Abstract:
OBJECTIVE:To determine the role of hepatocyte growth factor (HGF)/c-Met on β-cell survival in diabetogenic conditions in vivo and in response to cytokines in vitro. RESEARCH DESIGN AND METHODS:We generated pancreas-specific c-Met-null (PancMet KO) mice and characterized their response to diabetes induced by multiple low-dose streptozotocin (MLDS) administration. We also analyzed the effect of HGF/c-Met signaling in vitro on cytokine-induced β-cell death in mouse and human islets, specifically examining the role of nuclear factor (NF)-κB. RESULTS:Islets exposed in vitro to cytokines or from MLDS-treated mice displayed significantly increased HGF and c-Met levels, suggesting a potential role for HGF/c-Met in β-cell survival against diabetogenic agents. Adult PancMet KO mice displayed normal glucose and β-cell homeostasis, indicating that pancreatic c-Met loss is not detrimental for β-cell growth and function under basal conditions. However, PancMet KO mice were more susceptible to MLDS-induced diabetes. They displayed higher blood glucose levels, marked hypoinsulinemia, and reduced β-cell mass compared with wild-type littermates. PancMet KO mice showed enhanced intraislet infiltration, islet nitric oxide (NO) and chemokine production, and β-cell apoptosis. c-Met-null β-cells were more sensitive to cytokine-induced cell death in vitro, an effect mediated by NF-κB activation and NO production. Conversely, HGF treatment decreased p65/NF-κB activation and fully protected mouse and, more important, human β-cells against cytokines. CONCLUSIONS:These results show that HGF/c-Met is critical for β-cell survival by attenuating NF-κB signaling and suggest that activation of the HGF/c-Met signaling pathway represents a novel strategy for enhancing β-cell protection.
journal_name
Diabetesjournal_title
Diabetesauthors
Mellado-Gil J,Rosa TC,Demirci C,Gonzalez-Pertusa JA,Velazquez-Garcia S,Ernst S,Valle S,Vasavada RC,Stewart AF,Alonso LC,Garcia-Ocaña Adoi
10.2337/db09-1305subject
Has Abstractpub_date
2011-02-01 00:00:00pages
525-36issue
2eissn
0012-1797issn
1939-327Xpii
db09-1305journal_volume
60pub_type
杂志文章相关文献
DIABETES文献大全abstract::During epidemiologic investigations of vascular complications of diabetes in Warsaw the habit of cigarette smoking was analyzed in the subjects studied (4,530 diabetics). It was found that in the group of diabetics the number of ex-smokers was much greater (p less than 0.01) and that of present smokers lower (p less t...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diab.25.8.717
更新日期:1976-08-01 00:00:00
abstract::Glucose stimulates insulin secretion by generating triggering and amplifying signals in beta-cells. The triggering pathway is well characterized. It involves the following sequence of events: entry of glucose by facilitated diffusion, metabolism of glucose by oxidative glycolysis, rise in the ATP-to-ADP ratio, closure...
journal_title:Diabetes
pub_type: 杂志文章,评审
doi:10.2337/diabetes.49.11.1751
更新日期:2000-11-01 00:00:00
abstract::The influence of insulin on the intracellular free sodium and potassium ion concentrations ([Na+]i, [K+]i) and resting membrane potential of rat epididymal adipocytes was examined to determine its potential for mediating insulin's action on other cellular processes. Direct intracellular measurements of [Na+]i, [K+]i, ...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diab.29.12.1040
更新日期:1980-12-01 00:00:00
abstract::Alterations in dopaminergic circuitry play a critical role in food reward and may contribute to susceptibility to obesity. Ingestion of sweets releases dopamine in striatum, and both sweet preferences and striatal D2 receptors (D2R) decline with age and may be altered in obesity. Understanding the relationships betwee...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/db16-0407
更新日期:2016-09-01 00:00:00
abstract::Diabetes diagnostic therapy and research would strongly benefit from noninvasive accurate imaging of the functional β-cells in the pancreas. Here, we developed an analysis of functional β-cell mass (BCM) by measuring manganese (Mn(2+)) uptake kinetics into glucose-stimulated β-cells by T1-weighted in vivo Mn(2+)-media...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/db14-0864
更新日期:2015-06-01 00:00:00
