Abstract:
:Drug-resistant clones selected from FR3T3 rat cells after transfer of neo-BPV1 (Bovine Papillomavirus Type 1) DNA constructs became phenotypically transformed (focal transformation, growth in suspension and tumor formation) soon after selection (approximately 5 generations in culture). A frameshift mutation in ORF E5 abolished transformation, but did not prevent the autonomous maintenance of the DNA construct. A more complex situation was observed when the E2 transactivating function was abrogated. A minority of the E2(-)-neor clones became phenotypically transformed shortly after drug selection, but the majority maintained normal growth properties for 30 to 50 generations. The rate of viral transcription was uniformly high in cells which exhibited transformed growth properties early after selection (the E2- minority class and all the wild type transformants) and low in phenotypically normal cells (the majority of the E2- lines). The same low transcriptional activity and delayed expression of transformed growth properties had been observed after transfection of a similar construct carrying a wild type viral early region (69-T fragment), but lacking the late region. The elevated rate of viral transcription, which correlates with the immediate expression of transformation, appears therefore to require at least two distinct elements, the E2 transactivator function and sequences in the late region of the viral genome. In their absence, high transcription rates and transformation could be established only in a minority of the transfected clones, by an unknown, E2-independent mechanism. Evidence was obtained for a third transformation route which, in the absence of either E2 or the late region, led to the focal occurrence of transformed derivatives after 30 to 50 generations of normal growth, but was not associated with an overall increase in viral expression.
journal_name
Oncogenejournal_title
Oncogeneauthors
Binétruy B,Schiller J,Lowy D,Cerni C,Cuzin Fsubject
Has Abstractpub_date
1990-11-01 00:00:00pages
1645-51issue
11eissn
0950-9232issn
1476-5594journal_volume
5pub_type
杂志文章相关文献
ONCOGENE文献大全abstract::The REL gene is amplified in many human B-cell lymphomas and we have previously shown that expression of REL from a retroviral vector can malignantly transform chicken spleen cells in vitro. To identify REL protein functions necessary for malignant transformation, we have performed deletion analysis on REL sequences e...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1206801
更新日期:2003-10-09 00:00:00
abstract::The p53 and MDM2 genes are part of a physiological pathway frequently impaired in human cancer. With the exception of tumours occasionally associated with hereditary predisposition, childhood malignancies have not been studied in detail yet. This is the first report on the analysis of p53 and MDM2 in a group of non-he...
journal_title:Oncogene
pub_type: 杂志文章
doi:
更新日期:1993-10-01 00:00:00
abstract::Hypoxia induces transcription of a range of physiologically important genes including erythropoietin and vascular endothelial growth factor. The transcriptional activation is mediated by the hypoxia-inducible factor-1 (HIF-1), a heterodimeric member of the basic helix-loop-helix PAS family, composed of alpha and beta ...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1203938
更新日期:2000-11-16 00:00:00
abstract::Immunoreceptor tyrosine-based activation motifs (ITAMs) are involved in the transduction of signals necessary for activation, differentiation, and survival in hematopoietic cells. Several viruses have been shown to encode ITAM-containing transmembrane proteins. Although expression of these viral proteins has in some c...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1209296
更新日期:2006-05-04 00:00:00
abstract::Mechanisms underlying multidrug resistance (MDR), one of the major causes of cancer treatment failure, are still poorly understood. We selected the osteosarcoma MDR HosDXR150 cell line by culturing Hos cells in the presence of increasing doxorubicin doses and showed that it is crossresistant to vinblastine. Similarly ...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1206487
更新日期:2003-06-05 00:00:00
abstract::Ultraviolet light (UV) induced DNA lesions efficiently block transcript elongation and induce the p53 response. Although p53 contributes to transcriptional activation of the p21waf1 and bax genes, accumulation of these proteins requires that these genes are free of UV induced pyrimidine dimers. We assessed the level o...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1201963
更新日期:1998-08-06 00:00:00
abstract::The maintenance cytosine DNA methyltransferase DNMT1 and de novo methyltransferase DNMT3b cooperate to establish aberrant DNA methylation and chromatin complexes to repress gene transcription during cancer development. The expression of DNMT3b was constitutively increased 5-20-fold in hTERT/CDK4-immortalized human bro...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/onc.2013.580
更新日期:2015-01-29 00:00:00
abstract::Prostate tumors develop resistance to androgen deprivation therapy (ADT) by multiple mechanisms, one of which is to express constitutively active androgen receptor (AR) splice variants lacking the ligand-binding domain. AR splice variant 7 (AR-V7, also termed AR3) is the most abundantly expressed variant that drives p...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/onc.2013.284
更新日期:2014-06-12 00:00:00
abstract::Cancer is classically considered as a genetic and, more recently, epigenetic multistep disease. Despite seminal studies in the 1920s by Warburg showing a characteristic metabolic pattern for tumors, cancer bioenergetics has often been relegated to the backwaters of cancer biology. This review aims to provide a histori...
