Osteoprotegerin in sputum is a potential biomarker in COPD.

Abstract:

BACKGROUND:COPD is characterized by chronic airflow limitation and inflammation of the respiratory tract. Several inflammatory biomarkers have been evaluated in COPD but are poorly related to disease severity and progression. Osteoprotegerin (OPG) is a glycoprotein mediator that is expressed in the lung and macrophages, so we have studied its concentration in induced sputum and macrophages of patients with COPD. METHODS:OPG was measured by enzyme-linked immunosorbent assay in induced sputum of patients with COPD and control subjects. RESULTS:OPG concentrations in induced sputum of patients with COPD (18.7 ± 18.6 ng/mL, n = 39) were significantly higher than those of healthy smokers (8.1 ± 5.6 ng/mL, n = 15), healthy nonsmokers (3.5 ± 3.8 ng/mL, n = 14), or patients with asthma (8.0 ± 5.4 ng/mL, n = 18). Sputum OPG levels in COPD negatively correlated with FEV(1) and positively correlated with residual volume to total lung capacity ratio (RV/TLC) (r = 0.55, P < .05), transfer factor of the lung for carbon monoxide (r = -0.53, P < .05), and carbon monoxide transfer coefficient (r = -0.61, P < .01). By contrast, sputum IL-8 concentrations were related to disease severity but not to RV/TLC or gas diffusion. Airway macrophages and neutrophils were positive for OPG by immunocytochemistry in sputum and peripheral lung tissue. OPG induced matrix metalloproteinase-9 release from sputum macrophages in vitro. CONCLUSIONS:Sputum OPG may be a useful biomarker to monitor parenchymal destruction in COPD.

journal_name

Chest

journal_title

Chest

authors

To M,Ito K,Ausin PM,Kharitonov SA,Barnes PJ

doi

10.1378/chest.10-1608

subject

Has Abstract

pub_date

2011-07-01 00:00:00

pages

76-83

issue

1

eissn

0012-3692

issn

1931-3543

pii

S0012-3692(11)60349-8

journal_volume

140

pub_type

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