abstract::Islet antigens associated with type 1 diabetes include a recently identified protein tyrosine phosphatase-like molecule IA-2, which contains the intracellular fragment IA-2ic. To determine whether combinations of antibodies including those to IA-2 characterize and predict type 1 diabetes, we studied antibodies to IA-2...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diab.46.8.1270
更新日期:1997-08-01 00:00:00
abstract::Mice genetically deficient in the glucagon receptor (Gcgr(-/-)) show improved glucose tolerance, insulin sensitivity, and α-cell hyperplasia. In addition, Gcgr(-/-) mice do not develop diabetes after chemical destruction of β-cells. Since fibroblast growth factor 21 (FGF21) has insulin-independent glucose-lowering pro...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/db13-0710
更新日期:2014-01-01 00:00:00
abstract::Leptin administration has been shown to increase renal, adrenal, and lumbar sympathetic nerve activity. However, this generalized sympathoexcitatory activity is not always followed by an increase in arterial pressure. The present study tested the hypothesis that leptin induces a release of nitric oxide (NO) that oppos...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diabetes.48.4.903
更新日期:1999-04-01 00:00:00
abstract::A key pathophysiologic role for activated T-cells in mediating adipose inflammation and insulin resistance (IR) has been recently postulated. However, mechanisms underlying their activation are poorly understood. In this study, we demonstrated a previously unrecognized homeostatic role for the costimulatory B7 molecul...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/db13-1094
更新日期:2014-04-01 00:00:00
abstract::In evaluating therapeutic interventions aimed at preventing diabetic neuropathy, choosing a suitable measure of neural function is difficult, partly because the relation between most available objective measures and the development of symptomatic neuropathy (SN) is unclear. Using data from 67 diabetic patients, we dev...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diab.35.8.873
更新日期:1986-08-01 00:00:00
abstract::Therapeutic engineering of glucagon-like peptide 1 (GLP-1) has enabled development of new medicines to treat type 2 diabetes. These injectable analogs achieve robust glycemic control by increasing concentrations of "GLP-1 equivalents" (∼50 pmol/L). Similar levels of endogenous GLP-1 occur after gastric bypass surgery,...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/db17-0607
更新日期:2018-02-01 00:00:00
abstract::We have recently reported successful 72-h preservation of the canine pancreas with a new cold-storage solution developed at the University of Wisconsin (UW solution). Over 10 mo, we performed 11 combined pancreas-kidney and 4 isolated-pancreas transplants with this solution. In situ cooling of the donor pancreas was p...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diab.38.1.s7
更新日期:1989-01-01 00:00:00
abstract:OBJECTIVE:Anti-tissue transglutaminase (TG2) antibodies are the serological marker of celiac disease. Given the close association between celiac disease and type 1 diabetes, we investigated the production and deposition of anti-TG2 antibodies in the jejunal mucosa of type 1 diabetic children. RESEARCH DESIGN AND METHO...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/db08-0962
更新日期:2009-07-01 00:00:00
abstract::Peroxisome proliferator-activated receptor (PPAR)gamma is a key transcription factor facilitating fat deposition in adipose tissue through its proadipogenic and lipogenic actions. Human patients with dominant-negative mutations in PPARgamma display lipodystrophy and extreme insulin resistance. For this reason it was c...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/db06-0389
更新日期:2006-10-01 00:00:00
abstract::The role of interferons, either pathogenic or protective, during autoimmune diabetes remains controversial. Herein, we examine the progression of diabetes in NOD mice lacking the type I (IFNAR) or type II (IFNGR) interferon receptor and, for the first time, in mice deficient in both receptors (double knockout [DKO]). ...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/db18-0331
更新日期:2018-09-01 00:00:00
abstract::Nearly 20 years ago, it was suggested that individuals exist who are not obese on the basis of height and weight, but who, like people with overt obesity, are hyperinsulinemic, insulin-resistant, and predisposed to type 2 diabetes, hypertriglyceridemia, and premature coronary heart disease. Since then it has become in...
