Altered profile of seminal plasma microRNAs in the molecular diagnosis of male infertility.

abstract：BACKGROUND:KLK5 is a newly discovered human kallikrein gene. Many kallikrein genes have been found to be differentially expressed in various malignancies, and prostate-specific antigen (PSA; encoded by the KLK3 gene) is the best tumor marker for prostate cancer. Like the genes that encode PSA and other kallikreins, the...

journal_title：Clinical chemistry

authors： Yousef GM,Scorilas A,Kyriakopoulou LG,Rendl L,Diamandis M,Ponzone R,Biglia N,Giai M,Roagna R,Sismondi P,Diamandis EP

更新日期：2002-08-01 00:00:00

abstract：:In this assay of tamoxifen and four metabolites in human serum, the serum samples are deproteinized with an equal volume of acetonitrile, then injected into a small (0.21 X 2 cm) precolumn packed with 5-micron-diameter octadecylsilane (ODS) particles. The samples are concentrated on-column by equilibrating the column ...

journal_title：Clinical chemistry

authors： Lien EA,Ueland PM,Solheim E,Kvinnsland S

更新日期：1987-09-01 00:00:00

abstract：:We describe a modification of the MacGee method [Anal. Chem. 42, 421 (1970)] for rapid determination of phenytoin (diphenylhydantoin) in plasma by gas-liquid chromatography, in which an ethylaing reagent is used instead of the more common methylating reagents. With this modification, several N-methylated antiepileptic...

journal_title：Clinical chemistry

authors： Friel P,Troupin AS

更新日期：1975-05-01 00:00:00

abstract：:Fast lipoprotein chromatography (FLPC) is a novel method for quantifying lipoproteins. Plasma proteins are separated by fast-flow gel filtration. Lipoproteins are detected by post-column derivatization with an enzymatic cholesterol reagent. FLPC resolves very-low-, low-, and high-density lipoproteins (VLDL, LDL, and H...

journal_title：Clinical chemistry

authors： März W,Siekmeier R,Scharnagl H,Seiffert UB,Gross W

更新日期：1993-11-01 00:00:00

abstract：BACKGROUND:Δ(9)-Tetrahydrocannabinol (THC), 11-nor-9-carboxy-THC (THCCOOH), and cannabinol (CBN) were measured in breath following controlled cannabis smoking to characterize the time course and windows of detection of breath cannabinoids. METHODS:Exhaled breath was collected from chronic (≥4 times per week) and occas...

journal_title：Clinical chemistry

authors： Himes SK,Scheidweiler KB,Beck O,Gorelick DA,Desrosiers NA,Huestis MA

更新日期：2013-12-01 00:00:00

abstract：BACKGROUND:The emergence of a novel pandemic human strain of influenza A (H1N1/09) has clearly demonstrated the need for flexible tools enabling the rapid development of new diagnostic methods. METHODS:We designed a set of reverse-transcription quantitative real-time PCR (RT-qPCR) assays based on the Universal ProbeLi...

journal_title：Clinical chemistry

authors： Wenzel JJ,Walch H,Bollwein M,Niller HH,Ankenbauer W,Mauritz R,Höltke HJ,Zepeda HM,Wolf H,Jilg W,Reischl U

更新日期：2009-12-01 00:00:00

abstract：:We followed the "abbreviated precision protocol" of the National Committee for Clinical Laboratory Standards for the evaluation of precision, accuracy, and carryover in analyses for glucose with the "Ektachem." We analyzed 760 clinical samples by this technique, by the FDA Proposed Class Standard glucose reference met...

journal_title：Clinical chemistry

authors： Bandi ZL,Fuller JB,Bee DE,James GP

更新日期：1981-01-01 00:00:00

abstract：BACKGROUND:Urinary cross-linked N-telopeptide of type I collagen (NTX) is a reliable bone resorption marker in patients with metabolic bone disease. We assessed a clinically available serum NTX assay suitable for anuric patients on hemodialysis (HD). METHODS:Serum concentrations of NTX, C-terminal telopeptide of type ...

journal_title：Clinical chemistry

authors： Maeno Y,Inaba M,Okuno S,Yamakawa T,Ishimura E,Nishizawa Y

更新日期：2005-12-01 00:00:00

abstract：BACKGROUND:We used a difference in bias approach to evaluate the commutability of 4 frozen serum pools for 8 direct methods for measurement of HDL and LDL cholesterol (HDLC and LDLC). METHODS:Freshly collected nonfrozen sera from 138 diseased and 37 nondiseased patients and 4 frozen pools from the CDC Lipid Standardiz...

journal_title：Clinical chemistry

authors： Korzun WJ,Nilsson G,Bachmann LM,Myers GL,Sakurabayashi I,Nakajima K,Nakamura M,Shamburek RD,Remaley AT,Miller WG

