Abstract：
:We have demonstrated that vaccination of turkeys with an unformulated DNA vaccine induces significant protection against Chlamydophila (Cp.) psittaci infections. Nevertheless, the immunogenicity of the DNA vaccine can still be improved by increasing translation and transfection efficiency. Therefore, the ompA codon was adapted to the codon usage in birds, resulting in pcDNA1/MOMP(opt). To increase gene transfer, polyplexes of pcDNA1/MOMP(opt)-EGFP with different cationic polymers, such as linear and branched polyethyleneimine (lPEI and brPEI) and starburst PAMAM dendrimers, and lipoplexes with cationic DOTAP/DOPE liposomes were created. Transfection of lPEI and brPEI polyplexes with an N/P ratio of 8 resulted in the highest transfection efficiencies, but lPEI polyplexes were completely destroyed following nebulisation. Secondly, we examined the capacity of nebulised or intramuscularly (IM) administered brPEI-pcDNA1/MOMP(opt) to induce a significant protective immune response in SPF turkeys experimentally infected with 10(8) TCID(50) of a virulent Cp. psittaci strain. Results were compared to IM administration of naked plasmid DNA and to results of non-vaccinated animals. Intramuscular administration of brPEI-pcDNA1/MOMP(opt) increased the immunogenicity of the Cp. psittaci DNA vaccine as compared to IM administration of pcDNA1/MOMP(opt) or aerosol delivery of brPEI-pcDNA1/MOMP(opt). Improved immunogenicity was correlated with increased protection. Vaccinated groups were significantly protected against Cp. psittaci challenge.
journal_name
Vaccinejournal_title
Vaccineauthors
Verminnen K,Beeckman DS,Sanders NN,De Smedt S,Vanrompay DCdoi
10.1016/j.vaccine.2010.02.064subject
Has Abstractpub_date
2010-04-19 00:00:00pages
3095-105issue
18eissn
0264-410Xissn
1873-2518pii
S0264-410X(10)00245-8journal_volume
28pub_type
杂志文章相关文献
VACCINE文献大全abstract：:Using immunopotentiating, reconstituted influenza virosomes (IRIV) as a delivery vehicle, a number of vaccines have been developed. In humans, IRIV-based vaccines containing hepatitis A and influenza antigens have been found to possess enhanced immunogenicity compared to alum-adsorbed vaccine for hepatitis A or commer...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/s0264-410x(98)00440-x
更新日期：1999-03-26 00:00:00
abstract：BACKGROUND:A nasal influenza vaccine has been available only in a live attenuated form, which limits the range of recipients to immune-competent individuals. The present study evaluated a newly developed intranasal inactivated influenza vaccine with a novel adjuvant, heat-labile enterotoxin (LT) derived from E. coli (L...
journal_title：Vaccine
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.vaccine.2019.02.006
更新日期：2019-03-28 00:00:00
abstract：:Hepatitis E virus (HEV) is a major cause of enterically transmitted acute hepatitis of adults in developing nations. Our present studies show that, the complete ORF2 gene (1-660 amino acids, a.a.) coding for capsid protein of HEV as candidate DNA vaccine induced significant specific humoral and cellular immune respons...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2007.03.040
更新日期：2007-05-30 00:00:00
abstract：:The development of conjugate vaccines has enabled the prevention and control of Neisseria meningitidis caused by serogroups A, C, W-135 and Y. Vaccines that provide protection against a broad number of serogroup B strains likely will be available soon to enable greater control of meningococcal disease in high income c...
journal_title：Vaccine
pub_type： 杂志文章,评审
doi：10.1016/j.vaccine.2011.12.093
更新日期：2012-05-30 00:00:00
abstract：:The aim of the study was to analyze the impact of MenBvac, an outer membrane vesicle (OMV) vaccine against P1.7,16 strains, on meningococcal carriage. During a B:14:P1.7,16/ST-32 outbreak in Normandy (France), children aged 1-7 years were randomly selected to participate in the study. Among the 1082 volunteers, there ...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2013.06.080
更新日期：2013-09-13 00:00:00
abstract：:By the strategy "reverse vaccinology" a number of new antigens have been identified in Neisseria meningitidis, which are potential candidates for a highly needed broad-spectrum meningococcal vaccine. In the present study we examined the prevalence, sequence constancies and variations of the genes encoding three of the...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2005.11.006
更新日期：2006-03-15 00:00:00
abstract：:Chikungunya virus, transmitted by mosquitoes to man, causes an acute illness characterized by fever, rash and striking joint symptoms. US Military investigators developed, manufactured at The Salk Institute-Government Services Division (TSI-GSD), and tested the live, attenuated Chikungunya Vaccine TSI-GSD-218. The man...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2012.08.025
更新日期：2012-10-19 00:00:00
abstract：:Seasonal influenza vaccines represent a positive intervention to limit the spread of the virus and protect public health. Yet continual influenza evolution and its ability to evade immunity pose a constant threat. For these reasons, vaccines with improved potency and breadth of protection remain an important need. We ...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2019.08.030
更新日期：2019-09-30 00:00:00
abstract：:The authors trace the origins and history of anthrax and anthrax vaccines. They describe the aetiology and pathogenesis of the disease and the variety of symptoms which result from infection. The authors relate the early work performed by Pasteur, the development of existing vaccines and the efficacy of these vaccines...
