Aromatase expression in Xenopus oocytes: a three cell-type model for the ovarian estradiol synthesis.

abstract：:Inhibition of aromatase, a key enzyme in the biosynthesis of oestradiol-17 beta, by the addition of 1,4,6-androstatrien-3,17-dione resulted in a significant increase in the levels of immunoreactive human chorionic gonadotrophin (hCG) in the medium and tissue. This increase was partially reversed by the simultaneous ad...

journal_title：Journal of molecular endocrinology

authors： Sharma SC,Purohit P,Rao AJ

更新日期：1993-08-01 00:00:00

abstract：:CRH and its structurally related peptide urocortin (Ucn) are released under stress. Ucn is a potent agonist for CRH-receptor 2 (CRH-R2), which is strongly expressed in rodent heart. Stress induces Ucn mRNA expression in the heart, where it may be cardioprotective. However, increasing evidence indicates that Ucn may al...

journal_title：Journal of molecular endocrinology

authors： Huang M,Kempuraj D,Papadopoulou N,Kourelis T,Donelan J,Manola A,Theoharides TC

更新日期：2009-05-01 00:00:00

abstract：:Seven bovine follistatin (FS) cDNA clones were isolated from a bovine ovarian follicle cDNA library. The predicted amino acid sequences revealed that six of the cDNA clones represented an FS precursor of 344 amino acids which corresponded to a mature FS of 315 amino acids (FS 315), with one cDNA clone containing the e...

journal_title：Journal of molecular endocrinology

authors： Saleh M,Garcia S,Mercer JE,Findlay JK

更新日期：1994-12-01 00:00:00

abstract：:Whether insulin, at physiological concentrations, has direct effects on vascular smooth muscle cells (VSMCs) remains controversial. Our aim was to characterize the mechanism for insulin resistance in VSMCs. For comparison, the effects of IGF1 and IGF2 were also studied. Cultured human aortic smooth muscle cells (HASMC...

journal_title：Journal of molecular endocrinology

authors： Chisalita SI,Johansson GS,Liefvendahl E,Bäck K,Arnqvist HJ

更新日期：2009-12-01 00:00:00

abstract：:cDNA clones coding for rabbit prolactin were isolated from a pituitary library using a rat prolactin RNA probe. One cDNA contained 873 bases including the entire coding sequence of rabbit prolactin, its signal peptide and the 5' and 3' untranslated regions of 44 and 145 nucleotides respectively. The deduced amino acid...

journal_title：Journal of molecular endocrinology

authors： Gabou L,Boisnard M,Gourdou I,Jammes H,Dulor JP,Djiane J

更新日期：1996-02-01 00:00:00

abstract：:The GnRH receptor (GnRHR) is expressed in several non-pituitary tissues, notably in gonads. However, mechanisms underlying the gonad-specific expression of Gnrhr are not well understood. Here, Gnrhr expression was analysed in the developing testes and pituitaries of rats and transgenic mice bearing the human placental...

journal_title：Journal of molecular endocrinology

authors： Ishaq M,Schang AL,Magre S,Laverrière JN,Guillou A,Coudouel N,Wargnier R,Cohen-Tannoudji J,Counis R

更新日期：2013-04-23 00:00:00

abstract：:The chemokine Stromal-derived factor-1alpha (SDF-1alpha) interacts with seven transmembrane (TM) G-protein-coupled receptor (GPR), CXCR4. SDF-1alpha is linked to inflammation, chemoattraction, cancer metastasis, and hematopoiesis. Tachykinin (Tac1) peptides bind seven transmembrane (TM), GPR and are involved in tumor ...

journal_title：Journal of molecular endocrinology

authors： Corcoran KE,Malhotra A,Molina CA,Rameshwar P

更新日期：2008-03-01 00:00:00

abstract：:Sucrose density gradient purified plasma membranes isolated from brown adipose tissue of cold-acclimated hamsters (4-10 weeks at 0-4 degreesC) were analysed for the content of the short (GsalphaS) and long (GsalphaL) variants of Gsalpha protein (the alpha subunit of the stimulatory G protein) and compared with the mem...

journal_title：Journal of molecular endocrinology

authors： Bourová L,Novotn J,Svoboda P

更新日期：1999-02-01 00:00:00

abstract：:Prolactin (PRL)-releasing peptide (PrRP) is a strong candidate stimulator of pituitary PRL transcription and secretion in teleosts. However, the role in control of extrapituitary PRL expression is unclear even in mammals. To study the possible presence of PrRP-PRL axes not only in the brain-pituitary but also in perip...

journal_title：Journal of molecular endocrinology

authors： Sakamoto T,Amano M,Hyodo S,Moriyama S,Takahashi A,Kawauchi H,Ando M

更新日期：2005-06-01 00:00:00

abstract：:During the process of insulitis in the pathogenesis of type I (insulin-dependent) diabetes mellitus, proinflammatory cytokines induce expression of the death receptor Fas on the surface of pancreatic beta-cells and thereby contribute to the enhanced susceptibility of beta-cells for apoptosis. The aim of this study was...

