Regulation of CA 125 production by amnion and WISH cells in culture.

Abstract:

:Amnion and human amnion-derived WISH cells release CA 125 antigen into culture medium. CA 125 output was higher in WISH cells than in amnion cells. In this study we showed that release of the antigen is regulated, with both amnion and WISH cells responding in a similar manner to the tested agents. Release of CA 125 decreased in the presence of dexamethasone (10(-6) mol/L) or cycloheximide (0.1 micrograms/ml) and increased when colchicine (0.01 micrograms/ml) was added to the culture medium. Stimulatory and inhibitory effects were more apparent after 3 days in culture. The precise mechanisms by which some of these agents (colchicine and dexamethasone) affect CA 125 release remain unknown. We propose that amnion and WISH cells in culture represent a useful model to investigate some regulatory aspects of the production of CA 125 in normal tissues.

journal_name

Am J Obstet Gynecol

authors

Barbati A,Broccucci L,Anceschi MM,Cosmi EV

doi

10.1016/0002-9378(91)90713-2

subject

Has Abstract

pub_date

1991-05-01 00:00:00

pages

1355-9

issue

5 Pt 1

eissn

0002-9378

issn

1097-6868

pii

0002-9378(91)90713-2

journal_volume

164

pub_type

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