Abstract：
:Metastatic cancer cells produce invasive membrane protrusions called invadopodia and pseudopodia, which play a central role in driving cancer cell dissemination in the body. Malignant cells use these structures to attach to and degrade extracellular matrix proteins, generate force for cell locomotion, and to penetrate the vasculature. Recent work using unique subcellular fractionation methodologies combined with spatial genomic, proteomic, and phosphoproteomic profiling has provided insight into the invadopodiome and pseudopodiome signaling networks that control the protrusion of invasive membranes. Here I highlight how these powerful spatial 'omics' approaches reveal important signatures of metastatic cancer cells and possible new therapeutic targets aimed at treating metastatic disease.
journal_name
Curr Opin Cell Bioljournal_title
Current opinion in cell biologyauthors
Klemke RLdoi
10.1016/j.ceb.2012.08.005subject
Has Abstractpub_date
2012-10-01 00:00:00pages
662-9issue
5eissn
0955-0674issn
1879-0410pii
S0955-0674(12)00130-5journal_volume
24pub_type
杂志文章,评审abstract：:The histones which pack new DNA during the S phase of animal cells are made from mRNAs that are cleaved at their 3' end but not polyadenylated. Some of the factors used in this reaction are unique to it while others are shared with the polyadenylation process that generates all other mRNAs. Recent work has begun to sh...
journal_title：Current opinion in cell biology
pub_type： 杂志文章,评审
doi：10.1016/j.ceb.2016.01.015
更新日期：2016-06-01 00:00:00
abstract：:Analysis of complex and redundant pathways that control proliferation, differentiation, apoptosis and DNA damage response by global genome wide analysis is an intensive area of investigation aimed at identifying unique molecular signatures of prognostic significance in cancer. An alternative approach is to focus on th...
journal_title：Current opinion in cell biology
pub_type： 杂志文章,评审
doi：10.1016/j.ceb.2007.10.005
更新日期：2007-12-01 00:00:00
abstract：:When PI3Ks are deregulated by aberrant surface receptors or modulators, accumulation of PtdIns(3,4,5)P3 leads to increased cell growth, proliferation and contact-independent survival. The PI3K/PKB/TOR axis controls protein synthesis and growth, while PtdIns(3,4,5)P3-mediated activation of Rho GTPases directs cell moti...
journal_title：Current opinion in cell biology
pub_type： 杂志文章,评审
doi：10.1016/j.ceb.2005.02.011
更新日期：2005-04-01 00:00:00
abstract：:Mammalian Unc-51-like kinases 1 and 2 (ULK1 and ULK2) belong to the ULK/Atg1 family of serine/threonine kinases, which are conserved from yeast to mammals. Although ULK/Atg1 is best known for regulating flux through the autophagy pathway, it has evolutionarily conserved noncanonical functions in protein trafficking th...
journal_title：Current opinion in cell biology
pub_type： 杂志文章,评审
doi：10.1016/j.ceb.2017.02.011
更新日期：2017-04-01 00:00:00
abstract：:Like other spectrin repeat proteins, nesprins co-ordinate and maintain cellular architecture by linking membranous organelles to the cytoskeleton. However nuclear envelope (NE) nesprins, uniquely hardwire the nuclear lamina to the cytoskeleton and molecular motors. Emerging evidence suggests that nesprins also form a ...
journal_title：Current opinion in cell biology
pub_type： 杂志文章,评审
doi：10.1016/j.ceb.2010.11.006
更新日期：2011-02-01 00:00:00
abstract：:The three germ layers - mesoderm, endoderm and ectoderm - constituting the cellular blueprint for the tissues and organs that will form during embryonic development, are specified at gastrulation. Cells of mesodermal origin are the most abundant in the human body, representing a great variety of cell types, including ...
journal_title：Current opinion in cell biology
pub_type： 杂志文章,评审
doi：10.1016/j.ceb.2019.07.012
更新日期：2019-12-01 00:00:00
abstract：:New techniques have enhanced our picture of E2F regulation. These studies have shed light on the roles played by individual E2F and retinoblastoma family members and implicate these proteins in processes extending well beyond the G1/S transition. One thorny issue remains: do our current molecular models of E2F and ret...
journal_title：Current opinion in cell biology
pub_type： 杂志文章,评审
doi：10.1016/s0955-0674(02)00388-5
更新日期：2002-12-01 00:00:00
abstract：:It is widely recognized that after endocytosis, internalized cargo is delivered to endosomes that act as sorting stations. The limiting membrane of endosomes contain specialized subregions, or microdomains, that represent distinct functions of the endosome, including regions competing for cargo capture leading to degr...
journal_title：Current opinion in cell biology
pub_type： 杂志文章,评审
doi：10.1016/j.ceb.2020.02.018
更新日期：2020-08-01 00:00:00
abstract：:How are cell morphogenesis and cell cycle coordinated? The fission yeast is a rod-shaped unicellular organism widely used to study how a cell self-organizes in space and time. Here, we discuss recent advances in understanding how the cell acquires and maintains its regular rod shape and uses it to control cell divisio...
