Involvement of the heme oxygenase-carbon monoxide-cGMP pathway in the nociception induced by acute painful stimulus in rats.


:Heme oxygenase-carbon monoxide-cGMP (HO-CO-cGMP) pathway has been reported to be involved in peripheral and spinal modulation of inflammatory pain. However, the involvement of this pathway in the modulation of acute painful stimulus in the absence of inflammation remains unknown. Thus, we evaluated the involvement of the HO-CO-cGMP pathway in nociception by means the of analgesia index (AI) in the tail flick test. Rats underwent surgery for implantation of unilateral guide cannula directed toward the lateral ventricle and after the recovery period (5-7 days) were subjected to the measures of baseline tail flick test. Animals were divided into groups to assess the effect of intracerebroventricular administration (i.c.v.) of the following compounds: ZnDPBG (HO inhibitor) or vehicle (Na(2)CO(3)), heme-lysinate (substrate overload) or vehicle (l-lysine), or the selective inhibitor of soluble guanilate cyclase ODQ or vehicle (DMSO 1%) following the administration of heme-lysinate or vehicle. Heme overload increased AI, indicating an antinociceptive role of the pathway. This response was attenuated by i.c.v. pretreatment with the HO inhibitor ZnDPBG. In addition, this effect was dependent on cGMP activity, since the pretreatment with ODQ blocked the increase in the AI. Because CO produces most of its actions via cGMP, these data strongly imply that CO is the HO product involved in the antinociceptive response. This modulation seems to be phasic rather than tonic, since i.c.v. treatment with ZnDPBG or ODQ did not alter the AI. Therefore, we provide evidence consistent with the notion that HO-CO-cGMP pathway plays a key phasic antinociceptive role modulating noninflammatory acute pain.


Brain Res


Brain research


Carvalho PG,Branco LG,Panissi CR




Has Abstract


2011-04-18 00:00:00












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    journal_title:Brain research

    pub_type: 杂志文章


    authors: Altobelli GG,Cimini V

    更新日期:2007-02-09 00:00:00

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    abstract::Perinatal exposure to environmental tobacco smoke (ETS) has adverse effects on neurobehavioral development. In the current study, rats were exposed to ETS during gestation, during the early neonatal period, or both. Brains and hearts were examined for alterations in adenylyl cyclase (AC) activity and for changes in be...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Slotkin TA,Pinkerton KE,Garofolo MC,Auman JT,McCook EC,Seidler FJ

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  • Role of potassium channels in the central neurogenic neuroprotection elicited by cerebellar stimulation in rat.

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    journal_title:Brain research

    pub_type: 杂志文章


    authors: Golanov EV,Christensen JD,Reis DJ

    更新日期:1999-09-25 00:00:00

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    abstract::Isolated brain capillary endothelial cells contain high activity levels of the blood-brain barrier (BBB) marker enzymes gamma-glutamyltranspeptidase (gamma-GT) and alkaline phosphatase (ALP). In primary culture the activities of these specific enzymes decrease with increasing cell proliferation to a constant low value...

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    pub_type: 杂志文章


    authors: Meyer J,Mischeck U,Veyhl M,Henzel K,Galla HJ

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  • Characterization of [3H]cytisine binding to human brain membrane preparations.

    abstract::The binding characteristics of [3H]cytisine, a putative CNS nicotinic receptor ligand, were examined in 4 regions of the human brain. [3H]Cytisine was found to bind non-cooperatively with high affinity to a single site in tissue homogenates and to exhibit low non-specific association. The binding characteristics of th...

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    pub_type: 杂志文章


    authors: Hall M,Zerbe L,Leonard S,Freedman R

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  • Neurotensin increases the cationic conductance of rat substantia nigra dopaminergic neurons through the inositol 1,4,5-trisphosphate-calcium pathway.

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    journal_title:Brain research

    pub_type: 杂志文章


    authors: Wu T,Li A,Wang HL

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    authors: Sagen J,Proudfit HK

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    authors: Schultzberg M,Dalsgaard CJ

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    journal_title:Brain research

    pub_type: 杂志文章


    authors: Wigström H,Swann JW

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    更新日期:2000-03-06 00:00:00

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    authors: Dryer SE,Chiappinelli VA

    更新日期:1985-09-01 00:00:00

  • Age-related alterations in GABA(A) receptor subunits in the nonhuman primate hippocampus.

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    pub_type: 杂志文章


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    authors: De Schutter E

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  • Theophylline-induced respiratory recovery following cervical spinal cord hemisection is augmented by serotonin 2 receptor stimulation.

    abstract::Cervical spinal cord hemisection leads to a disruption of bulbospinal innervation of phrenic motoneurons resulting in paralysis of the ipsilateral hemidiaphragm. We have previously demonstrated separate therapeutic roles for theophylline, and more recently serotonin (5-HT) as modulators to phrenic nerve motor recovery...

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    authors: Basura GJ,Nantwi KD,Goshgarian HG

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    authors: Maehara S,Hikichi H,Ohta H

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  • Calcium/calmodulin-dependent protein kinase II immunostaining is preserved in Alzheimer's disease hippocampal neurons.

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    journal_title:Brain research

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    authors: Simonian NA,Elvhage T,Czernik AJ,Greengard P,Hyman BT

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    authors: Fitts DA,Thunhorst RL,Simpson JB

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  • Involvement of dopamine D1 receptors of the central amygdala on the acquisition and expression of morphine-induced place preference in rat.

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    authors: Zarrindast MR,Rezayof A,Sahraei H,Haeri-Rohani A,Rassouli Y

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    journal_title:Brain research

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    authors: Reyher CK

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    authors: Torigoe K,Awaya A

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    authors: Furlong T,Carrive P

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    journal_title:Brain research

    pub_type: 杂志文章


    authors: Tashiro N,Gallagher JP,Nishi S

    更新日期:1976-12-10 00:00:00

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    journal_title:Brain research

    pub_type: 杂志文章


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    pub_type: 杂志文章


    authors: Mason GF,Martin DL,Martin SB,Manor D,Sibson NR,Patel A,Rothman DL,Behar KL

    更新日期:2001-09-28 00:00:00