Abstract:
BACKGROUND:Increased expression of the human epididymis protein 4 (HE4) was previously described in lung biopsy samples from patients with cystic fibrosis (CF). It remains unknown, however, whether serum HE4 concentrations are elevated in CF. METHODS:Seventy-seven children with CF from six Hungarian CF centers and 57 adult patients with CF from a Czech center were enrolled. In addition, 94 individuals with non-CF lung diseases and 117 normal control subjects with no pulmonary disorders were analyzed. Serum HE4 levels were measured by using an immunoassay, and their expression was further investigated via the quantification of HE4 messenger RNA by using quantitative reverse transcription polymerase chain reaction in CF vs non-CF respiratory epithelium biopsy specimens. The expression of the potential regulator miR-140-5p was analyzed by using an UPL-based quantitative reverse transcription polymerase chain reaction assay. HE4 was measured in the supernatants from unpolarized and polarized cystic fibrosis bronchial epithelial cells expressing wild-type or F508del-CFTR. RESULTS:Median serum HE4 levels were significantly elevated in children with CF (99.5 [73.1-128.9] pmol/L) compared with control subjects (36.3 [31.1-43.4] pmol/L; P < .0001). This observation was replicated in adults with CF (115.7 [77.8-148.7] pmol/L; P < .0001). In contrast, abnormal but lower HE4 concentrations were found in cases of severe bronchitis, asthma, pneumonia, and bronchiectasis. In patients with CF, the concentrations of HE4 were positively correlated with overall disease severity and C-reactive protein concentrations, whereas a significant inverse relationship was found between HE4 and the spirometric FEV1 value. Relative HE4 mRNA levels were significantly upregulated (P = .011) with a decreased miR-140-5p expression (P = .020) in the CF vs non-CF airway biopsy specimens. Twofold higher HE4 concentrations were recorded in the supernatant of polarized F508del-CF transmembrane conductance regulator/bronchial epithelial cells compared with wild-type cells. CONCLUSIONS:HE4 serum levels positively correlate with the overall severity of CF and the degree of pulmonary dysfunction. HE4 may thus be used as a novel inflammatory biomarker and possibly also as a measure of treatment efficacy in CF lung disease.
journal_name
Chestjournal_title
Chestauthors
Nagy B Jr,Nagy B,Fila L,Clarke LA,Gönczy F,Bede O,Nagy D,Újhelyi R,Szabó Á,Anghelyi A,Major M,Bene Z,Fejes Z,Antal-Szalmás P,Bhattoa HP,Balla G,Kappelmayer J,Amaral MD,Macek M Jr,Balogh Idoi
10.1016/j.chest.2016.04.006subject
Has Abstractpub_date
2016-09-01 00:00:00pages
661-72issue
3eissn
0012-3692issn
1931-3543pii
S0012-3692(16)48554-5journal_volume
150pub_type
杂志文章相关文献
CHEST文献大全abstract::The effect of combined administration of different dose ratios of dobutamine (DB) and dopamine (DA) (DB/DA ratio of 1:1; 1.5:0.5; 2:0; 0.5:1.5; and 0:2), with the added dose kept constant (10 micrograms/kg/min-20 micrograms/kg/min), on right ventricular function (measured by the thermal washout method with the aid of ...
journal_title:Chest
pub_type: 杂志文章
doi:10.1378/chest.101.5.1197
更新日期:1992-05-01 00:00:00
abstract:BACKGROUND:Cheyne-Stokes respiration (CSR) frequently occurs in patients with severe heart failure during sleep and may increase mortality. Daytime CSR supposedly poses an even greater risk, but its prevalence and prognostic importance remain elusive. Therefore, we investigated the circadian prevalence of CSR and its i...
journal_title:Chest
pub_type: 杂志文章
doi:10.1378/chest.07-0121
更新日期:2007-11-01 00:00:00
abstract:BACKGROUND:Club cell secretory protein (CCSP) is a protective biomarker associated with annual decline in lung function. COPD progression results from an imbalance between injury and repair initially triggered by cigarette smoking. OBJECTIVE:We investigated the effect of CCSP as a therapeutic strategy to restore the b...