journal_title：Current opinion in cell biology
pub_type： 杂志文章,评审
doi：10.1016/j.ceb.2012.10.001
更新日期：2012-12-01 00:00:00
abstract：:Far from being simple 'bags' of enzymes, bacteria are richly endowed with ultrastructures that challenge and expand standard definitions of the cytoskeleton. Here we review rods, rings, twisted pairs, tubes, sheets, spirals, moving patches, meshes and composites, and suggest defining the term 'bacterial cytoskeleton' ...
journal_title：Current opinion in cell biology
pub_type： 杂志文章,评审
doi：10.1016/j.ceb.2012.10.019
更新日期：2013-02-01 00:00:00
abstract：:There are, in theory, several ways in which proteins may pass through the Golgi apparatus. Among these, the cisternal progression-maturation mode has gained broad consensus. However, there remain questions regarding the molecular mechanisms by which resident proteins are sorted from cargo and move backward to the prox...
journal_title：Current opinion in cell biology
pub_type： 杂志文章,评审
doi：10.1016/j.ceb.2010.05.003
更新日期：2010-08-01 00:00:00
abstract：:Recently, findings regarding a group of cancer predisposition and chromosome instability syndromes, Nijmegen breakage syndrome (NBS), the ataxia-telangiectasia-like disorder (A-TLD) and ataxia telangiectasia have shed light on the unexpected role of recombinational DNA repair proteins in DNA-damage-dependent cell-cycl...
journal_title：Current opinion in cell biology
pub_type： 杂志文章,评审
doi：10.1016/s0955-0674(00)00091-0
更新日期：2000-06-01 00:00:00
abstract：:Misfolded and mistargeted proteins in the early secretory pathway present significant risks to the cell. A diverse and integrated network of quality control pathways protects the cell from these threats. We focus on the discovery of new mechanisms that contribute to this protective network. Biochemical and structural ...
journal_title：Current opinion in cell biology
pub_type： 杂志文章,评审
doi：10.1016/j.ceb.2020.04.002
更新日期：2020-08-01 00:00:00
abstract：:The proposition is forwarded that the cAMP-dependent protein kinase is one of quite a small class of enzymes wherein differential modes of binding of its multiple substrates make an important contribution to the end physiological response. It is postulated that a variety of different substrate affinities may have evol...
journal_title：Current opinion in cell biology
pub_type： 杂志文章,评审
doi：10.1016/0955-0674(92)90039-f
更新日期：1992-04-01 00:00:00
abstract：:Cohesin, one of structural maintenance of chromosomes (SMC) complexes, forms a ring-shaped protein complex, and mediates sister chromatid cohesion for accurate chromosome segregation and precise genome inheritance. The cohesin ring entraps one or two DNA molecules to achieve cohesion, which is further regulated by coh...
journal_title：Current opinion in cell biology
pub_type： 杂志文章,评审
doi：10.1016/j.ceb.2018.11.006
更新日期：2019-06-01 00:00:00
abstract：:Actin polymerization generates the force that deforms the cell membrane, pulls the cell forward and propels endosomes and bacteria within the cell. The mechanism of force generation has been probed using experimental biomimetic systems where force generation and movement occur by the same actin-polymerization processe...
journal_title：Current opinion in cell biology
pub_type： 杂志文章,评审
doi：10.1016/j.ceb.2004.12.001
更新日期：2005-02-01 00:00:00
abstract：:New structural analyses of the spectrin family of actin cross-linking proteins are providing molecular explanations for both the interchain binding between the alpha and beta chains of spectrin and the intermolecular associations between spectrin and other proteins. Additionally, the analyses bring into focus a confor...
journal_title：Current opinion in cell biology
pub_type： 杂志文章,评审
doi：10.1016/s0955-0674(96)80048-2
更新日期：1996-02-01 00:00:00
abstract：:Studies of various membrane trafficking steps over the past year indicate that membranes are tethered together prior to the interaction of v-SNAREs and t-SNAREs across the membrane junction. The tethering proteins identified to date are quite large, being either fibrous proteins or multimeric protein complexes. The te...
journal_title：Current opinion in cell biology
pub_type： 杂志文章,评审
doi：10.1016/s0955-0674(99)80065-9
更新日期：1999-08-01 00:00:00
abstract：:At the time of this writing, searching Google Scholar for 'light-sheet microscopy' returns almost 8500 results; over three-quarters of which were published in the last 5 years alone. Searching for other advanced imaging methods in the last 5 years yields similar results: 'super-resolution microscopy' (>16 000), 'singl...
