Abstract:
PURPOSE:Published multigene classifiers suggesting outcome prediction for patients with stage UICC II colon cancer have not been translated into a clinical application so far. Therefore, we aimed at validating own and published gene expression signatures employing methods which enable their reconstruction in routine diagnostic specimens. METHODS:Immunohistochemistry was applied to 68 stage UICC II colon cancers to determine the protein expression of previously published prognostic classifier genes (CDH17, LAT, CA2, EMR3, and TNFRSF11A). RNA from macrodissected tumor samples from 53 of these 68 patients was profiled on Affymetrix GeneChips (HG-U133 Plus 2.0). Prognostic signatures were generated by "nearest shrunken centroids" with cross-validation. Previously published gene signatures were applied to our data set using "global tests" and leave-one-out cross-validation RESULTS:Correlation of protein expression with clinical outcome failed to separate patients with disease-free follow-up (group DF) and relapse (group R). Although gene expression profiling allowed the identification of differentially expressed genes ("DF" vs. "R"), a stable classification/prognosis signature was not discernable. Furthermore, the application of previously published gene signatures to our data was unable to predict clinical outcome (prediction rate 75.5% and 64.2%; n.s.). T-stage was the only independent prognostic factor for relapse with established clinical and pathological parameters including microsatellite status (multivariate analysis). CONCLUSIONS:Our protein and gene expression analyses do not support application of molecular classifiers for prediction of clinical outcome in current routine diagnostic as a basis for patient-orientated therapy in stage UICC II colon cancer. Further studies are needed to develop prognosis signatures applicable in patient care.
journal_name
Int J Colorectal Disjournal_title
International journal of colorectal diseaseauthors
Gröne J,Lenze D,Jurinovic V,Hummel M,Seidel H,Leder G,Beckmann G,Sommer A,Grützmann R,Pilarsky C,Mansmann U,Buhr HJ,Stein H,Hummel Mdoi
10.1007/s00384-011-1176-xsubject
Has Abstractpub_date
2011-07-01 00:00:00pages
847-58issue
7eissn
0179-1958issn
1432-1262journal_volume
26pub_type
杂志文章abstract::Colorectal surgery is still associated with a significant morbidity and mortality rate, mostly related to suture failure. We have carried out a randomized experimental study in dogs on colonic anastomoses exposed to a number of anastomotic risk situations. A total of 42 dogs was used. They were divided into three stud...
journal_title:International journal of colorectal disease
pub_type: 杂志文章
doi:10.1007/BF01647656
更新日期:1992-02-01 00:00:00
abstract::Division of the inferior mesenteric artery flush with the aorta (high tie) allows a tension-free anastomosis in distal colorectal resections but may also diminish the blood supply. Tissue oxygen tension was measured proximal to the resection margin before and after either low or high division of the inferior mesenteri...
journal_title:International journal of colorectal disease
pub_type: 杂志文章
doi:10.1007/BF00337583
更新日期:1995-01-01 00:00:00
abstract:BACKGROUND:The prognostic significance of anti-53 autoantibody in colorectal cancer (CRC) patients is unclear due to measurement of overall rather than disease-specific survival and generally short follow-up periods in many studies. We aim to investigate prognostic significance of anti-p53 auto-antibodies in a study wi...
journal_title:International journal of colorectal disease
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doi:10.1007/s00384-008-0458-4
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journal_title:International journal of colorectal disease
pub_type: 杂志文章,评审
doi:10.1007/s00384-003-0478-z
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abstract:BACKGROUND:Current guidelines support the use of adjuvant chemotherapy (CT) following neoadjuvant chemoradiotherapy (CTRT) and surgery to treat rectal cancer, although clinical trials have provided little evidence that it is effective. We performed a systematic review of published studies to assess whether adjuvant CT ...
journal_title:International journal of colorectal disease
pub_type: 杂志文章,meta分析,评审
doi:10.1007/s00384-014-2082-9
更新日期:2015-04-01 00:00:00
abstract:PURPOSE:Laparoscopy for colorectal cancer resection bares early post-operative advantages and results in equal oncologic long-term outcome. However, data on laparoscopic right hemi-colectomy is scarce. Aim of the present study was to analyze a well selected collective of patients with right-sided colon cancer treated o...
journal_title:International journal of colorectal disease
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doi:10.1007/s00384-016-2590-x
更新日期:2016-07-01 00:00:00
abstract:PURPOSE:Dendritic cell (DC)-based cancer vaccine represents a promising immunotherapy against cancer. There has been recent evidence which have suggested that toll-like receptor (TLR) ligands may be critical for DC preparation; this was usually omitted in the past. Our study is designed to investigate if the vaccinatio...
journal_title:International journal of colorectal disease
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doi:10.1007/s00384-012-1530-7
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journal_title:International journal of colorectal disease
pub_type: 杂志文章
doi:10.1007/s00384-012-1493-8
更新日期:2012-12-01 00:00:00
abstract:BACKGROUND:The impact of postoperative complications (POCs) on the long-term prognosis of patients with colorectal carcinoma was analysed with respect to their severity according to the Clavien-Dindo classification (CDC). METHODS:The prospectively collected data of 2158 patients who underwent curative resection of a c...
