Abstract：
:The growth and amino acid utilization of a mouse macrophage-like cell line J774.1 was investigated in two different culture media supplemented with 10% fetal bovine serum (FBS). The J774.1 cells grew faster, and consumed glutamine and serine at higher rates in DMEM than in RPMI1640 medium. The consumption of other amino acids was much less, while considerable quantities of alanine, glutamic acid and glycine were produced by the J774.1 cells. When the cells became confluent, serine, but not glutamine, was nearly depleted from the culture medium, followed by cell death characterized by smear DNA fragmentation, slight caspase-3 activation and structural damage of the mitochondria. Serine is required for the growth of mouse macrophage-like cell lines, and DMEM is superior to RPMI1640 for long-term cell culture.
journal_name
Anticancer Resjournal_title
Anticancer researchauthors
Thet MM,Yokote Y,Chu Q,Wang QT,Kishino K,Tamura N,Shimada J,Amano O,Kanda Y,Kunii S,Takekawa F,Hatano H,Shimada C,Hasegawa H,Sakagami Hsubject
Has Abstractpub_date
2009-12-01 00:00:00pages
5083-8issue
12eissn
0250-7005issn
1791-7530pii
29/12/5083journal_volume
29pub_type
杂志文章abstract：BACKGROUND:18F-FDG positron emission tomography (PET) may alter therapeutic management of patients with cervical cancer because 18F-FDG-positive pelvic and para-aortic lymph nodes are associated with lower progression-free survival and vice versa. However, the accuracy of 18F-FDG PET might be impaired by a preceding ly...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2003-05-01 00:00:00
abstract：BACKGROUND:The mammalian target of rapamycin (mTOR) controls cell growth through protein synthesis regulation. It can be activated by protein kinase B (AKT) or through ribosomal S6 kinase (S6K1). In malignant glioma, mTOR inhibitors have antiproliferative and proapoptotic effects and mTOR has been suggested as a target...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2009-08-01 00:00:00
abstract：AIM:To describe the feasibility, safety, and oncological outcomes of a modified triple-incision total radical vulvectomy and inguino-femoral lymphadenectomy in patients with locally advanced squamous vulvar cancer. PATIENTS AND METHODS:A modified triple-incision technique performed by two surgical teams operating simu...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2014-12-01 00:00:00
abstract：BACKGROUND:Granulosa cell tumor of the testis is a rare intermediate stromal cell tumor that can be distinguished in the adult and juvenile type. The juvenile type is the most common reason for scrotal swelling in newborns under the age of six months. Less than fifty cases of this disease entity have been reported in t...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2010-05-01 00:00:00
abstract：BACKGROUND:The chronomodulated infusion of 5-FU, FA and oxaliplatin allows a significant increase in dose intensity and antitumor efficacy in patients with metastatic colorectal cancer. Here we investigated if substitution of oxaliplatin with cisplatin produced a similar antitumor activity in previously untreated patie...
journal_title：Anticancer research
pub_type： 临床试验,杂志文章
doi：
更新日期：2000-03-01 00:00:00
abstract：BACKGROUND/AIM:Even though prostate cancer (PCa) has good prognosis, there is a discrepancy in the risk among ethnic groups, with high morbidity in African American men. Single nucleotide polymorphisms (SNPs) in interleukin 10 (IL-10) have been associated with inflammation and cancer risk. We investigated the associati...
journal_title：Anticancer research
pub_type： 杂志文章
doi：10.21873/anticanres.13923
更新日期：2020-01-01 00:00:00
abstract：AIM:Neoadjuvant epirubicin/docetaxel (ET) combination chemotherapy was administered to breast cancer patients in order to investigate their clinical and pathological response. Moreover, the breast-conserving surgery (BCS) rate, disease-free (DFS) and overall survival (OS), safety profile and the correlation of biologic...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2012-08-01 00:00:00
abstract：BACKGROUND:Expression of novel stem cell-associated marker human embryonic stem cell 77 (HES77) was studied in rectal neuroendocrine tumors (NETs), which comprise 10 to 15% of gastroenteropancreatic NETs, some with metastatic potential. MATERIALS AND METHODS:WHO 2010 classification was applied, and immunohistochemical...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2015-07-01 00:00:00
abstract：BACKGROUND:In this study, the effect of novel taxane SB-T-1216 and paclitaxel on sensitive MDA-MB-435 and resistant NCI/ADR-RES human breast cancer cells was compared. MATERIALS AND METHODS:Cell growth and survival were evaluated after 96-hour incubation with tested concentrations of taxanes. The effect on the formati...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2009-08-01 00:00:00
abstract：:Considerable progress has recently been achieved in understanding the pathogenesis of human immunodeficiency virus type 1 (HIV-1), and in improving the efficacy of antiretroviral therapies for the treatment of patients with the acquired immunodeficiency syndrome (AIDS). The pharmacological properties of new drugs are ...
