Abstract:
OBJECTIVE:The influence of cytomechanical forces in cellular migration, proliferation and differentiation of mesenchymal stem cells (MSCs) is still poorly understood in detail. METHODS:Human MSCs were isolated and cultivated onto the surface of a 3 x 3 mm porcine collagen I / III carrier. After incubation, cell cultures were transferred to the different cultures systems: regular static tissue flasks (group I), spinner flasks (group II) and rotating wall vessels (group III). Following standard protocols cells were stimulated lineage specific towards the osteogenic and chondrogenic lines. To evaluate the effects of applied cytomechanical forces towards cellular differentiation distinct parameters were measured (morphology, antigen and antigen expression) after a total cultivation period of 21 days in vitro. RESULTS:Depending on the cultivation technique we found significant differences in both gen and protein expression. CONCLUSION:Cytomechanical forces with rotational components strongly influence the osteogenic and chondrogenic differentiation.
journal_name
Eur J Med Resjournal_title
European journal of medical researchauthors
Zilkens C,Lögters T,Bittersohl B,Krauspe R,Lensing-Höhn S,Jäger Mdoi
10.1186/2047-783x-15-1-35subject
Has Abstractpub_date
2010-01-29 00:00:00pages
35-43issue
1eissn
0949-2321issn
2047-783Xjournal_volume
15pub_type
杂志文章abstract:BACKGROUND:Renal impairment is a common complication of multiple myeloma occurring in up to 50 % of patients at some stage in their disease. Due to occurrence of cast nephropathies we hypothesized circulatory dysregulation (vasoconstriction) in the kidneys with measurable elevation of the resistance index among these p...
journal_title:European journal of medical research
pub_type: 杂志文章
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abstract:OBJECTIVES:At workplaces, organic solvents are often used as mixtures. Nevertheless, there is limited knowledge of their acute effects on human central nervous system. Here we report the effects of a toluene-acetone mixture. METHODS:In a parallel design, subgroups of 12 healthy men each were exposed to a mixture conta...
journal_title:European journal of medical research
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更新日期:2005-09-12 00:00:00
abstract::Over the last years the reality surrounding HIV-infection has undergone a considerable change with regard to the life expectancy of patients, and the plans they can make for their lives. Because the majority of the HIV positive population is of reproductive age, one of these plans might be to have children, often as a...
journal_title:European journal of medical research
pub_type: 杂志文章
doi:
更新日期:2001-07-30 00:00:00
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journal_title:European journal of medical research
pub_type: 杂志文章
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journal_title:European journal of medical research
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更新日期:2017-10-18 00:00:00
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journal_title:European journal of medical research
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journal_title:European journal of medical research
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journal_title:European journal of medical research
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journal_title:European journal of medical research
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journal_title:European journal of medical research
pub_type: 杂志文章,meta分析,收录出版
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更新日期:2014-04-23 00:00:00
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journal_title:European journal of medical research
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journal_title:European journal of medical research
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doi:
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journal_title:European journal of medical research
pub_type: 临床试验,杂志文章,随机对照试验
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更新日期:2002-04-30 00:00:00
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更新日期:2017-11-15 00:00:00
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journal_title:European journal of medical research
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journal_title:European journal of medical research
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journal_title:European journal of medical research
pub_type: 杂志文章,评审
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journal_title:European journal of medical research
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更新日期:1997-08-28 00:00:00
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journal_title:European journal of medical research
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journal_title:European journal of medical research
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journal_title:European journal of medical research
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journal_title:European journal of medical research
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journal_title:European journal of medical research
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journal_title:European journal of medical research
pub_type: 杂志文章,评审
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journal_title:European journal of medical research
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更新日期:2019-07-22 00:00:00
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journal_title:European journal of medical research
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journal_title:European journal of medical research
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journal_title:European journal of medical research
pub_type: 杂志文章
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更新日期:2010-11-04 00:00:00