Abstract:
:The cytotoxicity of methotrexate (MTX) on representative human tumour cell lines (two cell lines from head and neck carcinomas, two from breast carcinomas, two from osteosarcomas and one lymphoblastoid cell line) was evaluated to: (1) examine the optimal time interval between MTX and folinic acid (FA) administration; (2) determine the critical FA/MTX concentration ratios; and (3) compare the relative effects of the equimolar mixture d,I-FA and I-FA. The cytotoxic effects of MTX were assessed by the MTT semi-automated test. For all of the cell lines tested, a significant inverse relationship was noted between the degree of MTX cytotoxicity reversal and the duration of the time interval between MTX and FA administration. Overall an 18-24 h interval between MTX and FA represented a time-threshold after which MTX effects could not efficiently be reversed by FA in most cell lines. With shorter time intervals between MTX and FA, MTX cytotoxicity could be partially on even totally reversed by FA; the intensity of reversal varied among the cell lines tested, and depended on the FA/MTX ratio. Regardless of the interval between MTX and FA, analysis of the various FA/MTX ratios revealed a significant direct relationship between this ratio and the percentage of recovery. Presence of the d-form had no influence on the MTX rescue capacity of the I-form; this suggests that the presence of the d-FA is unlikely to have any significant clinical consequences.
journal_name
Br J Cancerjournal_title
British journal of cancerauthors
Bernard S,Etienne MC,Fischel JL,Formento P,Milano Gdoi
10.1038/bjc.1991.70subject
Has Abstractpub_date
1991-02-01 00:00:00pages
303-7issue
2eissn
0007-0920issn
1532-1827journal_volume
63pub_type
杂志文章abstract::HER2/neu-derived peptides inducing MHC class II-restricted CD4+ T helper lymphocyte (Th) responses, although critical for tumour rejection, are not thoroughly characterized. Here, we report the generation and characterization of CD4+ T cell clones specifically recognizing a HER-2/neu-derived peptide (776-788) [designa...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1054/bjoc.2001.2089
更新日期:2001-11-16 00:00:00
abstract::This study explored the relationships of serum insulin-like growth factors, IGF-I and IGF-II, and their binding proteins (IGFBP)-2 and IGFBP-3, with key clinicopathological parameters in 92 patients with colorectal cancer (cases). Comparisons were made with 57 individuals who had a normal colonoscopy (controls). Seria...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1054/bjoc.2000.1462
更新日期:2000-11-01 00:00:00
abstract::We determined the prevalence of type-specific hrHPV infections in the Netherlands on cervical scrapes of 45 362 women aged 18-65 years. The overall hrHPV prevalence peaked at the age of 22 with peak prevalence of 24%. Each of the 14 hrHPV types decreased significantly with age (P-values between 0.0009 and 0.03). The p...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/sj.bjc.6604162
更新日期:2008-02-12 00:00:00
abstract::MDM2 is an oncoprotein that inhibits p53 tumour-suppressor protein. Amplification of the MDM2 gene and overexpression of its protein have been observed in some human malignancies, and these abnormalities have a role in tumorigenesis through inactivation of p53 function. To determine the clinicopathological and prognos...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1997.221
更新日期:1997-01-01 00:00:00
abstract::The haemodynamics of the uterine arteries and myometrium were assessed using Doppler ultrasound in forty consecutive patients requiring treatment for invasive mole and choriocarcinoma. The investigations were performed prior to the commencement of chemotherapy and the subjects followed prospectively. The Doppler wavef...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1992.379
更新日期:1992-11-01 00:00:00
abstract::As part of an NHS Executive Trent regional initiative we considered the role and cost-effectiveness of high dose chemotherapy in the treatment of relapsed Hodgkin's disease and non-Hodgkin's lymphoma. The key trials and case series show an additional patient benefit of 0.8-1.1 life years over standard chemotherapy. We...
journal_title:British journal of cancer
pub_type: 杂志文章,meta分析
doi:10.1054/bjoc.1999.0880
更新日期:2000-01-01 00:00:00
abstract:BACKGROUND:In the coBRIM study, cobimetinib plus vemurafenib (C+V) significantly improved survival outcomes vs placebo and vemurafenib (P+V) in patients with advanced/metastatic BRAFV600-mutated melanoma. An analysis of health-related quality of life (HRQOL) from coBRIM is reported. METHODS:Patients completing the Eur...