journal_title:Chest
pub_type: 杂志文章
doi:10.1378/chest.14-1174
更新日期:2015-06-01 00:00:00
abstract::alpha 1-Antitrypsin (alpha 1-AT) accumulates in the rough endoplasmic reticulum through a mechanism of polymerization. Polymerization is favored by the incorrect tertiary structure of the alpha 1-AT caused by a point mutation at position 342 of the protein. Accumulation of alpha 1-AT in the liver cells (and in hepatoc...
journal_title:Chest
pub_type: 杂志文章,评审
doi:10.1378/chest.110.6_supplement.251s
更新日期:1996-12-01 00:00:00
abstract::Pancreaticopleural fistula secondary to chronic pancreatitis is a rare cause of recurrent pleural effusion. The demonstration of the fistula with endoscopic retrograde pancreatography and CT is invasive or limited. We report in two patients the use of magnetic resonance pancreatography as a noninvasive alternative to ...
journal_title:Chest
pub_type: 杂志文章
doi:10.1378/chest.117.3.912
更新日期:2000-03-01 00:00:00
abstract::Disputes regarding life-prolonging treatments are stressful for all parties involved. These disagreements are appropriately almost always resolved with intensive communication and negotiation. Those rare cases that are not require a resolution process that ensures fairness and due process. We describe three recent cas...
journal_title:Chest
pub_type: 杂志文章,评审
doi:10.1016/j.chest.2016.05.026
更新日期:2016-09-01 00:00:00
abstract:STUDY OBJECTIVES:Pocket hematoma is a common complication after pacemaker or implantable cardioverter defibrillator (ICD) implantation. Thus, we investigated the influence of patient comorbidity, implantation strategy, operator experience, antiplatelet therapy, and anticoagulation therapy on hematoma rate. DESIGN:Betw...
journal_title:Chest
pub_type: 杂志文章
doi:10.1378/chest.126.4.1177
更新日期:2004-10-01 00:00:00
abstract:STUDY OBJECTIVE:To compare drug output from a vented nebulizer (Pari LC Jet Plus) with a traditional unvented nebulizer (Hudson 1730 T Up-Draft 11) using aerosolized tobramycin, which is frequently used in the treatment of cystic fibrosis. DESIGN:Six nebulizers of each type were filled with a 4 mL tobramycin (80 mg) s...
journal_title:Chest
pub_type: 杂志文章
doi:10.1378/chest.113.4.951
更新日期:1998-04-01 00:00:00
abstract::A diagnosis of severe obstructive sleep apnea was made after a 52-year-old hypertensive man developed a large intracranial hemorrhage. Therapeutic noninvasive positive pressure ventilation (NPPV) for obstructive sleep apnea and hypoventilation was complicated by transient unilateral orbital herniation. As best as can ...
journal_title:Chest
pub_type: 杂志文章
doi:10.1378/chest.113.3.841
更新日期:1998-03-01 00:00:00
abstract:BACKGROUND:Balance deficits and an increased fall risk are well documented in individuals with COPD. Despite evidence that balance training programs can improve performance on clinical balance tests, their minimal clinically important difference (MCID) is unknown. The aim of this study was to determine the MCID of the ...
journal_title:Chest
pub_type: 杂志文章
doi:10.1378/chest.15-0717
更新日期:2016-03-01 00:00:00
abstract::We describe an unusual case of orthodeoxia platypnea syndrome exacerbated by right ventricular inflow obstruction due to iatrogenic steroid-induced adipose deposition in cardiac tissues. A 68-year-old man on long-term prednisone therapy for eosinophilic pneumonia presented with progressive dyspnea worsened by bending ...
journal_title:Chest
pub_type: 杂志文章
doi:10.1378/chest.10-0874
更新日期:2011-02-01 00:00:00
abstract:BACKGROUND:Central venous pressure (CVP) is used almost universally to guide fluid therapy in hospitalized patients. Both historical and recent data suggest that this approach may be flawed. OBJECTIVE:A systematic review of the literature to determine the following: (1) the relationship between CVP and blood volume, (...