journal_title:Diabetes
pub_type: 杂志文章,评审
doi:10.2337/diabetes.47.5.699
更新日期:1998-05-01 00:00:00
abstract::Linkage results suggest that the region of chromosome 7 containing the leptin gene cosegregates with extreme obesity; however, leptin coding region mutations are rare. To investigate whether the leptin flanking sequence and/or a larger 40-cM region (7q22.1-7q35) contributes to obesity, we genotyped individuals from 20...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diabetes.52.6.1557
更新日期:2003-06-01 00:00:00
abstract::Diabetes-induced oxidative stress can lead to protein misfolding and degradation by the ubiquitin-proteasome system. This study examined protein ubiquitination in pancreatic sections from Zucker diabetic fatty rats. We observed large aggregates of ubiquitinated proteins (Ub-proteins) in insulin-expressing beta-cells a...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/db06-1160
更新日期:2007-04-01 00:00:00
abstract::Impaired wound healing is one of the main causes of diabetic foot ulcerations. However, the exact mechanism of delayed wound healing in diabetes is not fully understood. Long noncoding RNAs (lncRNAs) are widely involved in a variety of biological processes and diseases, including diabetes and its associated complicati...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/db20-0147
更新日期:2020-10-01 00:00:00
abstract:OBJECTIVE:Visceral obesity and elevated plasma free fatty acids are predisposing factors for type 2 diabetes. Chronic exposure to these lipids is detrimental for pancreatic beta-cells, resulting in reduced insulin content, defective insulin secretion, and apoptosis. We investigated the involvement in this phenomenon of...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/db07-1252
更新日期:2008-10-01 00:00:00
abstract::Mutations in the LMNA gene (encoding lamin A/C) underlie familial partial lipodystrophy, a syndrome of monogenic insulin resistance and diabetes. LMNA maps to the well-replicated diabetes-linkage region on chromosome 1q, and there are reported associations between LMNA single nucleotide polymorphisms (SNPs) (particula...
journal_title:Diabetes
pub_type: 杂志文章,meta分析
doi:10.2337/db06-0930
更新日期:2007-03-01 00:00:00
abstract::Sirtuin 1 (SIRT1), an NAD(+)-dependent protein deacetylase, regulates a host of target proteins, including peroxisome proliferator-activated receptor (PPAR)-γ coactivator-1α (PGC-1α), a transcriptional coregulator that binds to numerous transcription factors in response to deacetylation to promote mitochondrial biogen...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/db14-0325
更新日期:2015-02-01 00:00:00
abstract::Patient-derived progenitor cell (PC) dysfunction is severely impaired in diabetes, but the molecular triggers that contribute to mechanisms of PC dysfunction are not fully understood. Methylglyoxal (MGO) is one of the highly reactive dicarbonyl species formed during hyperglycemia. We hypothesized that the MGO scavenge...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/db18-0933
更新日期:2019-06-01 00:00:00
abstract::Bcl-2 mRNA expression was detected in rat blastocysts by in situ hybridization. The distribution of mRNA expression was rather heterogenous, with approximately 2% of high-expressing cells. In vitro exposure to 28 mmol/l D-glucose for 24 h resulted in a significant increase in the proportion of these cells compared wit...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diabetes.50.1.143
更新日期:2001-01-01 00:00:00
abstract::Type 1 diabetes is caused by adaptive immune responses, but innate immunity is important because monocytes infiltrate islets. Activated monocytes express cyclooxygenase (COX)-2, promoting prostaglandin-E(2) (PGE(2)) secretion, whereas COX-1 expression is constitutive. We aimed to define monocyte COX expression in type...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/db06-0447
更新日期:2006-12-01 00:00:00
abstract::Transracial analysis provides a method of distinguishing primary associations between insulin-dependent diabetes mellitus (IDDM) and HLA class II alleles from those secondary to linkage disequilibrium. Blacks show DR-DQ relationships that are different from other races and are a useful group in which to investigate HL...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diab.40.6.748
更新日期:1991-06-01 00:00:00
abstract::This cross-sectional study evaluated the relationship between 1) functional and structural measurements of neurodegeneration in the initial stages of diabetic retinopathy (DR) and 2) the presence of neurodegeneration and early microvascular impairment. We analyzed baseline data of 449 patients with type 2 diabetes enr...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/db16-1453
更新日期:2017-09-01 00:00:00
abstract::Embryonic stem (ES) cells display the ability to differentiate in vitro into a variety of cell lineages. Using a cell-trapping system, we have obtained an insulin-secreting cell clone from undifferentiated ES cells. The construction used allows the expression of a neomycin selection system under the control of the reg...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diabetes.49.2.157
更新日期:2000-02-01 00:00:00
abstract:OBJECTIVE:Leukocyte infiltration of adipose is a critical determinant of obesity-related metabolic diseases. Fractalkine (CX3CL1) and its receptor (CX3CR1) comprise a chemokine system involved in leukocyte recruitment and adhesion in atherosclerosis, but its role in adipose inflammation and type 2 diabetes is unknown. ...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/db10-0956
更新日期:2011-05-01 00:00:00
abstract::Cytotoxic T lymphocytes (CTLs) constitute a major effector population in pancreatic islets from patients suffering from type 1 diabetes (T1D) and thus represent attractive targets for intervention. Some studies have suggested that blocking the interaction between the chemokine CXCL10 and its receptor CXCR3 on activate...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/db12-1370
更新日期:2013-07-01 00:00:00