Abstract:
:Immunoaffinity chromatography using conformation-specific antibodies yields pure factor IX from human plasma in a single rapid, facile purification step. We evaluated this technique to determine whether factor IX can be separated from human T cell leukemia virus-I (HTLV-I) and human immunodeficiency virus (HIV) in plasma supplemented with these viruses. Viral content was determined with an enzyme-linked immunosorbent (ELISA) assay sensitive to 50 ng viral protein. Both HTLV-I and HIV coeluted with unbound protein. Neither HTLV-I nor HIV was detected in purified factor IX. We conclude that, to the limits of detection, factor IX purified by this method is free of viral contamination.
journal_name
Bloodjournal_title
Bloodauthors
Limentani SA,Furie BC,Poiesz BJ,Montagna R,Wells K,Furie Bsubject
Has Abstractpub_date
1987-11-01 00:00:00pages
1312-5issue
5eissn
0006-4971issn
1528-0020journal_volume
70pub_type
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