Abstract：
:Functional lox-like sequences have been identified within the yeast and mammalian genome. These hetero-specific lox sites also allow Cre recombinase to specifically target efficient integration of exogenous DNA into the endogenous pseudo-lox (ψlox) sequences that occur naturally in the host genome using a Cre/loxP integrative recombination system. We investigated whether the Cre/ψlox system is useful for site-specific integration of transgenes and for improving the production efficiency of transgenic animals. This is the first report on Cre-mediated integrative recombination targeting an endogenous lox-like sequence termed pseudo-loxm5 (ψloxm5) in early mouse embryos. We characterized the Cre/ψloxm5 system in embryonic environment. Cre-expressing plasmid and a transgene (CMV/LacZ gene) flanked by ψloxm5 and ψloxcorem5 sites were co-microinjected into the pronucleus of fertilized mouse oocytes. The injected eggs were transferred into foster mothers, and the recombination products were investigated. The results show that the ψloxm5 site is an active substrate for Cre-mediated recombination in the mouse embryonic environment. The transgenesis efficiency was up to 27% (6/22). The site-specific integration of the transgene into the endogenous ψloxm5 site was found in 50 % of the transgenic pups. Our findings demonstrated that the Cre/ψloxm5 integrative recombination system is an efficient and simple strategy for targeting an endogenous lox-like site in mammalian embryos.
journal_name
J Appl Genetjournal_title
Journal of applied geneticsauthors
Ito M,Yamanouchi K,Naito K,Calos MP,Tojo Hdoi
10.1007/s13353-010-0011-3subject
Has Abstractpub_date
2011-02-01 00:00:00pages
89-94issue
1eissn
1234-1983issn
2190-3883journal_volume
52pub_type
杂志文章abstract：:The Npc1nih/nih-null model and the Npc1nmf164/nmf164 hypomorph models of Niemann-Pick C1 (NPC1) disease show defects in olfaction. We have tested the effects of the life-prolonging treatment hydroxypropyl-beta-cyclodextrin (HPBCD) on olfaction and neural stem cell numbers when delivered either systemically or by nasal...
journal_title：Journal of applied genetics
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journal_title：Journal of applied genetics
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更新日期：2004-01-01 00:00:00
abstract：:Phenylketonuria (PKU) is a metabolic disease caused by mutations in the phenylalanine hydroxylase (PAH) gene. Although the PAH genotype remains the main determinant of PKU phenotype severity, genotype-phenotype inconsistencies have been reported. In this study, we focused on unanalysed sequences in non-coding PAH gene...
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journal_title：Journal of applied genetics
pub_type： 杂志文章,评审
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pub_type： 杂志文章,评审
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journal_title：Journal of applied genetics
pub_type： 杂志文章
doi：10.1007/s13353-017-0422-5
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journal_title：Journal of applied genetics
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doi：10.1007/BF03195698
更新日期：2009-01-01 00:00:00
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journal_title：Journal of applied genetics
pub_type： 杂志文章
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journal_title：Journal of applied genetics
pub_type： 杂志文章,评审
doi：
更新日期：2004-01-01 00:00:00
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journal_title：Journal of applied genetics
pub_type： 杂志文章
doi：10.1007/s13353-014-0233-x
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journal_title：Journal of applied genetics
pub_type： 杂志文章
doi：10.1007/BF03194645
更新日期：2006-01-01 00:00:00
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journal_title：Journal of applied genetics
pub_type： 杂志文章
doi：10.1007/BF03195664
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journal_title：Journal of applied genetics
pub_type： 杂志文章
doi：
更新日期：2004-01-01 00:00:00
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pub_type： 杂志文章,评审
doi：10.1007/s13353-016-0380-3
更新日期：2017-08-01 00:00:00
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journal_title：Journal of applied genetics
pub_type： 杂志文章
doi：
更新日期：2001-01-01 00:00:00
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doi：
更新日期：2001-01-01 00:00:00
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journal_title：Journal of applied genetics
pub_type： 杂志文章,评审
doi：
更新日期：2002-01-01 00:00:00
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journal_title：Journal of applied genetics
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doi：
更新日期：2003-01-01 00:00:00
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pub_type： 杂志文章
doi：10.1007/s13353-016-0345-6
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journal_title：Journal of applied genetics
pub_type： 杂志文章
doi：10.1007/BF03208872
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journal_title：Journal of applied genetics
pub_type： 杂志文章
doi：10.1007/BF03194656
更新日期：2007-01-01 00:00:00
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journal_title：Journal of applied genetics
pub_type： 杂志文章
doi：10.1007/BF03195642
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journal_title：Journal of applied genetics
pub_type： 杂志文章
doi：
更新日期：2001-01-01 00:00:00
abstract：:Philomycus bilineatus is a highly common gastropod mollusk pest in China and is also utilized to treat infectious diseases. However, no genomic resources are available for this non-model species. In the present study, the transcriptomic analysis of P. bilineatus was completed. After sequencing using the next generatio...
journal_title：Journal of applied genetics
pub_type： 杂志文章
doi：10.1007/s13353-020-00566-4
更新日期：2020-09-01 00:00:00
abstract：:Hybrids derived from wheat (Triticum aestivum L.) × rye (Secale cereale L.) have been widely studied because of their important roles in wheat cultivar improvement. Repetitive sequences pAs1, pSc119.2, pTa-535, pTa71, CCS1, and pAWRC.1 are usually used as probes in fluorescence in situ hybridization (FISH) analysis of...
journal_title：Journal of applied genetics
pub_type： 杂志文章
doi：10.1007/s13353-014-0215-z
更新日期：2014-08-01 00:00:00