journal_title:Oncogene
pub_type: 历史文章,杂志文章,评审
doi:10.1038/onc.2011.576
更新日期:2012-09-06 00:00:00
abstract::Metastatic growth in breast cancer (BC) has been proposed as an exclusive property of cancer stem cells (CSCs). However, formal proof of their identity as cells of origin of recurrences at distant sites and the molecular events that may contribute to tumor cell dissemination and metastasis development are yet to be el...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/onc.2014.5
更新日期:2015-02-05 00:00:00
abstract::A lot of effort has been done to study how cancer cells react to low-oxygen tension, a condition known as hypoxia. Indeed, abnormal and dysfunctional blood vessels in the tumor are incapable to restore oxygenation, therefore perpetuating hypoxia, which, in turn, will fuel tumor progression, metastasis and resistance t...
journal_title:Oncogene
pub_type: 杂志文章,评审
doi:10.1038/onc.2013.121
更新日期:2014-04-03 00:00:00
abstract::Ectopic expression of metabotropic glutamate receptor subtype 1 (mGluR1) in mouse melanocytes induces melanoma formation. Although requirement of mGluR1 for development of melanoma in the initial stage has been demonstrated, its role in melanoma growth in vivo remains unclear. In this study, we developed novel transge...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/onc.2008.329
更新日期:2008-12-04 00:00:00
abstract::To understand the mechanism for resistance of primary cultures of rat embryo fibroblasts (REFs) to oncogene-induced transformation, we studied the transforming ability of a recombinant retrovirus, ZSV, containing v-src and neo genes in REFs and in the rat cell line F2408. The susceptibility of REFs to p60v-src transfo...
journal_title:Oncogene
pub_type: 杂志文章
doi:
更新日期:1995-07-20 00:00:00
abstract::Appropriate expression of HTLV-1 genes requires transcriptional transactivation by Tax and post-transcriptional regulation by Rex, both mediated by LTR encoded RNA sequences. Using a combination of deletion mutagenesis, Rex-reporter CAT assays, fluorescence in situ hybridization (FISH) and confocal laser scanning micr...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1201884
更新日期:1998-06-25 00:00:00
abstract::Stimulation of resting W53 cells (lymphoid murine cells expressing prolactin (PRL) receptor) by PRL induced expression of growth-related immediate-early genes (IEG), and proliferation through activation of the Src kinases. Since IEG are essential for cell cycle progression, we have studied how PRL controls expression ...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1208002
更新日期:2004-09-23 00:00:00
abstract::Tat protein is an early nonstructural protein necessary for virus replication, which is secreted by infected cells and taken up by uninfected cells. Extensive evidence indicates that Tat may be a cofactor in the development of AIDS-related neoplasms. The molecular mechanism underlying Tat's oncogenic activity may incl...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1206637
更新日期:2003-09-18 00:00:00
abstract::The Cbl family proteins Cbl, Cbl-b, and Cbl-c/Cbl-3 are thought to regulate signaling through protein-tyrosine kinases, positively as scaffold proteins and negatively as ubiquitin ligases. However, the precise signaling pathways and target proteins for each Cbl family member are not well understood. Here we show that ...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1207298
更新日期:2004-03-04 00:00:00
abstract::Apoptin, a small protein from the chicken anemia virus, has attracted attention because of its specificity in killing tumor cells. Localization of apoptin in the nucleus of tumor cells has been shown to be vital for proapoptotic activity, however, targeted expression of apoptin in the nucleus of normal cells does not ...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1210958
更新日期:2008-05-08 00:00:00
abstract::Several small GTPases of the Ras superfamily have been shown to antagonize TGFbeta signaling in human tumor cell lines. Some of these GTPases are post-translationally modified by farnesylation, a lipid modification catalyzed by farnesyltransferase and required for the proteins to attach to membranes and to function. I...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1203920
更新日期:2000-11-16 00:00:00