journal_title：Anticancer research
pub_type： 杂志文章,评审
doi：
更新日期：1998-07-01 00:00:00
abstract：:Oral squamous cell carcinoma (OSCC) is the sixth most common malignancy in humans including type I diabetic and normal rats. Tobacco and alcohol, as well as dysregulation of oncogenes and tumor suppressor genes, epigenetic changes and mitochondrial mutations have been implicated in OSCC development. Recent epidemiolog...
journal_title：Anticancer research
pub_type： 杂志文章,评审
doi：
更新日期：2007-11-01 00:00:00
abstract：:Some new phenothiazines have been synthesized on the basis of previous studies. The anticancer activity of "half-mustard type" phenothiazines was investigated on sixty different cancer cell lines in vitro. The percentage of growth (PG), 50% inhibition of growth (GI50), the tumor growth inhibition (TGI) and the concent...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1998-01-01 00:00:00
abstract：BACKGROUND/AIM:In the last years, the use of Image Guided Stereotactic Radiotherapy (IG-SBRT) in patients with metastatic prostate cancer has increased. In this study, we aimed to assess the role of IG-SBRT in terms of local control and safety in patients with metastatic prostate cancer. MATERIALS AND METHODS:Primary ...
journal_title：Anticancer research
pub_type： 杂志文章
doi：10.21873/anticanres.12572
更新日期：2018-05-01 00:00:00
abstract：UNLABELLED:The aim of this study was to investigate the apoptosis resulting from NSC 95397, brefeldin A, bortezomib and sanguinarine in neuroendocrine tumor cell lines. MATERIALS AND METHODS:A multiparametric high-content screening assay for measurement of apoptosis was used. The human pancreatic carcinoid cell line, ...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2010-01-01 00:00:00
abstract：BACKGROUND/AIM:The Cancer Stem Cell (CSC) model proposes that cancer is driven by a cellular component which possesses stem cell (SC) properties, cancer stem cells (CSCs), a distinct cell-type which is tumorigenic and capable of invasion and metastasis. Enzymatic activity of aldehyde dehydrogenase-1 (ALDH1), a de-toxif...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2014-03-01 00:00:00
abstract：AIM:The aim of the study was to identify novel biomarkers that are vital for improving management of patients with gastric cancer (GC). MATERIALS AND METHODS:An RNA-sequencing analysis was conducted using gastric tissue from patients with metastatic GC. In vitro cell functions were evaluated by siRNA-mediated knockdow...
