Abstract:
:Current vaccine candidates against Rift Valley fever virus (RVFV) incorporate the viral structural glycoproteins as antigens, since triggering antibody responses against them usually correlates with protection. Here, we have focused solely on the nucleoprotein of RVFV as a potential target for vaccine development. Previous studies in mouse models have already demonstrated that RVFV nucleoprotein can elicit partial protection when administered by means of a DNA vaccine or in recombinant, soluble, protein form. To determine whether this partially protective immune response could be augmented to a level comparable to DNA constructs encoding for RVFV glycoproteins, several targeting sequences were cloned adjacent to the RVFV nucleoprotein (N) gene. Immunization with a plasmid construct encoding for a ubiquitinated form of the viral nucleoprotein (pCMV-Ub-N) significantly increased the survival of IFNAR(-/-) mice following viral challenge to levels comparable with a recombinant DNA-vaccine encoding both RVFV glycoproteins. Mice immunized with pCMV-Ub-N also displayed higher levels of non-neutralizing anti-N antibodies and antigen-specific T-cell responses. This suggests a role for other cell mediated responses in protection against RVFV. These findings show the potential of RVFV N as a candidate antigen for vaccination, and present a new strategy in vaccine design against certain bunyaviruses, where glycoprotein variation may impede effective broad-based vaccination strategies.
journal_name
Vaccinejournal_title
Vaccineauthors
Boshra H,Lorenzo G,Rodriguez F,Brun Adoi
10.1016/j.vaccine.2011.04.043subject
Has Abstractpub_date
2011-06-15 00:00:00pages
4469-75issue
27eissn
0264-410Xissn
1873-2518pii
S0264-410X(11)00588-3journal_volume
29pub_type
杂志文章相关文献
VACCINE文献大全abstract::Bacterial flagellin is known to induce potent immune response in vertebrate systems via the toll-like receptor (TLR) 5. As a result, flagellin has been studied extensively as a vaccine adjuvant. In a previous study, we examined the vaccine and adjuvant potentials of the flagellin (FliC) of the fish pathogen Edwardsiel...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2010.06.022
更新日期:2010-08-02 00:00:00
abstract::Cellular as well as humoral immune reactivity were studied in healthy young (< 30 years; n = 12) and older (> 65 years; n = 12) individuals before as well as 1 month after immunization with a trivalent whole virus influenza vaccine. Before vaccination, peripheral blood mononuclear cell proliferation in response to in ...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/s0264-410x(97)88329-6
更新日期:1998-01-01 00:00:00
abstract::The carbohydrate moieties on HIV-1 envelope glycoprotein (Env) act as shields to mask conserved neutralizing epitopes, while the hyperimmunogenic variable regions are immunodominant in inducing non-neutralizing antibodies, representing the major challenge for using Env as a vaccine candidate to induce broadly neutrali...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2019.09.073
更新日期:2019-12-03 00:00:00
abstract::Numerous evidence demonstrate Type 1 diabetes (T1D) is due to a loss of immune tolerance to islet antigens, and CD8(+) T cells play an important role in the development of T1D in NOD mice. The novel coinhibitory receptor BTLA may have a regulatory role in maintaining peripheral tolerance, however, its role in autoimmu...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2011.07.125
更新日期:2011-10-13 00:00:00
abstract::In the present study the homologous and heterologous type and subtype specific cellular immune response induced by a wild type inactivated whole virus H5N1 Influenza (A/Vietnam/1203/2004) vaccine was evaluated. Two immunizations with the Vero cell derived H5N1 influenza vaccine on Day 0 and Day 21 induced significant ...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2010.10.065
更新日期:2010-12-16 00:00:00
abstract::We produced a Vero cell-derived inactivated Japanese encephalitis vaccine using a serum-free medium, as a substitute for the conventional mouse brain-derived Japanese encephalitis vaccine. The immunogenicity of this cell-derived vaccine was higher than that of the conventional mouse brain-derived vaccine. The results ...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2010.12.038
更新日期:2011-02-24 00:00:00
abstract::We conducted an active, population-based laboratory surveillance study to evaluate the epidemiologic features of invasive pneumococcal disease (IPD) in Taiwan. Concurrently, nasopharyngeal colonization of Streptococcus pneumoniae was evaluated among 1128 healthy children aged journal_title:Vaccine pub_type: 杂志文章,多中心研究 doi:10.1016/j.vaccine.2009.06.091 更新日期:2009-09-04 00:00:00
