Abstract:
:MicroRNAs (miRNAs) regulate cell physiology by altering protein expression, but the biology of platelet miRNAs is largely unexplored. We tested whether platelet miRNA levels were associated with platelet reactivity by genome-wide profiling using platelet RNA from 19 healthy subjects. We found that human platelets express 284 miRNAs. Unsupervised hierarchical clustering of miRNA profiles resulted in 2 groups of subjects that appeared to cluster by platelet aggregation phenotypes. Seventy-four miRNAs were differentially expressed (DE) between subjects grouped according to platelet aggregation to epinephrine, a subset of which predicted the platelet reactivity response. Using whole genome mRNA expression data on these same subjects, we computationally generated a high-priority list of miRNA-mRNA pairs in which the DE platelet miRNAs had binding sites in 3'-untranslated regions of DE mRNAs, and the levels were negatively correlated. Three miRNA-mRNA pairs (miR-200b:PRKAR2B, miR-495:KLHL5, and miR-107:CLOCK) were selected from this list, and all 3 miRNAs knocked down protein expression from the target mRNA. Reduced activation from platelets lacking PRKAR2B supported these findings. In summary, (1) platelet miRNAs are able to repress expression of platelet proteins, (2) miRNA profiles are associated with and may predict platelet reactivity, and (3) bioinformatic approaches can successfully identify functional miRNAs in platelets.
journal_name
Bloodjournal_title
Bloodauthors
Nagalla S,Shaw C,Kong X,Kondkar AA,Edelstein LC,Ma L,Chen J,McKnight GS,López JA,Yang L,Jin Y,Bray MS,Leal SM,Dong JF,Bray PFdoi
10.1182/blood-2010-09-299719subject
Has Abstractpub_date
2011-05-12 00:00:00pages
5189-97issue
19eissn
0006-4971issn
1528-0020pii
blood-2010-09-299719journal_volume
117pub_type
杂志文章相关文献
BLOOD文献大全abstract::Using urea-solubilized human fibrin monomer as an immunogen, we raised in mice a battery of monoclonal antibodies that reacted with the immunogen but not with urea-treated or native fibrinogen. Although they all failed to react with acid-solubilized fibrin monomer (acid-FM) alone, an antibody designated as IF-43 was f...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1996-09-15 00:00:00
abstract::The malignant Reed-Sternberg cell of Hodgkin disease is an aberrant B cell that persists in an immunolgically mediated inflammatory infiltrate. Despite its nonproductive immunoglobulin genes, the Reed-Sternberg cell avoids the usual apoptotic fate of defective immune cells through an unknown mechanism. A likely candid...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:2000-10-15 00:00:00
abstract::High-molecular weight DNAs of fresh bone marrow cells from 32 patients with fresh leukemia were assayed for the presence of transmissible activated transforming genes by a DNA-mediated gene transfer technique using NIH/3T3 cells. DNAs of bone marrow cells from four of the 32 patients induced transformation of NIH/3T3 ...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1985-12-01 00:00:00
abstract::The effect of recombinant human erythropoietin (rHuEpo) on megakaryopoiesis remains controversial. Treatment with rHuEpo in renal failure patients has been associated with a slight elevation of platelet counts. In animal studies, high doses of rHuEpo produced an increase of platelet counts followed by a gradual return...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1999-05-15 00:00:00
abstract::Congenital thrombocytopenia may occur in isolation or accompanied by eczema and immunodeficiency, as part of the X-linked hereditary Wiskott-Aldrich syndrome (WAS). Because the clinical and immunologic picture of WAS is variable, particularly early in life, definite diagnosis cannot always be made in cases with a nega...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1990-06-15 00:00:00
abstract::Hereditary glutathione reductase (GR) deficiency was found in only 2 cases when testing more than 15 000 blood samples. We have investigated the blood cells of 2 patients (1a and 1b) in a previously described family suffering from favism and cataract and of a novel patient (2) presenting with severe neonatal jaundice....