journal_title:Chest
pub_type: 杂志文章,meta分析,评审
doi:10.1378/chest.07-2331
更新日期:2008-07-01 00:00:00
abstract::The effect of broxaterol, a new beta 2-agonist, on respiratory muscle endurance and strength was studied in a double-blind, placebo-controlled, randomized crossover clinical trial in 16 patients with chronic obstructive pulmonary disease (COPD) with irreversible airway obstruction (FEV1 = 57.1 percent of predicted). O...
journal_title:Chest
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1378/chest.101.1.133
更新日期:1992-01-01 00:00:00
abstract::We studied the correspondence between fluctuations of esophageal pressure measured before and after placement of a nasogastric (NG) tube in six normal volunteers. Flow, airway pressure, and esophageal pressure data from at least 20 breaths were recorded in seven ventilatory conditions in two body postures: 0 degree (s...
journal_title:Chest
pub_type: 杂志文章
doi:10.1378/chest.106.1.137
更新日期:1994-07-01 00:00:00
abstract::Dieulafoy's disease is a vascular anomaly characterized by the presence of a dysplastic artery that is related to an epithelial ulcer. While it is most frequently a GI condition, occurrence in the bronchus has been reported previously in six cases. We present the case of a 51-year-old man with severe hemoptysis and ep...
journal_title:Chest
pub_type: 杂志文章
doi:10.1378/chest.119.1.292
更新日期:2001-01-01 00:00:00
abstract::Present-day technology has greatly facilitated the monitoring of respiratory gas exchange in the clinical exercise laboratory. Despite the growing use of these techniques to assess the severity and progression of disease or therapeutic response in patients with heart failure, the long-term reproducibility of oxygen up...
journal_title:Chest
pub_type: 杂志文章
doi:10.1378/chest.97.1.12
更新日期:1990-01-01 00:00:00
abstract:CASE PRESENTATION:A 38-year-old man of Indian origin, who migrated to Greece 13 years prior to presentation, was admitted to our hospital with a 2-month history of nonprogressive, intermittent (mostly evening), low-grade (up to 38.5°C) fever, accompanied by night sweats, dry cough, mild dyspnea on exertion (modified Me...
journal_title:Chest
pub_type: 杂志文章
doi:10.1016/j.chest.2018.05.012
更新日期:2018-10-01 00:00:00
abstract::We tested the response characteristics of a dual transcutaneous (tc) PO2/PCO2 monitoring system in healthy subjects who breathed various gas mixtures, and we compared steady-state tc readings to simultaneous arterial blood gas analysis in 20 stable respiratory outpatients. The electrodes were simple to apply, required...
journal_title:Chest
pub_type: 杂志文章
doi:10.1378/chest.99.5.1211
更新日期:1991-05-01 00:00:00
abstract:BACKGROUND:Studies of the impact of respiratory muscle training (RMT) on central neurodegenerative pathologies have been aimed at improving pulmonary function. However, there is no certainty about the effectiveness of RMT in patients affected by these groups of disorders. The purpose of this review was to assess the ev...
journal_title:Chest
pub_type: 杂志文章,评审
doi:10.1378/chest.12-1442
更新日期:2013-05-01 00:00:00
abstract::Pulmonary embolism (PE) is a common disorder that is accompanied by significant morbidity and mortality. Although anticoagulation is the standard treatment for PE, thrombolytic therapy, with its ability to produce rapid clot lysis, has long been considered an attractive alternative. Although many studies have been per...
journal_title:Chest
pub_type: 杂志文章,评审
doi:10.1378/chest.115.6.1695
更新日期:1999-06-01 00:00:00
abstract::Percutaneous cannulation of the internal jugular vein is commonly performed to obtain central venous access. We report the first case of massive hemoptysis occurring during cannulation of the internal jugular vein. ...