abstract::Since 2000, there has been a legal requirement in the UK that dogs and cats should have an effective rabies vaccination with demonstrable sero-conversion if their owners wish to avoid quarantine on re-entry to the UK. In 2002, 10,483 rabies titres were determined on dogs at the VLA. Statistical analyses assessed the e...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2007.10.015
更新日期:2007-12-12 00:00:00
abstract::Smallpox eradication, coordinated by the WHO and certified 40 years ago, led to the cessation of routine smallpox vaccination in most countries. It is estimated that over 70% of the world's population is no longer protected against smallpox, and through cross-immunity, to closely related orthopox viruses such as monke...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2020.04.062
更新日期:2020-07-14 00:00:00
abstract::Development of active targeted immunotherapeutics is a rapid developing field in the arena of chronic infectious diseases. The question of repeated, closely spaced administration of immunotherapeutics to achieve a rapid impact on the replicating agent is an important one. We analyzed here, using a prototype adenovirus...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2014.03.089
更新日期:2014-05-30 00:00:00
abstract::The amino terminal region of group A streptococcal M proteins evokes type-specific immunity while the conserved C-repeat epitopes evoke cross-protective immunity against multiple serotypes. The present studies were undertaken to compare the protective efficacy of vaccines containing either type-specific (hexavalent va...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2010.05.018
更新日期:2010-07-12 00:00:00
abstract:BACKGROUND AND OBJECTIVES:India has the highest under-five death toll globally, approximately 20% of which is attributed to vaccine-preventable diseases. India's Universal Immunization Programme (UIP) is working both to increase immunization coverage and to introduce new vaccines. Here, we analyze the disease and finan...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2014.04.080
更新日期:2014-08-11 00:00:00
abstract::Helicobacter pylori neutrophil-activating protein (NAP) is a toll-like receptor 2 (TLR2) agonist and potent immunomodulator inducing Th1-type immune response. Here we present data about characterization of the humoral immune response against NAP-tagged antigens, encoded by attenuated measles virus (MV) vector platform...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2013.07.085
更新日期:2013-10-01 00:00:00
abstract:PURPOSE:We conducted a cancer vaccine clinical trial with MAGE-A4 protein. Safety, clinical response, and antigen-specific immune responses were analyzed and the prognostic factors by vaccination were investigated. EXPERIMENTAL DESIGN:Twenty patients with advanced esophageal, stomach or lung cancer were administered M...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2014.09.002
更新日期:2014-10-14 00:00:00
abstract:OBJECTIVES:With the re-emergence of chikungunya virus (CHIKV) in an explosive form and in the absence of a commercially available vaccine, we aimed to develop candidate vaccines employing recombinant E2 protein or chemically inactivated whole virus. DESIGN AND METHODS:E2 gene of CHIKV isolate of ECSA genotype was clon...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2012.07.072
更新日期:2012-09-21 00:00:00
abstract:BACKGROUND:Few studies report the effectiveness of trivalent inactivated influenza vaccine (TIV) in preventing hospitalisation for influenza-confirmed respiratory infections. Using a prospective surveillance platform, this study reports the first such estimate from a well-defined ethnically diverse population in New Ze...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2014.04.013
更新日期:2014-06-17 00:00:00
abstract::The phrase "Process is the Product" is often applied to biologics, including multicomponent vaccines composed of complex components that evade complete characterization. Vaccine production processes must be defined and locked early in the development cycle to ensure consistent quality of the vaccine throughout scale-u...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2017.03.100
更新日期:2018-05-24 00:00:00
abstract::The Middle East respiratory syndrome coronavirus (MERS-CoV) is a highly pathogenic and zoonotic virus with a fatality rate in humans of over 35%. Although several vaccine candidates have been developed, there is still no clinically available vaccine for MERS-CoV. In this study, we developed two types of MERS-CoV vacci...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2018.04.082
更新日期:2018-06-07 00:00:00
abstract::Intranasal (i.n.) immunization is an effective regimen for the prophylaxis of respiratory diseases in early life. The aim of this study was to assess the need for nasal-associated lymphoid tissue (NALT) and cervical lymph nodes (CLN) in induction of protective immunity following mucosal vaccination of infant mice. We ...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2008.01.027