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2006-08-042531
更新日期:2007-04-15 00:00:00
abstract::Obinutuzumab is a type 2, glycoengineered, anti-CD20 antibody recently approved with chlorambucil for the initial therapy of chronic lymphocytic leukemia (CLL). In this nonrandomized, parallel-cohort, phase 1b, multicenter study, we explored the safety and preliminary efficacy of obinutuzumab-bendamustine (G-B) or obi...
journal_title:Blood
pub_type: 杂志文章,多中心研究
doi:10.1182/blood-2014-12-613570
更新日期:2015-04-30 00:00:00
abstract::Growing evidence supports a pathophysiological role for platelets during the manifestation of postischemic reperfusion injury; in the current study, we investigated the nature and the molecular determinants of platelet-endothelial cell interactions induced by ischemia/reperfusion (I/R). Platelet-endothelium and leukoc...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1998-07-15 00:00:00
abstract::Myeloid-derived suppressor cells (MDSCs) represent a heterogeneous population that shares certain characteristics including an aberrant myeloid phenotype and the ability to suppress T cells. MDSCs have been predominantly studied in malignant diseases and findings suggest involvement in tumor-associated immune suppress...
journal_title:Blood
pub_type: 临床试验,杂志文章
doi:10.1182/blood-2013-12-546416
更新日期:2014-07-31 00:00:00
abstract::Umbilical cord blood (UCB) is an attractive cell source for hematopoietic cell transplantation (HCT). Here we examine whether the combination of homeobox B4 (HOXB4) and Delta-1 ligand (DL) synergize when used together. Monkey and human UCB CD34(+) cells were transduced with a HOXB4-expressing gammaretroviral vector an...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2010-05-286062
更新日期:2010-12-23 00:00:00
abstract::Adhesive interactions between circulating sickle red blood cells (RBCs), leukocytes, and endothelial cells are major pathophysiologic events in sickle cell disease (SCD). To develop new therapeutics that efficiently inhibit adhesive interactions, we generated an anti-P-selectin aptamer and examined its effects on cell...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2010-05-285718
更新日期:2011-01-13 00:00:00
abstract::An association between platelets, angiogenesis, and cancer has long been recognized, but the mechanisms linking them remains unclear. Platelets regulate new blood vessel growth through numerous stimulators and inhibitors of angiogenesis by several pathways, including differential exocytosis of angiogenesis regulators....
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2011-02-334524
更新日期:2011-08-04 00:00:00
abstract::The biochemical basis for complement acting directly on antigen-presenting cells to enhance their function in T-cell stimulation has been unclear. Here we present evidence that engagement of C3a receptor (C3aR) on the surface of dendritic cells (DCs) leads to alterations in the level of intracellular cyclic adenosine ...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2008-05-156646
更新日期:2008-12-15 00:00:00
abstract::It is estimated that in excess of 300,000 children are born each year with a severe inherited disorder of hemoglobin and that approximately 80% of these births occur in low- or middle-income countries. As these countries go through an epidemiologic transition, with a reduction in childhood and infant mortality due to ...
journal_title:Blood
pub_type: 杂志文章,评审
doi:10.1182/blood-2010-01-251348
更新日期:2010-06-03 00:00:00
abstract::Immunoaffinity chromatography using conformation-specific antibodies yields pure factor IX from human plasma in a single rapid, facile purification step. We evaluated this technique to determine whether factor IX can be separated from human T cell leukemia virus-I (HTLV-I) and human immunodeficiency virus (HIV) in pla...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1987-11-01 00:00:00
abstract::Hepcidin is the master regulator of iron homeostasis. In the liver, iron-dependent hepcidin activation is regulated through Bmp6 and its membrane receptor hemojuvelin (Hjv), whereas, in response to iron deficiency, hepcidin repression seems to be controlled by a pathway involving the serine protease matriptase-2 (enco...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2010-07-295147
更新日期:2011-01-13 00:00:00
abstract::Tumor-associated macrophages (TAM) are important components of the multiple myeloma (MM) microenvironment that support malignant plasma cell survival and resistance to therapy. It has been proposed that macrophages (MØ) retain the capacity to change in response to stimuli that can restore their antitumor functions. He...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2016-01-695395
更新日期:2016-11-03 00:00:00
abstract::Chronic graft-v-host disease (chronic GVHD) is a frequent cause of late morbidity and death after bone marrow transplantation (BMT). The actuarial survival after onset of chronic GVHD in 85 patients was 42% (95%Cl = 29%, 54%) at 10 years. Baseline characteristics present at the onset of chronic GVHD (before therapy) i...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1989-09-01 00:00:00
