当前位置： SCI文献检索 > OPHTHALMOLOGY期刊下所有文献 > Immunocytochemical diagnosis as inflammation by vitrectomy cell blocks in patients with vitreous opacity.

Immunocytochemical diagnosis as inflammation by vitrectomy cell blocks in patients with vitreous opacity.

Abstract：

PURPOSE:To describe the clinical and cytopathologic characteristics in patients with vitreous opacity of unknown cause or preceding inflammation, diagnosed cytopathologically as inflammation. DESIGN:Retrospective case series. PARTICIPANTS:Forty-three consecutive patients (61 eyes) who underwent vitrectomy for vitreous opacity of unknown cause or preceding inflammation and were diagnosed cytopathologically with inflammation at one institution in 6 years from 2005 to 2010. During the same period, 11 consecutive patients with vitreous opacity of unknown cause were diagnosed cytopathologically with lymphoma (large B-cell lymphoma) and were excluded from the study. METHODS:Cell blocks were made by centrifugation of vitrectomy fluid and embedded in paraffin for immunocytochemistry. MAIN OUTCOME MEASURES:Cytopathologic and immunocytochemical diagnosis using vitrectomy cell blocks. RESULTS:Histiocytes (macrophages), small lymphocytes, neutrophils, and eosinophils were predominant cells, with no atypical large cells on hematoxylin-eosin staining. Immunocytochemically, most predominant cells were CD68-positive histiocytes (macrophages), followed by CD3-positive T cells, but CD20- or CD79a-positive B cells were rarely present. Epithelioid cells, positive for CD68, were found in 4 patients with or without an established diagnosis of sarcoidosis, and giant multinucleated cells were found in 2 patients with suspected preceding self-limiting Vogt-Koyanagi-Harada disease, based on the presence of depigmented red fundi. Inflammation was diagnosed in 2 patients with vitreous opacity who had a preceding onset of brain lymphoma or systemic lymphoma. CONCLUSIONS:The presence of macrophages, combined with small T lymphocytes, was a major sign in intravitreal inflammation, manifesting as vitreous opacity. A simple technique of cytopathology and immunocytochemistry, using vitrectomy cell blocks, can be performed in most pathology laboratories.

journal_name

Ophthalmology

journal_title

Ophthalmology

authors

Matsuo T,Ichimura K

doi

10.1016/j.ophtha.2011.10.020

subject

Has Abstract

pub_date

2012-04-01 00:00:00

pages

827-37

issue

eissn

0161-6420

issn

1549-4713

pii

S0161-6420(11)00985-7

journal_volume

119

pub_type

杂志文章

在线工具

Immunocytochemical diagnosis as inflammation by vitrectomy cell blocks in patients with vitreous opacity.