journal_title:British journal of cancer
pub_type: 杂志文章,多中心研究,随机对照试验
doi:10.1038/bjc.2017.488
更新日期:2018-03-20 00:00:00
abstract::Aminoglutethimide has been used in combination with hydrocortisone in patients with advanced prostatic cancer with the rationale that it causes a 'medical adrenalectomy'. Both objective and subjective responses have been recorded. We have examined the effect of AG and HC alone and in combination on plasma androgen lev...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1988.40
更新日期:1988-02-01 00:00:00
abstract::Human primary thyroid follicular epithelial cells were transfected with a plasmid containing an origin-defective SV40 genome (SVori-) to produce several immortal cell lines. Two of the 10 cell lines analysed expressed specific features of thyroid epithelial function (iodide-trapping and thyroglobulin production). Thes...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1989.387
更新日期:1989-12-01 00:00:00
abstract::Human peripheral-blood mononuclear cells, separated by Isopaque-Ficoll flotation and E-rosette formation, were tested by the fluorescein fluorescence polarization method of Cercek & Cercek (the SCM test). The response to stimulation with PHA or cancer tissue leads to a decreased polarization value TP). The responding ...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1979.228
更新日期:1979-10-01 00:00:00
abstract::Preclinical studies have demonstrated the synergistic anti-tumour activity of combination therapy with the oral cytostatics, 5'-deoxy-5-fluorouridine (5'-DFUR) and cyclophosphamide (CPA), in human breast cancer xenograft models. This study was performed to evaluate the efficacy and safety of this oral combination chem...
journal_title:British journal of cancer
pub_type: 临床试验,杂志文章
doi:10.1038/sj.bjc.6601350
更新日期:2003-11-03 00:00:00
abstract::In addition to the direct targeting effects on HER2-positive cells, trastuzumab may have a therapeutic role modulating the activity of the cellular immune system in patients with breast cancer. To investigate this further, the balance of T-regulatory (T(reg)), Th17, natural killer (NK) and NK T (NKT) cells before, dur...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/sj.bjc.6604963
更新日期:2009-04-07 00:00:00
abstract::Lines of fibroblasts have been established from normal individuals and from patients diagnosed as suffering from one of the mucopolysaccharidoses or mucopolysaccharide-storage diseases. Transformation of these lines with SV40 virus has been found to reduce their capacity to secrete sulphated mucopolysaccharides into t...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1977.156
更新日期:1977-07-01 00:00:00
abstract::Two transplantable, highly immunogenic syngeneic C57BL fibrosarcomas, FS1 and FS6, were shown to have tumour-specific rejection antigens, as shown by excision of the primary tumours and i.p. or i.m. injection of graded doses of the specific and unrelated tumour cells. I.p. challenge with tumour cells induced a large a...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1978.253
更新日期:1978-11-01 00:00:00
abstract::In a case-control study using a large UK primary care database, we found that non-steroidal anti-inflammatory drugs had no protective effect against biliary carcinomas (cholangiocarcinoma and gall bladder cancer). Increased risks were observed for cigarette smoking, diabetes, gallstone disease and obesity. ...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/sj.bjc.6604765
更新日期:2009-01-13 00:00:00
abstract::The formation of cyclic AMP was studied in normal liver, subcutaneous hepatomas derived from MH1C1 cells, and premalignant liver and primary hepatomas induced by the carcinogens 2-acetylaminofluorene (AAF) and 4-dimethylamino-azobenzene (DAB). While only very slight effects of prostaglandins (PG) were seen in slices o...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1978.281
更新日期:1978-12-01 00:00:00
abstract::Variants of the melanocortin-1 receptor (MC1R) gene have been linked to sun-sensitive skin types and hair colour, and may independently play a role in susceptibility to cutaneous melanoma. To assess the role of MC1R variants in uveal melanoma, we have analysed a cohort of 350 patients for the changes within the major ...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/sj.bjc.6601358
更新日期:2003-11-17 00:00:00
abstract::Evidence in the literature suggests that hyperthermia (HT) or inhibitors of calmodulin can increase the sensitivity of rodent cells to bleomycin (BLM) by interfering with DNA repair functions. In an attempt to explore methods of improving the efficacy of thermochemotherapy we have investigated the individual and combi...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1986.14
更新日期:1986-01-01 00:00:00