abstract::Rac1-GTPases serve as intermediary cellular switches, which conduct transient and constitutive signals from upstream cues, including those from Ras oncoproteins. Although the sirtuin1 (SIRT1) deacetylase is overexpressed in several human cancers and has recently been linked to cancer cell motility as a context-depende...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/onc.2013.549
更新日期:2015-01-08 00:00:00
abstract::Persistent infection with hepatitis B virus (HBV) is one of the primary risk factors for human hepatocellular carcinoma (HCC). In a human ecological study, we have shown that, in addition to HBV, animal food consumption also significantly contributes to the variance of HCC. To test the interacting effect of HBV and an...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1201444
更新日期:1997-12-04 00:00:00
abstract::The forkhead transcription factor FOXM1 has a key role in DNA damage response, and its deregulated overexpression is associated with genotoxic drug resistance in breast cancer. However, little is known about the posttranslational mechanisms by which FOXM1 expression is regulated by genotoxic agents and how they are de...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/onc.2015.208
更新日期:2016-03-17 00:00:00
abstract::Malignant mesotheliomas (MMs) are very aggressive tumors that respond poorly to standard chemotherapeutic approaches. The phosphatidylinositol 3-kinase (PI3K)/AKT pathway has been implicated in tumor aggressiveness, in part by mediating cell survival and reducing sensitivity to chemotherapy. Using antibodies recognizi...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1208744
更新日期:2005-09-08 00:00:00
abstract::Histone deacetylase inhibitors (HDACis) are a promising class of anticancer epigenetic drugs, however, molecular factors influencing the responses of individual tumors to HDACi therapies remain obscure. Here, we sought to identify genes associated with HDACi resistance in gastric cancer. Treating a panel of 17 gastric...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/onc.2013.104
更新日期:2014-03-20 00:00:00
abstract::Targeting Bruton tyrosine kinase (BTK) by ibrutinib is an effective treatment for patients with relapsed/refractory mantle cell lymphoma (MCL). However, both primary and acquired resistance to ibrutinib have developed in a significant number of these patients. A combinatory strategy targeting multiple oncogenic pathwa...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/onc.2016.155
更新日期:2016-12-01 00:00:00
abstract::Tuftelin1 (TUFT1), an acidic protein constituent of developing and mineralizing tooth tissues, is regulated by hypoxia and the Hedgehog signaling pathway. We investigated the role of TUFT1 in hepatocellular carcinoma (HCC). qRT-PCR, immunohistochemistry and western blot were employed to evaluate TUFT1 level in HCC. MT...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/s41388-018-0505-8
更新日期:2019-02-01 00:00:00
abstract::The transcriptional activity of the androgen receptor (AR) is regulated by both ligand binding and post-translational modifications, including acetylation and small ubiquitin-like modifier (SUMO)ylation. Histone deacetylases (HDACs) are known to catalyze the removal of acetyl groups from both histones and non-histone ...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/onc.2010.600
更新日期:2011-05-12 00:00:00
abstract::Osteoclasts are multinuclear bone-resorbing cells that differentiate from hematopoietic precursor cells. Prostate cancer cells frequently spread to bone and secrete soluble signaling factors to accelerate osteoclast differentiation and bone resorption. However, processes and mechanisms that govern the expression of os...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/s41388-018-0356-3
更新日期:2018-10-01 00:00:00
abstract::The ability of tumour cells to resist apoptosis-inducing signals by cytotoxic T cells may decide the success or failure of tumour elimination. An important effector of apoptosis is the CD95/CD95 ligand system (APO-1/Fas) that mediates perforin-independent cytotoxic T-cell killing of tumour cells. We propose a new stra...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1207387
更新日期:2004-04-29 00:00:00
abstract::Stimulation of the c-Kit receptor tyrosine kinase has a critical role in the development and migration of melanocytes, and oncogenic c-Kit mutants contribute to the progression of some melanomas. c-Kit signalling activates the mitogen-activated protein kinase (MAPK) and phosphatidylinositol 3-kinase (PI3K) pathways an...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/onc.2012.562
更新日期:2014-01-09 00:00:00