journal_title：Journal of molecular endocrinology

authors： Augstein P,Wachlin G,Berg S,Bahr J,Salzsieder C,Hehmke B,Heinke P,Salzsieder E

更新日期：2003-04-01 00:00:00

abstract：:Translocation of protein kinase C (PKC) from the cytosol to the plasma membranes is believed to reflect activation of the enzyme. We have studied translocation of PKC in lactotroph-enriched anterior pituitary cell cultures by measuring the incorporation of gamma-32P from [gamma-32P]ATP into a synthetic peptide substra...

journal_title：Journal of molecular endocrinology

authors： Mau SE,Saermark T,Vilhardt H

更新日期：1994-06-01 00:00:00

abstract：:Porcine prolactin cDNA clones were screened using antiserum against ovine prolactin from a cDNA library of porcine anterior pituitary, and their nucleotide sequences were determined by the chain-termination method. The nucleotide sequence of the 5' untranslated region and part of the signal peptide region were determi...

journal_title：Journal of molecular endocrinology

authors： Kato Y,Hirai T,Kato T

更新日期：1990-04-01 00:00:00

abstract：:In previous studies we demonstrated that IGF-I induces proliferation of pituitary lactotrophs. In addition to its mitotrophic actions, IGF-I is known to prevent apoptosis induced by diverse stimuli in several cell types. In this study, we investigated the action of IGF-I on pituitary cell survival and the intracellula...

journal_title：Journal of molecular endocrinology

authors： Fernández M,Sánchez-Franco F,Palacios N,Sánchez I,Fernández C,Cacicedo L

更新日期：2004-08-01 00:00:00

abstract：:The melanocortin 2 receptor accessory protein (MRAP) was originally discovered to be an essential co-receptor for the ACTH receptor/melanocortin 2 receptor, and it physically interacts with this receptor and is required for receptor trafficking and ligand binding. A related molecule, MRAP2, is mainly expressed in the ...

journal_title：Journal of molecular endocrinology

authors： Clark AJ,Chan LF

更新日期：2017-04-01 00:00:00

abstract：:Tumour necrosis factor superfamily member 11 (TNFSF11) gene, that codes for receptor activator of nuclear factor-kappaB ligand, is one of the candidate genes for the genetic susceptibility to osteoporosis. As variations in the TNFSF11 gene promoter could alter its expression, the aim of the study was to evaluate the f...

journal_title：Journal of molecular endocrinology

authors： Mencej S,Albagha OM,Prezelj J,Kocjan T,Marc J

更新日期：2008-06-01 00:00:00

abstract：:The effect of steroid hormone treatment on coho salmon (Oncorhynchus kisutch) was examined. The cDNAs for coho salmon FSH beta and LH beta subunits were cloned and sequenced using reverse transcriptase PCR. Northern blot analysis revealed that a single transcript of 1 kb for each of these subunits was present in the p...

journal_title：Journal of molecular endocrinology

authors： Dickey JT,Swanson P

更新日期：1998-12-01 00:00:00

abstract：:In vertebrates, sex is determined essentially by two means, genetic factors located on sex chromosomes and epigenetic factors such as temperature experienced by the individual during development. Steroids, especially estrogens, are clearly involved in gonadal differentiation in non-mammalian vertebrates. In this regar...

journal_title：Journal of molecular endocrinology

authors： Kuntz S,Chesnel A,Flament S,Chardard D

更新日期：2004-12-01 00:00:00

abstract：:Retinoic acid (RA) and sodium butyrate (NaB) are regulators of cell growth and differentiation. We studied their effect on normal (SVC1) or v-Ki-ras-transformed (Ki-SVC1) rat seminal vesicle (SV) epithelial cell lines. The treatment of these cells with 10(-((7( M RA did not produce significant changes in the morpholog...

journal_title：Journal of molecular endocrinology

authors： Buommino E,Pasquali D,Sinisi AA,Bellastella A,Morelli F,Metafora S

更新日期：2000-02-01 00:00:00

abstract：:Receptive endometrium is a prerequisite for successful embryo implantation, and it follows that poor endometrial receptivity is a leading cause of implantation failure. miRNAs play important roles as epigenetic regulators of endometrial receptivity and embryo implantation through post-transcriptional modifications. Ho...

journal_title：Journal of molecular endocrinology

authors： Akbar R,Ullah K,Rahman TU,Cheng Y,Pang HY,Jin LY,Wang QJ,Huang HF,Sheng JZ

更新日期：2020-01-01 00:00:00

abstract：:We studied the effects of 2-methoxyestradiol (2-ME2) and 16alpha-hydroxyestrone (16alpha-OHE1), two metabolites of estradiol (E2), on DNA synthesis, cell cycle progression and cyclin D1 protein levels in estrogen receptor-positive MCF-7 cells. E2 and 16alpha-OHE1 stimulated DNA synthesis, and 2-ME2 inhibited the stimu...