abstract:BACKGROUND:Though p53 mutations are rare in ES, there is a strong indication that p53 mutant tumours form a particularly bad prognostic group. As such, novel treatment strategies are warranted that would specifically target and eradicate tumour cells containing mutant p53 in this subset of ES patients. METHODS:PRIMA-1...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.2013.635
更新日期:2013-11-12 00:00:00
abstract::In adults, the haemolytic-uraemic syndrome (HUS) is associated with probable causative factors in the minority of all cases. Cytotoxic drugs are one of these potential causative agents. Although metastatic cancer by itself is a recognized risk-factor for the development of HUS, therapy with mitomycin-C, with cis-plati...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/sj.bjc.6690242
更新日期:1999-03-01 00:00:00
abstract::The life span of C57/Bl mice inoculated with Lewis lung carcinoma cells was prolonged if the mice were pre-immunized with membranes from these cells infected in vitro with influenza virus. Likewise, BALB/c mice were protected against the malignant tumour WEHI-11 by prior immunization with extracts of cultured WEHI-11 ...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1975.106
更新日期:1975-06-01 00:00:00
abstract::Sera from 182 newly diagnosed breast cancer patients were assayed for antibodies to p53 using an enzyme-linked immunosorbent assay (ELISA) method, and antibodies were detected in 48 (26%) compared with 1 out of 76 (1.3%) normal control volunteers (P = 0.0001). In breast cancer patients, autoantibodies were found in al...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1994.219
更新日期:1994-06-01 00:00:00
abstract::Epidemiology has shown an association between exposure to extremely low frequency (ELF) electromagnetic fields (EMF) and childhood leukaemia. The causal nature and biological basis of this association are however questionable. Studies with aneuploid cell lines raised the hypothesis that ELF EMF may act as a coleukaemo...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/sj.bjc.6601010
更新日期:2003-06-16 00:00:00
abstract::5-fluorouracil (5FU) is activated intracellularly to cytotoxic 5-fluoronucleotides (FNuct). These were detected non-invasively in rats bearing the Walker carcinosarcoma by 19F-magnetic resonance spectroscopy (MRS) following an i.v. bolus dose of 5FU (50 mg kg-1). Pre-treatment of the rats (3 to 24 h earlier) by methot...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1992.75
更新日期:1992-03-01 00:00:00
abstract::Angiogenesis is an essential requirement for tumour growth and metastasis and is regulated by a complex network of factors produced by both stromal cells and neoplastic cells within solid tumours. The cytokine tumour necrosis factor alpha (TNF-alpha) and the enzyme thymidine phosphorylase (TP) are two factors known to...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1998.373
更新日期:1998-06-01 00:00:00
abstract::Dormant and regressing implants of C3H mammary carcinoma MC2 were always found to be surrounded by a cellular fibrous capsule where macrophages and T cells predominated as the cellular elements. Macrophages were always closely associated with the collagen deposition, and stained with anti-collagen type I immuno-peroxi...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1991.169
更新日期:1991-05-01 00:00:00
abstract::Chinese hamster V79-171B spheroids implanted in the peritoneal cavity of C3H mice were characterized as a model for evaluating the toxicity of drugs requiring metabolic activation in vivo. After 24 hours in the peritoneal cavity, spheroid cellularity and plating efficiency were not significantly decreased, and host ce...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1987.196
更新日期:1987-09-01 00:00:00
abstract::In the past decade, an increasing number of frequently positive randomised clinical trials have been completed, allowing new consideration of the present therapeutic armamentarium for advanced renal cell carcinoma. These studies were predominantly designed to compare the experimental drugs with 1 of 2 active control a...
journal_title:British journal of cancer
pub_type: 杂志文章,评审
doi:10.1038/bjc.2014.240
更新日期:2015-01-06 00:00:00
abstract::Febrile neutropenia (FN) causes significant morbidity and mortality in patients receiving cytotoxic chemotherapy and can lead to reduced chemotherapy dose intensity and increased overall treatment costs. Antibiotic prophylaxis reduces the incidence of FN. Recent research and meta-analyses confirm that prophylactic flu...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/sj.bjc.6605270
更新日期:2009-09-01 00:00:00
abstract:BACKGROUND:MYCN amplification with subsequent MYCN protein overexpression is a powerful indicator of poor prognosis of neuroblastoma patients. Little is known regarding the prognostic significance of the homologous MYC protein expression in neuroblastoma. METHODS:Immunostaining for MYCN and MYC protein was performed o...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.2015.188
更新日期:2015-06-30 00:00:00