更新日期：2015-08-01 00:00:00

abstract：:A total of 1357 polypeptides [946 acidic (isoelectric focusing) and 411 basic (nonequilibrium pH-gradient electrophoresis)] from human HeLa cells have been separated and catalogued with use of high-resolution two-dimensional gel electrophoresis. Of these polypeptides, 1266 were detected by labeling cells with [35S]met...

journal_title：Clinical chemistry

authors： Bravo R,Celis JE

更新日期：1982-04-01 00:00:00

abstract：BACKGROUND:A substantial proportion of the variability in the absorption and clearance of cyclosporin A (CsA) after oral administration has been attributed to variability in liver cytochrome P-450 3A4 (CYP3A4) activity and intestinal P-glycoprotein (P-gp) concentration. A polymorphism in the CYP3A4 promoter region, ter...

journal_title：Clinical chemistry

authors： von Ahsen N,Richter M,Grupp C,Ringe B,Oellerich M,Armstrong VW

更新日期：2001-06-01 00:00:00

abstract：BACKGROUND:The resurgence of the parasitic disease schistosomiasis calls for more efficient diagnostic tests. We developed a rapid, simple, portable, and sensitive immunoagglutination assay that uses SiO(2) particles and quartz crystal microbalance (QCM) for quantifying Schistosoma japonicum (Sj) antibodies (SjAb). ME...

journal_title：Clinical chemistry

authors： Wang H,Zhang Y,Yan B,Liu L,Wang S,Shen G,Yu R

更新日期：2006-11-01 00:00:00

abstract：BACKGROUND:To determine the contribution of laboratory costs to the overall costs of managing hospital patients with different diseases, we studied the costs of laboratory testing overall and in relation to the other costs incurred during hospitalization. METHODS:We used a database developed by the University HealthSy...

journal_title：Clinical chemistry

authors： Young DS,Sachais BS,Jefferies LC

更新日期：2000-07-01 00:00:00

abstract：:We have developed a liquid chromatography-isotope dilution mass spectrometry procedure to quantify total cholesterol in serum. A particle-beam interface was used for coupling the liquid chromatograph and the mass spectrometer. After electron impact ionization the ions m/z = 386 and m/z = 389 were used for selective io...

journal_title：Clinical chemistry

authors： Kock R,Delvoux B,Greiling H

更新日期：1997-10-01 00:00:00

abstract：:A triclonal gammopathy is an immunoglobulin abnormality in which three discrete monoclonal subpopulations of immunoglobulin molecules are present in a patient's serum. Monoclonal and biclonal gammopathies have been studied extensively, but relatively little is known about the much rarer triclonal gammopathies. The cas...

journal_title：Clinical chemistry

authors： Ray RA,Schotters SB,Jacobs A,Rodgerson DO

更新日期：1986-01-01 00:00:00

abstract：:A liquid-chromatographic technique with a post-column enzymatic reaction and fluorescence detection was validated for analysis of individual bile acids in the serum of rats. Extraction recoveries averaged 91.1% (SD 6.9%) for all bile acids. The assay was sensitive (minimum detection of 16.8 pmol per 100-microL injecti...

journal_title：Clinical chemistry

authors： Thompson MB,Blair PC,Morris RW,Neptun DA,Deyo DF,Popp JA

更新日期：1987-10-01 00:00:00

abstract：:Throughout the 1970s, controversy centered both on immunoassay "sensitivity" per se and on the relative sensitivities of labeled antibody (Ab) and labeled analyte methods. Our theoretical studies revealed that RIA sensitivities could be surpassed only by the use of very high-specificity nonisotopic labels in "noncompe...

journal_title：Clinical chemistry

authors： Ekins RP,Chu FW

更新日期：1991-11-01 00:00:00

abstract：BACKGROUND:Laboratory tests can be an important source of blood loss in hospitals, especially for newborns and patients in intensive care. The aim of this study was to quantify blood loss for laboratory diagnostic tests in a large number of patients in a teaching hospital. METHODS:We estimated blood loss by multiplyin...

journal_title：Clinical chemistry

authors： Wisser D,van Ackern K,Knoll E,Wisser H,Bertsch T

更新日期：2003-10-01 00:00:00

abstract：BACKGROUND:Consideration of circulating biomarkers for risk stratification in heart failure (HF) is recommended, but the influence of atrial fibrillation (AF) on prognostic performance of many markers is unclear. We investigated the influence of AF on the prognostic performance of circulating biomarkers in HF. METHODS...

journal_title：Clinical chemistry

authors： Tan ESJ,Chan SP,Liew OW,Chong JPC,Leong GKT,Yeo DPS,Ong HY,Jaufeerally F,Yap J,Sim D,Ng TP,Ling LH,Lam CSP,Richards AM

更新日期：2021-01-08 00:00:00

abstract：BACKGROUND:Late enhancement MRI (LE-MRI) and cardiac troponin I (cTnI) are sensitive methods to detect subclinical myocardial injury. We sought to investigate the relation between plasma concentrations of cTnI measured with a high-sensitivity assay (hs-cTnI) and the development of unrecognized myocardial infarctions (U...

