Abstract:
:N(6)-isopentenyladenosine (i6A) inhibits the tumor cell growth by inducing cell apoptosis in various cancer cell lines. However, little is known regarding the mechanisms by which the drug induces cell apoptosis. In this study, we further explored the molecular mechanisms of i6A as an anticancer agent on a human breast cancer cell line MDA MB 231. Treatment with i6A decreased the cell proliferation of MDA MB 231 cells in a dose-dependent manner by arresting the cells at G(0)/G(1) phase. This effect was strongly associated with concomitant decrease in the level of cyclin D1, cyclin E, cdk2, and increase of p21waf1 and p27kip. In addition i6A also induced apoptotic cell death by increasing the expression of Bax, and decreasing the levels of Bcl-2 and Bcl-xL, and subsequently triggered mitochondria apoptotic pathway (release of cytochrome c and activation of caspase-3). We observed that i6A suppressed the nuclear factor kappaB (NF-κB) pathway and inhibited the Akt activation. The results of this study indicate that i6A decreases cell proliferation and induces apoptotic cell death in human breast cancer cells, possibly by decreasing signal transduction through the Akt/NF-κB cell survival pathway.
journal_name
Mol Carcinogjournal_title
Molecular carcinogenesisauthors
Laezza C,Malfitano AM,Di Matola T,Ricchi P,Bifulco Mdoi
10.1002/mc.20666subject
Has Abstractpub_date
2010-10-01 00:00:00pages
892-901issue
10eissn
0899-1987issn
1098-2744journal_volume
49pub_type
杂志文章abstract::Gastrointestinal stromal tumor (GIST) is a common mesenchymal tumor in the gastrointestinal tract. Prostate cancer associated transcript 6 (PCAT6) is a long noncoding RNA (lncRNA) and plays a pivotal role in tumor formation. Present study was designed to explore the function of PCAT6 in GIST. Ki67 staining, colony for...
journal_title:Molecular carcinogenesis
pub_type: 杂志文章
doi:10.1002/mc.23199
更新日期:2020-06-01 00:00:00
abstract::Apigenin (4',5,7-trihydroxyflavone), a flavone subclass of flavonoid widely distributed in many herbs, fruits, and vegetables is a substantial component of the human diet and has been shown to possess a variety of biological activities including tumor growth inhibition and chemoprevention. Recent studies in several bi...
journal_title:Molecular carcinogenesis
pub_type: 杂志文章
doi:10.1002/mc.20115
更新日期:2005-09-01 00:00:00
abstract::2-Methoxyestradiol (2-ME), an endogenous metabolite of 17beta-estradiol, is present in human blood and urine. Here we show for the first time that 2-ME significantly inhibited the growth of normal prostate epithelial cells and androgen-dependent LNCaP and androgen-independent DU145 prostate cancer cells. This growth i...
journal_title:Molecular carcinogenesis
pub_type: 杂志文章
doi:10.1002/mc.1046
更新日期:2001-07-01 00:00:00
abstract::Gap junctions are cellular structures which permit direct exchanges of small molecules from cytoplasm to cytoplasm in most of the cells of metazoan organisms. For four decades, it has been observed that the inhibition of this type of intercellular communication is often associated with tumorigenesis. The assumption th...
journal_title:Molecular carcinogenesis
pub_type: 杂志文章
doi:10.1002/mc.20393
更新日期:2008-05-01 00:00:00
abstract::Oncoprotein p28(GANK), overexpressed in hepatocellular carcinomas (HCC), binds to RelA and retains NF-κB in the cytoplasm to suppress NF-κB transactivation. However, the mechanism has not yet been elucidated. In this study, we clarified the mechanism of NF-κB regulated by p28(GANK). p28(GANK) reduced TNF-α-induced nuc...
