Regulation of ERK1/2 activity upon contact inhibition in fibroblasts.


:Contact inhibition is a crucial mechanism regulating proliferation in vitro and in vivo. Despite its generally accepted importance for maintaining tissue homeostasis knowledge about the underlying molecular mechanisms of contact inhibition is still scarce. Since the MAPK ERK1/2 plays a pivotal role in the control of proliferation, we investigated regulation of ERK1/2 phosphorylation which is downregulated in confluent NIH3T3 cultures. We found a decrease in upstream signaling including phosphorylation of the growth factor receptor adaptor protein ShcA and the MAPK kinase MEK1/2 in confluent compared to exponentially growing cultures whereas involvement of ERK1/2 phosphatases in ERK1/2 inactivation is unlikely. Treatment of confluent, serum-deprived cultures with PDGF-B resulted in similar phosphorylation of ERK1/2 and induction of DNA-synthesis as detected in sparse, serum-deprived cultures. In contrast, ERK1/2 phosphorylation and DNA-synthesis could not be stimulated in confluent, serum-deprived cultures exposed to EGF. Our data indicate that PDGFR- and EGFR signaling are differentially inhibited in confluent cultures of NIH3T3 cells.


Küppers M,Faust D,Linz B,Dietrich C




Has Abstract


2011-03-18 00:00:00














  • Noninvasive, targeted gene therapy for acute spinal cord injury using LIFU-mediated BDNF-loaded cationic nanobubble destruction.

    abstract::Various gene delivery systems have been widely studied for the acute spinal cord injury (SCI) treatment. In the present study, a novel type of brain-derived neurotrophic factor (BDNF)-loaded cationic nanobubbles (CNBs) conjugated with MAP-2 antibody (mAbMAP-2/BDNF/CNBs) was prepared to provide low-intensity focused ul...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Song Z,Ye Y,Zhang Z,Shen J,Hu Z,Wang Z,Zheng J

    更新日期:2018-02-12 00:00:00

  • Annexin II is a novel receptor for Pseudomonas aeruginosa.

    abstract::Infections with Pseudomonas aeruginosa (P. aeruginosa) are critical in ventilated and poly-traumatized patients. Most important, these bacteria cause frequent and chronic pulmonary infections in patients with cystic fibrosis. Therefore, identification of molecular mechanisms that mediate the infection of mammalian cel...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Kirschnek S,Adams C,Gulbins E

    更新日期:2005-02-18 00:00:00

  • DSCAM: an endogenous promoter drives expression in the developing CNS and neural crest.

    abstract::The development of central nervous system (CNS) neuronal networks involves processes including neuroblast migration, axonal pathfinding, and synaptogenesis. To evaluate the role of the axonal guidance molecule DSCAM in CNS connectivity, we generated a lacZ reporter construct, Pr1.8-betagal, containing a 1.8kb fragment...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Barlow GM,Lyons GE,Richardson JA,Sarnat HB,Korenberg JR

    更新日期:2002-11-22 00:00:00

  • Alpha/beta hydrolase 1 is upregulated in D5 dopamine receptor knockout mice and reduces O2- production of NADPH oxidase.

    abstract::Renal dopamine receptors have been shown to play a critical role in ROS-dependent hypertension. D5 dopamine receptor deficient (D5-/-) mice are hypertensive and have increased systemic oxidative stress which is manifested in the kidney and the brain. To further investigate the underlying mechanisms of hypertension in ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Stoelting M,Geyer M,Reuter S,Reichelt R,Bek MJ,Pavenstädt H

    更新日期:2009-01-30 00:00:00

  • STI-571: an anticancer protein-tyrosine kinase inhibitor.

    abstract::STI-571 (imatinib, Gleevec, Glivec, CGP 57148) is an inhibitor of the Abl group of protein-tyrosine kinases. One of these enzymes, the Bcr-Abl oncoprotein, results from the fusion of the BCR and ABL genes that result from the reciprocal chromosomal translocation that forms the Philadelphia chromosome. The Philadelphia...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章,评审


    authors: Roskoski R Jr

    更新日期:2003-10-03 00:00:00

  • Induction of pluripotency in mammalian fibroblasts by cell fusion with mouse embryonic stem cells.

    abstract:BACKGROUND:Cell fusion is a phenomenon that is observed in various tissues in vivo, resulting in acquisition of physiological functions such as liver regeneration. Fused cells such as hybridomas have also been produced artificially in vitro. Furthermore, it has been reported that cellular reprogramming can be induced b...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Imai H,Kusakabe KT,Kiso Y,Hattori S,Kai C,Ono E,Kano K