journal_title：Vaccine
pub_type： 杂志文章,评审
doi：10.1016/0264-410x(84)90003-3
更新日期：1984-06-01 00:00:00
abstract：:To generate CD4+ T-helper cell lines, lymphocytes from HIV-seronegative subjects were primed in vitro with peptides derived from the V3 loop of HIV-1 gp120. Antigen-specific reactivity was inhibited by an anti-DR monoclonal antibody, indicating HLA-class II dependency, but peptides could be recognized in different HLA...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/0264-410x(94)90010-8
更新日期：1994-01-01 00:00:00
abstract：:Limited research has examined HPV vaccination in Appalachia, a region with cervical cancer disparities. We analyzed 2008-2010 National Immunization Survey-Teen data for adolescent females ages 13-17 from Appalachia (n=1951) to identify correlates of HPV vaccination and reasons why their parents do not intend to vaccin...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2013.04.068
更新日期：2013-06-28 00:00:00
abstract：OBJECTIVE:To assess the effectiveness of the monovalent rotavirus vaccine (RV1) to prevent rotavirus diarrhea admissions to emergency departments (ED) in Colombia. METHODS:A multicenter case-control study was carried out in six Colombian cities from 2011 to January, 2013. Cases were laboratory confirmed rotavirus diar...
journal_title：Vaccine
pub_type： 杂志文章,多中心研究
doi：10.1016/j.vaccine.2014.03.064
更新日期：2014-05-23 00:00:00
abstract：:A formalin-inactivated aluminium hydroxide adsorbed hepatitis A vaccine was evaluated in a dose-response study on 195 healthy male adults (age range: 18-31 years) in two French hospitals (Lyon, Rouen). Four doses (20, 40, 80, 160 RIA antigen units) were administered intramuscularly (i.m.) in two injections over a 6-mo...
journal_title：Vaccine
pub_type： 临床试验,杂志文章,多中心研究,随机对照试验
doi：10.1016/0264-410x(95)93140-5
更新日期：1995-02-01 00:00:00
abstract：:Japanese encephalitis (JE) is a fatal mosquito-borne disease that is vaccine-preventable. The natural infection rate is a critical factor for evaluations of the necessity for vaccination. Detection of antibodies to virus nonstructural (NS) proteins is a theoretical strategy to survey natural infections among populatio...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2009.09.064
更新日期：2009-11-23 00:00:00
abstract：:We previously observed that a detoxified Escherichia coli O111, Rc chemotype J5 lipopolysaccharide (J5dLPS)/group B meningococcal outer membrane protein (OMP) vaccine protected animals from experimental lethal sepsis when immune antibodies were given passively as treatment at the onset of fever or when vaccine was giv...
journal_title：Vaccine
pub_type： 临床试验,杂志文章
doi：10.1016/s0264-410x(03)00483-3
更新日期：2003-11-07 00:00:00
abstract：:Age related deterioration in the function of the immune system has been recognised in many species. The clinical presentations of such immune dysfunction are an age-related increased susceptibility to certain infections, and an increased incidence of autoimmune disease and certain cancers. Laboratory investigations re...
journal_title：Vaccine
pub_type： 杂志文章,评审
doi：10.1016/s0264-410x(99)00498-3
更新日期：2000-02-25 00:00:00
abstract：:The aim of this study was to assess the uptake of varicella vaccine in South Australian children under circumstances where varicella immunisation is recommended, but is not funded by Government. The study examined the main reasons that determined a parent's decision whether or not to have their child immunised with va...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2005.05.033
更新日期：2005-11-16 00:00:00
abstract：:Currently, infections of hand, foot and mouth disease (HFMD) due to Human Enterovirus 71 (EV71) cannot be prevented or treated, as there are no suitable vaccines or antiviral drugs. This study aimed to identify potential vaccine candidates for EV71 using in silico analysis of its viral capsid proteins. A combined in s...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2012.09.030
更新日期：2012-11-19 00:00:00
abstract：:In order to evaluate the long-term immunity 2 and 3 years after booster vaccination against tick-borne encephalitis (TBE) following time intervals 3 years and longer since last TBE immunization, 195 (mean age 50.5+/-15.1 years) and 240 subjects (mean age 47.0+/-15.4 years), respectively, who had received a single boos...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2007.01.116
更新日期：2007-06-28 00:00:00
abstract：:We sought to measure the anxiety felt by parents at the time of entry into a randomized controlled vaccine trial, and to determine if anxiety level was associated with parental demographic variables or past experience. The children were 2-month-old infants entering a randomized controlled clinical trial (RCT) of a dip...