abstract::Approximately 4% to 5% of all bone marrow (BM) cells and 8% to 9% of low density BM cells from FVB/N and BALB/c mice (Ly-6a haplotype) show high to intermediate expression of Ly-6E.1 antigen, recognized by the Sca-1 antibody. Functional properties of enriched cells expressing Ly-6E.1-allelic form of Sca-1 antigen were...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1993-11-01 00:00:00
abstract::Angiogenesis, the formation of new capillaries from preexisting blood vessels, is a multistep, highly orchestrated process involving vessel sprouting, endothelial cell migration, proliferation, tube differentiation, and survival. Eicosanoids, arachidonic acid (AA)-derived metabolites, have potent biologic activities o...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:2000-04-01 00:00:00
abstract::Omenn syndrome (OS) is an inherited disorder characterized by an absence of circulating B cells and an infiltration of the skin and the intestine by activated oligoclonal T lymphocytes, indicating that a profound defect in the lymphoid developmental program could be accountable for this condition. Inherited mutations ...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood.v97.9.2772
更新日期:2001-05-01 00:00:00
abstract::Multiple myeloma (MM) is characterized by accumulation and dissemination of malignant plasma cells (PCs) in the bone marrow (BM). Gene expression profiling of 2 MM cell lines (OH-2 and IH-1) indicated that expression of PRL-3, a metastasis-associated tyrosine phosphatase, was induced by several mitogenic cytokines. Cy...
journal_title:Blood
pub_type: 杂志文章,多中心研究
doi:10.1182/blood-2007-07-101139
更新日期:2008-01-15 00:00:00
abstract::Hematopoietic stem cells (HSCs) undergo dramatic expansion during fetal liver development, but attempts to expand their numbers ex vivo have failed. We hypothesized that unidentified fetal liver cells produce growth factors that support HSC proliferation. Here we describe a novel population of CD3+ and Ter119- day-15 ...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2003-08-2955
更新日期:2004-04-01 00:00:00
abstract::Artemisinin resistance threatens worldwide malaria control and elimination. Elevation of phosphatidylinositol-3-phosphate (PI3P) can induce resistance in blood stages of Plasmodium falciparum The parasite unfolded protein response (UPR) has also been implicated as a proteostatic mechanism that may diminish artemisinin...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2017-11-814665
更新日期:2018-03-15 00:00:00
abstract::In this report, we describe a modification of the assay for long-term culture-initiating cells (LTC-IC) that allows a subset of murine LTC-IC (designated as LTC-ICML) to express both their myeloid (M) and lymphoid (L) differentiative potentials in vitro. The modified assay involves culturing test cells at limiting dil...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1995-08-15 00:00:00
abstract::Recent studies have shown that soluble factors elaborated by human T lymphocytes enhance erythroid burst formation by human peripheral blood null cells. This study demonstrates that media conditioned by a long-term T lymphocyte line augmented the growth of erythroid colonies in vitro in the presence of erythropoietin ...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1980-10-01 00:00:00
abstract::Activation of inflammatory and procoagulant mechanisms is thought to contribute significantly to the initiation of restenosis, a common complication after balloon angioplasty of obstructed arteries. During this process, expression of tissue factor (TF) represents one of the major physiologic triggers of coagulation th...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2003-04-1148
更新日期:2004-03-01 00:00:00
abstract::Oncogenic EZH2 is overexpressed and extensively involved in the pathophysiology of different cancers including extranodal natural killer/T-cell lymphoma (NKTL). However, the mechanisms regarding EZH2 upregulation is poorly understood, and it still remains untargetable in NKTL. In this study, we examine EZH2 protein tu...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood.2019000381
更新日期:2019-12-05 00:00:00
abstract::Von Willebrand factor (VWF) is a key hemostatic protein synthesized in both endothelial cells and megakaryocytes. Megakaryocyte-derived VWF is stored in α-granules of platelets and is enriched in hyperactive "ultra-large" VWF multimers. To elucidate the specific contribution of platelet VWF in hemostasis and thrombosi...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2015-03-632901
更新日期:2015-10-01 00:00:00
abstract::Graft-versus-host disease (GVHD) remains one of the major complications after allogeneic bone marrow transplantation (allo-BMT). Sirtuin-1 (Sirt-1) plays a crucial role in various biological processes including cellular senescence, metabolism, and inflammatory responses. Sirt-1 deacetylation regulates different transc...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2018-07-863233
更新日期:2019-01-17 00:00:00