morbidity and mortality worldwide. Sophisticated technical tools (e.g. miniaturized hardware, automated software), along with skilled personnel are the prerequisite for quantitative observations of the microvasculature. OBJECTIVE:This review aimed to get an...
journal_title:Clinical hemorheology and microcirculation
pub_type: 杂志文章,评审
doi:10.3233/CH-190611
更新日期:2019-01-01 00:00:00
abstract::In order to further characterize the alterations of erythrocyte aggregation described in ovarian cancer, we measured it with laser backscattering in eleven women suffering from ovarian cancer (mean age: 44.7 +/- 3.6, extreme values: 28-61 yr) compared with thirteen matched control women. Blood rheology exhibited a wid...
journal_title:Clinical hemorheology and microcirculation
pub_type: 杂志文章
doi:
更新日期:2000-01-01 00:00:00
abstract::Vertigo of various and often unknown aetiologies has been associated with and attributed to impaired microvascular perfusion in the inner ear or the vertebrobasilar system. Vertigoheel is a low-dose combination preparation of proven value in the symptomatic treatment of vertigo. In the present study we tested the hypo...
journal_title:Clinical hemorheology and microcirculation
pub_type: 杂志文章
doi:10.3233/CH-2010-1330
更新日期:2010-01-01 00:00:00
abstract::Beta-thalassaemia is a hereditary hemolytic disease, in which each clinic phenotype encompasses a heterogenic group of genetic alterations resulting in beta-globin chain synthesis decrease or absence in red blood cells. Studies on beta-thalassaemia carriers suggest the existence of decreased red cell deformability. Th...
journal_title:Clinical hemorheology and microcirculation
pub_type: 杂志文章
doi:
更新日期:2006-01-01 00:00:00
abstract::Red blood cells (RBC) of neonates have a shorter survival time and they are more susceptible to mechanical alterations than RBC of adults. Irreversible alteration of the membrane of RBC of preterm neonates, term neonates and adults due to tether formation was studied by means of a micropipette technique. Shear stress ...
journal_title:Clinical hemorheology and microcirculation
pub_type: 杂志文章
doi:10.3233/CH-2011-1390
更新日期:2011-01-01 00:00:00
abstract:OBJECTIVE:This study aims to investigate the effect of nimodipine combined with betahistine on the levels of CRP and other inflammatory cytokines, as well as vascular endothelial function in patients with hypertensive cerebral vasospasm. METHODS:A total of 80 patients with hypertensive cerebral vasospasm from March 20...
journal_title:Clinical hemorheology and microcirculation
pub_type: 杂志文章
doi:10.3233/CH-190589
更新日期:2020-01-01 00:00:00
abstract::Some studies indicate that obesity is associated with rheological disturbances. Because there are usually sex differences in the type of obesity we decided to evaluate rheological differences between male and female obese patients. We studied 18 morbidly obese men, mean age 43.66 +/- 11.32 years, mean body mass index ...
journal_title:Clinical hemorheology and microcirculation
pub_type: 杂志文章
doi:10.3233/CH-2010-1275
更新日期:2010-01-01 00:00:00
abstract::The diabetic vasculopathy is one of the major complications responsible for the high incidence of arteriopathy, coronary ischemia and renal failure. Several hypothesis have been formulated to explain the vascular abnormalities. We recently showed that advanced glycation end products (AGE) have a pivotal role in the ge...
journal_title:Clinical hemorheology and microcirculation
pub_type: 杂志文章,评审
doi:
更新日期:2001-01-01 00:00:00
abstract:BACKGROUND:The ability to evaluate tumor development within experimental oncology is of upmost importance. However, determining tumor volumes in 3D in vivo tumor models is challenging. The chick chorioallantoic membrane (CAM) model represents an optimized xenograft model that surpasses many disadvantages that are inher...
journal_title:Clinical hemorheology and microcirculation
pub_type: 杂志文章
doi:10.3233/CH-209216
更新日期:2020-01-01 00:00:00
abstract:BACKGROUND:Purpura fulminans (PF) is a distinct form of rare meningococcal septicaemia mostly in childhood which is characterized by high lethality, extensive necroses and mutilations of extremities. Other ethiologies are idiopathic forms or purpura neonatorum, which is marked by deficiency of Protein-C. PF is caused b...
journal_title:Clinical hemorheology and microcirculation
pub_type: 杂志文章
doi:10.3233/CH-190588
更新日期:2020-01-01 00:00:00
abstract::The study aimed to investigate local thermally induced microvascular reactivity in patients with type 1 (T1DM) or type 2 diabetes mellitus (T2DM) and polyneuropathy and to compare it with healthy controls. A hundred and fourteen subjects were investigated divided into 3 groups: 1st group -20 patients with T1DM; 2nd gr...
journal_title:Clinical hemorheology and microcirculation
pub_type: 杂志文章
doi:10.3233/CH-15107
更新日期:2017-01-01 00:00:00
abstract:BACKGROUND AND AIM:Hematocrit is an important hemorheological parameter. Both hematocrit and nonalcoholic fatty liver disease (NAFLD) are strongly correlated with cardiovascular disease. However, there is no study to explore the direct relationship of hematocrit with NAFLD. METHODS:Hematocrit levels were analyzed from...
journal_title:Clinical hemorheology and microcirculation
pub_type: 杂志文章
doi:10.3233/CH-2011-1509
更新日期:2012-01-01 00:00:00
abstract::A simple and reproducible 'syringe viscometer' method was used to determine plasma viscosity, an important haemorheological parameter, in 27 (14 M, 13 F) nephrotic Nigerian patients. The plasma fibrinogen (PFC) and immunoglobulin levels (IgG, IgM, IgA) were also determined in these patients. Similar determinations wer...