journal_title：Vaccine
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1016/s0264-410x(03)00304-9
更新日期：2003-09-08 00:00:00
abstract：:DNA vaccines might offer an alternative to the live smallpox vaccine in providing protective efficacy in an orthopoxvirus (OPV) lethal respiratory challenge model. BALB/c mice were immunised with DNA vaccines coding for 10 different single vaccinia virus (VACV) membrane proteins. After an intranasal challenge with the...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2004.02.034
更新日期：2004-09-03 00:00:00
abstract：:Based on studies on the genetic and molecular basis of Shigella flexneri invasive properties, we have constructed and evaluated a double mutant of S. flexneri serotype 5 for utilization as a live attenuated oral vaccine against shigellosis. The first mutation, icsA, blocks intracellular spread of bacteria as well as c...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/0264-410x(89)90160-6
更新日期：1989-10-01 00:00:00
abstract：:Vaccines utilizing poorly immunogenic subunit antigens are dependent upon adjuvants to drive the appropriate T cell responses. In an effort to determine the ability of several adjuvants to promote cell-mediated immunity (CMI), we assessed delayed-type hypersensitivity (DTH) in mice inoculated with heat-killed Listeria...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/s0264-410x(96)00199-5
更新日期：1997-03-01 00:00:00
abstract：:Oral, replication-competent Ad-HIV vaccines are advancing to human trials. Previous evaluation of protective efficacy in non-human primates has primarily followed upper respiratory tract administrations. Here we compared sequential oral (O/O) versus intranasal/oral (I/O) priming of rhesus macaques with Ad5 host range ...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2007.09.017
更新日期：2007-11-19 00:00:00
abstract：:Experiments were carried out to examine the initial feasibility of immunizing infants and children with inactivated influenza virus vaccine combined with diphtheria-tetanus-pertussis (DTP) vaccine. Groups of mice were immunized with saline vaccine or vaccine mixed with DTP: three doses of vaccine were given 3 weeks ap...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/0264-410x(95)93310-6
更新日期：1995-02-01 00:00:00
abstract：:We demonstrated the feasibility of collecting and disseminating mid-season estimates of influenza vaccine coverage. We surveyed a nationally representative sample of U.S. adults (n = 3972) via the Internet about use of influenza vaccination as of mid-November 2008. Findings were presented on 10 December 2008 to the me...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2009.04.007
更新日期：2009-06-08 00:00:00
abstract：:A family of parasite antigens known as Plasmodium falciparum erythrocyte membrane protein 1 (PfEMP1) is believed to play an important role in the binding of infected erythrocytes to host receptors in the micro-vasculature. Available data advocates the existence of a subset of very adhesive (rosetting, auto-agglutinati...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2004.02.015
更新日期：2004-07-29 00:00:00
abstract：:Streptococcus pneumoniae is the leading cause of bacterial pneumonia, meningitis and sepsis in children worldwide. Despite available evidence on pneumococcal conjugate vaccine (PCV) impact on pneumonia hospitalizations in children, studies demonstrating PCV impact in morbidity and mortality in middle-income countries ...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2016.07.027
更新日期：2016-09-07 00:00:00
abstract：:Since 1996, the scientific community has become aware of 14 reports of yellow fever vaccine (YEL)-associated viscerotropic disease (YEL-AVD) cases and four reports of YEL-associated neurotropic disease (YEL-AND) worldwide, changing our understanding of the risks of the vaccine. Based on 722 adverse event reports after...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2005.01.089
更新日期：2005-05-09 00:00:00
abstract：:Although the theoretical concern of genetic recombination has been raised related to the use of live attenuated flavivirus vaccines [Seligman, Gould, Lancet 2004;363:2073-5], it has little foundation [e.g., Monath TP, Kanesa-Thasan N, Guirakhoo F, Pugachev K, Almond J, Lang J, et al. Vaccine 2005;23:2956-8]. To invest...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2007.07.016
更新日期：2007-09-17 00:00:00
