Abstract:
:A total of 12 enterohemorrhagic Escherichia coli (EHEC) O157:H7 strains were isolated during a recent outbreak in a nursery school in Ehime Prefecture, Japan. These isolates were considered to be derived from a common strain when analyzed using an IS-printing method and pulsed-field gel electrophoresis. PCR analysis revealed that the isolates harbor stx1, stx2, eae, and hlyA. However, assessment of the production of the Stx proteins revealed that these isolates produced Stx1 but not Stx2. We determined their stx2 variants such as stx2c and found that the size of the PCR product was much larger than the expected size. Sequencing of the entire stx2 gene revealed that a 1310-bp fragment was inserted into the coding region of the Stx2A subunit and that the sequences of the insert were identical to those of IS1203v. According to the sequences around the insertion site, additional amino acid residues should be attached at the C-terminus of the A subunit, which may hamper the Stx2 complex formation. Finally, this study also suggested that such an insertion may lead to the misinterpretation of results when screening EHEC isolates for virulence genes by PCR.
journal_name
Jpn J Infect Disjournal_title
Japanese journal of infectious diseasesauthors
Asano Y,Karasudani T,Tanaka H,Matsumoto J,Okada M,Nakamura K,Kondo H,Shinomiya Hdoi
10.7883/yoken.66.201subject
Has Abstractpub_date
2013-01-01 00:00:00pages
201-6issue
3eissn
1344-6304issn
1884-2836pii
DN/JST.JSTAGE/yoken/66.201journal_volume
66pub_type
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