journal_title:International journal of colorectal disease
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abstract:INTRODUCTION:Emergency right hemicolectomy has been associated with dismal outcome. But, data in Asians is lacking as pathologies that require emergency right hemicolectomy may differ from that in the Western population. The aims of our study were to review our institution's experience with emergency right hemicolectom...
journal_title:International journal of colorectal disease
pub_type: 杂志文章
doi:10.1007/s00384-011-1203-y
更新日期:2011-09-01 00:00:00
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journal_title:International journal of colorectal disease
pub_type: 杂志文章
doi:10.1007/BF01660715
更新日期:1988-11-01 00:00:00
abstract:OBJECTIVE:Our study is the first meta-analysis to compare total mesorectal excision (TME) plus lateral lymph node dissection (LLND) with TME on rectal cancer patients regarding outcomes including overall survival, disease-free survival, local recurrence, complications, urinary dysfunction, and sexual dysfunction. METH...
journal_title:International journal of colorectal disease
pub_type: 杂志文章,评审
doi:10.1007/s00384-020-03610-1
更新日期:2020-06-01 00:00:00
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journal_title:International journal of colorectal disease
pub_type: 杂志文章
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更新日期:2018-05-01 00:00:00
abstract:PURPOSE:Appendectomy has been the gold standard for every form of appendicitis. In recent years, though, it has repeatedly been claimed that for acute uncomplicated appendicitis, antibiotic therapy can be an equivalent treatment. The aim of this meta-analysis was to determine if antibiotic therapy is a safe and effecti...
journal_title:International journal of colorectal disease
pub_type: 杂志文章,meta分析
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abstract:PURPOSE:Although small rectal carcinoid tumors can be treated using local excision, complete resection can be difficult because tumors are located in the submucosal layer. We evaluate the factors associated with pathologically complete local resection of rectal carcinoid tumors. METHODS:Data were analyzed of 161 patie...
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doi:10.1007/s00384-012-1538-z
更新日期:2013-01-01 00:00:00
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journal_title:International journal of colorectal disease
pub_type: 杂志文章
doi:10.1007/s00384-011-1342-1
更新日期:2012-04-01 00:00:00
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journal_title:International journal of colorectal disease
pub_type: 杂志文章
doi:10.1007/s003840100332
更新日期:2001-11-01 00:00:00
abstract:PURPOSE:It is controversial whether patients fare better with conservative or surgical treatment in certain stages of acute diverticulitis (AD), in particular when phlegmonous inflammation or covered micro- or macro-perforation are present. The aim of this study was to determine long-term quality of life (QoL) for AD p...
journal_title:International journal of colorectal disease
pub_type: 杂志文章
doi:10.1007/s00384-018-2969-y
更新日期:2018-03-01 00:00:00
abstract:BACKGROUND AND AIMS:This study used postoperative defecography to characterize morphological features of defecatory disorders in patients following rectal resection. We also evaluated differences in dynamic defecatory condition depending on reconstruction methods for sphincter-saving surgery. MATERIALS AND METHODS:Sub...
journal_title:International journal of colorectal disease
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abstract::Rectopexy in the sacral hollow or to the promontory with synthetic material is the most efficient method of reducing and fixing a complete rectal prolapse. However, this distressing condition occurs frequently in elderly patients, often with high operative risk. In these some surgeons have advocated a perineal approac...
journal_title:International journal of colorectal disease
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更新日期:1987-08-01 00:00:00
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journal_title:International journal of colorectal disease
pub_type: 杂志文章,meta分析,评审
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更新日期:2017-07-01 00:00:00
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journal_title:International journal of colorectal disease
pub_type: 杂志文章,多中心研究
doi:10.1007/s00384-007-0387-7
更新日期:2008-02-01 00:00:00
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journal_title:International journal of colorectal disease
pub_type: 杂志文章
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更新日期:2017-01-01 00:00:00
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journal_title:International journal of colorectal disease
pub_type: 杂志文章
doi:10.1007/s00384-002-0440-5
更新日期:2003-05-01 00:00:00
abstract::With the introduction of total mesorectal excision (TME) for treatment of rectal cancer, the prognosis of patients with rectal cancer is improved. With this better prognosis, there is a growing awareness about the quality of life of patients after rectal carcinoma. Laparoscopic total mesorectal excision (LTME) for rec...
journal_title:International journal of colorectal disease
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journal_title:International journal of colorectal disease
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更新日期:2008-07-01 00:00:00
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journal_title:International journal of colorectal disease
pub_type: 杂志文章,meta分析,评审
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更新日期:2018-10-01 00:00:00
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journal_title:International journal of colorectal disease
pub_type: 杂志文章
doi:10.1007/s00384-007-0303-1
更新日期:2007-10-01 00:00:00