journal_title：Clinical chemistry

authors： Barbier CE,Themudo R,Bjerner T,Johansson L,Lindahl B,Venge P,Lind L,Ahlström H

更新日期：2014-10-01 00:00:00

abstract：:Lactate dehydrogenase (LD) isoenzymes 1 and 2 in human serum were separated on a column of diethylaminoethyl-Sephadex. Samples layered on mini-columns were eluted with buffered sodium chloride (100, 150, and 200 mmol/liter). Lactate dehydrogenase activity in column effluents was measured by the Wacker method, and thei...

journal_title：Clinical chemistry

authors： Mercer DW

更新日期：1978-03-01 00:00:00

abstract：BACKGROUND:It has been postulated that changes in S-adenosylhomocysteine (AdoHcy), a potent inhibitor of transmethylation, provide a mechanism by which increased homocysteine causes its detrimental effects. We aimed to develop a rapid and sensitive method to measure AdoHcy and its precursor S-adenosylmethionine (AdoMet...

journal_title：Clinical chemistry

authors： Gellekink H,van Oppenraaij-Emmerzaal D,van Rooij A,Struys EA,den Heijer M,Blom HJ

更新日期：2005-08-01 00:00:00

abstract：BACKGROUND:Multiple reaction monitoring mass spectrometry (MRM-MS) of peptides with stable isotope-labeled internal standards (SISs) is increasingly being used to develop quantitative assays for proteins in complex biological matrices. These assays can be highly precise and quantitative, but the frequent occurrence of ...

journal_title：Clinical chemistry

authors： Abbatiello SE,Mani DR,Keshishian H,Carr SA

更新日期：2010-02-01 00:00:00

abstract：:In the present clinical evaluation of Triage immunoassay for detection of drugs of abuse, we examined a possible connection between the laboratory skills of the personnel using it and the quality of analytical results; we also evaluated the degree of concordance between the Triage results and those obtained by an inst...

journal_title：Clinical chemistry

authors： de la Torre R,Domingo-Salvany A,Badia R,Gonzàlez G,McFarlane D,San L,Torrens M

更新日期：1996-09-01 00:00:00

abstract：BACKGROUND:PCR-based detection of minimal residual disease (MRD) in neuroblastoma (NB) patients can be used for initial staging and monitoring therapy response in bone marrow (BM) and peripheral blood (PB). PHOX2B has been identified as a sensitive and specific MRD marker; however, its expression varies between tumors....

journal_title：Clinical chemistry

authors： Stutterheim J,Gerritsen A,Zappeij-Kannegieter L,Yalcin B,Dee R,van Noesel MM,Berthold F,Versteeg R,Caron HN,van der Schoot CE,Tytgat GA

更新日期：2009-07-01 00:00:00

abstract：BACKGROUND:Reports of the use of multiplex enzyme assay screening for Pompe disease, Fabry disease, Gaucher disease, Niemann-Pick disease types A and B, and Krabbe disease have engendered interest in the use of this assay in newborn screening. We modified the assay for high-throughput use in screening laboratories. ME...

journal_title：Clinical chemistry

authors： Zhang XK,Elbin CS,Chuang WL,Cooper SK,Marashio CA,Beauregard C,Keutzer JM

更新日期：2008-10-01 00:00:00

abstract：BACKGROUND:The optimal situation in external quality assessment (EQA) is to use commutable materials. No previous study has examined the commutability of a whole-blood material for point-of-care (POC) testing. The aim of this study was to determine the commutability of the Norwegian Quality Improvement of Laboratory Ex...

journal_title：Clinical chemistry

authors： Bukve T,Sandberg S,Vie WS,Sølvik U,Christensen NG,Stavelin A

更新日期：2019-06-01 00:00:00

abstract：:To determine when a change in serial measurements of prothrombin time in patients receiving oral anticoagulant therapy (ACT) is a statistically significant biological change necessitating dose adjustment, one must know the size of the "critical difference" in statistical terms (i.e., probabilities). In a cohort of 32 ...

journal_title：Clinical chemistry

authors： Lassen JF,Brandslund I,Antonsen S

更新日期：1995-03-01 00:00:00

abstract：:This communication describes and evaluates an improved routine methodology for quantitating clinical proteinuria. Based on investigations of Piscator and of Savory et al., a modified Tsuchiya's reagent (ethanolic HCI-phosphotungstic acid) is used to precipitate proteins at 56 degrees C, followed by biuret spectrophoto...

journal_title：Clinical chemistry

authors： Rice EW

更新日期：1975-03-01 00:00:00

abstract：:The technology needed to genetically engineer antibodies is evolving rapidly and the potential utility of these novel reagents is being explored with vigor. The process includes cloning of the antibody genes, their in vitro manipulation and mutagenesis, expression in a suitable host/vector system, and, for commercial ...

journal_title：Clinical chemistry

authors： Moore GP

更新日期：1989-09-01 00:00:00