Abstract：
:There have recently been large outbreaks of Marburg hemorrhagic fever (MHF) caused by Marburgvirus (MARV) in the Democratic Republic of Congo and Angola. The development of reliable diagnostic systems for MHF is urgently needed. An antigen-capture enzyme-linked immunosorbent assay (Ag-capture ELISA) using either of the two monoclonal antibodies (2A7 and 2H6) produced by immunizing mice with recombinant nucleoprotein of MARV was described (Journal of Medical Virology, 76, 111-118, 2005). In the present study, it was revealed that the Ag-capture ELISA specifically detected authentic MARV antigen and that the sensitivity of the Ag-capture ELISA was at a level similar to that of reverse-transcription polymerase chain reaction. These results suggest that the Ag-capture ELISA using the monoclonal antibodies, 2A7 and 2H6, is applicable to the diagnosis of MHF.
journal_name
Jpn J Infect Disjournal_title
Japanese journal of infectious diseasesauthors
Saijo M,Georges-Courbot MC,Fukushi S,Mizutani T,Philippe M,Georges AJ,Kurane I,Morikawa Ssubject
Has Abstractpub_date
2006-10-01 00:00:00pages
323-5issue
5eissn
1344-6304issn
1884-2836journal_volume
59pub_type
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journal_title：Japanese journal of infectious diseases
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