    更新日期:2020-01-01 00:00:00

  • Affinity purification of HIV-1 and HIV-2 proteases from recombinant E. coli strains using pepstatin-agarose.

    abstract::A procedure is described which employs pepstatin-agarose for the affinity purification of either HIV-1 or HIV-2 protease from two similar recombinant E. coli constructs that were developed for the expression of these enzymes. HIV-2 protease was routinely expressed at much higher levels than the HIV-1 enzyme and pepsta...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Rittenhouse J,Turon MC,Helfrich RJ,Albrecht KS,Weigl D,Simmer RL,Mordini F,Erickson J,Kohlbrenner WE

    更新日期:1990-08-31 00:00:00

  • The main protease and RNA-dependent RNA polymerase are two prime targets for SARS-CoV-2.

    abstract::The coronavirus disease 2019 (COVID-19) pandemic, caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), poses an unprecedented global health crisis. It is particularly urgent to develop clinically effective therapies to contain the pandemic. The main protease (Mpro) and the RNA-dependent RNA poly...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Jin Z,Wang H,Duan Y,Yang H

    更新日期:2020-11-21 00:00:00

  • APP gene promoter constructs are preferentially expressed in the CNS and testis of transgenic mice.

    abstract::Transgenic animals were used to examine the spatial and temporal regulation of the human beta amyloid precursor protein (APP) gene promoter region in vivo. A 2.9 kb DNA fragment encompassing the APP gene promoter was fused to the chloramphenical acetyltransferase (CAT) reporter gene (pAMY-CAT) or a partial cDNA encodi...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Fox NW,Johnstone EM,Ward KE,Schrementi J,Little SP

    更新日期:1997-11-26 00:00:00

  • Why is phosphonodifluoromethyl phenylalanine a more potent inhibitory moiety than phosphonomethyl phenylalanine toward protein-tyrosine phosphatases?

    abstract::The phosphonodifluoromethyl phenylalanine (F2Pmp) is superior to phosphonomethyl phenylalanine (Pmp) as a non-hydrolyzable phosphotyrosine (pTyr) mimetic. The difluoromethyl moiety increases the inhibitory potency of a F2Pmp-containing peptide over a Pmp-containing counterpart by 1000-fold toward the protein tyrosine ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Chen L,Wu L,Otaka A,Smyth MS,Roller PP,Burke TR Jr,den Hertog J,Zhang ZY

    更新日期:1995-11-22 00:00:00

  • Protein phosphatase 1 of Leishmania donovani exhibits conserved catalytic residues and pro-inflammatory response.

    abstract::Protein phosphorylation, governed by kinases and phosphatases, plays a pivotal role in enormous cellular signaling pathways. Although PPP family of serine/threonine phosphatases have been involved in multiplication and growth of trypanosomatid parasites, but comprehensive knowledge is still very limited. In the presen...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Qureshi R,Jakkula P,Sagurthi SR,Qureshi IA

    更新日期:2019-08-27 00:00:00

  • miR-137 inhibits glutamine catabolism and growth of malignant melanoma by targeting glutaminase.

    abstract::Glutamine catabolism is considered to be an important metabolic pathway for cancer cells. Glutaminase (GLS) is the important rate-limiting enzyme of glutamine catabolism. miR-137 functions as a tumor suppressor in many human malignant tumors. However, the role and molecular mechanism of miR-137 and GLS in malignant me...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Luan W,Zhou Z,Zhu Y,Xia Y,Wang J,Xu B

    更新日期:2018-01-01 00:00:00

  • Gas6/Axl mediates tumor cell apoptosis, migration and invasion and predicts the clinical outcome of osteosarcoma patients.

    abstract::Dysregulation of the receptor tyrosine kinase Axl and its ligand Gas6 has been shown to promote multiple tumorigenic processes, as well as to correlate with worse prognosis in many different tumor types. However, studies of Axl expression and function in osteosarcoma have rarely been reported. In this study, we report...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Han J,Tian R,Yong B,Luo C,Tan P,Shen J,Peng T

    更新日期:2013-06-07 00:00:00

  • Hsa_circ_0006872 promotes cigarette smoke-induced apoptosis, inflammation and oxidative stress in HPMECs and BEAS-2B cells through the miR-145-5p/NF-κB axis.

    abstract::Cigarette smoke is a major cause of chronic obstructive pulmonary disease (COPD). Circular RNAs (circRNAs) are involved in regulating various biological processes. This study aimed to explore the role and molecular basis of hsa_circ_0006872 in cigarette smoke extract (CSE)-induced cell injury. HPMECs and BEAS-2B cells...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Xue M,Peng N,Zhu X,Zhang H