journal_title:Molecular carcinogenesis
pub_type: 杂志文章
doi:10.1002/mc.22235
更新日期:2015-12-01 00:00:00
abstract::The E5 open reading frame (ORF) from bovine papillomavirus type 1 (BPV 1) as well as the E5 ORFs from human papillomaviruses (HPV) type 6 and type 16 have been reported to transform immortalized rodent cells. In an analysis of murine and human tumors for the presence of putative papillomavirus-related sequences, we cl...
journal_title:Molecular carcinogenesis
pub_type: 杂志文章
doi:10.1002/mc.2940060204
更新日期:1992-01-01 00:00:00
abstract::The P53 gene is a tumor suppressor gene and can prevent mutation and tumor induction though apoptosis and DNA repair when it is activated by genotoxic stress. miR-34a expression is regulated by the P53 gene and might be required for cell response to DNA damage. TK6 cells are human lymphoblast cells with normal P53 fun...
journal_title:Molecular carcinogenesis
pub_type: 杂志文章
doi:10.1002/mc.20830
更新日期:2012-08-01 00:00:00
abstract::Tumor-associated stromal cells are dynamic characters that endorse the carcinogenic process in a multitude of ways. The tumor microenvironment plays a crucial role throughout the tumor progression, which includes initiation, growth, invasion, and metastasis. The tumor microenvironment consists of cellular and non-cell...
journal_title:Molecular carcinogenesis
pub_type: 杂志文章,评审
doi:10.1002/mc.22583
更新日期:2017-04-01 00:00:00
abstract::The interplay between cancer cells and the normal surrounding tissue is believed to influence the biological behavior of the tumor. However, the presence of multiple cell types within the prelevated tumor specimen may attenuate changes that occur specifically in the malignant cells within their microenvironment. To st...
journal_title:Molecular carcinogenesis
pub_type: 杂志文章
doi:10.1002/mc.20055
更新日期:2004-12-01 00:00:00
abstract::Heterocyclic amines present in cooked foods are known to produce colon tumors in F344 rats at a high incidence, indicating the possibility of involvement of ras gene activation in colon carcinogenesis in rats as in humans. We examined mutations at codons 12, 13, and 61 of the Ki-ras, Ha-ras, and N-ras genes by polymer...
journal_title:Molecular carcinogenesis
pub_type: 杂志文章
doi:10.1002/mc.2940080110
更新日期:1993-01-01 00:00:00
abstract::Ajoene is a natural allylsulfur compound found in crushed garlic that arrests growth and induces apoptosis in cancer cells. To gain mechanistic insights into the cytotoxicity of ajoene in cancer cells, two fluorescently labelled ajoene analogs with dansyl- (DP) and fluorescein- (FOX) tags were synthesized. The tagged ...
journal_title:Molecular carcinogenesis
pub_type: 杂志文章
doi:10.1002/mc.22364
更新日期:2016-08-01 00:00:00
abstract::The expression of members of the family of matrix-degrading metalloproteinases (MMPs) is believed to contribute to the complex process of invasion and metastasis. In this study, specific cDNA probes for three members of the stromelysin subfamily of MMPs--stromelysin (MMP-3), stromelysin-2 (MMP-10), and pump-1 (MMP-7)-...
journal_title:Molecular carcinogenesis
pub_type: 杂志文章
doi:10.1002/mc.2940040617
更新日期:1991-01-01 00:00:00
abstract::microRNAs (miRNA) silence target genes through Watson-Crick based binding to the 3'untranslated regions (3'UTR). Thus, polymorphisms in the miRNA-binding sites may disrupt this process and play a potential role in cancer pathogenesis. Integrins have been implicated in the genesis and development of many tumors. This s...
journal_title:Molecular carcinogenesis
pub_type: 杂志文章
doi:10.1002/mc.21973
更新日期:2014-04-01 00:00:00
abstract::Genotoxicity tests indicate methanol (MeOH) is not mutagenic, but a rodent study has suggested carcinogenic potential, which could result from free radical-initiated oxidative DNA damage. To investigate this possibility we treated male CD-1 mice, New Zealand white rabbits, and cynomolgus monkeys with MeOH (2.0 g/kg ip...