journal_title：Anticancer research
pub_type： 杂志文章
doi：10.21873/anticanres.13820
更新日期：2019-11-01 00:00:00
abstract：:Berlin-Druckrey (BD-IV) rats were used to evaluate the level of neoplastic transformation, initiation of DNA damage and repair following treatment with N-ethyl-N-nitrosourea (ENU). ENU, a potent neurocarcinogen in the Sprague-Dawley (CD) and BDIX rat strains, was a less potent neurocarcinogen when administered to 30 d...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1986-01-01 00:00:00
abstract：UNLABELLED:The pharmacology of progestins includes actions initiated by various cellular targets, including classic receptors characterized as nuclear transcription factors (nPR), G-protein-coupled membrane receptors (mPR), enzymes, membrane channels and transporters. The effects initiated by targets other than nPR are...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2010-12-01 00:00:00
abstract：:CA 72-4 is a high molecular weight, pancarcinoma human tumor mucin which may play an important role in the identification (i.e., staging) and clinical management of patients with gastric carcinoma. In the present study of 242 patients with primary or recurrent gastric cancer, a higher percentage of these patients had ...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1996-07-01 00:00:00
abstract：:L1210 cell lines selected for resistance to deoxyadenosine exhibit altered steady-state levels of the mRNA for the early response genes and p53. In the deoxyadenosine-resistant cell lines (Y8 and ED2), the levels of the mRNAs for p53 and c-jun were markedly decreased while the steady-state levels for mRNAs for c-myc, ...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1996-11-01 00:00:00
abstract：:A new cell line, designated CC2/CUHK2, was established from a squamous carcinoma of the uterine cervix from a Chinese patient. The cell line grew well without interruption for over 12 months and over 90 passages. The doubling time of CC2/CUHK2 was 72 hours at passage 57. When seeded at a density of 1.5 x 10(3) cells p...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1993-07-01 00:00:00
abstract：BACKGROUND:This report presents results of a study delineating changing trends in radical external beam radiotherapy usage for prostate cancer between the 1996-1998 and 1999-2001 Patterns of Care Study (PCS) survey periods in Japan. MATERIALS AND METHODS:Out of the 694 patients comprising the 1996-1998 and 1999-2001 P...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2005-09-01 00:00:00
abstract：:Telomeres, the tandem-repeated hexamers at the termini of mammalian chromosomes, form protective complexes in association with specific proteins that together with telomerase, a specialised telomere-synthesizing enzyme, regulate telomere length. Telomere shortening is associated with cellular senescence and is implica...
journal_title：Anticancer research
pub_type： 杂志文章,评审
doi：
更新日期：2005-07-01 00:00:00
abstract：BACKGROUND:The presence of cytokeratin (CK)-positive cells in the bone marrow (BM) of breast cancer patients is an independent prognostic marker for long-term survival. While the exact nature of these cells remains under investigation, their persistence after chemotherapy is linked to decreased survival, making them a ...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2005-05-01 00:00:00
abstract：UNLABELLED:The aim of the present study was to investigate hypoxia-inducible factor 1α (HIF-1α) and glucose transporter-1 (GLUT-1) expressions as predictors of response and survival after chemoradiotherapy in pretreatment biopsy specimens from patients with rectal cancer. MATERIALS AND METHODS:The immunohistological e...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2011-05-01 00:00:00
abstract：AIM:The purpose of the present study was the analysis of the relationship between tumor necrosis factor-α (TNF-α) -308 G/A and cervical intraepithelial neoplasia (CIN). MATERIALS AND METHODS:A prospective case-control study was performed, enrolling 78 cases of cervical intraepithelial neoplasia and 107 controls. Every...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2014-01-01 00:00:00
abstract：BACKGROUND:Positron emission tomography-computed tomography (PET-CT) is currently not established in the management of recurrent ovarian cancer. Here, its value in diagnosis and therapy planning was evaluated. PATIENTS AND METHODS:Seventy patients received PET-CT for suspicion of recurrent ovarian cancer. PET-CT and s...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2008-07-01 00:00:00
abstract：:A new transitional cell carcinoma cell line, BCCA-1, derived from a primary urinary bladder carcinoma, was characterized with respect to the growth patterns of in vitro culture, xenotransplantability in SCID mice and immunophenotypic profile. The most unusual finding was a strong tendency of forming many aggregates (m...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2000-03-01 00:00:00
abstract：:Metallothioneins (MT) are low molecular weight cysteine-rich proteins, present in a wide variety of eukaryotes. Although their physiological function is not entirely understood, recently it was found that in vitro human MTs (hMTs) expression prevents apoptosis. In the present study, the apoptosis preventing effect of ...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2000-11-01 00:00:00
abstract：BACKGROUND:Nucleotide-binding domain and leucine-rich repeat-containing family, pyrin domain-containing 7 (NLRP7) (pyrin-containing apoptotic protease activating factor-1-like protein 3; PYPAF3, NACHT domain-, leucine-rich repeat, and pyrin domain-containing 7; NALP7) has been thought to contribute to innate immunity a...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2008-07-01 00:00:00