    更新日期:2021-01-01 00:00:00

  • Oleuropein, a non-toxic olive iridoid, is an anti-tumor agent and cytoskeleton disruptor.

    abstract::Oleuropein, a non-toxic secoiridoid derived from the olive tree, is a powerful antioxidant and anti-angiogenic agent. Here, we show it to be a potent anti-cancer compound, directly disrupting actin filaments in cells and in a cell-free assay. Oleuropein inhibited the proliferation and migration of advanced-grade tumor...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Hamdi HK,Castellon R

    更新日期:2005-09-02 00:00:00

  • Lipid fluidity at different regions in LDL and HDL of beta-thalassemia/Hb E patients.

    abstract::Atherosclerosis-related vascular complications in beta-thalassemia/hemoglobin E (beta-thal/Hb E) patients may result from iron induced oxidation of lipoproteins. To identify the specific site of oxidative damage, changes in lipid fluidity at different regions in LDL and HDL particle were investigated using two fluores...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Morales NP,Charlermchoung C,Luechapudiporn R,Yamanont P,Fucharoen S,Chantharaksri U

    更新日期:2006-11-24 00:00:00

  • Mouse thymoma cell line expresses a gluconeogenic enzyme, fructose 1,6-bisphosphatase.

    abstract::Fructose 1,6-bisphosphatase was observed in a thymic lymphoma cell line, WEH17.1 (11.5 +/- 0.8 munits/mg cytosol protein). Only a trace amount of the enzyme activity was observed in normal thymus tissue. The WEH17.1 enzyme had a pH optimum at around 7.5. The AMP-concentration giving 50% inhibition of the activity was ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Mizunuma H,Tashima Y

    更新日期:1989-02-15 00:00:00

  • Lysis of trypanosomes by peptidyl fluoromethyl ketones.

    abstract::Peptidyl fluoromethyl ketones, improved reagents for inactivating cysteinyl and serine proteinases, have provided unexpected results when applied to intact trypanosomes. A lethal effect was observed but limited to the infectious phase of the parasitic growth cycle. Since the inhibitors are known only to act on proteas...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Ashall F,Angliker H,Shaw E

    更新日期:1990-07-31 00:00:00

  • Drosophila melanogaster larval hemolymph protein mapping.

    abstract::With the completion of the genome sequence of Drosophila melanogaster the importance of constructing a proteome map is to be considered. Therefore, with the application of recent advances in proteomic analysis approaches, a protein map of D. melanogaster larvae hemolymph proteins was obtained using 2-DE in the range o...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Guedes Sde M,Vitorino R,Tomer K,Domingues MR,Correia AJ,Amado F,Domingues P

    更新日期:2003-12-19 00:00:00

  • Oxidative DNA damage by minor metabolites of toluene may lead to carcinogenesis and reproductive dysfunction.

    abstract::Recently, the concern that toluene might have carcinogenic and reproductive toxic potential has been raised. We investigated the ability of DNA damage by minor metabolites of toluene, methylhydroquinone, and methylbenzoquinone, using (32)P-5'-end-labeled DNA fragments obtained from the human genes. Methylhydroquinone ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Murata M,Tsujikawa M,Kawanishi S

    更新日期:1999-08-02 00:00:00

  • Knockout of immunoproteasome subunit β2i ameliorates cardiac fibrosis and inflammation in DOCA/Salt hypertensive mice.

    abstract::The immunoproteasome is a multicatalytic protease complex in all eukaryotic cells, which plays a key role in regulating essential cellular processes. However, the role of immunoproteasome subunit β2i in regulation of cardiac fibrosis and inflammation in deoxycorticosterone-acetate (DOCA)/salt mice remains unknown. Wil...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Yan W,Bi HL,Liu LX,Li NN,Liu Y,Du J,Wang HX,Li HH

    更新日期:2017-08-19 00:00:00

  • Activation of the Wnt/β-catenin pathway in pancreatic beta cells during the compensatory islet hyperplasia in prediabetic mice.

    abstract::The Wnt/β-catenin signaling pathway, also known as the canonical Wnt pathway, plays a role in cell proliferation and differentiation in several tissues/organs. It has been recently described in humans a relationship between type 2 diabetes (T2DM) and mutation in the gene encoding the transcription factor TCF7L2 associ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Maschio DA,Oliveira RB,Santos MR,Carvalho CP,Barbosa-Sampaio HC,Collares-Buzato CB