journal_title:Chest
pub_type: 杂志文章
doi:10.1378/chest.95.5.1143
更新日期:1989-05-01 00:00:00
abstract::A 32-year-old man was admitted to the emergency department of our hospital after experiencing a peripheral arterial embolism. Investigation of the possible embolic sources in an otherwise asymptomatic patient revealed the existence of a left ventricular diverticulum. The left ventricular diverticulum is a rare congeni...
journal_title:Chest
pub_type: 杂志文章
doi:10.1378/chest.127.4.1452
更新日期:2005-04-01 00:00:00
abstract::Twelve adult patients with ostium primum atrial septal defects (incomplete endocardial cushion defect) who underwent surgical repair of their lesions were evaluated in the late postoperative period. All had closure of the low-lying atrial septal defect, with suturing of the mitral valve cleft in 11 patients. Although ...
journal_title:Chest
pub_type: 杂志文章
doi:10.1378/chest.67.2.185
更新日期:1975-02-01 00:00:00
abstract::We report the findings in a patient with Klippel-Feil syndrome and associated agenesis of right upper and middle lobes, hypoplasia of the right lower lobe of the lung, and Lown-Ganong-Levine syndrome. To our knowledge, such an association has not been previously described. ...
journal_title:Chest
pub_type: 杂志文章
doi:10.1378/chest.101.4.1157
更新日期:1992-04-01 00:00:00
abstract:BACKGROUND:We evaluated the association between hemodynamic parameters of chronic congestive heart failure (CHF) and mediastinal lymphadenopathy (MLA) in heart transplantation (HT) candidates and the effect of HT on MLA. We also described the results of lymph node (LN) biopsies of MLA in the patients. METHODS:Patients...
journal_title:Chest
pub_type: 杂志文章
doi:10.1378/chest.10-1386
更新日期:2011-06-01 00:00:00
abstract:BACKGROUND:Studies have demonstrated that high-flow nasal cannula (HFNC) prevents intubation in acute hypoxic respiratory failure when compared with conventional oxygen therapy (COT). However, the data examining routine HFNC use in the immediate postoperative period are less clear. RESEARCH QUESTION:Is routine HFNC us...
journal_title:Chest
pub_type: 杂志文章
doi:10.1016/j.chest.2020.06.038
更新日期:2020-11-01 00:00:00
abstract:STUDY OBJECTIVE:To evaluate the incidence and clinical factors related to the persistence of infarct-associated pericardial effusion (PE) after primary angioplasty. DESIGN:Consecutive case-series analysis. SETTING:Coronary care unit in a university hospital. PATIENTS:Three hundred ninety-one consecutive patients wit...
journal_title:Chest
pub_type: 杂志文章
doi:10.1378/chest.128.2.798
更新日期:2005-08-01 00:00:00
abstract::This report describes a patient who developed pulmonary hypertension 6 years after lung transplantation for primary pulmonary hypertension (PPH). Evaluation with right heart catheterization followed by pulmonary angiography, however, demonstrated that the pulmonary hypertension was secondary to an anastomotic narrowin...
journal_title:Chest
pub_type: 杂志文章
doi:10.1378/chest.116.2.564
更新日期:1999-08-01 00:00:00
abstract::This study evaluated the influence of pregnancy as a relapse factor in pulmonary tuberculosis in 215 patients who recieved adequate chemotherapy. A comparison was made between the evolution of patients who had pregnancies and those who did not. On ending the period of observation (details are lacking with respect to o...
journal_title:Chest
pub_type: 杂志文章
doi:10.1378/chest.68.6.800
更新日期:1975-12-01 00:00:00
abstract::In an effort to characterize the effectiveness of cardiology consultation, the outcomes of consultants' recommendations for diagnostic actions and cardiac drugs were quantitatively examined in 394 cases. Drug recommendations were made more frequently (49 percent) than diagnostic recommendations (38 percent) and were a...
journal_title:Chest
pub_type: 杂志文章
doi:10.1378/chest.79.1.16
更新日期:1981-01-01 00:00:00