journal_title:Molecular carcinogenesis
pub_type: 杂志文章
doi:10.1002/mc.20701
更新日期:2011-03-01 00:00:00
abstract::Angiogenesis is a prerequisite for progression of cancers. The number of genes linked to angiogenesis suggests the existence of complex gene-networks, which remain to be elucidated. To identify angiogenesis genes deregulated in carcinomas, we performed a meta-profiling analysis of published gene expression microarray ...
journal_title:Molecular carcinogenesis
pub_type: 杂志文章,meta分析
doi:10.1002/mc.20874
更新日期:2013-01-01 00:00:00
abstract::Chromosomal double-strand breaks (DSBs) occurring in mammalian cells can initiate genomic instability, and their misrepairs result in chromosomal deletion, amplification, and translocation, common findings in human tumors. The tumor-suppressor protein p53 is involved in maintaining genomic stability. In this study, we...
journal_title:Molecular carcinogenesis
pub_type: 杂志文章
doi:10.1002/1098-2744(200008)28:4<203::aid-mc3>3.0.co;
更新日期:2000-08-01 00:00:00
abstract::To determine if hemizygous transgenic mice carrying the human c-Ha-ras gene (CB6F1-Tg Hras2 mice (Hras2 mice)) are susceptible to the carcinogenic potential of known murine carcinogens, male and female Hras2 mice and their non-transgenic CB6F1 littermates (non-Tg mice) were each given a single intraperitoneal injectio...
journal_title:Molecular carcinogenesis
pub_type: 杂志文章
doi:10.1002/(sici)1098-2744(199711)20:3<298::aid-mc6>3
更新日期:1997-11-01 00:00:00
abstract::Tamoxifen (TAM) is widely used in the treatment and prevention of breast cancer. There is clear evidence that cytochrome P450 (CYP) 3A enzymes play an important role in TAM metabolism, resulting in metabolites that lead to formation of TAM-DNA adducts. We have investigated the effect of CYP3A2 antisense (AVI-4472) exp...
journal_title:Molecular carcinogenesis
pub_type: 杂志文章
doi:10.1002/mc.20143
更新日期:2006-02-01 00:00:00
abstract::Exposure to aflatoxin B(1) (AFB(1)) is one of the risk factors for developing hepatoma. In rats, activation of the ras gene is a prevalent event in AFB(1)-induced hepatocarcinogenesis. It is not clear whether a similar event occurs in humans. By analysis of codon 249 of the p53 gene, six of 36 human hepatoma samples w...
journal_title:Molecular carcinogenesis
pub_type: 杂志文章
doi:10.1002/(sici)1098-2744(199910)26:2<69::aid-mc1>3.
更新日期:1999-10-01 00:00:00
abstract::In rats, gamma-glutamyl transpeptidase (gamma GT) exists as a single-copy gene, and three distinct species of RNA (types I, II, and III) that differ in their 5' untranslated regions have been identified. To compare steady-state levels of these gamma GT RNAs in rat tissues, hepatic carcinomas, and cultured cells, we us...
journal_title:Molecular carcinogenesis
pub_type: 杂志文章
doi:10.1002/mc.2940050112
更新日期:1992-01-01 00:00:00
abstract::In the present study, we evaluated the effect of deleting Twist1 on keratinocyte proliferation and on skin tumor development using the two-stage chemical carcinogenesis model. BK5.Cre × Twist1(flox/flox) mice, which have a keratinocyte-specific Twist1 knockout (Twist1 KO), developed significantly reduced numbers of pa...
journal_title:Molecular carcinogenesis
pub_type: 杂志文章
doi:10.1002/mc.22335
更新日期:2016-05-01 00:00:00
abstract::Sel-1-like (SEL1L) is a putative tumor suppressor gene that is significantly downregulated in human pancreatic ductal adenocarcinoma (PDA). The mechanism of the downregulation is unclear. Here, we investigated whether aberrantly upregulated microRNAs (miRNAs) repressed the expression of SEL1L. From reported miRNA micr...
