Abstract:
:A quick, reliable detection system is necessary to deal with bioterrorism. Loop-mediated isothermal amplification (LAMP) is a DNA amplification method that can amplify specific DNA fragments in isothermal conditions. We developed a new highly mobile and practical LAMP anthrax detection system that uses a disposable pocket warmer without the need for electricity (pocket-warmer LAMP). In our tests, the detection limit of the pocket-warmer LAMP was 1,000 copies of Bacillus anthracis pag and capB gene fragments per tube. The pocket-warmer LAMP also detected B. anthracis genes from DNA extracted from 0.1 volume of a B. anthracis colony. The lower detection limit of the pocket-warmer LAMP was not significantly different from that of a conventional LAMP using a heat block, and was not changed under cold (4 degrees C) or warm (37 degrees C) conditions in a Styrofoam box. The pocket-warmer LAMP could be useful against bioterrorism, and as a sensitive, reliable detection tool in areas with undependable electricity infrastructures.
journal_name
Jpn J Infect Disjournal_title
Japanese journal of infectious diseasesauthors
Hatano B,Maki T,Obara T,Fukumoto H,Hagisawa K,Matsushita Y,Okutani A,Bazartseren B,Inoue S,Sata T,Katano Hsubject
Has Abstractpub_date
2010-01-01 00:00:00pages
36-40issue
1eissn
1344-6304issn
1884-2836journal_volume
63pub_type
杂志文章abstract::Community-acquired pneumonia (CAP) can involve mixed-species bacterial infection. However, few studies have investigated antimicrobial efficacy in the treatment of mixed species infections. This study aimed to compare the in vivo antimicrobial activity of garenoxacin (GRNX) and levofloxacin (LVFX) against Streptococcu...
journal_title:Japanese journal of infectious diseases
pub_type: 杂志文章
doi:10.7883/yoken.JJID.2019.109
更新日期:2019-11-21 00:00:00
abstract::Chryseobacterium meningosepticum is an uncommon pathogen causing adult bacterial meningitis. Herein, we report the case history of one 21-year-old woman with this uncommon central nervous system infection. A diagnosis of adult C. meningosepticum meningitis can only be confirmed by a positive cerebrospinal fluid (CSF) ...
journal_title:Japanese journal of infectious diseases
pub_type: 杂志文章,评审
doi:
更新日期:2004-10-01 00:00:00
abstract::A total of 46 carbapenem- and multidrug-resistant (CR- and MDR-)Acinetobacter baumannii bacteremic isolates from a Taiwanese medical center were investigated over the period 2000 to 2006 using randomly amplified polymorphic DNA (RAPD) profiling and by analysing the genetic organization of their integrons. The results ...
journal_title:Japanese journal of infectious diseases
pub_type: 杂志文章
doi:
更新日期:2009-03-01 00:00:00
abstract::Measles is a highly contagious infection caused by the measles virus (MV). This study performed long-term surveillance in order to survey the prevalence of MV. A total of 417 patients diagnosed with or suspected of having measles were tested for MV between January 2007 and December 2016 in Osaka City, Japan. Reverse t...
journal_title:Japanese journal of infectious diseases
pub_type: 杂志文章
doi:10.7883/yoken.JJID.2017.322
更新日期:2018-03-22 00:00:00
abstract::Curtailing the observation of mice challenged with tetanus toxin in potency test of tetanus vaccine would reduce the agony of mice from spastic paralysis. From the viewpoint of animal welfare, we investigated the feasibility this measure. The potencies of 85 lots of vaccine obtained on the 4th day after challenge were...
journal_title:Japanese journal of infectious diseases
pub_type: 杂志文章
doi:
更新日期:2004-12-01 00:00:00
abstract::We present a case of cytomegalovirus (CMV)-induced pseudotumor of the gastric antrum. Although affliction of the entire gastrointestinal tract with CMV has been described, localization to the stomach and especially the gastric antrum is rare. Kaposi's sarcoma and non-Hodgkin's lymphoma are recognized causes of bowel t...
journal_title:Japanese journal of infectious diseases
pub_type: 杂志文章,评审
doi:
更新日期:2007-05-01 00:00:00
abstract::The aim of this study was to determine the capsular types of Haemophilus influenzae isolated from clinical specimens by slide agglutination serotyping (SAST) and PCR capsule typing methods. All the isolates were biotyped and their antibiotic resistance patterns also determined. Thirteen isolates of serotype b, 2 of se...
journal_title:Japanese journal of infectious diseases
pub_type: 杂志文章
doi:
更新日期:2011-01-01 00:00:00
abstract::Infection transmission systems circulate infection through complex contact patterns related to both contact patterns and patterns of factors that affect the risk of transmission given contact. The nonlinear dynamics of infection transmission cause these patterns to make big differences in population infection levels. ...
journal_title:Japanese journal of infectious diseases
pub_type: 杂志文章
doi:
更新日期:2005-12-01 00:00:00
abstract::We investigated the biological effect of hepatitis B virus (HBV) rtA181T/sW172* point mutation on HBsAg secretion and the potential mechanisms involved in hepatocarcinogenesis. Full-length HBV wild type (wt) and HBV rtA181T/sW172* expression plasmids were transfected into HepG2 cell lines or were injected into C57BL/6...