    更新日期:2016-09-30 00:00:00

  • Role of N-myristoylation in stability and subcellular localization of the CLPABP protein.

    abstract::Cardiolipin and phosphatidic acid-binding protein (CLPABP) controls the stability of the mRNA harboring an AU-rich element (ARE) in the 3' UTR with the help of the RNA stabilizer, human antigen R (HuR). Although CLPABP is localized on the mitochondrial surface as a large protein-RNA complex, its precise role is not ye...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Maeda A,Uchida M,Nishikawa S,Nishino T,Konishi H

    更新日期:2018-01-01 00:00:00

  • CXCR4 and PTEN are involved in the anti-metastatic regulation of anethole in DU145 prostate cancer cells.

    abstract::Anethole has been known to have chemopreventive activities as a suppressor of the incidence and multiplicity of both invasive and noninvasive carcinomas. The goal of this study was to understand the anti-metastatic effect of anethole through C-X-C chemokine receptor type 4 (CXCR4)/tumor suppressor phosphatase and tens...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Rhee YH,Chung PS,Kim SH,Ahn JC

    更新日期:2014-05-16 00:00:00

  • Heteronuclear 113Cd-1H NMR study of metal coordination in the human retinoic acid receptor-beta DNA binding domain.

    abstract::The two zinc fingers of the DNA binding domain of the human retinoic acid receptor-beta were labelled with 113Cd. Two- and three-dimensional heteronuclear nuclear magnetic resonance (NMR) experiments show that the first eight conserved cysteine residues coordinate the two zinc ions tetrahedrally. The ninth conserved c...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Knegtel RM,Boelens R,Ganadu ML,George AV,van der Saag PT,Kaptein R

    更新日期:1993-04-30 00:00:00

  • Overexpression of hepatitis delta antigen protects insect cells from baculovirus-induced cytolysis.

    abstract::Hepatitis delta virus (HDV) is a human pathogen causing fulminant hepatitis and liver cirrhosis. HDV has a circular single-stranded RNA genome, which encodes only a single protein, hepatitis delta antigen (HDAg), from the antigenomic strand. Although the functional roles of HDAg have been extensively studied, the mole...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Hwang SB,Park KJ,Kim YS

    更新日期:1998-03-27 00:00:00

  • Proteomic analysis of spinal cord of presymptomatic amyotrophic lateral sclerosis G93A SOD1 mouse.

    abstract::Amyotrophic lateral sclerosis (ALS) is a fatal motor neuron disease, whose primary mechanisms or causes are still not defined and for which no effective treatment is available. We have recently reported that before disease onset the level of tyrosine nitrated proteins is increased in the G93A SOD1 transgenic mouse mod...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Massignan T,Casoni F,Basso M,Stefanazzi P,Biasini E,Tortarolo M,Salmona M,Gianazza E,Bendotti C,Bonetto V

    更新日期:2007-02-16 00:00:00

  • Vascular smooth muscle actions and receptor interactions of 8-iso-prostaglandin E2, an E2-isoprostane.

    abstract::8-iso-PGE2, an E2-isoprostane, decreased GFR and RPF dose-dependently in rats, but with lesser potency than 8-epi-PGF2 alpha, an F2-isoprostane. This effect was abolished by SQ29,548. 8-iso-PGE2 displaced [3H]SQ29,548 or [125I]BOP binding in aortic smooth muscle cells with the affinity rank order of SQ29,548 > = I-BOP...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Fukunaga M,Takahashi K,Badr KF

    更新日期:1993-09-15 00:00:00

  • Fcf1p and Fcf2p are novel nucleolar Saccharomyces cerevisiae proteins involved in pre-rRNA processing.

    abstract::The uncharacterized Saccharomyces cerevisiae proteins Fcf1 and Fcf2, encoded by the ORFs YDR339c and YLR051c, respectively, were identified in a tandem affinity purification experiment of the known 40S factor Faf1p. Most of the proteins associated with TAP-Faf1p are trans-acting factors involved in pre-rRNA processing...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Rempola B,Karkusiewicz I,Piekarska I,Rytka J

    更新日期:2006-07-28 00:00:00

  • UT-B1 urea transporter plays a noble role as active water transporter in C6 glial cells.

    abstract::Since our experimental results suggest that UT-B1 functions as active water transporter against osmotic gradient in C6 glial cells, we report here for the first time the evidence for the active water transport. Exposure of C6 cells to a hyperosmotic solution containing glycerol or sucrose produced cell shrinkage due t...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Ogami A,Miyazaki H,Niisato N,Sugimoto T,Marunaka Y

    更新日期:2006-12-22 00:00:00