更新日期:2008-03-17 00:00:00
abstract::While immune checkpoint inhibition is rapidly becoming standard of care in many solid tumors, immune checkpoint inhibitors (ICIs) fail to induce clinical responses in many patients, presumably due to insufficient numbers of tumor-specific T cells in the tumor milieu. To this end, immunization protocols using viral vec...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2020.02.034
更新日期:2020-04-09 00:00:00
abstract::Pandemic outbreaks of influenza are caused by the emergence of a pathogenic and transmissible virus to which the human population is immunologically naïve. Recent outbreaks of highly pathogenic avian influenza (HPAI) of the H5N1 subtype are of particular concern because of the high mortality rate (60% case fatality ra...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2011.01.100
更新日期:2011-04-05 00:00:00
abstract::We employed DNA shuffling and screening technologies to develop a single recombinant dengue envelope (E) antigen capable of inducing neutralizing antibodies against all four antigenically distinct dengue serotypes. By DNA shuffling of codon-optimized dengue 1-4 E genes, we created a panel of novel chimeric clones expr...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2005.07.100
更新日期:2006-01-16 00:00:00
abstract::The neutralising antibody response after the yellow fever vaccine (YF-17D) skin test was measured in 7 egg allergic persons in whom further vaccination was abandoned because of a strong local urticarial reaction to the YF-17D vaccine test dose. We found that this test dose of 0.1mL of YF-17D vaccine was sufficient to ...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2009.02.049
更新日期:2009-04-21 00:00:00
abstract::Purified Vero cell culture rabies vaccine "Abhayrab" manufactured by Human Biologicals Institute, Ooty, India was subjected for immunogenicity studies. Pre-exposure study was undertaken on 60 healthy volunteers (Group I) with vaccination on days 0, 7 and 21. A group of 75 patients of category II (Group II), 67 of cate...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2004.08.001
更新日期:2005-01-04 00:00:00
abstract:INTRODUCTION:New vaccine introductions have put strains on vaccine supply chains around the world. While increasing storage and transportation may be the most straightforward options, it is also important to consider what financial and operational benefits can be incurred. In 2012, suboptimal vaccine coverage and impen...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2014.04.090
更新日期:2014-07-07 00:00:00
abstract:BACKGROUND:BCG vaccine, introduced almost 100years ago, is the only option to prevent TB disease. It effectively protects newborns from meningeal TB but fails to prevent adult pulmonary TB. TB kills 1.3million people annually in areas where BCG vaccination is widely practiced. Thus, more effective TB vaccines are urgen...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2017.02.065
更新日期:2017-04-11 00:00:00
abstract::Based on the reactivities of monoclonal antibodies, we previously reported that 25 Japanese encephalitis (JE) virus strains fell into five antigenic groups: Nakayama, Beijing-1, Kamiyama, 691004 and Muar. In the present study, we produced monoclonal antibodies against the Beijing-1 strain and obtained the Beijing-1 gr...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/0264-410x(94)90222-4
更新日期:1994-06-01 00:00:00
abstract:BACKGROUND:Launched in 1974, the Expanded Program on Immunization (EPI) is estimated to prevent two-three million deaths annually from polio, diphtheria, tuberculosis, pertussis, measles, and tetanus. Additional lives could be saved through better understanding what influences adherence to the EPI schedule in specific ...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2016.11.075
更新日期:2017-01-11 00:00:00
abstract:BACKGROUND:Elimination of congenital rubella syndrome depends not only on effective childhood immunization but also on the identification and immunization of rubella susceptible women. We assessed rubella susceptibility among pregnant women and evaluated the adherence and response to postpartum immunization with measle...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2015.02.043
更新日期:2015-04-08 00:00:00
abstract::The most common preventative measure against mumps is vaccination with mumps vaccine. In most parts of the world, mumps vaccine is routinely delivered through live attenuated Measles-Mumps-Rubella (MMR) vaccine. In Japan, receiving mumps vaccine is voluntary and vaccine uptake rate is less than 30%. The introduction o...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2014.05.020
更新日期:2014-07-16 00:00:00