journal_title:Japanese journal of infectious diseases
pub_type: 杂志文章
doi:10.7883/yoken.JJID.2015.359
更新日期:2016-07-22 00:00:00
abstract::The aim of the present study was to compare serological tests (Rose Bengal [RB]; standard agglutination test [SAT]; enzyme immunoassay [EIA] for detection of IgM, IgA, and IgG; and 2-mercaptoethanol [2-ME] test) that are routinely used in patients prediagnosed with different clinical types of brucellosis (acute, subac...
journal_title:Japanese journal of infectious diseases
pub_type: 杂志文章
doi:
更新日期:2011-01-01 00:00:00
abstract::We previously reported human papillomavirus type 52 (HPV52) as the most prevalent high-risk genotype in non-cancer individuals in Vietnam. This study aimed to evaluate HPV genotypes and HPV16 E6 and E7 (E6/E7) gene variations in Vietnamese patients with genital cancers. Biopsy samples were collected from 124 Vietnames...
journal_title:Japanese journal of infectious diseases
pub_type: 杂志文章
doi:10.7883/yoken.JJID.2018.206
更新日期:2018-11-22 00:00:00
abstract::We previously reported a hospital-based epidemiological study on enterovirus (EV)-D68 infection among children during the autumn of 2015, which indirectly inferred an outbreak in Sendai, Japan. In this study, stocked sera of children (aged 0-6 years; without symptoms of infectious diseases) in the Sendai community col...
journal_title:Japanese journal of infectious diseases
pub_type: 杂志文章
doi:10.7883/yoken.JJID.2019.056
更新日期:2020-01-23 00:00:00
abstract::The objective of this study was to characterize the off-seasonal influenza virus A subtype H3N2, which caused an outbreak in an elderly hospital in Niigata, Japan. Virus isolates were subtyped by the hemagglutination-inhibition test and screened for antiviral drug sensitivity by real-time PCR using cycling probe techn...
journal_title:Japanese journal of infectious diseases
pub_type: 杂志文章
doi:
更新日期:2011-01-01 00:00:00
abstract::A rapid screen was developed for the detection of imipenem-resistant Acinetobacter baumanii (IRAb) following a recent outbreak in the Surgical Intensive Care Unit (SICU) of a hospital in Singapore. Antimicrobial solutions of imipenem ranging from 16 mg/L to 64 mg/L were prepared in-house. Each of the antimicrobial sol...
journal_title:Japanese journal of infectious diseases
pub_type: 杂志文章
doi:
更新日期:2005-02-01 00:00:00
abstract::We report a new case of Cardiobacterium hominis endocarditis identified during an acute coronary syndrome. The positivity of the blood cultures was confirmed rapidly (50 h) as a result of improvements to the automated detection system, whereby it is no longer necessary to incubate the vials for long periods of time wh...
journal_title:Japanese journal of infectious diseases
pub_type: 杂志文章
doi:
更新日期:2011-01-01 00:00:00
abstract::In the present study, we examined the prevalence of Providencia spp. in retail meats and in stool samples from diarrheal patients in Thailand. Providencia-specific (Psp)-PCR amplification was positive for 58% (15/26) of the chicken, 64% (16/25) of the pork, and 68% (17/25) of the beef samples. Subsequently, Providenci...
journal_title:Japanese journal of infectious diseases
pub_type: 杂志文章
doi:10.7883/yoken.JJID.2015.224
更新日期:2016-07-22 00:00:00
abstract::Human papillomavirus (HPV)-associated disease is common among men with HPV infection. A quadrivalent HPV (qHPV) vaccine has demonstrated 85.9% efficacy against HPV6/11/16/18-related, persistent (≥ 6 month) infection in a study of Japanese men aged 16-26 years old. Here, we report the results of an open-label study of ...
journal_title:Japanese journal of infectious diseases
pub_type: 杂志文章
doi:10.7883/yoken.JJID.2018.448
更新日期:2019-09-19 00:00:00
abstract::Ten of 140 patients (7.1%) showed negative results on a QFT-2G test. The 10 patients consisted of seven males and three females (mean age: 66.0 years old). Three of the 10 patients had no clinical symptoms and no underlying diseases. They showed negative results on the QFT-2G test repeatedly within 1 month and a negat...
journal_title:Japanese journal of infectious diseases
pub_type: 杂志文章
doi:
更新日期:2009-07-01 00:00:00
abstract::A total of 12 enterohemorrhagic Escherichia coli (EHEC) O157:H7 strains were isolated during a recent outbreak in a nursery school in Ehime Prefecture, Japan. These isolates were considered to be derived from a common strain when analyzed using an IS-printing method and pulsed-field gel electrophoresis. PCR analysis r...