journal_title：Current opinion in cell biology
pub_type： 杂志文章,评审
doi：10.1016/j.ceb.2020.04.008
更新日期：2020-10-01 00:00:00
abstract：:A number of proteins that are necessary for membrane transport have been identified using cell-free assays and yeast genetics. Although our knowledge of transport mechanisms remains limited, common themes are clearly emerging. In particular, specific GTP-binding proteins appear to be involved, not only at all steps of...
journal_title：Current opinion in cell biology
pub_type： 杂志文章,评审
doi：10.1016/0955-0674(92)90077-p
更新日期：1992-08-01 00:00:00
abstract：:Most cancers arise in epithelia, the tissue type that lines all body cavities. The organization of epithelia enables them to act as a barrier and perform vectorial transport of molecules between body compartments. Crucial for their organization and function is a highly specialized network of cell adhesion and polarity...
journal_title：Current opinion in cell biology
pub_type： 杂志文章,评审
doi：10.1016/j.ceb.2013.11.003
更新日期：2014-02-01 00:00:00
abstract：:Evolution of the mammalian sex chromosomes heavily impacts on the expression of X-encoded genes, both in marsupials and placental mammals. The loss of genes from the Y chromosome forced a two-fold upregulation of dose sensitive X-linked homologues. As a corollary, female cells would experience a lethal dose of X-linke...
journal_title：Current opinion in cell biology
pub_type： 杂志文章,评审
doi：10.1016/j.ceb.2012.02.004
更新日期：2012-06-01 00:00:00
abstract：:The recent introduction of positional proteomics made it possible to screen for protease processing events on a proteome-wide scale. As a highly regulated and protease-dependent process, cell death has been particularly well-studied with these emerging technologies. This review provides an overview of the results obta...
journal_title：Current opinion in cell biology
pub_type： 杂志文章,评审
doi：10.1016/j.ceb.2010.08.021
更新日期：2010-12-01 00:00:00
abstract：:Integrins are heterodimeric transmembrane receptors that connect the extracellular matrix environment to the actin cytoskeleton via adaptor molecules through assembly of a range of adhesion structures. Recent advances in biochemical, imaging and biophysical methods have enabled a deeper understanding of integrin signa...
journal_title：Current opinion in cell biology
pub_type： 杂志文章,评审
doi：10.1016/j.ceb.2019.12.008
更新日期：2020-04-01 00:00:00
abstract：:The insulin receptor substrates function at the heart of the insulin signalling network. It has recently become apparent that the intracellular localisation of these molecules is regulated in a precise manner that is critical for both the generation and the termination of the insulin signal. Some insulin receptor subs...
journal_title：Current opinion in cell biology
pub_type： 杂志文章,评审
doi：10.1016/s0955-0674(99)00079-4
更新日期：2000-04-01 00:00:00
abstract：:Cohesin is a four subunit complex, conserved from yeast to man, with the ability to hold together two DNA segments within its ring-shaped structure. When the two segments belong to sister chromatids, cohesin is mediating cohesion, which is essential for chromosome segregation in mitosis and meiosis and for homologous ...
journal_title：Current opinion in cell biology
pub_type： 杂志文章,评审
doi：10.1016/j.ceb.2012.10.013
更新日期：2013-02-01 00:00:00
abstract：:Kinases belonging to the mitogen-activated protein kinase (MAPK) family are used throughout evolution to control the cellular responses to external signals such as growth factors, nutrient status, stress or inductive signals. Many important substrates for MAPKs are transcription factors, and both the genetic and the b...
journal_title：Current opinion in cell biology
pub_type： 杂志文章,评审
doi：10.1016/s0955-0674(96)80067-6
更新日期：1996-04-01 00:00:00
abstract：:Branching morphogenesis is a widely used strategy to increase the surface area of a given organ. A number of tissues undergo branching morphogenesis during development, including the lung, kidney, vascular system and numerous glands. Until recently, very little has been known about the genetic principles underlying th...
journal_title：Current opinion in cell biology
pub_type： 杂志文章,评审
doi：10.1016/s0955-0674(00)00160-5
更新日期：2000-12-01 00:00:00
abstract：:Actin filaments, microtubules, and intermediate filaments, have all been found to be dynamic structures in living cells. Recent studies have shed important light on the assembly, disassembly, and mobility of these structures. In addition, a growing emphasis has been placed on the regulation of cytoskeletal activities ...
journal_title：Current opinion in cell biology
pub_type： 杂志文章,评审
doi：10.1016/0955-0674(91)90162-r
更新日期：1991-02-01 00:00:00
abstract：:Three recent reports indicate that p120-catenin can modulate the activities of RhoA, Rac and Cdc42, suggesting an elegant and previously unexpected mechanism for regulating the balance between adhesive and motile cellular phenotypes. The observations in these reports provide important new clues toward p120's mechanism...
journal_title：Current opinion in cell biology
pub_type： 杂志文章,评审
doi：10.1016/s0955-0674(00)00258-1
更新日期：2001-10-01 00:00:00