journal_title:Molecular carcinogenesis
pub_type: 杂志文章
doi:10.1002/mc.22023
更新日期:2014-09-01 00:00:00
abstract::To investigate the importance of oncogenes and tumor suppressor genes in bladder carcinogenesis, we determined the status of the expression of the MDM-2 and p53 genes and genetic alterations in the p53 gene in five bladder carcinoma cell lines and one kidney urothelial carcinoma cell line. Overexpression of MDM-2 mRNA...
journal_title:Molecular carcinogenesis
pub_type: 杂志文章
doi:10.1002/mc.2940130307
更新日期:1995-07-01 00:00:00
abstract::MAP1S (originally named C19ORF5) is a widely distributed homolog of neuronal-specific MAP1A and MAP1B, and bridges autophagic components with microtubules and mitochondria to affect autophagosomal biogenesis and degradation. Mitochondrion-associated protein LRPPRC functions as an inhibitor for autophagy initiation to ...
journal_title:Molecular carcinogenesis
pub_type: 杂志文章
doi:10.1002/mc.22193
更新日期:2015-10-01 00:00:00
abstract::Increasing evidence from recent research suggests a connection between cancer and a deranged equilibrium of histone acetylation, which is maintained by two competing enzymatic activities, histone acetyltransferases (HATs) and histone deacetylases (HDACs). It is our hypothesis that a significant proportion of leukemias...
journal_title:Molecular carcinogenesis
pub_type: 杂志文章
doi:10.1002/(sici)1098-2744(200004)27:4<268::aid-mc4>3
更新日期:2000-04-01 00:00:00
abstract::Signaling networks play important roles in cancer progression. For example, overexpression of the epidermal growth factor receptor (EGFR) is a poor prognostic indicator in multiple tumor types. Recent studies have postulated that the EGFR functions as a central conduit for signaling by different classes of cell surfac...
journal_title:Molecular carcinogenesis
pub_type: 杂志文章
doi:10.1002/mc.20138
更新日期:2005-12-01 00:00:00
abstract::The sequentiation of the human genome, together with the development of high throughput technologies, particularly gene-expression profiling, is giving us the opportunity to describe biological features in a quantitative manner. Here we review the use of global gene expression analyses in cancer research. Microarray a...
journal_title:Molecular carcinogenesis
pub_type: 杂志文章
doi:10.1002/mc.20430
更新日期:2008-08-01 00:00:00
abstract::Serially transplantable rat mammary tumor (RMT) cells are not dependent on exogenous epidermal growth factor (EGF) and insulin-like growth factor-I for continuous growth in serum-free medium. Previously, we found that conditioned medium obtained from these cells contained EGF-like mitogenic activity and stimulated tyr...
journal_title:Molecular carcinogenesis
pub_type: 杂志文章
doi:10.1002/(SICI)1098-2744(199602)15:2<134::AID-MC6>3
更新日期:1996-02-01 00:00:00
abstract::Despite effective surgical methods for non-melanoma skin cancer (NMSC), patients suffer from tissue damage, scarring, or even disfigurement; thus, there is a need for chemopreventive approaches. Because of the complex interplay between glucocorticoids (GCs), inflammation, and cancer, we sought to determine the role of...
journal_title:Molecular carcinogenesis
pub_type: 杂志文章
doi:10.1002/mc.22912
更新日期:2019-01-01 00:00:00
abstract::The number of validated biomarkers of tobacco smoke exposure is limited, and none exist for tobacco-related cancer. Additional biomarkers for smoke, effects on cellular systems in vivo are needed to improve early detection of lung cancer, and to assist the Food and Drug Administration in regulating exposures to tobacc...
journal_title:Molecular carcinogenesis
pub_type: 杂志文章
doi:10.1002/mc.22385
更新日期:2016-10-01 00:00:00