journal_title：Journal of molecular endocrinology

authors： Lewis JS,Thomas TJ,Pestell RG,Albanese C,Gallo MA,Thomas T

更新日期：2005-02-01 00:00:00

abstract：:The mannose 6-phosphate/IGF 2 receptor (IGF2R) is comprised of 15 extra-cellular domains that bind IGF2 and mannose 6-phosphate ligands. IGF2R transports ligands from the Golgi to the pre-lysosomal compartment and thereafter to and from the cell surface. IGF2R regulates growth, placental development, tumour suppressio...

journal_title：Journal of molecular endocrinology

authors： Rezgui D,Williams C,Savage SA,Prince SN,Zaccheo OJ,Jones EY,Crump MP,Hassan AB

更新日期：2009-04-01 00:00:00

abstract：:Granulosa cells were prepared from small follicles (less than 3 mm) from the ovaries of 5-month-old gilts. They were cultured in plastic dishes coated with a synthetic adhesion peptide in a chemically defined medium supplemented with 2% serum substitute. After 3 days of culture, the cells reached confluence and expres...

journal_title：Journal of molecular endocrinology

authors： Goxe B,Salesse R,Remy JJ,Genty N,Garnier J

更新日期：1992-04-01 00:00:00

abstract：:Metformin improves obesity-associated metabolic dysregulation, but has controversial effects on adipose tissue inflammation. The objective of the study is to examine the direct effect of metformin on adipocyte inflammatory responses and elucidate the underlying mechanisms. Adipocytes were differentiated from 3T3-L1 ce...

journal_title：Journal of molecular endocrinology

authors： Qi T,Chen Y,Li H,Pei Y,Woo SL,Guo X,Zhao J,Qian X,Awika J,Huo Y,Wu C

更新日期：2017-07-01 00:00:00

abstract：:Human thyroid hormone receptor (c-erb A protein) produced by Escherichia coli expression vector plasmid was purified sequentially using polyethylenimine precipitation of DNA, hydroxylapatite column chromatography, ammonium sulphate precipitation, Sephacryl S-300 gel filtration and mono Q-Sepharose column chromatograph...

journal_title：Journal of molecular endocrinology

authors： Ichikawa K,Hashizume K,Nishii Y,Takeda T,Kobayashi M,Suzuki S,Yamada T

更新日期：1991-10-01 00:00:00

abstract：:Hypothalamic GHRH controls the release of GH from the pituitary gland and also acts as a growth factor in a variety of cancers. The mitogenetic activity of GHRH is exerted through the binding to the pituitary type receptor (pGHRH-R) and its splice variants, mainly SV1. The intracellular signaling pathways that are act...

journal_title：Journal of molecular endocrinology

authors： Barabutis N,Siejka A,Schally AV,Block NL,Cai R,Varga JL

更新日期：2010-02-01 00:00:00

abstract：:The crystal structures of the leucine-rich repeat domain (LRD) of the FSH receptor (FSHR) in complex with FSH and the TSH receptor (TSHR) LRD in complex with the thyroid-stimulating autoantibody (M22) provide opportunities to assess the molecular basis of the specificity of glycoprotein hormone-receptor binding. A com...

journal_title：Journal of molecular endocrinology

authors： Núñez Miguel R,Sanders J,Chirgadze DY,Blundell TL,Furmaniak J,Rees Smith B

更新日期：2008-09-01 00:00:00

abstract：:Testicular Leydig cells (LC) are modulated by several pathways, one of them being the histaminergic system. Heme oxygenase-1 (HO-1), whose upregulation comprises the primary response to oxidative noxae, has a central homeostatic role and might dysregulate LC functions when induced. In this report, we aimed to determin...

journal_title：Journal of molecular endocrinology

authors： Raices T,Varela ML,Monzón CM,Correa Torrado MF,Pagotto RM,Besio Moreno M,Mondillo C,Pignataro OP,Pereyra EN

更新日期：2019-10-01 00:00:00

abstract：:It has recently been shown that 20 kDa human growth hormone (hGH) forms the 1:2 hGH:hGH receptor (hGHR) complex and expresses full agonistic activity, although it hardly forms the 1:1 GH:GHR complex as compared with 22 kDa hGH. To clarify this mechanism, we analyzed the mode of receptor dimerization of 20 kDa hGH usin...

journal_title：Journal of molecular endocrinology

authors： Uchida H,Banba S,Wada M,Matsumoto K,Ikeda M,Naito N,Tanaka E,Honjo M

更新日期：1999-12-01 00:00:00

abstract：:The classical concept of the renin-angiotensin system (RAS) is that of a blood-borne cascade, whose final and bioactive product, angiotensin II, plays an important endocrine role in the maintenance of blood pressure and electrolyte as well as fluid balance. In addition to this circulating RAS, there are an increasing ...

journal_title：Journal of molecular endocrinology

authors： Leung PS,Carlsson PO

更新日期：2001-06-01 00:00:00

abstract：:Fatty acids are essential nutrients that contribute to several intracellular functions. Fatty acid synthesis and oxidation are known to be regulated by sterol regulatory element-binding proteins (SREBPs), which play a pivotal role in the regulation of cellular triglyceride synthesis and cholesterol biogenesis. Recent ...

journal_title：Journal of molecular endocrinology

authors： Park HY,Kang HS,Im SS

更新日期：2018-10-01 00:00:00