journal_title:Japanese journal of infectious diseases
pub_type: 杂志文章
doi:10.7883/yoken.66.201
更新日期:2013-01-01 00:00:00
abstract::Recent evidence suggests that certain Fc gamma receptor alleles are genetic risk factors for infectious diseases. In this study, we evaluated Fc gamma RIIa polymorphism in patients with brucellosis. In a case-control study, the frequency of two alleles and three genotypes for Fc gamma RIIa were measured by PCR in 150 ...
journal_title:Japanese journal of infectious diseases
pub_type: 杂志文章
doi:
更新日期:2007-07-01 00:00:00
abstract::The aim of the present study was to evaluate the predation efficacy of Bdellovibrio bacteriovorus on multidrug-resistant (MDR) or extensive drug resistant (XDR) gram-negative pathogens and their corresponding biofilms. In this study, we examined the ability of B. bacteriovorus to prey on MDR and XDR gram-negative clin...
journal_title:Japanese journal of infectious diseases
pub_type: 杂志文章
doi:10.7883/yoken.JJID.2016.405
更新日期:2017-09-25 00:00:00
abstract::We report on a hospitalized patient with 2019 novel coronavirus disease whose fecal samples tested negative 22 days after respiratory samples tested negative, highlighting that the duration of viral shedding is longer than that previously expected. Current clinical examinations for treatment and discharge standards ar...
journal_title:Japanese journal of infectious diseases
pub_type: 杂志文章
doi:10.7883/yoken.JJID.2020.265
更新日期:2021-01-22 00:00:00
abstract::Systemic candidiasis is a significant cause of morbidity and mortality in patients with hematologic disorders. The aim of this study was to determine the prevalence of systemic candidiasis and the efficiency of the polymerase chain reaction-enzyme-linked immunosorbent assay (PCR-ELIZA) method for the early detection o...
journal_title:Japanese journal of infectious diseases
pub_type: 杂志文章
doi:
更新日期:2009-01-01 00:00:00
abstract::This study was conducted to investigate the distribution of rotavirus genotypes in Nara Prefecture, Japan before and after the introduction of rotavirus vaccination in 2011. Since the 2011/2012 season, DS-1-like G1P[8] strains have been detected in Nara Prefecture, accounting for about half of all strains in the 2014/...
journal_title:Japanese journal of infectious diseases
pub_type: 杂志文章
doi:10.7883/yoken.JJID.2016.419
更新日期:2017-09-25 00:00:00
abstract::To clarify the molecular epidemiology of human metapneumovirus (HMPV) in Okinawa Prefecture, located in a subtropical region of Japan, we performed genetic analysis of the F gene in HMPV from patients with acute respiratory infection from January 2009 to December 2011. HMPV was detected in 18 of 485 throat swabs (3...
journal_title:Japanese journal of infectious diseases
pub_type: 杂志文章
doi:10.7883/yoken.65.337
更新日期:2012-07-01 00:00:00
abstract::Staphylococcus lugdunensis (SL) is a bacterium with a highly pathogenicity than most other coagulase-negative Staphylococcus spp. (CoNS). In Japan, data on this pathogen are sparse, and the current prevalence of SL bacteremia is unknown. Therefore, we investigated the prevalence of SL in blood culture specimens in a p...
journal_title:Japanese journal of infectious diseases
pub_type: 杂志文章,多中心研究
doi:10.7883/yoken.JJID.2016.130
更新日期:2017-07-24 00:00:00
abstract::We examined 29 isolates of Salmonella enterica subspecies enterica serovar Schwarzengrund from broiler chickens (n=19) and retail chicken meats (n=10) in Japan for antimicrobial susceptibility and pulsed-field gel electrophoresis (PFGE) profiling. All isolates exhibited resistance to both bicozamycin and sulfadimethox...
journal_title:Japanese journal of infectious diseases
pub_type: 杂志文章
doi:
更新日期:2009-05-01 00:00:00
abstract::Vibrio vulnificus is a Gram-negative bacterium which is associated with severe infections in humans. We experienced two cases of sucrose-fermenting V. vulnificus infection. The causative agents in both cases were unidentifiable by conventional identification systems because of their unique characteristics, and sequenc...
journal_title:Japanese journal of infectious diseases
pub_type: 杂志文章
doi:
更新日期:2006-04-01 00:00:00
abstract::We compared the overall usage of veterinary therapeutic antimicrobials in Japan to the proportion of antimicrobial-resistant. Escherichia coli isolated from the feces of apparently healthy food-producing animals in 2001. The annual sales volume of veterinary antimicrobials, which is published and accumulated informati...
journal_title:Japanese journal of infectious diseases
pub_type: 杂志文章
doi:
更新日期:2005-12-01 00:00:00
abstract::The prevalence and intensity of Loa loa and Mansonella perstans were studied in three villages of the Ijebu north area in Ogun State, western Nigeria. Blood samples were collected by finger-pricking from 373 (181 males, 192 females) subjects with an age range of 4 to 55 years. The blood samples were examined microscop...
journal_title:Japanese journal of infectious diseases
pub_type: 杂志文章
doi:
更新日期:2001-06-01 00:00:00