journal_title:Clinical hemorheology and microcirculation
pub_type: 杂志文章
doi:
更新日期:1998-10-01 00:00:00
abstract:OBJECTIVE:To assess sex-related differences in forearm skin microvascular reactivity to vascular occlusion and cardiovascular health in young healthy women and men. METHODS:203 young healthy individuals (67 men/136 women) participated in this study. Post-occlusive reactive hyperemia (PORH) in skin microcirculation fol...
journal_title:Clinical hemorheology and microcirculation
pub_type: 杂志文章
doi:10.3233/CH-180483
更新日期:2019-01-01 00:00:00
abstract::The erythrocyte adenosine triphosphate (ATP) is utilised for protein phosphorylation and exported through the pannexin 1 hemichannel (Px1) in the microcirculation. The physiological stimuli for ATP release are dependent of blood shear rate level and of the tissue oxygen content. The deoxygenated and oxygenated states ...
journal_title:Clinical hemorheology and microcirculation
pub_type: 杂志文章
doi:10.3233/CH-141833
更新日期:2015-01-01 00:00:00
abstract::General anesthesia, either with inhalation or through nonvolatile anesthetics, is known to affect the overall cardiovascular function as well as the microcirculatory hemodynamics. In this study, the effects of desfluran anesthesia on the red blood cell deformability of young and old rats are investigated. 33 male rats...
journal_title:Clinical hemorheology and microcirculation
pub_type: 杂志文章
doi:
更新日期:2006-01-01 00:00:00
abstract:BACKGROUND:Hemorheologic factors such as red blood cell (RBC) aggregation and deformability differ according to species. In many comparative hemorheologic studies, only venous blood samples have been used for measurements. There is little published information comparing arterial and venous blood differences between spe...
journal_title:Clinical hemorheology and microcirculation
pub_type: 杂志文章
doi:10.3233/CH-2010-1248
更新日期:2010-01-01 00:00:00
abstract::The hemorheological performance of mammals and human will change with the time after the blood is withdraw from the subject. The whole blood apparent viscosity, erythrocyte deformability and aggregation of some mammals and human were measured. Our results showed that the hemorheological characteristics of mice, rats, ...
journal_title:Clinical hemorheology and microcirculation
pub_type: 杂志文章
doi:
更新日期:2004-01-01 00:00:00
abstract::The contribution of hemorheological alterations in the prothrombotic condition in patients with metabolic syndrome (MS) remains a question of debate. We aimed to determine the association between MS and hemorheological parameters by means of a case-control study in 61 MS patients and 89 controls without MS. We determi...
journal_title:Clinical hemorheology and microcirculation
pub_type: 杂志文章
doi:10.3233/CH-2011-1499
更新日期:2011-01-01 00:00:00
abstract::Although microcirculatory disturbances play pivotal role in the pathomechanism of acute pancreatitis (AP), very few papers can be found which had been tested any of hemorheological parameters. The aim of our study was to analyze the hemorheological changes in cerulein-induced experimental acute pancreatitis in rat in ...
journal_title:Clinical hemorheology and microcirculation
pub_type: 杂志文章
doi:10.3233/CH-2012-1531
更新日期:2012-01-01 00:00:00
abstract::Radiographic contrast media can lead to drastic changes of the morphology of erythrocytes. The change of the erythrocyte morphology is associated with a decreased deformability possibly resulting from distinctions in the loss of constituents of the membrane cytoskeleton. However, it is unclear whether there is an intr...
journal_title:Clinical hemorheology and microcirculation
pub_type: 杂志文章
doi:10.3233/CH-141879
更新日期:2014-01-01 00:00:00
abstract:PURPOSE:Real-time shear-wave elastography (SWE) is a newly developed technique for the sonographic quantification of tissue elasticity, which already is used in the assessment of breast and thyroid lesions. Due to limited overlying tissue, the testes are ideally suited for assessment using shear wave elastography. To o...
journal_title:Clinical hemorheology and microcirculation
pub_type: 杂志文章
doi:10.3233/CH-162046
更新日期:2016-01-01 00:00:00
abstract::This experiment was designed to study the therapeutic mechanisms of Angelica on the focal cerebral ischemia injury of the rat. The ischemic area was determined by TTC stain. And terminal deoxynucleotidyl transferase (TDT) mediated DUTP-biotin nick end labeling (TUNEL) method was applied to detect neuronal apoptosis. T...
journal_title:Clinical hemorheology and microcirculation
pub_type: 杂志文章
doi:
更新日期:2005-01-01 00:00:00
abstract:BACKGROUND:Doxorubicin is a cytostatic drug from the group of anthracycline antibiotics that is widely used as a chemotherapeutic agent. Side effects of the active substance include cardiotoxicity and nephrotoxicity. Doxorubicin-treated renal epithelial cells and (sarcoma) tumors are examined by correlative light and e...
journal_title:Clinical hemorheology and microcirculation
pub_type: 杂志文章
doi:10.3233/CH-199212
更新日期:2019-01-01 00:00:00
abstract:OBJECTIVE:IL-17 is considered to be a cancer-promoting gene in hepatocellular carcinoma (HCC). Here, we explored the effect of IL-17 in predicting the therapeutic effect of transcatheter arterial chemoembolization (TACE) combined with apartinib in patients with HCC in this study. METHODS:Established of IL-17 knockdown...
journal_title:Clinical hemorheology and microcirculation
pub_type: 杂志文章
doi:10.3233/CH-200857
更新日期:2020-07-09 00:00:00